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The map label for this gene is smf [H]

Identifier: 86749541

GI number: 86749541

Start: 2775650

End: 2776786

Strand: Reverse

Name: smf [H]

Synonym: RPB_2421

Alternate gene names: 86749541

Gene position: 2776786-2775650 (Counterclockwise)

Preceding gene: 86749542

Following gene: 86749539

Centisome position: 52.08

GC content: 72.03

Gene sequence:

>1137_bases
TTGGGCGAGCGTAGCAGTGATCAGGGGACGACCGTTCTCACCGAGGCCCAGCGGGTCGACTGGCTGCGGCTGATCCGGGC
CGACAATGTCGGGCCGCGCACCTTCCGCTCGCTGATCAATCATTTCGGCTCGGCGCGCGCGGCGCTGGAGCGGTTGCCGG
AACTGGCGCGGCGCGGCGGCGCGGCGCGCGCCGGACGTATCCCGTCCGAAGATGAGGCGCGGCGCGAGATCGAGGGCGGG
CGGCGGCTCGGTGTCGAGCTGGTGGCGCCGGGCGAGCCTGGCTACCCGCCTCGCCTCGCGACGATCGACGATGCGCCGCC
GCTGCTCGGCGTCCATGCGCTGCCCGACGCGCTGGCGCTGATGCAGCGGCCGATGATCGCGATCGTCGGCTCACGCAACG
CTTCCGGCGCCGGGTTGAAGTTCGCCGGCCTGCTCGCCGGCGACCTCGGCGCGAGCGGCTTCGTGGTGATCTCCGGGCTG
GCGCGCGGCATCGACCAGGCGGCGCATCGCGCCAGTCTCGGCAGCGGCACCGTCGCGGTGCTGGCCGGCGGCCATGACAA
GATCTATCCGGCAGAGCACGAGGATCTGCTGCTCGACATTATCGAAGCGCGCGGCGCGGCGATCTCGGAAATGCCACTCG
GCCATGTCCCACGCGGCAAGGATTTTCCCCGCCGCAACCGGCTGATCTCCGGCGCCGCGGTCGGCGTGGTGGTGATCGAG
GCGGCGTATCGCTCCGGCTCGCTGATCACCGCGCGGCGCGCCGCCGATCAGGGCCGCGAGGTGTTCGCGGTGCCGGGCTC
GCCACTCGATCCGCGCGCCGCCGGCACCAACGATCTGATCAAGCAGGGCGCGACGCTGATCACCTGCGCCGCCGACATCA
TCCAGGCGGTGGCGCCGATCCTCGATCGGCCGATCGAGCTGCCCGGCCGCGAACCCGACCACCCGCCGCCGGCAAGCGAT
CCAGATCCCAGCGACCGCAGCCGGATCGTCAACCTGCTGGGGCCGAGCCCTGTCGGAATCGACGATCTGATCCGGCTGAG
CGGTATCGCTCCCGCGGTGGTGCGCACCGTGCTGCTGGAGCTGGAACTCGCCGGCCGGCTGGAGCGCCATGGCGGCGGGC
TGGTGTCGCTGCTGTGA

Upstream 100 bases:

>100_bases
TTCAAGAACGCAGTGGCTCTCTCCGCACCTATGACGTCCACCCCTCGCCAGTCTTGATCACACGCATCAGTCGCGCGACA
CTCCCGGCCGGGGGTGCACT

Downstream 100 bases:

>100_bases
GACCGGGCTGCGCCACAAAAAGCAACGGCCCGAAGTGGTGACACTTCGAGCCATCTCGGAGGCGGACGTACATCCGGCCC
GCCTCCCGCTTATTTCGAAT

Product: DNA processing protein DprA, putative

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MGERSSDQGTTVLTEAQRVDWLRLIRADNVGPRTFRSLINHFGSARAALERLPELARRGGAARAGRIPSEDEARREIEGG
RRLGVELVAPGEPGYPPRLATIDDAPPLLGVHALPDALALMQRPMIAIVGSRNASGAGLKFAGLLAGDLGASGFVVISGL
ARGIDQAAHRASLGSGTVAVLAGGHDKIYPAEHEDLLLDIIEARGAAISEMPLGHVPRGKDFPRRNRLISGAAVGVVVIE
AAYRSGSLITARRAADQGREVFAVPGSPLDPRAAGTNDLIKQGATLITCAADIIQAVAPILDRPIELPGREPDHPPPASD
PDPSDRSRIVNLLGPSPVGIDDLIRLSGIAPAVVRTVLLELELAGRLERHGGGLVSLL

Sequences:

>Translated_378_residues
MGERSSDQGTTVLTEAQRVDWLRLIRADNVGPRTFRSLINHFGSARAALERLPELARRGGAARAGRIPSEDEARREIEGG
RRLGVELVAPGEPGYPPRLATIDDAPPLLGVHALPDALALMQRPMIAIVGSRNASGAGLKFAGLLAGDLGASGFVVISGL
ARGIDQAAHRASLGSGTVAVLAGGHDKIYPAEHEDLLLDIIEARGAAISEMPLGHVPRGKDFPRRNRLISGAAVGVVVIE
AAYRSGSLITARRAADQGREVFAVPGSPLDPRAAGTNDLIKQGATLITCAADIIQAVAPILDRPIELPGREPDHPPPASD
PDPSDRSRIVNLLGPSPVGIDDLIRLSGIAPAVVRTVLLELELAGRLERHGGGLVSLL
>Mature_377_residues
GERSSDQGTTVLTEAQRVDWLRLIRADNVGPRTFRSLINHFGSARAALERLPELARRGGAARAGRIPSEDEARREIEGGR
RLGVELVAPGEPGYPPRLATIDDAPPLLGVHALPDALALMQRPMIAIVGSRNASGAGLKFAGLLAGDLGASGFVVISGLA
RGIDQAAHRASLGSGTVAVLAGGHDKIYPAEHEDLLLDIIEARGAAISEMPLGHVPRGKDFPRRNRLISGAAVGVVVIEA
AYRSGSLITARRAADQGREVFAVPGSPLDPRAAGTNDLIKQGATLITCAADIIQAVAPILDRPIELPGREPDHPPPASDP
DPSDRSRIVNLLGPSPVGIDDLIRLSGIAPAVVRTVLLELELAGRLERHGGGLVSLL

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=290, Percent_Identity=40.3448275862069, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 39551; Mature: 39420

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGERSSDQGTTVLTEAQRVDWLRLIRADNVGPRTFRSLINHFGSARAALERLPELARRGG
CCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AARAGRIPSEDEARREIEGGRRLGVELVAPGEPGYPPRLATIDDAPPLLGVHALPDALAL
CCCCCCCCCHHHHHHHHCCCCEECEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MQRPMIAIVGSRNASGAGLKFAGLLAGDLGASGFVVISGLARGIDQAAHRASLGSGTVAV
HHCCEEEEEECCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LAGGHDKIYPAEHEDLLLDIIEARGAAISEMPLGHVPRGKDFPRRNRLISGAAVGVVVIE
EECCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHEEE
AAYRSGSLITARRAADQGREVFAVPGSPLDPRAAGTNDLIKQGATLITCAADIIQAVAPI
EHHCCCCEEEEHHHHCCCCEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
LDRPIELPGREPDHPPPASDPDPSDRSRIVNLLGPSPVGIDDLIRLSGIAPAVVRTVLLE
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHH
LELAGRLERHGGGLVSLL
HHHHHHHHHCCCCEEECC
>Mature Secondary Structure 
GERSSDQGTTVLTEAQRVDWLRLIRADNVGPRTFRSLINHFGSARAALERLPELARRGG
CCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AARAGRIPSEDEARREIEGGRRLGVELVAPGEPGYPPRLATIDDAPPLLGVHALPDALAL
CCCCCCCCCHHHHHHHHCCCCEECEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MQRPMIAIVGSRNASGAGLKFAGLLAGDLGASGFVVISGLARGIDQAAHRASLGSGTVAV
HHCCEEEEEECCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LAGGHDKIYPAEHEDLLLDIIEARGAAISEMPLGHVPRGKDFPRRNRLISGAAVGVVVIE
EECCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHEEE
AAYRSGSLITARRAADQGREVFAVPGSPLDPRAAGTNDLIKQGATLITCAADIIQAVAPI
EHHCCCCEEEEHHHHCCCCEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
LDRPIELPGREPDHPPPASDPDPSDRSRIVNLLGPSPVGIDDLIRLSGIAPAVVRTVLLE
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHH
LELAGRLERHGGGLVSLL
HHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]