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The map label for this gene is malF [C]

Identifier: 86749666

GI number: 86749666

Start: 2922194

End: 2923132

Strand: Direct

Name: malF [C]

Synonym: RPB_2547

Alternate gene names: 86749666

Gene position: 2922194-2923132 (Clockwise)

Preceding gene: 86749665

Following gene: 86749667

Centisome position: 54.81

GC content: 62.94

Gene sequence:

>939_bases
ATGGTCGACATTCCGTATCAGCCGGGCAGGGTCGCCGCGGCGTCCACCCGCCGCAGGCGGGGCGGGCTCGCGGCGATGAT
GAAGCGCAAATCGATGGTGGCGTTCCTGATGGCGCTGCCGCTGATCGTGCTGATCGGCGTGCTGGTGATCTATCCGGCGT
TCTACGCGGTGCATCTGGCGACGCTGAACAAGTCGATGCAGCGCTTCGTCGGCTTCGACAATTTTCTGTTCCTGTTCAAG
CGCGACACGTTCTGGTTGGTGGTGCAGCAATCCTGCATTTTCGCGATCACCGCCGTGGTGTTCAAGGCGCTGCTCGGCTT
CATCGTCGCGCATTTCGTCCACAACGTGCCGGCCAAGGGCCAGCGCAAATGGCGCGGCATGCTGCTGGTGCCGTGGGTGA
TCCCGCCGGCGATGTCGACGCTGGCCTGGCTGTGGCTGTTCGACCCGTCCTACAGCGCGTTCAACTACACGCTCGGCCTG
TTCGGCGCCGGCCCGATTCCCTGGACCGGCGACGCGTCCTGGGCGCGGTTCTCGGTCATTCTGGTCAATATCTGGTATGG
CGCGCCGTTCTTTATGATCATGTATCTGGCGGCGCTGAAATCGGTGCCGGAGCAGCTCTACGAGGCGGCGTCGATCGACG
GCGCCAATTGGTGGCAGAAGCTGTGGTACGTGACGCTGCCGATGATGCGCAACATCATCGCCATCACCACGCTGTTCTCG
CTGATCGTCACTTTCGCCAATTTCGACATCGTCCGCATCCTCACCGCCGGCGGCCCGCTCGACCACACCCATATCTTCGC
CACCTGGGCGTTCCGGATCGGCATCGAGGGCTCGGACATTCCGCTCGGCGCCAGCGTGTCGCTGTTCATGCTGCCGATCC
TCGGCGTCGCGGCGATCTTCATCTTGCGCGACATCAACAAACGCGGGAACGAAAGCTGA

Upstream 100 bases:

>100_bases
GTCATTGCGAGCCGACGGCGAAGCAATCCAGAGCACCGACCGCTGCGCTGTTGGATTGCTTCGTCGCTTCGCTCCTCGCA
ATGACGGGGAGGAGCCTTCG

Downstream 100 bases:

>100_bases
TGGCCTCGACACTGACGATCGACAAGGCCGCGCCACGGCGCAATCCGGGCTACGGCAGCATGAGCCGCGACCGCGCCTGG
GCGCTGAAATGGTCGTATTT

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MVDIPYQPGRVAAASTRRRRGGLAAMMKRKSMVAFLMALPLIVLIGVLVIYPAFYAVHLATLNKSMQRFVGFDNFLFLFK
RDTFWLVVQQSCIFAITAVVFKALLGFIVAHFVHNVPAKGQRKWRGMLLVPWVIPPAMSTLAWLWLFDPSYSAFNYTLGL
FGAGPIPWTGDASWARFSVILVNIWYGAPFFMIMYLAALKSVPEQLYEAASIDGANWWQKLWYVTLPMMRNIIAITTLFS
LIVTFANFDIVRILTAGGPLDHTHIFATWAFRIGIEGSDIPLGASVSLFMLPILGVAAIFILRDINKRGNES

Sequences:

>Translated_312_residues
MVDIPYQPGRVAAASTRRRRGGLAAMMKRKSMVAFLMALPLIVLIGVLVIYPAFYAVHLATLNKSMQRFVGFDNFLFLFK
RDTFWLVVQQSCIFAITAVVFKALLGFIVAHFVHNVPAKGQRKWRGMLLVPWVIPPAMSTLAWLWLFDPSYSAFNYTLGL
FGAGPIPWTGDASWARFSVILVNIWYGAPFFMIMYLAALKSVPEQLYEAASIDGANWWQKLWYVTLPMMRNIIAITTLFS
LIVTFANFDIVRILTAGGPLDHTHIFATWAFRIGIEGSDIPLGASVSLFMLPILGVAAIFILRDINKRGNES
>Mature_312_residues
MVDIPYQPGRVAAASTRRRRGGLAAMMKRKSMVAFLMALPLIVLIGVLVIYPAFYAVHLATLNKSMQRFVGFDNFLFLFK
RDTFWLVVQQSCIFAITAVVFKALLGFIVAHFVHNVPAKGQRKWRGMLLVPWVIPPAMSTLAWLWLFDPSYSAFNYTLGL
FGAGPIPWTGDASWARFSVILVNIWYGAPFFMIMYLAALKSVPEQLYEAASIDGANWWQKLWYVTLPMMRNIIAITTLFS
LIVTFANFDIVRILTAGGPLDHTHIFATWAFRIGIEGSDIPLGASVSLFMLPILGVAAIFILRDINKRGNES

Specific function: Probably part of the binding-protein-dependent transport system y4oPQRS. This system probably transports a sugar-like molecule. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790465, Length=225, Percent_Identity=30.2222222222222, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789861, Length=295, Percent_Identity=25.0847457627119, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787570, Length=255, Percent_Identity=26.2745098039216, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35021; Mature: 35021

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDIPYQPGRVAAASTRRRRGGLAAMMKRKSMVAFLMALPLIVLIGVLVIYPAFYAVHLA
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLNKSMQRFVGFDNFLFLFKRDTFWLVVQQSCIFAITAVVFKALLGFIVAHFVHNVPAKG
HHHHHHHHHHCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QRKWRGMLLVPWVIPPAMSTLAWLWLFDPSYSAFNYTLGLFGAGPIPWTGDASWARFSVI
CHHHCCEEEECCCCCHHHHHHHHHHEECCCCCHHHEEEEECCCCCCCCCCCCCHHHHEEE
LVNIWYGAPFFMIMYLAALKSVPEQLYEAASIDGANWWQKLWYVTLPMMRNIIAITTLFS
EEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVTFANFDIVRILTAGGPLDHTHIFATWAFRIGIEGSDIPLGASVSLFMLPILGVAAIF
HHHHHHCCCEEEEEECCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
ILRDINKRGNES
HHHHHHHCCCCC
>Mature Secondary Structure
MVDIPYQPGRVAAASTRRRRGGLAAMMKRKSMVAFLMALPLIVLIGVLVIYPAFYAVHLA
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLNKSMQRFVGFDNFLFLFKRDTFWLVVQQSCIFAITAVVFKALLGFIVAHFVHNVPAKG
HHHHHHHHHHCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QRKWRGMLLVPWVIPPAMSTLAWLWLFDPSYSAFNYTLGLFGAGPIPWTGDASWARFSVI
CHHHCCEEEECCCCCHHHHHHHHHHEECCCCCHHHEEEEECCCCCCCCCCCCCHHHHEEE
LVNIWYGAPFFMIMYLAALKSVPEQLYEAASIDGANWWQKLWYVTLPMMRNIIAITTLFS
EEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVTFANFDIVRILTAGGPLDHTHIFATWAFRIGIEGSDIPLGASVSLFMLPILGVAAIF
HHHHHHCCCEEEEEECCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
ILRDINKRGNES
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]