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The map label for this gene is queF

Identifier: 86749896

GI number: 86749896

Start: 3167037

End: 3167513

Strand: Direct

Name: queF

Synonym: RPB_2779

Alternate gene names: 86749896

Gene position: 3167037-3167513 (Clockwise)

Preceding gene: 86749892

Following gene: 86749897

Centisome position: 59.4

GC content: 64.57

Gene sequence:

>477_bases
ATGTCCAGATCGCCCCGCAAAACCTCCAAATCAGCCGGCCTGCAACTCGGCCGCGCCGTCGCCTGGCCGGCCACGCCCGA
CGCCGCCCAGATCGACCGCGTCCCCAATCCGCAGGCCGGCACCGATTATCTGGTGCGCTTCACCGCGCCGGAATTCACCT
CGCTGTGTCCGGTCACCGGCCAGCCGGACTTCGCCCATCTGGTGATCGACTACGCGCCGGGCGCCTGGCTGGTGGAATCG
AAATCGCTGAAGCTGTATCTCGCCAGCTTCCGCAATCACGGCGGCTTCCACGAGGACTGCACGGTGTCGATCGGCAAGCG
GATCGCTGCCGAGATCAAGCCGAAATGGCTGCGCATCGGCGGCTATTGGTATCCGCGCGGAGGAATTCCGATCGATGTGT
TCTGGCAGACCGGGAAACTGCCGAAAAACGTCTGGGTGCCGGATCAGGGCGTCGCAACCTATCGCGGGCGTGGATAA

Upstream 100 bases:

>100_bases
GATTGCGCCGCTTCTACAGCATGGCTTCGGGGCGGAAAAGGCCGTCGCGGAGATGACGGGCGGGTGACGAAAGGCTAAGA
CGGCGGCAACGGAGACCCCC

Downstream 100 bases:

>100_bases
TCGCGCGGCCGCACATCTTCGCAGTTGCGAGCAAAGGGAACTTCACGACGTCGGGAACGTTGACTTGGAACTTTCTCGAC
TCTTGGAGCGTCCCATTGAA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSRSPRKTSKSAGLQLGRAVAWPATPDAAQIDRVPNPQAGTDYLVRFTAPEFTSLCPVTGQPDFAHLVIDYAPGAWLVES
KSLKLYLASFRNHGGFHEDCTVSIGKRIAAEIKPKWLRIGGYWYPRGGIPIDVFWQTGKLPKNVWVPDQGVATYRGRG

Sequences:

>Translated_158_residues
MSRSPRKTSKSAGLQLGRAVAWPATPDAAQIDRVPNPQAGTDYLVRFTAPEFTSLCPVTGQPDFAHLVIDYAPGAWLVES
KSLKLYLASFRNHGGFHEDCTVSIGKRIAAEIKPKWLRIGGYWYPRGGIPIDVFWQTGKLPKNVWVPDQGVATYRGRG
>Mature_157_residues
SRSPRKTSKSAGLQLGRAVAWPATPDAAQIDRVPNPQAGTDYLVRFTAPEFTSLCPVTGQPDFAHLVIDYAPGAWLVESK
SLKLYLASFRNHGGFHEDCTVSIGKRIAAEIKPKWLRIGGYWYPRGGIPIDVFWQTGKLPKNVWVPDQGVATYRGRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_RHOP2 (Q2IWC9)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486392.1
- ProteinModelPortal:   Q2IWC9
- SMR:   Q2IWC9
- STRING:   Q2IWC9
- GeneID:   3910572
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2779
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   HEDCTVA
- ProtClustDB:   PRK13258
- BioCyc:   RPAL316058:RPB_2779-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 17439; Mature: 17308

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSPRKTSKSAGLQLGRAVAWPATPDAAQIDRVPNPQAGTDYLVRFTAPEFTSLCPVTG
CCCCCCCCCCCCCCCCCCEEECCCCCCHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCC
QPDFAHLVIDYAPGAWLVESKSLKLYLASFRNHGGFHEDCTVSIGKRIAAEIKPKWLRIG
CCCEEEEEEEECCCEEEEECCCEEEEEEEHHCCCCCCCCCEEHHHHHHHHHCCCEEEEEC
GYWYPRGGIPIDVFWQTGKLPKNVWVPDQGVATYRGRG
CEEECCCCCEEEEEEECCCCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure 
SRSPRKTSKSAGLQLGRAVAWPATPDAAQIDRVPNPQAGTDYLVRFTAPEFTSLCPVTG
CCCCCCCCCCCCCCCCCEEECCCCCCHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCC
QPDFAHLVIDYAPGAWLVESKSLKLYLASFRNHGGFHEDCTVSIGKRIAAEIKPKWLRIG
CCCEEEEEEEECCCEEEEECCCEEEEEEEHHCCCCCCCCCEEHHHHHHHHHCCCEEEEEC
GYWYPRGGIPIDVFWQTGKLPKNVWVPDQGVATYRGRG
CEEECCCCCEEEEEEECCCCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA