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The map label for this gene is recG [H]

Identifier: 86749969

GI number: 86749969

Start: 3249096

End: 3251282

Strand: Direct

Name: recG [H]

Synonym: RPB_2852

Alternate gene names: 86749969

Gene position: 3249096-3251282 (Clockwise)

Preceding gene: 86749966

Following gene: 86749973

Centisome position: 60.94

GC content: 68.77

Gene sequence:

>2187_bases
ATGTCAGCCCGCCGCGGCGCATTGCAATTGGCGCAAAATGCATCCAAGGCTGCGGGTCACATCCGCCCCAATCGAGCCGG
CCCGATGCGTCCCGCTTTGCTCAATCCCCTGTTCGCCCCGGTCACCGGCCTCACCGGCGTCGGTCCGAAGCAGGACAAGC
TGTTTCGCTATCTGCTGGATCGCGACGACACCCCGCGGCTGGCCGATCTGCTGTTGCATCTGCCGGTGAGCGTGATCGAT
CGCCGCTCGCGGCCCAAGATCCGCGACGCCCAGCCCGGCACCGTGGTGACGCTGGAAGTCACCGTCGACCGCCACCGCCC
GCCACCACCCGGTCGCTCACGCGCGCCCTATCTGGTCTATGCCAGCGACGAGACCGGCGACGTCATCCTGACCTTCTTCC
GCGCCAAGCCGGAGTTCGTCGAGAAGCTGCTGCCGGTCGGCGCCAAGCGCTACGTCTCCGGCACCGGCCAGCTGTATGAC
GGCACGCTGCAGATCGTGCATCCCGACCGCGTCGTCGACGAGGAAGGCTTCGCCAAGCTGCCGAAGATCGACCCGGTGTA
TCCGCTCACCGAGGGGCTGGCGATCGGCTCGCTACGCCGCGCGGTGGCGCAGGCGCTGACCAGACTGCCGGCGCTGCCGG
AATGGATCAGTCCCGAGGTGCTGCGACGCTGCAGCTTTCCCGCCTTCGCCGAGGCACTGCAGCGCGTCCATATCCCGCAT
GAGCCGACCGACATCCTGCCCGACGGCCCGTTCTGGTCGCGCCTCGCCTATGACGAATTGCTCGCCGGACAATTGGCGCT
GGCGCTGGTGCGGGCACAGCTCCGTCGCCCGGCCGGCACGCGCAATGCCGGCGACGGCCGGCTGCGCCACAAGATCATCG
ACGCGCTGCCTTATGCGCTGACGGCCTCGCAACAGGCCGCTGCCGCCGCGATCGCGGAGGATCTGCAGCAACCTGTGCGG
ATGCTGCGGCTGCTGCAGGGCGACGTCGGCTCCGGCAAGACCGTGGTGGCGCTGCTGGCCGCCGCCGCCGTCGCCGAGGC
CGGCAAGCAGGCGGCGTTGATGGCGCCGACCGAAATTCTCGCGCGCCAGCACATCAAGACCATCGCGCCGCTGGCCGAAC
GCGCCGGCATGCAGGTCGCGATCCTCACCGGCCGCGAGAAGGGCAAGGAGCGTCGCGATCTGTTGGCGCGGCTCGAAGCC
GGCGAGATCGACTTTCTGGTCGGCACCCACGCGCTGATCCAGGACGACGTGACCTACAACTCGCTGGCGCTGGCCGTGGT
CGACGAGCAGCATCGCTTCGGCGTCCGCGAGCGGCTGGCACTGACCTCGAAGGGCGACGCCGTCGACGTGCTGGTGCTGA
GCGCGACGCCGATCCCGCGCACGCTGGTGCTGACCTATTTCGGCGACATGGACGTCTCGGAGTTGCGCGAGAAGCCCGCC
GGCCGCCAGCCGATCGACACCCGCGCTTTGTCCGATACCCGCCTGCCCGAAGTGATCGACGGCATCGGCCGCGCCATCGC
CGCCGGCAAGCGCGTCTACTGGATCTGCCCGCTGGTCGAGGAATCCGAAAACGTCAAGCTGACTGACGCCGAGCAGCGCT
ACGAGTCGCTGCGGCAGCGCTTCGGCGATCAGCAGGTCGGTCTGGTGCACGGCCGGATGAAGGGCGCCGACAAAGACCGC
GTGATGGCGCAGTTCGCCGGCGGCGAGATCAGCGTGCTGGTCGCCACCACCGTCGTGGAAGTCGGCGTCGACGTGCCCGA
GGCCAGTATCATGGTGATCGAGAACGCCGAGCGCTTCGGCCTCGCGCAACTGCATCAATTGCGCGGTCGCATCGGTCGCG
GCAGCGAGGCCTCGACCTGCCTGCTGCTGTACAAGGAGCCGCTCGGTGAGATGTCGGCGGCGCGGCTGCGGGTGATCCGC
GAAACCGCCGATGGCTTCCGCATTGCCGAAGAAGATCTCAAGCTGCGCGGCGAAGGCGACGTGCTCGGCACCCGCCAGAG
CGGCCTGCCCGGCTACCGCATCGCGCGCTCCGAGGTGCACGGCCAGTTGATCACCCAGGCCCGCGACGAAGCGCTGCGCA
TCCTCGAGGACAATCCCAAGCTCGAAGGCCCATCCGGCGAGGCGCTGCGCTGCCTGCTGTATCTCTACGAACGCGACGAG
GCGATTCCGCTGCTCGGCGCGGGGTAG

Upstream 100 bases:

>100_bases
CGCCGCTCGATCGTGTCGTACCGGTCATCGTTGAAAACCTTGCTGAACGCGGAAAGCCCGGACGTGCCGGGCGAGCCTGA
TATAGCCCCCGGGATCGGCA

Downstream 100 bases:

>100_bases
TGACATCGCGTGATGGTCACCTCGTCTTCACCGCCATGTTCAACGCCGTCGGGTGGGCAAAGGCGCGCCTTTGCCTACCC
TACGAATTCACTTCACGATC

Product: DEAD/DEAH box helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MSARRGALQLAQNASKAAGHIRPNRAGPMRPALLNPLFAPVTGLTGVGPKQDKLFRYLLDRDDTPRLADLLLHLPVSVID
RRSRPKIRDAQPGTVVTLEVTVDRHRPPPPGRSRAPYLVYASDETGDVILTFFRAKPEFVEKLLPVGAKRYVSGTGQLYD
GTLQIVHPDRVVDEEGFAKLPKIDPVYPLTEGLAIGSLRRAVAQALTRLPALPEWISPEVLRRCSFPAFAEALQRVHIPH
EPTDILPDGPFWSRLAYDELLAGQLALALVRAQLRRPAGTRNAGDGRLRHKIIDALPYALTASQQAAAAAIAEDLQQPVR
MLRLLQGDVGSGKTVVALLAAAAVAEAGKQAALMAPTEILARQHIKTIAPLAERAGMQVAILTGREKGKERRDLLARLEA
GEIDFLVGTHALIQDDVTYNSLALAVVDEQHRFGVRERLALTSKGDAVDVLVLSATPIPRTLVLTYFGDMDVSELREKPA
GRQPIDTRALSDTRLPEVIDGIGRAIAAGKRVYWICPLVEESENVKLTDAEQRYESLRQRFGDQQVGLVHGRMKGADKDR
VMAQFAGGEISVLVATTVVEVGVDVPEASIMVIENAERFGLAQLHQLRGRIGRGSEASTCLLLYKEPLGEMSAARLRVIR
ETADGFRIAEEDLKLRGEGDVLGTRQSGLPGYRIARSEVHGQLITQARDEALRILEDNPKLEGPSGEALRCLLYLYERDE
AIPLLGAG

Sequences:

>Translated_728_residues
MSARRGALQLAQNASKAAGHIRPNRAGPMRPALLNPLFAPVTGLTGVGPKQDKLFRYLLDRDDTPRLADLLLHLPVSVID
RRSRPKIRDAQPGTVVTLEVTVDRHRPPPPGRSRAPYLVYASDETGDVILTFFRAKPEFVEKLLPVGAKRYVSGTGQLYD
GTLQIVHPDRVVDEEGFAKLPKIDPVYPLTEGLAIGSLRRAVAQALTRLPALPEWISPEVLRRCSFPAFAEALQRVHIPH
EPTDILPDGPFWSRLAYDELLAGQLALALVRAQLRRPAGTRNAGDGRLRHKIIDALPYALTASQQAAAAAIAEDLQQPVR
MLRLLQGDVGSGKTVVALLAAAAVAEAGKQAALMAPTEILARQHIKTIAPLAERAGMQVAILTGREKGKERRDLLARLEA
GEIDFLVGTHALIQDDVTYNSLALAVVDEQHRFGVRERLALTSKGDAVDVLVLSATPIPRTLVLTYFGDMDVSELREKPA
GRQPIDTRALSDTRLPEVIDGIGRAIAAGKRVYWICPLVEESENVKLTDAEQRYESLRQRFGDQQVGLVHGRMKGADKDR
VMAQFAGGEISVLVATTVVEVGVDVPEASIMVIENAERFGLAQLHQLRGRIGRGSEASTCLLLYKEPLGEMSAARLRVIR
ETADGFRIAEEDLKLRGEGDVLGTRQSGLPGYRIARSEVHGQLITQARDEALRILEDNPKLEGPSGEALRCLLYLYERDE
AIPLLGAG
>Mature_727_residues
SARRGALQLAQNASKAAGHIRPNRAGPMRPALLNPLFAPVTGLTGVGPKQDKLFRYLLDRDDTPRLADLLLHLPVSVIDR
RSRPKIRDAQPGTVVTLEVTVDRHRPPPPGRSRAPYLVYASDETGDVILTFFRAKPEFVEKLLPVGAKRYVSGTGQLYDG
TLQIVHPDRVVDEEGFAKLPKIDPVYPLTEGLAIGSLRRAVAQALTRLPALPEWISPEVLRRCSFPAFAEALQRVHIPHE
PTDILPDGPFWSRLAYDELLAGQLALALVRAQLRRPAGTRNAGDGRLRHKIIDALPYALTASQQAAAAAIAEDLQQPVRM
LRLLQGDVGSGKTVVALLAAAAVAEAGKQAALMAPTEILARQHIKTIAPLAERAGMQVAILTGREKGKERRDLLARLEAG
EIDFLVGTHALIQDDVTYNSLALAVVDEQHRFGVRERLALTSKGDAVDVLVLSATPIPRTLVLTYFGDMDVSELREKPAG
RQPIDTRALSDTRLPEVIDGIGRAIAAGKRVYWICPLVEESENVKLTDAEQRYESLRQRFGDQQVGLVHGRMKGADKDRV
MAQFAGGEISVLVATTVVEVGVDVPEASIMVIENAERFGLAQLHQLRGRIGRGSEASTCLLLYKEPLGEMSAARLRVIRE
TADGFRIAEEDLKLRGEGDVLGTRQSGLPGYRIARSEVHGQLITQARDEALRILEDNPKLEGPSGEALRCLLYLYERDEA
IPLLGAG

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=655, Percent_Identity=42.2900763358779, Blast_Score=437, Evalue=1e-123,
Organism=Escherichia coli, GI1787357, Length=382, Percent_Identity=37.696335078534, Blast_Score=243, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79492; Mature: 79360

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARRGALQLAQNASKAAGHIRPNRAGPMRPALLNPLFAPVTGLTGVGPKQDKLFRYLLD
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHCCCCCCCCCHHHHHHHHHC
RDDTPRLADLLLHLPVSVIDRRSRPKIRDAQPGTVVTLEVTVDRHRPPPPGRSRAPYLVY
CCCCHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEE
ASDETGDVILTFFRAKPEFVEKLLPVGAKRYVSGTGQLYDGTLQIVHPDRVVDEEGFAKL
ECCCCCCEEEEEECCCHHHHHHHHCCCHHHHHCCCCEEECCEEEEECCCHHCCCCCCCCC
PKIDPVYPLTEGLAIGSLRRAVAQALTRLPALPEWISPEVLRRCSFPAFAEALQRVHIPH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCC
EPTDILPDGPFWSRLAYDELLAGQLALALVRAQLRRPAGTRNAGDGRLRHKIIDALPYAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
TASQQAAAAAIAEDLQQPVRMLRLLQGDVGSGKTVVALLAAAAVAEAGKQAALMAPTEIL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHH
ARQHIKTIAPLAERAGMQVAILTGREKGKERRDLLARLEAGEIDFLVGTHALIQDDVTYN
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCC
SLALAVVDEQHRFGVRERLALTSKGDAVDVLVLSATPIPRTLVLTYFGDMDVSELREKPA
EEEEEEECCHHHCCHHHHHEECCCCCEEEEEEEECCCCCCEEEEEEECCCCHHHHHHCCC
GRQPIDTRALSDTRLPEVIDGIGRAIAAGKRVYWICPLVEESENVKLTDAEQRYESLRQR
CCCCCCHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEECCHHHHHHHHHHH
FGDQQVGLVHGRMKGADKDRVMAQFAGGEISVLVATTVVEVGVDVPEASIMVIENAERFG
HCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCEEEEEECCCHHH
LAQLHQLRGRIGRGSEASTCLLLYKEPLGEMSAARLRVIRETADGFRIAEEDLKLRGEGD
HHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHHEECCCCC
VLGTRQSGLPGYRIARSEVHGQLITQARDEALRILEDNPKLEGPSGEALRCLLYLYERDE
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
AIPLLGAG
CCCCCCCC
>Mature Secondary Structure 
SARRGALQLAQNASKAAGHIRPNRAGPMRPALLNPLFAPVTGLTGVGPKQDKLFRYLLD
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHCCCCCCCCCHHHHHHHHHC
RDDTPRLADLLLHLPVSVIDRRSRPKIRDAQPGTVVTLEVTVDRHRPPPPGRSRAPYLVY
CCCCHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEE
ASDETGDVILTFFRAKPEFVEKLLPVGAKRYVSGTGQLYDGTLQIVHPDRVVDEEGFAKL
ECCCCCCEEEEEECCCHHHHHHHHCCCHHHHHCCCCEEECCEEEEECCCHHCCCCCCCCC
PKIDPVYPLTEGLAIGSLRRAVAQALTRLPALPEWISPEVLRRCSFPAFAEALQRVHIPH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCC
EPTDILPDGPFWSRLAYDELLAGQLALALVRAQLRRPAGTRNAGDGRLRHKIIDALPYAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
TASQQAAAAAIAEDLQQPVRMLRLLQGDVGSGKTVVALLAAAAVAEAGKQAALMAPTEIL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHH
ARQHIKTIAPLAERAGMQVAILTGREKGKERRDLLARLEAGEIDFLVGTHALIQDDVTYN
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCC
SLALAVVDEQHRFGVRERLALTSKGDAVDVLVLSATPIPRTLVLTYFGDMDVSELREKPA
EEEEEEECCHHHCCHHHHHEECCCCCEEEEEEEECCCCCCEEEEEEECCCCHHHHHHCCC
GRQPIDTRALSDTRLPEVIDGIGRAIAAGKRVYWICPLVEESENVKLTDAEQRYESLRQR
CCCCCCHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEECCHHHHHHHHHHH
FGDQQVGLVHGRMKGADKDRVMAQFAGGEISVLVATTVVEVGVDVPEASIMVIENAERFG
HCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCEEEEEECCCHHH
LAQLHQLRGRIGRGSEASTCLLLYKEPLGEMSAARLRVIRETADGFRIAEEDLKLRGEGD
HHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHHEECCCCC
VLGTRQSGLPGYRIARSEVHGQLITQARDEALRILEDNPKLEGPSGEALRCLLYLYERDE
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
AIPLLGAG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]