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The map label for this gene is phbC [H]

Identifier: 86750086

GI number: 86750086

Start: 3380804

End: 3382609

Strand: Direct

Name: phbC [H]

Synonym: RPB_2969

Alternate gene names: 86750086

Gene position: 3380804-3382609 (Clockwise)

Preceding gene: 86750077

Following gene: 86750091

Centisome position: 63.41

GC content: 63.46

Gene sequence:

>1806_bases
ATGAGCGACATGCTACCAGAAACCAGCACGGCGAAGAAGTTCGATCCCGAGGCTTTCGCGCGTAATCTGGCGCAGGCGAT
GGAGTGCGGCAGCCAGGCGCTGGCGACGATGCTGAAGCCGAGCGAGCCGATCGACGGTGTCAAGCCGCCGACCGAACTGA
CGGAGATCGTCAAGACGCTCACCAGCGTCGCCACCTACTGGATGTCCGACCAGACCCGCGCCGCGGAGCTGCAGACGAAG
CTCGGCAAATCCTATCTCGAGCTGTGGGGCAACGCCTCCAAGAAGCTCGCCGGCGAGACGGCCGTCGCCCCGACCATCGA
GCCGCCGGCGCGCGACAAGCGTTTCGCCGATCCGGACTGGCGAAAGAACCAGTTCTACGAATTCATCATGCAGGCCTATC
TGTTGACCTCGCAATGGGCCAACGAGCTGGTCGAGAAGGCCGACGGCCTCGACCCGCACACCAAGCGAAAGGCCGCATTC
TACATCCAGCAGATCACCAACGCGATGGCGCCGTCGAACTTCGTGCCGACCAATCCGGAATTGACCCGGCTGACGCTCGA
GAACAGCGGCGACAATCTGGTGCGCGGCATGAAGATGCTCGCCGAGGACATCGCCGCCGGCAAAGGCCATCTCAAGATTC
GCCAGTCCGACCCGTCCAATCTGGAAGTCGGCGTCAACATGGCGACGACGCCCGGCAAGGTGATCTTCCAGAACGAGATC
ATGCAACTGATCCAGTACACGCCCGCGACCGAGACGGTGCTGCGCACGCCTCTGCTGATCGTGCCGCCATGGATCAACAA
GTTCTACATTCTCGATCTCAAGCCCGAGAAGTCGTTCATCAAATACTGCGTCGACCAGGGTCTCACCGTGTTCGTGATCT
CCTGGGTCAATCCCGACAAGCGCCTCGCCGACAAGAGCTTCGCCGACTACATGAAGCTCGGGCCGCTGACCGCGATGGAC
GTGATCGAGAAGGTCACCGGCGAGCTGAAGGTGCACACCATCGGCTATTGCGTCGGCGGCACCCTGCTCGCCTCGACGCT
GGCCTGGCTGGCCGAGCGCCGCCGCCAGCGCGTCACCTCGGCGACCTTCCTGACCACCCAGGTCGATTTCACCCATGCCG
GCGACCTCAGCGTGTTCGTCGACGAGGGCCAGATCTCGGCGCTGGAGCGCGACATGCAGACGACCGGCGTGCTCGAAGGC
GCCAGGATGGCGATGGCGTTCAACATGCTGCGGTCGAACGACCTGATCTGGTCCTATGTGGTCAGCAACTATCTGAAGGG
CCAGCCGCCCGCCGCGTTCGACCTGCTGCACTGGAATTCCGACGCCACCCGGATGCCGGCGGCGAACCACTCTTACTATC
TGCGCAACTGTTATCTCGACAACAAGCTGTCGGCCGGCACCATGGTGCTCGACGACACCCCGCTCGATCTGTCGAAGGTC
AAGGTGCCGATCTACAACCTCGCCACCCGCGAGGACCACATCGCGCCGGCGGAATCCGTGCTGTACGGTTCGCAATTCTT
CGGCGGCCCGGTGAAATACGTGCTGTCCGGCTCCGGCCACATCGCCGGCGTGATCAATCCGCCGGCGGCGAACAAATATC
AGTACTGGACCAGCGAGACCATCCGCGCCGGCTCGGTCGCCGAATGGCTGAAGGGCGCGCAGGAGCACAAGGGTTCGTGG
TGGCCGGACTGGCGCGAATGGGTCGGCAGTTTCGATCCCGAACAGGTCCCTGCCCGCGGCATCGGCAACGAGACCTATCA
GCCGATCGAGGACGCCCCCGGCAGCTACGTCCGGGTGCGATCCTGA

Upstream 100 bases:

>100_bases
CAGCACATCGAGTTCAGCACCGTTTACCGAACTATGCCATCATCGGCGAGACAACGACAAAATCGTCTAAGGTCTTCGAA
CAGGGTTGAAGCGTCCGGTC

Downstream 100 bases:

>100_bases
GCGGCGGTCGCGAATAGACGGCGGCTCAGGTCCCCGACACCCAGGCCAGCACCAGCGCGAAGCCGGCGAGGCCCACGGTG
ACGTGGGTGCCGAGCAGCAC

Product: Poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MSDMLPETSTAKKFDPEAFARNLAQAMECGSQALATMLKPSEPIDGVKPPTELTEIVKTLTSVATYWMSDQTRAAELQTK
LGKSYLELWGNASKKLAGETAVAPTIEPPARDKRFADPDWRKNQFYEFIMQAYLLTSQWANELVEKADGLDPHTKRKAAF
YIQQITNAMAPSNFVPTNPELTRLTLENSGDNLVRGMKMLAEDIAAGKGHLKIRQSDPSNLEVGVNMATTPGKVIFQNEI
MQLIQYTPATETVLRTPLLIVPPWINKFYILDLKPEKSFIKYCVDQGLTVFVISWVNPDKRLADKSFADYMKLGPLTAMD
VIEKVTGELKVHTIGYCVGGTLLASTLAWLAERRRQRVTSATFLTTQVDFTHAGDLSVFVDEGQISALERDMQTTGVLEG
ARMAMAFNMLRSNDLIWSYVVSNYLKGQPPAAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDDTPLDLSKV
KVPIYNLATREDHIAPAESVLYGSQFFGGPVKYVLSGSGHIAGVINPPAANKYQYWTSETIRAGSVAEWLKGAQEHKGSW
WPDWREWVGSFDPEQVPARGIGNETYQPIEDAPGSYVRVRS

Sequences:

>Translated_601_residues
MSDMLPETSTAKKFDPEAFARNLAQAMECGSQALATMLKPSEPIDGVKPPTELTEIVKTLTSVATYWMSDQTRAAELQTK
LGKSYLELWGNASKKLAGETAVAPTIEPPARDKRFADPDWRKNQFYEFIMQAYLLTSQWANELVEKADGLDPHTKRKAAF
YIQQITNAMAPSNFVPTNPELTRLTLENSGDNLVRGMKMLAEDIAAGKGHLKIRQSDPSNLEVGVNMATTPGKVIFQNEI
MQLIQYTPATETVLRTPLLIVPPWINKFYILDLKPEKSFIKYCVDQGLTVFVISWVNPDKRLADKSFADYMKLGPLTAMD
VIEKVTGELKVHTIGYCVGGTLLASTLAWLAERRRQRVTSATFLTTQVDFTHAGDLSVFVDEGQISALERDMQTTGVLEG
ARMAMAFNMLRSNDLIWSYVVSNYLKGQPPAAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDDTPLDLSKV
KVPIYNLATREDHIAPAESVLYGSQFFGGPVKYVLSGSGHIAGVINPPAANKYQYWTSETIRAGSVAEWLKGAQEHKGSW
WPDWREWVGSFDPEQVPARGIGNETYQPIEDAPGSYVRVRS
>Mature_600_residues
SDMLPETSTAKKFDPEAFARNLAQAMECGSQALATMLKPSEPIDGVKPPTELTEIVKTLTSVATYWMSDQTRAAELQTKL
GKSYLELWGNASKKLAGETAVAPTIEPPARDKRFADPDWRKNQFYEFIMQAYLLTSQWANELVEKADGLDPHTKRKAAFY
IQQITNAMAPSNFVPTNPELTRLTLENSGDNLVRGMKMLAEDIAAGKGHLKIRQSDPSNLEVGVNMATTPGKVIFQNEIM
QLIQYTPATETVLRTPLLIVPPWINKFYILDLKPEKSFIKYCVDQGLTVFVISWVNPDKRLADKSFADYMKLGPLTAMDV
IEKVTGELKVHTIGYCVGGTLLASTLAWLAERRRQRVTSATFLTTQVDFTHAGDLSVFVDEGQISALERDMQTTGVLEGA
RMAMAFNMLRSNDLIWSYVVSNYLKGQPPAAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDDTPLDLSKVK
VPIYNLATREDHIAPAESVLYGSQFFGGPVKYVLSGSGHIAGVINPPAANKYQYWTSETIRAGSVAEWLKGAQEHKGSWW
PDWREWVGSFDPEQVPARGIGNETYQPIEDAPGSYVRVRS

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 66961; Mature: 66830

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMLPETSTAKKFDPEAFARNLAQAMECGSQALATMLKPSEPIDGVKPPTELTEIVKTL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
TSVATYWMSDQTRAAELQTKLGKSYLELWGNASKKLAGETAVAPTIEPPARDKRFADPDW
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
RKNQFYEFIMQAYLLTSQWANELVEKADGLDPHTKRKAAFYIQQITNAMAPSNFVPTNPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
LTRLTLENSGDNLVRGMKMLAEDIAAGKGHLKIRQSDPSNLEVGVNMATTPGKVIFQNEI
EEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEHHHH
MQLIQYTPATETVLRTPLLIVPPWINKFYILDLKPEKSFIKYCVDQGLTVFVISWVNPDK
HHHHHCCCCHHHHHHCCCEEECCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCHH
RLADKSFADYMKLGPLTAMDVIEKVTGELKVHTIGYCVGGTLLASTLAWLAERRRQRVTS
HHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFLTTQVDFTHAGDLSVFVDEGQISALERDMQTTGVLEGARMAMAFNMLRSNDLIWSYV
HEEEEEEECEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VSNYLKGQPPAAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDDTPLDLSKV
HHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCCHHHE
KVPIYNLATREDHIAPAESVLYGSQFFGGPVKYVLSGSGHIAGVINPPAANKYQYWTSET
EEEHEECCCCCCCCCCHHHHHCCHHHCCCCEEEEECCCCCEEEEECCCCCCCEEEECCCH
IRAGSVAEWLKGAQEHKGSWWPDWREWVGSFDPEQVPARGIGNETYQPIEDAPGSYVRVR
HCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEC
S
C
>Mature Secondary Structure 
SDMLPETSTAKKFDPEAFARNLAQAMECGSQALATMLKPSEPIDGVKPPTELTEIVKTL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
TSVATYWMSDQTRAAELQTKLGKSYLELWGNASKKLAGETAVAPTIEPPARDKRFADPDW
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
RKNQFYEFIMQAYLLTSQWANELVEKADGLDPHTKRKAAFYIQQITNAMAPSNFVPTNPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
LTRLTLENSGDNLVRGMKMLAEDIAAGKGHLKIRQSDPSNLEVGVNMATTPGKVIFQNEI
EEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEHHHH
MQLIQYTPATETVLRTPLLIVPPWINKFYILDLKPEKSFIKYCVDQGLTVFVISWVNPDK
HHHHHCCCCHHHHHHCCCEEECCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCHH
RLADKSFADYMKLGPLTAMDVIEKVTGELKVHTIGYCVGGTLLASTLAWLAERRRQRVTS
HHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFLTTQVDFTHAGDLSVFVDEGQISALERDMQTTGVLEGARMAMAFNMLRSNDLIWSYV
HEEEEEEECEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VSNYLKGQPPAAFDLLHWNSDATRMPAANHSYYLRNCYLDNKLSAGTMVLDDTPLDLSKV
HHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCCHHHE
KVPIYNLATREDHIAPAESVLYGSQFFGGPVKYVLSGSGHIAGVINPPAANKYQYWTSET
EEEHEECCCCCCCCCCHHHHHCCHHHCCCCEEEEECCCCCEEEEECCCCCCCEEEECCCH
IRAGSVAEWLKGAQEHKGSWWPDWREWVGSFDPEQVPARGIGNETYQPIEDAPGSYVRVR
HCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]