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The map label for this gene is aam1 [H]

Identifier: 86749007

GI number: 86749007

Start: 2154499

End: 2156553

Strand: Reverse

Name: aam1 [H]

Synonym: RPB_1884

Alternate gene names: 86749007

Gene position: 2156553-2154499 (Counterclockwise)

Preceding gene: 86749010

Following gene: 86749006

Centisome position: 40.45

GC content: 62.48

Gene sequence:

>2055_bases
GTGAGTTCCGGGAACTTTGCCGTTCCTGAGGCTTTGTTACGAGACGACCGGGCGTTGTTCGACTTCCAGGGAAATAGGGC
TACGGTCCTATCGCATCACGTGAACAGATCGACTCAAGCATTCCAGACCGTCTCGGCTGGCGGCGCTTTCCAAATTGAAG
ATATCTTCCCGATCATCGACAGCGGCCGATTTCCCGTCAAGCGGGTTGTCGGCGAGCCCATCGAGATCTGGGCGGACGTG
TATCGCGACGGTCACGAGGTGATCGCTGCGGCGCTGGTCTGGCGGCGCGAGCAGGATCAGGATTGGCAGCGCGTCCCGAT
GCGTCACGTCGTCAACGATCGCTGGACCGCCTCCTTCGTGCCCGATCATATTGGACGATATGTCTATGCGATCGAAGCGT
GGACCGACGAATTCGCGACCTGGCGGCACGGCATCGAGCTGAAGCAGAAGGCGGGGCAAGACGTCGCACTCGACGCGCTG
GAAGGCGCCGGGATGCTGACCAAAGCTCTGAACGGCGGGCACGAAGCGCTGACCGTTATTCAGCGGCAATGCGAGGACTA
TCTGCAGACCGGCGACGTGGCGCCTTTGCTCACCCCCGAATTGCGCGACGCGATGGCCGAAAGCCAGGCGCGGCCGGACG
TCACCCGCTCCGCGCTGCTGCCGCTGATGATCGACCGCGAACGCGCCCGCTGCGGAGCGTGGTACGAGATGGTGCCGCGC
AGCCAGAGCACGGTGCCCGGCCAGCACGGCACCTTCCGCGACTGCATCGCAAGGTTGCCGGACATCGCGGCGATGGGCTT
CGATGTGCTGTATTTCACGCCGATCCACCCGATCGGCCGCGTCAATCGCAAAGGCCGCAACAACTCGCTGAAGGCCGACC
CCGGCGATCCGGGCAGCCCCTATGCGATCGGTGCTGAGGAAGGCGGCCACGACGCGGTGCATCCCGAGCTCGGCACGCTC
GACGATTTCCACGCGCTGGTCGAAGCCTGCAAGCTGGTGAACGTCGAGATCGCGCTCGACATCGCGGTGCAATGCTCGCC
GGATCACCCGTGGCTGAAGCAGCATCCGGACTGGTTCAAGCGCCGTCCCGACGGCTCGATGAAATACGCCGAGAACCCGC
CGAAGAAATACGAGGACATCGTCAATCCGGATTTCACCTGCGAGGACGCCGGCTCGCTGTGGAATGCGCTGCGCGACGTG
ATCCTGTTCTGGGTCGACCACGGCGTGAAGATCTTTCGGGTGGACAACCCGCACACCAAGCCGCTGCGGTTCTGGGAATG
GCTGATCCACGAGGTTCAGCTCGCTCACCCCGACGTGCTGTTTCTCGCCGAGGCCTTCACCCGGCCGAAGCTGATGAAGG
GGCTCGCCAAACTCGGCTTCAGCCAGTCCTACACCTACTTCACCTGGCGGACGCAGAAGTGGGAGATCGAGGAATACTTG
CGCGAACTGACAGGCTATCCGGAGCGTGATTTCTACCGCCCGAATTTCTTCGTCAATACCCCGGACATCCTCCCCTACCA
TCTGCAGGGCGGCGAGCCGTGGATGTTCAAATCCCGCCTTGCGCTCGCCGCGATGCTGTCATCGACCTATGGCATCTATA
ACGGCTTCGAGCTGATCGAACACGCGCCGATCCCCGGCAAGGAGGAGTATCTCGATTCCGAAAAATACGAGATCAAGGTC
CGCGACTGGGACAGACCGGGCAATATCAAGCCTTATATCCGCGAGATCAATCAGGCCCGCCGCAACAATCCGGCGCTTCA
GCAGACGAGCAATCTTCGCTTCGTGGATATCCAGGATCCCAACGTGACCGGCTTCGTCAAATACTCAACGGACCTGACCA
ACGTCGTGGCCGTCGCGATCGCGCTGTCGCGCGATTTCCATGAATTCTGGTTTCCGCTCGGCGACACCCAGGTCGAGGTC
GGCGGCGAGCGCCGCCCGGTCGTGGCGGTCGAGAATCTGCTCACCGGCGAGCGGCACGCCGTGGAATGGGGCGGCCTCAC
GCTACGGATCGATCCGCAACGCGATCCTGCGCTGCTTTTCCGCTGCCTGGCGTGA

Upstream 100 bases:

>100_bases
CAATAAATCCATCAGGCACGCTGTCTGCGTCGCCGCGCGGGGTGCGCGGCGAATCCGCCAATTCCGAAGAGGATAGTCGT
CTGCAACGCGATTGCGTAGA

Downstream 100 bases:

>100_bases
GGAGCGCAACCATGAACGTGATGTCGCCGATCGACTCGACCGATTCCCGCGCCCAAGCCGACGCCACCAACGAGCTTTGG
TACAAGGACGCGATCATCTA

Product: alpha amylase, catalytic region

Products: D-glucose; cholesterol

Alternate protein names: NA

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MSSGNFAVPEALLRDDRALFDFQGNRATVLSHHVNRSTQAFQTVSAGGAFQIEDIFPIIDSGRFPVKRVVGEPIEIWADV
YRDGHEVIAAALVWRREQDQDWQRVPMRHVVNDRWTASFVPDHIGRYVYAIEAWTDEFATWRHGIELKQKAGQDVALDAL
EGAGMLTKALNGGHEALTVIQRQCEDYLQTGDVAPLLTPELRDAMAESQARPDVTRSALLPLMIDRERARCGAWYEMVPR
SQSTVPGQHGTFRDCIARLPDIAAMGFDVLYFTPIHPIGRVNRKGRNNSLKADPGDPGSPYAIGAEEGGHDAVHPELGTL
DDFHALVEACKLVNVEIALDIAVQCSPDHPWLKQHPDWFKRRPDGSMKYAENPPKKYEDIVNPDFTCEDAGSLWNALRDV
ILFWVDHGVKIFRVDNPHTKPLRFWEWLIHEVQLAHPDVLFLAEAFTRPKLMKGLAKLGFSQSYTYFTWRTQKWEIEEYL
RELTGYPERDFYRPNFFVNTPDILPYHLQGGEPWMFKSRLALAAMLSSTYGIYNGFELIEHAPIPGKEEYLDSEKYEIKV
RDWDRPGNIKPYIREINQARRNNPALQQTSNLRFVDIQDPNVTGFVKYSTDLTNVVAVAIALSRDFHEFWFPLGDTQVEV
GGERRPVVAVENLLTGERHAVEWGGLTLRIDPQRDPALLFRCLA

Sequences:

>Translated_684_residues
MSSGNFAVPEALLRDDRALFDFQGNRATVLSHHVNRSTQAFQTVSAGGAFQIEDIFPIIDSGRFPVKRVVGEPIEIWADV
YRDGHEVIAAALVWRREQDQDWQRVPMRHVVNDRWTASFVPDHIGRYVYAIEAWTDEFATWRHGIELKQKAGQDVALDAL
EGAGMLTKALNGGHEALTVIQRQCEDYLQTGDVAPLLTPELRDAMAESQARPDVTRSALLPLMIDRERARCGAWYEMVPR
SQSTVPGQHGTFRDCIARLPDIAAMGFDVLYFTPIHPIGRVNRKGRNNSLKADPGDPGSPYAIGAEEGGHDAVHPELGTL
DDFHALVEACKLVNVEIALDIAVQCSPDHPWLKQHPDWFKRRPDGSMKYAENPPKKYEDIVNPDFTCEDAGSLWNALRDV
ILFWVDHGVKIFRVDNPHTKPLRFWEWLIHEVQLAHPDVLFLAEAFTRPKLMKGLAKLGFSQSYTYFTWRTQKWEIEEYL
RELTGYPERDFYRPNFFVNTPDILPYHLQGGEPWMFKSRLALAAMLSSTYGIYNGFELIEHAPIPGKEEYLDSEKYEIKV
RDWDRPGNIKPYIREINQARRNNPALQQTSNLRFVDIQDPNVTGFVKYSTDLTNVVAVAIALSRDFHEFWFPLGDTQVEV
GGERRPVVAVENLLTGERHAVEWGGLTLRIDPQRDPALLFRCLA
>Mature_683_residues
SSGNFAVPEALLRDDRALFDFQGNRATVLSHHVNRSTQAFQTVSAGGAFQIEDIFPIIDSGRFPVKRVVGEPIEIWADVY
RDGHEVIAAALVWRREQDQDWQRVPMRHVVNDRWTASFVPDHIGRYVYAIEAWTDEFATWRHGIELKQKAGQDVALDALE
GAGMLTKALNGGHEALTVIQRQCEDYLQTGDVAPLLTPELRDAMAESQARPDVTRSALLPLMIDRERARCGAWYEMVPRS
QSTVPGQHGTFRDCIARLPDIAAMGFDVLYFTPIHPIGRVNRKGRNNSLKADPGDPGSPYAIGAEEGGHDAVHPELGTLD
DFHALVEACKLVNVEIALDIAVQCSPDHPWLKQHPDWFKRRPDGSMKYAENPPKKYEDIVNPDFTCEDAGSLWNALRDVI
LFWVDHGVKIFRVDNPHTKPLRFWEWLIHEVQLAHPDVLFLAEAFTRPKLMKGLAKLGFSQSYTYFTWRTQKWEIEEYLR
ELTGYPERDFYRPNFFVNTPDILPYHLQGGEPWMFKSRLALAAMLSSTYGIYNGFELIEHAPIPGKEEYLDSEKYEIKVR
DWDRPGNIKPYIREINQARRNNPALQQTSNLRFVDIQDPNVTGFVKYSTDLTNVVAVAIALSRDFHEFWFPLGDTQVEVG
GERRPVVAVENLLTGERHAVEWGGLTLRIDPQRDPALLFRCLA

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF11896 DUF3416 [H]

EC number: 3.2.1.1

Molecular weight: Translated: 77950; Mature: 77819

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGNFAVPEALLRDDRALFDFQGNRATVLSHHVNRSTQAFQTVSAGGAFQIEDIFPIID
CCCCCCCCCHHHHCCCCEEEEECCCEEEEEEHHHCCHHHHHHHHCCCCCEEECCHHHHHC
SGRFPVKRVVGEPIEIWADVYRDGHEVIAAALVWRREQDQDWQRVPMRHVVNDRWTASFV
CCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHCCHHHHHCCCCEECCC
PDHIGRYVYAIEAWTDEFATWRHGIELKQKAGQDVALDALEGAGMLTKALNGGHEALTVI
HHHHCCEEEEEEHHHHHHHHHHHCCHHHHHCCCCEEHHHHCCCHHHHHHHCCCHHHHHHH
QRQCEDYLQTGDVAPLLTPELRDAMAESQARPDVTRSALLPLMIDRERARCGAWYEMVPR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCHHHHCCC
SQSTVPGQHGTFRDCIARLPDIAAMGFDVLYFTPIHPIGRVNRKGRNNSLKADPGDPGSP
CCCCCCCCCCCHHHHHHHCCCHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
YAIGAEEGGHDAVHPELGTLDDFHALVEACKLVNVEIALDIAVQCSPDHPWLKQHPDWFK
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEEEECCCCCHHHCCCHHHH
RRPDGSMKYAENPPKKYEDIVNPDFTCEDAGSLWNALRDVILFWVDHGVKIFRVDNPHTK
HCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCC
PLRFWEWLIHEVQLAHPDVLFLAEAFTRPKLMKGLAKLGFSQSYTYFTWRTQKWEIEEYL
HHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHH
RELTGYPERDFYRPNFFVNTPDILPYHLQGGEPWMFKSRLALAAMLSSTYGIYNGFELIE
HHHCCCCCCCCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHCCHHHHH
HAPIPGKEEYLDSEKYEIKVRDWDRPGNIKPYIREINQARRNNPALQQTSNLRFVDIQDP
CCCCCCHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHCCCEEEEEECCC
NVTGFVKYSTDLTNVVAVAIALSRDFHEFWFPLGDTQVEVGGERRPVVAVENLLTGERHA
CEEEEEEECCHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCCCCCEEEHHHHHCCCCCE
VEWGGLTLRIDPQRDPALLFRCLA
EEECCEEEEECCCCCHHHHHHHCC
>Mature Secondary Structure 
SSGNFAVPEALLRDDRALFDFQGNRATVLSHHVNRSTQAFQTVSAGGAFQIEDIFPIID
CCCCCCCCHHHHCCCCEEEEECCCEEEEEEHHHCCHHHHHHHHCCCCCEEECCHHHHHC
SGRFPVKRVVGEPIEIWADVYRDGHEVIAAALVWRREQDQDWQRVPMRHVVNDRWTASFV
CCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHCCHHHHHCCCCEECCC
PDHIGRYVYAIEAWTDEFATWRHGIELKQKAGQDVALDALEGAGMLTKALNGGHEALTVI
HHHHCCEEEEEEHHHHHHHHHHHCCHHHHHCCCCEEHHHHCCCHHHHHHHCCCHHHHHHH
QRQCEDYLQTGDVAPLLTPELRDAMAESQARPDVTRSALLPLMIDRERARCGAWYEMVPR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCHHHHCCC
SQSTVPGQHGTFRDCIARLPDIAAMGFDVLYFTPIHPIGRVNRKGRNNSLKADPGDPGSP
CCCCCCCCCCCHHHHHHHCCCHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
YAIGAEEGGHDAVHPELGTLDDFHALVEACKLVNVEIALDIAVQCSPDHPWLKQHPDWFK
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEEEECCCCCHHHCCCHHHH
RRPDGSMKYAENPPKKYEDIVNPDFTCEDAGSLWNALRDVILFWVDHGVKIFRVDNPHTK
HCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCC
PLRFWEWLIHEVQLAHPDVLFLAEAFTRPKLMKGLAKLGFSQSYTYFTWRTQKWEIEEYL
HHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHH
RELTGYPERDFYRPNFFVNTPDILPYHLQGGEPWMFKSRLALAAMLSSTYGIYNGFELIE
HHHCCCCCCCCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHCCHHHHH
HAPIPGKEEYLDSEKYEIKVRDWDRPGNIKPYIREINQARRNNPALQQTSNLRFVDIQDP
CCCCCCHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHCCCEEEEEECCC
NVTGFVKYSTDLTNVVAVAIALSRDFHEFWFPLGDTQVEVGGERRPVVAVENLLTGERHA
CEEEEEEECCHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCCCCCEEEHHHHHCCCCCE
VEWGGLTLRIDPQRDPALLFRCLA
EEECCEEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; cholesteryl-beta-D-glucoside

Specific reaction: H2O + cholesteryl-beta-D-glucoside = D-glucose + cholesterol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10970853 [H]