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The map label for this gene is bcr [H]

Identifier: 86749132

GI number: 86749132

Start: 2285847

End: 2287136

Strand: Reverse

Name: bcr [H]

Synonym: RPB_2009

Alternate gene names: 86749132

Gene position: 2287136-2285847 (Counterclockwise)

Preceding gene: 86749133

Following gene: 86749122

Centisome position: 42.9

GC content: 68.06

Gene sequence:

>1290_bases
ATGCACGGCAAGACCGGTAGAACCTTCGCGATCGACCCCGGCCGGATCGACCGGCCGCCGTCGAAACTGCTGCTGCTCCT
GCTGGTGCTGATGAACGGCGCGGCGCCGATCGCGCTGTACATCTTCGTGCCGGCGCTGCCGGTGCTCGCCAGCGATCTCG
GCGGCGACATCTCGGTCGCGCAGATGACGGTGTCGCTCTACATGGTCGGGCTGGCGTGCTCGCAACTGATCATGGGCCCG
CTGTCGGACCGGTTTGGCCGCCGCCCGGTGCTGCTCGGCGGCTTGACGCTGATGGTCGCCGCCAGCGTCGGCTGCATCTT
CGCCGAGACCCTGCCGCAACTGATCGCCGCGCGGTTCCTGCAGGCGCTCGGCGGCGCTTCCGGCATGGTGATCAGCCGCG
CCATCATCCGCGATCTGTACAGCCGCGACCGCGTCGGCGGCATGCTCAGTCTCGTCATCGCCGTGATGATGATCGCGCAG
ATGCTGAGCCCGCTGTTCGGCGGCGTGATCGAAACCGCGCTCGGCTGGCGCGCGATCTTCTACGTCGTCACGGCGGGCGC
GATCGCCGTCACGGCGACGATCGCGCTGGCGCTGCCCGAGACGCGCCGCCGCCTCGGTCCCGCCGCACCGGGCGGCTTCC
GCGGCGATGTCGGCGGCCTGTTCAGGAGCCGCGCCTTCATCGGCTATGTGCTTTGCCAGGTGCTGGCCTCGGCGATCATC
TTCACCTTCGCCGGCGGCGGGCCGTACGTCGTGGTGACGCAGATGGGCCGCAGTTCGGCCGAATACGGCGCCTGGTTCGC
CAGCTCCGGCTTCGCCTATCTGCTCGGCAATCTGTTCTGCGTGCGGTTCGCGCCGCGCTATTCGCTCGACAAACTGATCT
GGTTCGGACTGGCGATGCAGATCGGCGGCGCGGCGCTGAATCTGGCGTGGGGCGTGCTCGGCTGGAATCAGGTGCCGAGC
TGGCTGTTCGCCACCCACATGATCATCATGTTCGGCAACGCCTTCGTGATGGCCAATGCAACCGCCGGCGCGATCAGCAT
AAGGCCGCAGGTGGCCGGCACCGCTTCCGGGCTGATGGGCTTCACCCAATTCGGGATCGGCGCGCTGTGCTCGCAGTTCG
GCGCGTATCTCGGCGGCCATTTCGCCACGCCGCTGCCGCTCAACATCGCCGTCGCCGCCCTCGCGCTGGCCTGCGCCGCC
TCGATGATCTTCCTGGTGCCACGCAGCAACCTCATCGCGAGCGAAGAGCTGATCGAGAAGGCAGAAGGCGAAGAGCCGCC
GGTGATGTGA

Upstream 100 bases:

>100_bases
GGCCTTATGCCGAAAACGGCCTGTTAGTGATCCCGCGGCACGTTACAGTCGAGTGCACACCGCACATCGTCATTTGCTGA
CCGGTCGGAACCGCCCCCGC

Downstream 100 bases:

>100_bases
GGGCGGCCTGCCTGCGCCGTTTCACGCACAGCATCGTCATCCTGAGGTGCGCGCCCTCCTCGGCGCGCCTCGAAGGATGC
GGCGACGCGCGCAAGCGGCC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MHGKTGRTFAIDPGRIDRPPSKLLLLLLVLMNGAAPIALYIFVPALPVLASDLGGDISVAQMTVSLYMVGLACSQLIMGP
LSDRFGRRPVLLGGLTLMVAASVGCIFAETLPQLIAARFLQALGGASGMVISRAIIRDLYSRDRVGGMLSLVIAVMMIAQ
MLSPLFGGVIETALGWRAIFYVVTAGAIAVTATIALALPETRRRLGPAAPGGFRGDVGGLFRSRAFIGYVLCQVLASAII
FTFAGGGPYVVVTQMGRSSAEYGAWFASSGFAYLLGNLFCVRFAPRYSLDKLIWFGLAMQIGGAALNLAWGVLGWNQVPS
WLFATHMIIMFGNAFVMANATAGAISIRPQVAGTASGLMGFTQFGIGALCSQFGAYLGGHFATPLPLNIAVAALALACAA
SMIFLVPRSNLIASEELIEKAEGEEPPVM

Sequences:

>Translated_429_residues
MHGKTGRTFAIDPGRIDRPPSKLLLLLLVLMNGAAPIALYIFVPALPVLASDLGGDISVAQMTVSLYMVGLACSQLIMGP
LSDRFGRRPVLLGGLTLMVAASVGCIFAETLPQLIAARFLQALGGASGMVISRAIIRDLYSRDRVGGMLSLVIAVMMIAQ
MLSPLFGGVIETALGWRAIFYVVTAGAIAVTATIALALPETRRRLGPAAPGGFRGDVGGLFRSRAFIGYVLCQVLASAII
FTFAGGGPYVVVTQMGRSSAEYGAWFASSGFAYLLGNLFCVRFAPRYSLDKLIWFGLAMQIGGAALNLAWGVLGWNQVPS
WLFATHMIIMFGNAFVMANATAGAISIRPQVAGTASGLMGFTQFGIGALCSQFGAYLGGHFATPLPLNIAVAALALACAA
SMIFLVPRSNLIASEELIEKAEGEEPPVM
>Mature_429_residues
MHGKTGRTFAIDPGRIDRPPSKLLLLLLVLMNGAAPIALYIFVPALPVLASDLGGDISVAQMTVSLYMVGLACSQLIMGP
LSDRFGRRPVLLGGLTLMVAASVGCIFAETLPQLIAARFLQALGGASGMVISRAIIRDLYSRDRVGGMLSLVIAVMMIAQ
MLSPLFGGVIETALGWRAIFYVVTAGAIAVTATIALALPETRRRLGPAAPGGFRGDVGGLFRSRAFIGYVLCQVLASAII
FTFAGGGPYVVVTQMGRSSAEYGAWFASSGFAYLLGNLFCVRFAPRYSLDKLIWFGLAMQIGGAALNLAWGVLGWNQVPS
WLFATHMIIMFGNAFVMANATAGAISIRPQVAGTASGLMGFTQFGIGALCSQFGAYLGGHFATPLPLNIAVAALALACAA
SMIFLVPRSNLIASEELIEKAEGEEPPVM

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=361, Percent_Identity=32.409972299169, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI48994889, Length=261, Percent_Identity=30.6513409961686, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1790146, Length=232, Percent_Identity=32.3275862068966, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082312, Length=344, Percent_Identity=29.9418604651163, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790794, Length=299, Percent_Identity=24.0802675585284, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787065, Length=272, Percent_Identity=26.1029411764706, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790195, Length=142, Percent_Identity=30.2816901408451, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=165, Percent_Identity=30.3030303030303, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322071, Length=198, Percent_Identity=28.7878787878788, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322070, Length=221, Percent_Identity=28.9592760180996, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319517, Length=192, Percent_Identity=26.0416666666667, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321838, Length=147, Percent_Identity=26.530612244898, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45022; Mature: 45022

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCC
>Mature Secondary Structure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HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]