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The map label for this gene is pdtaS [H]

Identifier: 86750140

GI number: 86750140

Start: 3445749

End: 3446834

Strand: Direct

Name: pdtaS [H]

Synonym: RPB_3023

Alternate gene names: 86750140

Gene position: 3445749-3446834 (Clockwise)

Preceding gene: 86750139

Following gene: 86750141

Centisome position: 64.63

GC content: 66.48

Gene sequence:

>1086_bases
ATGACTCCTGCTGCTGCGCCGACTCTGCTGTATATCGACGACGACGAGGCGCTCGGGCGCCTGGTCTCACGCGGCCTGAA
GCGACAGGGTTTCTGCGTCGAGCACGTGCTGAGCGGCGAGGCGGGGCTGGAGCGATTGCGCAAGGGCGGCGTCGACGTGG
TGGCGCTGGATCAGTACATGCCCGGCCTCGACGGGCTGGAAACGCTGGAGCAGATCCAGAACATTCCGGATGCGCCGCCG
GTGGTCTTCGTTACCGCATCGCAGGATTCCAGCATCGCCGTGACCGCGCTGAAGGCCGGCGCCGCGGATTATCTGGTGAA
AGATACCCAGGGCGACTTCGTCCCCCTGCTCCAGGTCGCCGCCGATCAGGCGCTGCGCCAAGCCGTGATCCGCAAGGCCC
GCGACGACGCCGAAGCCGAAGTCCGCGCCTCGCGTGATCGCTATGCGGCGCTGGCCGCAGAGCGCGAAATGCTGCTGCGC
GAGGTCAACCACCGGGTCGGCAATTCGCTGCAGATCATCGCGTCGCTGCTGCATCTGCAGGCTTCGTCCAGCAAGGAGAG
CGAGGTCAAGGCGGCGCTCACCAACGCGATGGGCCGCGTCGCTGCGGTGGCGCAAGTCCACCGCCGGCTCTACACCTCGC
ACGACCTCAAGAGCGTGATGCTGAACCAGTATCTCGAAGCGTTGCTCGAGGATTTGCGTCGTTCGGCCGAAGGCAACCGG
ATGTCGCGCCTGACGCTCGATGCGGAGGCGGTCGAGATCGATCCCGACCGCGCGGTCGCGATCGGCATCGTCGTCAACGA
GTTGGTGATGAACGCGGTGAAGTACGCCTATCCGGAAGGCAAGGGCCCGATCCACGTGGTGCTGAAGGCCCAGGGCGACG
ACCTCGAACTCGCAATTTCCGATGACGGCGTCGGTCTTGCGGTCAAGGCCAATCCGCACTCGACCGGCATGGGCCAGCGC
ATCGTCAATGCGATGGCGACCAAGCTCGACGCCAGCGTCGAGCGCGACCCCGCGCATGCCGGCACCCGCATGGTGCTGCG
ATTCCGCCGCACCAACATCGCCGCGCCGACGCCGGCCATCGCGTAG

Upstream 100 bases:

>100_bases
TCTACATCACCAAGCCGGTGAACTACGAGAGCTTCGCCAACGCGATCCGTCAGCTCGGCCTGTTCTTTTCCGTGATCCAG
GTCCCTCAAACCGAGGCTCC

Downstream 100 bases:

>100_bases
TCGGCCGCCCCGCCAAATCGGGCCACGAATCCGGCCGCACTATCCCAAAGCCGCCTTGCCATTTTGCCGGAGCTCGGGCA
GGTTCCGCGCGCGCCGCTGC

Product: signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTPAAAPTLLYIDDDEALGRLVSRGLKRQGFCVEHVLSGEAGLERLRKGGVDVVALDQYMPGLDGLETLEQIQNIPDAPP
VVFVTASQDSSIAVTALKAGAADYLVKDTQGDFVPLLQVAADQALRQAVIRKARDDAEAEVRASRDRYAALAAEREMLLR
EVNHRVGNSLQIIASLLHLQASSSKESEVKAALTNAMGRVAAVAQVHRRLYTSHDLKSVMLNQYLEALLEDLRRSAEGNR
MSRLTLDAEAVEIDPDRAVAIGIVVNELVMNAVKYAYPEGKGPIHVVLKAQGDDLELAISDDGVGLAVKANPHSTGMGQR
IVNAMATKLDASVERDPAHAGTRMVLRFRRTNIAAPTPAIA

Sequences:

>Translated_361_residues
MTPAAAPTLLYIDDDEALGRLVSRGLKRQGFCVEHVLSGEAGLERLRKGGVDVVALDQYMPGLDGLETLEQIQNIPDAPP
VVFVTASQDSSIAVTALKAGAADYLVKDTQGDFVPLLQVAADQALRQAVIRKARDDAEAEVRASRDRYAALAAEREMLLR
EVNHRVGNSLQIIASLLHLQASSSKESEVKAALTNAMGRVAAVAQVHRRLYTSHDLKSVMLNQYLEALLEDLRRSAEGNR
MSRLTLDAEAVEIDPDRAVAIGIVVNELVMNAVKYAYPEGKGPIHVVLKAQGDDLELAISDDGVGLAVKANPHSTGMGQR
IVNAMATKLDASVERDPAHAGTRMVLRFRRTNIAAPTPAIA
>Mature_360_residues
TPAAAPTLLYIDDDEALGRLVSRGLKRQGFCVEHVLSGEAGLERLRKGGVDVVALDQYMPGLDGLETLEQIQNIPDAPPV
VFVTASQDSSIAVTALKAGAADYLVKDTQGDFVPLLQVAADQALRQAVIRKARDDAEAEVRASRDRYAALAAEREMLLRE
VNHRVGNSLQIIASLLHLQASSSKESEVKAALTNAMGRVAAVAQVHRRLYTSHDLKSVMLNQYLEALLEDLRRSAEGNRM
SRLTLDAEAVEIDPDRAVAIGIVVNELVMNAVKYAYPEGKGPIHVVLKAQGDDLELAISDDGVGLAVKANPHSTGMGQRI
VNAMATKLDASVERDPAHAGTRMVLRFRRTNIAAPTPAIA

Specific function: Member of the two-component regulatory system pdtaR/pdtaS. Autophosphorylates, probably on a histidine residue, and transfers its phosphate group to pdtaR [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789402, Length=152, Percent_Identity=32.2368421052632, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=35.8974358974359, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=99, Percent_Identity=32.3232323232323, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR022066
- InterPro:   IPR011495
- InterPro:   IPR005467 [H]

Pfam domain/function: PF12282 H_kinase_N; PF02518 HATPase_c; PF07568 HisKA_2 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38861; Mature: 38730

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAAAPTLLYIDDDEALGRLVSRGLKRQGFCVEHVLSGEAGLERLRKGGVDVVALDQYM
CCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCEEEHHHHC
PGLDGLETLEQIQNIPDAPPVVFVTASQDSSIAVTALKAGAADYLVKDTQGDFVPLLQVA
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCHHHEEECCCCCHHHHHHHH
ADQALRQAVIRKARDDAEAEVRASRDRYAALAAEREMLLREVNHRVGNSLQIIASLLHLQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ASSSKESEVKAALTNAMGRVAAVAQVHRRLYTSHDLKSVMLNQYLEALLEDLRRSAEGNR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MSRLTLDAEAVEIDPDRAVAIGIVVNELVMNAVKYAYPEGKGPIHVVLKAQGDDLELAIS
EEEEEECCEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEC
DDGVGLAVKANPHSTGMGQRIVNAMATKLDASVERDPAHAGTRMVLRFRRTNIAAPTPAI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
A
C
>Mature Secondary Structure 
TPAAAPTLLYIDDDEALGRLVSRGLKRQGFCVEHVLSGEAGLERLRKGGVDVVALDQYM
CCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCEEEHHHHC
PGLDGLETLEQIQNIPDAPPVVFVTASQDSSIAVTALKAGAADYLVKDTQGDFVPLLQVA
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCHHHEEECCCCCHHHHHHHH
ADQALRQAVIRKARDDAEAEVRASRDRYAALAAEREMLLREVNHRVGNSLQIIASLLHLQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ASSSKESEVKAALTNAMGRVAAVAQVHRRLYTSHDLKSVMLNQYLEALLEDLRRSAEGNR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MSRLTLDAEAVEIDPDRAVAIGIVVNELVMNAVKYAYPEGKGPIHVVLKAQGDDLELAIS
EEEEEECCEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEC
DDGVGLAVKANPHSTGMGQRIVNAMATKLDASVERDPAHAGTRMVLRFRRTNIAAPTPAI
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]