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The map label for this gene is hyfR [H]

Identifier: 86749175

GI number: 86749175

Start: 2333748

End: 2335742

Strand: Reverse

Name: hyfR [H]

Synonym: RPB_2053

Alternate gene names: 86749175

Gene position: 2335742-2333748 (Counterclockwise)

Preceding gene: 86749183

Following gene: 86749168

Centisome position: 43.81

GC content: 67.87

Gene sequence:

>1995_bases
TTGCTACGAAATCTCGTAGTATACGATTTTTCGGAGTCGAATTACGAGATCTCGGAGCAAGTGGTGGATCTGCCGGTACC
CTTTTCCGCAACCGACACCGATCTGCGCGCGGAGGCCTTCGACGGGCTGATCGAGGCGGCGCTGTTGCTCGATCCGGCCG
CCGACCAGATCCTCGAGGTCAATCCCGCGGCCTGCGCCCTGCTCGGCTACGACCGCGCCACGTTGCTGCAGACCCGGATC
AGCGCGCTGCACGACCGGCAATTCCCGGCGCTGATCGTATTCACCCAGGCGGTGTTCGACCGCGGCAGCTATTGGACCCA
CGCGCTGACGCCGAACCATGGCGCCGGCACGCCATTGCGGGTCGAATATGCCGGCCGGGCGCTGCAATCTCGCGGGCGCA
CACTGCTGCTGCTGACGATGAGCGACCTCGAGCAGCGCCGCCGCCGCCATATCGACGCAGCGGCCGACGATTACATGCGC
GACGGACTGCCGGCGTGGCAGCGGGTCGAGCGGGTGTTCGAGGATATCGAGCGCGAGAACCAGTTGATCCTGCGCGCTGC
CGGCGAAGGCATCTACGGCGTCAACGCCGAGGGCCGCGCCACCTTCGTCAACCCGGCGGCGGAACGGATGCTCGGCTGGT
CGGCCGAGGAGCTGGTCGGTCGGTCGATCCACGCCGTGATGCACCACACCCATCACGACGGCCGTCCCTACGCCGACCAC
GACTGCCCGATCTACGCCGCGTTCCGCGACGGCGCGGTGCACACCGTCGACGGCGAAGTGTTCTGGCGCAAGGACGGCAA
GCCGGTGTGGGTCGAGTACACCTCGACGCCGATCCGCGACCGCAGCGGCGTGATCGTCGGCGCCGTCGTGGTGTTTCGCG
ACGTGAGCCAGCGCCGCGAGGCCGACGAGAAGCTGCATGCCGCGCTCGCCGAAGTCGACCGGCTGCGCGAGCGGCTGCAG
CTCGAGAACGATTACTTGCAGGAAGAGATCCGGATCGAGACCAATCCGCGCGGCATCATCGGCCAGAGCGAAGCGATCCA
GACCACGCTGCGCCAGGTCAAGCTGGTGGCGCCGACCACCGCCGCGGTGCTGATCACCGGCGAATCCGGCACCGGCAAGG
AACTGATCGCGCGCGCCATCCACGACGCCAGCACCCGCAGCGGCCGGCCGCTGATCCGGGTCAATTGCGCCGCGATTCCG
CGCGAATTGTTCGAGAGCGAATTCTTCGGCCACACCCGCGGCGCCTTCACCGGGGCGGTGCGCGACCGCATCGGCCGGTT
CGAGCTGGCCGACGGCGGCACGCTGTTCCTCGACGAGATAGGCGAGATCCCGCTGGAGCTGCAGGGCAAGCTGCTGCGCG
TGCTGCAGGAGGGCAATTTCGAGCGGGTCGGCGACGAGCGCACCCGCAATGTCGACGTCCGGCTGATCGCCGCCACCAAT
CGCGACCTGAAGCAGGAGGTGCAGCGCGGCCGTTTCCGCGAGGATCTGTACTTCCGGCTCAACGTGTTTCCGATCGAGTC
GGTGCCGCTACGCGATCGCCGCGAGGATATTCCGTTGCTGGCGCAGCACTTCCTCGCCAGCGAGCGGCGCGAGCTGAAAT
CCGGACTGCGGCTGTCGCAGGGCGACGTGCGGCGGCTGATGCGCTACGAGTGGCCGGGGAACGTCCGCGAATTGCAGAAC
GTGATCGAGCGCGCCACCATCCTGGCACAGAACGGGCGGCTGCGGATCGATTTGCCGGAGCCGTCCGGCCACCATCCCGC
GCCGAACGCCGGCCGGCAGAAATCCGAAACGCGACCCGCGGTGATGACCGCCGCGGAGCTGCGCGATCTCGAGCGCGCCA
ACATCGTCGCCGCGCTACGCGCGTGCAACGGCAAAGTGTTCGGCGACGACGGCGCAGCGGCGATGCTCGACCTCAAGCCG
ACGACGCTGGCGTCGCGGATCAAGGCATTGGGCATCAGCGCGACACGGGCCGCGAACGGCAGTGCAGTCGACTGA

Upstream 100 bases:

>100_bases
TGAGACCTCCTGCGCTGTTTGATCAGATCTAACGCAAGGAGCATGCCAGCTCGGCTTTCGGGACCTTCGCCTTCAAACGG
CGACAGGCTCGCCGACTTGT

Downstream 100 bases:

>100_bases
GATAACACGACCGACCCTTGCCTCGCGCGACAGATCGCCGCTGTTTGCGGCGGAGTACGCCACCGCAACTCCCCAAATTC
ACCGGCCCGGCGGACTCATC

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MLRNLVVYDFSESNYEISEQVVDLPVPFSATDTDLRAEAFDGLIEAALLLDPAADQILEVNPAACALLGYDRATLLQTRI
SALHDRQFPALIVFTQAVFDRGSYWTHALTPNHGAGTPLRVEYAGRALQSRGRTLLLLTMSDLEQRRRRHIDAAADDYMR
DGLPAWQRVERVFEDIERENQLILRAAGEGIYGVNAEGRATFVNPAAERMLGWSAEELVGRSIHAVMHHTHHDGRPYADH
DCPIYAAFRDGAVHTVDGEVFWRKDGKPVWVEYTSTPIRDRSGVIVGAVVVFRDVSQRREADEKLHAALAEVDRLRERLQ
LENDYLQEEIRIETNPRGIIGQSEAIQTTLRQVKLVAPTTAAVLITGESGTGKELIARAIHDASTRSGRPLIRVNCAAIP
RELFESEFFGHTRGAFTGAVRDRIGRFELADGGTLFLDEIGEIPLELQGKLLRVLQEGNFERVGDERTRNVDVRLIAATN
RDLKQEVQRGRFREDLYFRLNVFPIESVPLRDRREDIPLLAQHFLASERRELKSGLRLSQGDVRRLMRYEWPGNVRELQN
VIERATILAQNGRLRIDLPEPSGHHPAPNAGRQKSETRPAVMTAAELRDLERANIVAALRACNGKVFGDDGAAAMLDLKP
TTLASRIKALGISATRAANGSAVD

Sequences:

>Translated_664_residues
MLRNLVVYDFSESNYEISEQVVDLPVPFSATDTDLRAEAFDGLIEAALLLDPAADQILEVNPAACALLGYDRATLLQTRI
SALHDRQFPALIVFTQAVFDRGSYWTHALTPNHGAGTPLRVEYAGRALQSRGRTLLLLTMSDLEQRRRRHIDAAADDYMR
DGLPAWQRVERVFEDIERENQLILRAAGEGIYGVNAEGRATFVNPAAERMLGWSAEELVGRSIHAVMHHTHHDGRPYADH
DCPIYAAFRDGAVHTVDGEVFWRKDGKPVWVEYTSTPIRDRSGVIVGAVVVFRDVSQRREADEKLHAALAEVDRLRERLQ
LENDYLQEEIRIETNPRGIIGQSEAIQTTLRQVKLVAPTTAAVLITGESGTGKELIARAIHDASTRSGRPLIRVNCAAIP
RELFESEFFGHTRGAFTGAVRDRIGRFELADGGTLFLDEIGEIPLELQGKLLRVLQEGNFERVGDERTRNVDVRLIAATN
RDLKQEVQRGRFREDLYFRLNVFPIESVPLRDRREDIPLLAQHFLASERRELKSGLRLSQGDVRRLMRYEWPGNVRELQN
VIERATILAQNGRLRIDLPEPSGHHPAPNAGRQKSETRPAVMTAAELRDLERANIVAALRACNGKVFGDDGAAAMLDLKP
TTLASRIKALGISATRAANGSAVD
>Mature_664_residues
MLRNLVVYDFSESNYEISEQVVDLPVPFSATDTDLRAEAFDGLIEAALLLDPAADQILEVNPAACALLGYDRATLLQTRI
SALHDRQFPALIVFTQAVFDRGSYWTHALTPNHGAGTPLRVEYAGRALQSRGRTLLLLTMSDLEQRRRRHIDAAADDYMR
DGLPAWQRVERVFEDIERENQLILRAAGEGIYGVNAEGRATFVNPAAERMLGWSAEELVGRSIHAVMHHTHHDGRPYADH
DCPIYAAFRDGAVHTVDGEVFWRKDGKPVWVEYTSTPIRDRSGVIVGAVVVFRDVSQRREADEKLHAALAEVDRLRERLQ
LENDYLQEEIRIETNPRGIIGQSEAIQTTLRQVKLVAPTTAAVLITGESGTGKELIARAIHDASTRSGRPLIRVNCAAIP
RELFESEFFGHTRGAFTGAVRDRIGRFELADGGTLFLDEIGEIPLELQGKLLRVLQEGNFERVGDERTRNVDVRLIAATN
RDLKQEVQRGRFREDLYFRLNVFPIESVPLRDRREDIPLLAQHFLASERRELKSGLRLSQGDVRRLMRYEWPGNVRELQN
VIERATILAQNGRLRIDLPEPSGHHPAPNAGRQKSETRPAVMTAAELRDLERANIVAALRACNGKVFGDDGAAAMLDLKP
TTLASRIKALGISATRAANGSAVD

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=344, Percent_Identity=52.0348837209302, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1789087, Length=350, Percent_Identity=52, Blast_Score=318, Evalue=8e-88,
Organism=Escherichia coli, GI1790437, Length=292, Percent_Identity=50.3424657534247, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082152, Length=238, Percent_Identity=54.2016806722689, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788550, Length=317, Percent_Identity=47.0031545741325, Blast_Score=253, Evalue=4e-68,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=44.8795180722892, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788905, Length=235, Percent_Identity=51.4893617021277, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1789233, Length=238, Percent_Identity=45.3781512605042, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI87081872, Length=233, Percent_Identity=48.068669527897, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1786524, Length=247, Percent_Identity=46.1538461538462, Blast_Score=202, Evalue=7e-53,
Organism=Escherichia coli, GI1787583, Length=306, Percent_Identity=38.8888888888889, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI87081858, Length=293, Percent_Identity=36.8600682593857, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI1789828, Length=277, Percent_Identity=33.9350180505415, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788381, Length=140, Percent_Identity=30.7142857142857, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 74199; Mature: 74199

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRNLVVYDFSESNYEISEQVVDLPVPFSATDTDLRAEAFDGLIEAALLLDPAADQILEV
CCCCEEEEECCCCCCCHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEC
NPAACALLGYDRATLLQTRISALHDRQFPALIVFTQAVFDRGSYWTHALTPNHGAGTPLR
CCHHEEEECCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEECCCCCCCCCCEE
VEYAGRALQSRGRTLLLLTMSDLEQRRRRHIDAAADDYMRDGLPAWQRVERVFEDIEREN
EHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCC
QLILRAAGEGIYGVNAEGRATFVNPAAERMLGWSAEELVGRSIHAVMHHTHHDGRPYADH
EEEEEECCCCEEECCCCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
DCPIYAAFRDGAVHTVDGEVFWRKDGKPVWVEYTSTPIRDRSGVIVGAVVVFRDVSQRRE
CCCEEEEECCCEEEEECCEEEEECCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHH
ADEKLHAALAEVDRLRERLQLENDYLQEEIRIETNPRGIIGQSEAIQTTLRQVKLVAPTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHEEECCCE
AAVLITGESGTGKELIARAIHDASTRSGRPLIRVNCAAIPRELFESEFFGHTRGAFTGAV
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHH
RDRIGRFELADGGTLFLDEIGEIPLELQGKLLRVLQEGNFERVGDERTRNVDVRLIAATN
HHHCCCEEECCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEEEEECC
RDLKQEVQRGRFREDLYFRLNVFPIESVPLRDRREDIPLLAQHFLASERRELKSGLRLSQ
HHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCH
GDVRRLMRYEWPGNVRELQNVIERATILAQNGRLRIDLPEPSGHHPAPNAGRQKSETRPA
HHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCH
VMTAAELRDLERANIVAALRACNGKVFGDDGAAAMLDLKPTTLASRIKALGISATRAANG
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCC
SAVD
CCCC
>Mature Secondary Structure
MLRNLVVYDFSESNYEISEQVVDLPVPFSATDTDLRAEAFDGLIEAALLLDPAADQILEV
CCCCEEEEECCCCCCCHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEC
NPAACALLGYDRATLLQTRISALHDRQFPALIVFTQAVFDRGSYWTHALTPNHGAGTPLR
CCHHEEEECCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEECCCCCCCCCCEE
VEYAGRALQSRGRTLLLLTMSDLEQRRRRHIDAAADDYMRDGLPAWQRVERVFEDIEREN
EHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCC
QLILRAAGEGIYGVNAEGRATFVNPAAERMLGWSAEELVGRSIHAVMHHTHHDGRPYADH
EEEEEECCCCEEECCCCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
DCPIYAAFRDGAVHTVDGEVFWRKDGKPVWVEYTSTPIRDRSGVIVGAVVVFRDVSQRRE
CCCEEEEECCCEEEEECCEEEEECCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHH
ADEKLHAALAEVDRLRERLQLENDYLQEEIRIETNPRGIIGQSEAIQTTLRQVKLVAPTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHEEECCCE
AAVLITGESGTGKELIARAIHDASTRSGRPLIRVNCAAIPRELFESEFFGHTRGAFTGAV
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHH
RDRIGRFELADGGTLFLDEIGEIPLELQGKLLRVLQEGNFERVGDERTRNVDVRLIAATN
HHHCCCEEECCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEEEEECC
RDLKQEVQRGRFREDLYFRLNVFPIESVPLRDRREDIPLLAQHFLASERRELKSGLRLSQ
HHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCH
GDVRRLMRYEWPGNVRELQNVIERATILAQNGRLRIDLPEPSGHHPAPNAGRQKSETRPA
HHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCH
VMTAAELRDLERANIVAALRACNGKVFGDDGAAAMLDLKPTTLASRIKALGISATRAANG
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCC
SAVD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]