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The map label for this gene is rhtB [H]

Identifier: 86749892

GI number: 86749892

Start: 3162758

End: 3163375

Strand: Direct

Name: rhtB [H]

Synonym: RPB_2775

Alternate gene names: 86749892

Gene position: 3162758-3163375 (Clockwise)

Preceding gene: 86749880

Following gene: 86749896

Centisome position: 59.32

GC content: 66.34

Gene sequence:

>618_bases
ATGACGATCAGCGCCGAATTCCTCATCACCTCGTTGATCGTGGTGGCCTCGCCCGGCACGGGCGCGCTGTACACGATCGC
GGCCGGGCTTTCGCGCGGATCGCGGGCCAGCCTGATCGCCGCCTTCGGCTCGACGCTCGGGATCGTCCCGCACATGGCGG
CGGCGATCGTGGGGCTCGCCGCCCTGCTCCACACCAGCGCGGTGGCGTTCCAGACCTTCAAATATCTCGGCGTGGCCTAT
CTGCTCTACATGGCGTGGAAGACGCTCGGCGAGCGCGGCCCGCTGAGTGTCGAGGCGGAGGTCGGGGCGCGCTCGGCGCT
GCAGATGACCGTGACCGGCGTGCTGATCAACATCCTCAATCCGAAGTTGTCGATCTTCTTCCTCGCCTTCCTGCCGCAGT
TCGTCTCGGCCGACGACGCACATCCGCTCGGCCGCATGGTCGAACTGAGTGCCATCTTCATGCTGATGACATTCGTCGTA
TTCGCGATCTACGGCCTGTTCGCTTCTTGGCTGCGCGACCACGTCGTGACGCGGCCTAAAGTGCTGACCTGGATGCGCCG
CAGCTTCGCGGCCGCCTTCGTCGCCCTCGGCGCCAAGCTGGCGACCGCGGAGCGCTGA

Upstream 100 bases:

>100_bases
CATCTGCTGCTCCGTTTCGATAGACATGCCGGGGTTGGCGCGGCACTGTTTCCGTGCCGAGACGCAAATTGCAAGTCCAT
CTCAACAGTGGGACCCTGCC

Downstream 100 bases:

>100_bases
GGCGTCAGGTCCGCGCGCGGATTTCGTTGAGCAGGAAGTTGCCGATACGCTTGCCGCGATTGCGCAGCACCTTAACGACG
CCCTGATCGACGAAATAGGT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTISAEFLITSLIVVASPGTGALYTIAAGLSRGSRASLIAAFGSTLGIVPHMAAAIVGLAALLHTSAVAFQTFKYLGVAY
LLYMAWKTLGERGPLSVEAEVGARSALQMTVTGVLINILNPKLSIFFLAFLPQFVSADDAHPLGRMVELSAIFMLMTFVV
FAIYGLFASWLRDHVVTRPKVLTWMRRSFAAAFVALGAKLATAER

Sequences:

>Translated_205_residues
MTISAEFLITSLIVVASPGTGALYTIAAGLSRGSRASLIAAFGSTLGIVPHMAAAIVGLAALLHTSAVAFQTFKYLGVAY
LLYMAWKTLGERGPLSVEAEVGARSALQMTVTGVLINILNPKLSIFFLAFLPQFVSADDAHPLGRMVELSAIFMLMTFVV
FAIYGLFASWLRDHVVTRPKVLTWMRRSFAAAFVALGAKLATAER
>Mature_204_residues
TISAEFLITSLIVVASPGTGALYTIAAGLSRGSRASLIAAFGSTLGIVPHMAAAIVGLAALLHTSAVAFQTFKYLGVAYL
LYMAWKTLGERGPLSVEAEVGARSALQMTVTGVLINILNPKLSIFFLAFLPQFVSADDAHPLGRMVELSAIFMLMTFVVF
AIYGLFASWLRDHVVTRPKVLTWMRRSFAAAFVALGAKLATAER

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=203, Percent_Identity=27.0935960591133, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788099, Length=206, Percent_Identity=26.6990291262136, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21900; Mature: 21769

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISAEFLITSLIVVASPGTGALYTIAAGLSRGSRASLIAAFGSTLGIVPHMAAAIVGLA
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLHTSAVAFQTFKYLGVAYLLYMAWKTLGERGPLSVEAEVGARSALQMTVTGVLINILN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHC
PKLSIFFLAFLPQFVSADDAHPLGRMVELSAIFMLMTFVVFAIYGLFASWLRDHVVTRPK
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VLTWMRRSFAAAFVALGAKLATAER
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TISAEFLITSLIVVASPGTGALYTIAAGLSRGSRASLIAAFGSTLGIVPHMAAAIVGLA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLHTSAVAFQTFKYLGVAYLLYMAWKTLGERGPLSVEAEVGARSALQMTVTGVLINILN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHC
PKLSIFFLAFLPQFVSADDAHPLGRMVELSAIFMLMTFVVFAIYGLFASWLRDHVVTRPK
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VLTWMRRSFAAAFVALGAKLATAER
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]