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The map label for this gene is ispDF

Identifier: 86750002

GI number: 86750002

Start: 3285487

End: 3286692

Strand: Direct

Name: ispDF

Synonym: RPB_2885

Alternate gene names: 86750002

Gene position: 3285487-3286692 (Clockwise)

Preceding gene: 86749992

Following gene: 86750003

Centisome position: 61.62

GC content: 70.4

Gene sequence:

>1206_bases
ATGCAGAAACCTCCCCGTACCGCCGCCATCATCGTCGCCGCCGGCCGCGGCCTCCGCGCTGGCGCGGGCGGCCCCAAACA
ATACCGCACGCTCGCCGGACGGCCGGTGATTGCCAGGGCTTTGCAGCCGTTTTGTACGCACCCGGAGGTCTTTGCGGTCC
AGCCCGTGACCAATCCGGACGACACCGCGACGTTCAACGAGGCGGTCGCCGGGCTCGATTTCCGACCTGCGGTCGGTGGC
GGCGCGACCCGGCAGGCGTCGGTTCGCGCCGGGCTGGAAGCGCTGGCCGAACTGAACCCCGACATCGTGCTGATCCACGA
CGCGGCGCGTCCCTTTGTCACGCCGGATCTGATCTCGCGTGCGATCGTCGCGGCCGGCCAGACCGGCGCGGCGCTGCCGG
TGATCGCGGTCAACGACACCGTCAAGCAGGTCGATGCCGAGGGCTGCGTCGTGGCGACGCCGGACCGCGCGCAATTGCGG
ATCGCGCAGACGCCGCAGGCCTTCCGTTTCGACGTGATTCTCGACGCGCACCGCCGCGCCGCGCGCGATGGTCGCGATGA
CTTCACCGACGACGCCGCGATCGCCGAATGGGCGGGATTGACGGTGTCCACGTTCGAGGGCGATGCTGCCAACATGAAAC
TGACCACGCCGGAAGACTTCTCGCGCGAAGAGAGCCGCCTCACCGCGGCGCTCGGCGACATCCGCACCGGCACCGGCTAC
GACGTCCACGCCTTCGGCGACGGCGATCACGTCTGGCTGTGCGGGCTGAAGGTGCCGCACAATCGCGGCTTCCTGGCGCA
TTCCGACGGCGACGTCGGACTGCACGCGCTGGTCGACGCCATCCTCGGCGCGCTGGCCGACGGCGACATCGGCTCGCACT
TCCCGCCGACCGACCCGCAATGGAAGGGCGCGGCCTCCGACAAGTTCCTGAAATACGCGGTCGACCGCGTCGCCGCGCGC
GGCGGCCGCATCGCCAATCTCGAAGTGACGATGATCTGCGAGCGGCCGAAGATCGGCCCGCTGCGCGACCCGATGCGGCA
GCGCATCGCCGAGATCACCGGCGTTCCGGTGTCGCGCGTCGCCGTGAAGGCGACCACCAGCGAGCGGCTCGGCTTCACCG
GCCGCGAGGAAGGCATCGCCGCCACCGCGTCGGCCACCATCCGGCTGCCTTGGTCCCCTTGGGGCGCCGAAGGACAGGCC
TCCTGA

Upstream 100 bases:

>100_bases
CTGACGGCGCGCGAATCACGCGGAATCGACGCTCAGGCATGCTAAGGGCTCTCCCCGCCCCCTCGCCTGCTGTCCCCGAT
CGAGAAACTGATCCTTGCCG

Downstream 100 bases:

>100_bases
CGATGAGCGGCAACGACGCCCGCACCGATTCCCGTGCCTTGGCCCGCTCGTTGCTCGACCTCTGCCGCTCGCGCAAGCTC
ACCATCGCCACGGCTGAATC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MQKPPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARALQPFCTHPEVFAVQPVTNPDDTATFNEAVAGLDFRPAVGG
GATRQASVRAGLEALAELNPDIVLIHDAARPFVTPDLISRAIVAAGQTGAALPVIAVNDTVKQVDAEGCVVATPDRAQLR
IAQTPQAFRFDVILDAHRRAARDGRDDFTDDAAIAEWAGLTVSTFEGDAANMKLTTPEDFSREESRLTAALGDIRTGTGY
DVHAFGDGDHVWLCGLKVPHNRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQWKGAASDKFLKYAVDRVAAR
GGRIANLEVTMICERPKIGPLRDPMRQRIAEITGVPVSRVAVKATTSERLGFTGREEGIAATASATIRLPWSPWGAEGQA
S

Sequences:

>Translated_401_residues
MQKPPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARALQPFCTHPEVFAVQPVTNPDDTATFNEAVAGLDFRPAVGG
GATRQASVRAGLEALAELNPDIVLIHDAARPFVTPDLISRAIVAAGQTGAALPVIAVNDTVKQVDAEGCVVATPDRAQLR
IAQTPQAFRFDVILDAHRRAARDGRDDFTDDAAIAEWAGLTVSTFEGDAANMKLTTPEDFSREESRLTAALGDIRTGTGY
DVHAFGDGDHVWLCGLKVPHNRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQWKGAASDKFLKYAVDRVAAR
GGRIANLEVTMICERPKIGPLRDPMRQRIAEITGVPVSRVAVKATTSERLGFTGREEGIAATASATIRLPWSPWGAEGQA
S
>Mature_401_residues
MQKPPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARALQPFCTHPEVFAVQPVTNPDDTATFNEAVAGLDFRPAVGG
GATRQASVRAGLEALAELNPDIVLIHDAARPFVTPDLISRAIVAAGQTGAALPVIAVNDTVKQVDAEGCVVATPDRAQLR
IAQTPQAFRFDVILDAHRRAARDGRDDFTDDAAIAEWAGLTVSTFEGDAANMKLTTPEDFSREESRLTAALGDIRTGTGY
DVHAFGDGDHVWLCGLKVPHNRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQWKGAASDKFLKYAVDRVAAR
GGRIANLEVTMICERPKIGPLRDPMRQRIAEITGVPVSRVAVKATTSERLGFTGREEGIAATASATIRLPWSPWGAEGQA
S

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=232, Percent_Identity=29.7413793103448, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=51.9480519480519, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1789104, Length=224, Percent_Identity=33.4821428571429, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_RHOP2 (Q2IW23)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486498.1
- ProteinModelPortal:   Q2IW23
- SMR:   Q2IW23
- STRING:   Q2IW23
- GeneID:   3910679
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2885
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- ProtClustDB:   CLSK911224
- BioCyc:   RPAL316058:RPB_2885-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 42361; Mature: 42361

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKPPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARALQPFCTHPEVFAVQPVTNPD
CCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCC
DTATFNEAVAGLDFRPAVGGGATRQASVRAGLEALAELNPDIVLIHDAARPFVTPDLISR
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
AIVAAGQTGAALPVIAVNDTVKQVDAEGCVVATPDRAQLRIAQTPQAFRFDVILDAHRRA
HHHHCCCCCCEEEEEEECCHHHHHCCCCCEEECCCCCEEEEECCCCCEEEEEEEHHHHHH
ARDGRDDFTDDAAIAEWAGLTVSTFEGDAANMKLTTPEDFSREESRLTAALGDIRTGTGY
HCCCCCCCCCCHHHHHHCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCC
DVHAFGDGDHVWLCGLKVPHNRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQ
EEEEECCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
WKGAASDKFLKYAVDRVAARGGRIANLEVTMICERPKIGPLRDPMRQRIAEITGVPVSRV
CCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHE
AVKATTSERLGFTGREEGIAATASATIRLPWSPWGAEGQAS
EEEECCHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MQKPPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARALQPFCTHPEVFAVQPVTNPD
CCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCC
DTATFNEAVAGLDFRPAVGGGATRQASVRAGLEALAELNPDIVLIHDAARPFVTPDLISR
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
AIVAAGQTGAALPVIAVNDTVKQVDAEGCVVATPDRAQLRIAQTPQAFRFDVILDAHRRA
HHHHCCCCCCEEEEEEECCHHHHHCCCCCEEECCCCCEEEEECCCCCEEEEEEEHHHHHH
ARDGRDDFTDDAAIAEWAGLTVSTFEGDAANMKLTTPEDFSREESRLTAALGDIRTGTGY
HCCCCCCCCCCHHHHHHCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCC
DVHAFGDGDHVWLCGLKVPHNRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQ
EEEEECCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
WKGAASDKFLKYAVDRVAARGGRIANLEVTMICERPKIGPLRDPMRQRIAEITGVPVSRV
CCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHE
AVKATTSERLGFTGREEGIAATASATIRLPWSPWGAEGQAS
EEEECCHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA