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The map label for this gene is pcaR [H]

Identifier: 86749387

GI number: 86749387

Start: 2616622

End: 2617488

Strand: Reverse

Name: pcaR [H]

Synonym: RPB_2267

Alternate gene names: 86749387

Gene position: 2617488-2616622 (Counterclockwise)

Preceding gene: 86749409

Following gene: 86749385

Centisome position: 49.09

GC content: 63.21

Gene sequence:

>867_bases
ATGAACAATCCGTCTGACGCCCTTGTGTTCGCACAACGAACACGCTTAGGATCACCAACCCGCTGTCTTCAATGTCCCCA
GATGCCGAAACTGAAACGCGACGAGAGCGAACTGCCGGATACCGAGCACATCGAAAGCCTGGCGCGCGGCCTTCGGCTGA
TCGAGGTGTTCGGGGCCGAACGGCGCCCCATGACGTTAAGCGATGTCGCGAAGGCTTGCGGCCTACCGCGCGCCACGGCG
CGGCGCATTCTTCTCACGCTGCAGAAATCCGGCTTCGTCACCAGCGACGAGCGGCTGTTCGAATTGACCCCGCGCGTACT
GCGGCTTGCATCGGCGTATCTGGCTTCGAACCAGATCAGCACCCTGCTGCAGCCGGCGATGGATCTGGTCTCGGACAAGG
CCAAGGAGGTCTGCTCGCTGGCGATCTGGGATGGGGACGACGCGGTGTTCGTCGCACGGTCGAGTCCGGCGCGGGTGTTT
TCGGCCGGCATCGATCTCGGCTATCGGCTGCCGCTGTTCTGCACGTCCGTCGGCCGGGTCCTGCTCGGCCGGCTCGGCAA
TGACGACCTCGCCGCGACGATCGACCGGATGACGCTGACGAAGCAGACCGACACGACCCTGATCGACAAGCCGACATTGG
TGGCGACCATCATCGCCGATCGGACCAAGGGATATTCACTGGTCGATTGCGAGGCCGAGCCGGCATTCCGCTCGATCGCC
GTGCCTGTTCGCCGCTACGATGGAATGATCGTCGCCGCCGCCAATATCGGCGCCCATGTGGACCGGATCACGACCGGCGA
AATGATCGACCGGTTTCTGCCGCCGCTGAAACGCATGGCCGAAGATGTCCGGCCTTTGGTGATGTGA

Upstream 100 bases:

>100_bases
GAATAAGAATGCCTCTTAGGAGCGAAGCCTGCAAGCCCATTTTGTGTCCCAAAACTGCCCAACAGAGGTGCAGGCAAGTT
CCGGATACCGATGTGCATTA

Downstream 100 bases:

>100_bases
CTTCGAGCCAAAGCGGCGGCCGGGGCGCGAAGCGGAACTCGCGAGCGCGACAGAACGGACCTCGCACCAACCCGTCGCAG
CGCGGTTGGACCTGTTAACC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MNNPSDALVFAQRTRLGSPTRCLQCPQMPKLKRDESELPDTEHIESLARGLRLIEVFGAERRPMTLSDVAKACGLPRATA
RRILLTLQKSGFVTSDERLFELTPRVLRLASAYLASNQISTLLQPAMDLVSDKAKEVCSLAIWDGDDAVFVARSSPARVF
SAGIDLGYRLPLFCTSVGRVLLGRLGNDDLAATIDRMTLTKQTDTTLIDKPTLVATIIADRTKGYSLVDCEAEPAFRSIA
VPVRRYDGMIVAAANIGAHVDRITTGEMIDRFLPPLKRMAEDVRPLVM

Sequences:

>Translated_288_residues
MNNPSDALVFAQRTRLGSPTRCLQCPQMPKLKRDESELPDTEHIESLARGLRLIEVFGAERRPMTLSDVAKACGLPRATA
RRILLTLQKSGFVTSDERLFELTPRVLRLASAYLASNQISTLLQPAMDLVSDKAKEVCSLAIWDGDDAVFVARSSPARVF
SAGIDLGYRLPLFCTSVGRVLLGRLGNDDLAATIDRMTLTKQTDTTLIDKPTLVATIIADRTKGYSLVDCEAEPAFRSIA
VPVRRYDGMIVAAANIGAHVDRITTGEMIDRFLPPLKRMAEDVRPLVM
>Mature_288_residues
MNNPSDALVFAQRTRLGSPTRCLQCPQMPKLKRDESELPDTEHIESLARGLRLIEVFGAERRPMTLSDVAKACGLPRATA
RRILLTLQKSGFVTSDERLFELTPRVLRLASAYLASNQISTLLQPAMDLVSDKAKEVCSLAIWDGDDAVFVARSSPARVF
SAGIDLGYRLPLFCTSVGRVLLGRLGNDDLAATIDRMTLTKQTDTTLIDKPTLVATIIADRTKGYSLVDCEAEPAFRSIA
VPVRRYDGMIVAAANIGAHVDRITTGEMIDRFLPPLKRMAEDVRPLVM

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=229, Percent_Identity=24.8908296943231, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082359, Length=230, Percent_Identity=25.2173913043478, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786715, Length=231, Percent_Identity=27.7056277056277, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789999, Length=246, Percent_Identity=23.5772357723577, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788131, Length=211, Percent_Identity=23.696682464455, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693096, Length=229, Percent_Identity=28.82096069869, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31672; Mature: 31672

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPSDALVFAQRTRLGSPTRCLQCPQMPKLKRDESELPDTEHIESLARGLRLIEVFGAE
CCCCCHHEEEEHHHCCCCCHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCC
RRPMTLSDVAKACGLPRATARRILLTLQKSGFVTSDERLFELTPRVLRLASAYLASNQIS
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLLQPAMDLVSDKAKEVCSLAIWDGDDAVFVARSSPARVFSAGIDLGYRLPLFCTSVGRV
HHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHH
LLGRLGNDDLAATIDRMTLTKQTDTTLIDKPTLVATIIADRTKGYSLVDCEAEPAFRSIA
HHHHCCCCHHHHHHHHHEEECCCCCEECCCCHHEEHEEHHCCCCCEEEECCCCCCHHHHH
VPVRRYDGMIVAAANIGAHVDRITTGEMIDRFLPPLKRMAEDVRPLVM
HHHHHHCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNPSDALVFAQRTRLGSPTRCLQCPQMPKLKRDESELPDTEHIESLARGLRLIEVFGAE
CCCCCHHEEEEHHHCCCCCHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCC
RRPMTLSDVAKACGLPRATARRILLTLQKSGFVTSDERLFELTPRVLRLASAYLASNQIS
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLLQPAMDLVSDKAKEVCSLAIWDGDDAVFVARSSPARVFSAGIDLGYRLPLFCTSVGRV
HHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHH
LLGRLGNDDLAATIDRMTLTKQTDTTLIDKPTLVATIIADRTKGYSLVDCEAEPAFRSIA
HHHHCCCCHHHHHHHHHEEECCCCCEECCCCHHEEHEEHHCCCCCEEEECCCCCCHHHHH
VPVRRYDGMIVAAANIGAHVDRITTGEMIDRFLPPLKRMAEDVRPLVM
HHHHHHCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]