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The map label for this gene is glgB

Identifier: 86749005

GI number: 86749005

Start: 2149038

End: 2151188

Strand: Reverse

Name: glgB

Synonym: RPB_1882

Alternate gene names: 86749005

Gene position: 2151188-2149038 (Counterclockwise)

Preceding gene: 86749006

Following gene: 86749004

Centisome position: 40.35

GC content: 65.18

Gene sequence:

>2151_bases
ATGACCGTCCAATTGACCGATGACGCCTATGCGGTGCTCGAAGGCCGCCACGCGGACCCGTTTCGCTATCTCGGCCCGCA
TCCCGAGGACGACCGCGTCGTCGTCCGGGCGCTGTTGCCGGATGCGACCGCGGTCGAGGCGGTCGGCGAGCATGGCGAGA
CGGCCATCCTCGAGCGGGTTCACGATGCCGGTCTGTTCGCCGGGCCGCTGCCGAACGGCTCACATCGCTATCAGCTCCGC
GCCCGGTTCGGCGACACTACCGTGGATCTCGAAGATCCGTATCGCTTTCCCCCGATCCTGACCGAGTTCGATCTGTATCT
GCTGGGCGAAGGCACCGATCAGCGGCTGTACGACAAGCTCGGCGCGCACCCGATGGTGCTGGAAGGCGTCAGAGGCGTCG
GCTTCGTGGTGCTGGCGCCCAATGCCCGCCGCGTCAGCGTGGTCGGCGACTTCAACTTCTGGAATCCGCGCCGGCACCAG
ATGCGGGTGCGCGGCAACGGCTATTGGGAGCTGTTCATCCCCGGCGCCACCGCCGGCGATCACTACAAATTCGACATGAC
CGGGCCGAACGGCGAGACGCTGCCGCAAAAGTCCGATCCGATGGCGTTCGCCGCCGAGATGCGGCCGAAGACGGCCTCGA
TCGTGGTGGATCAGACCCGGCTGCCGCTGCCACGCCCGGCGCCGGACAACATCAACGCGCTCGGCGCGCCGATGTCGATC
TACGAGGTGCATCTGGGCTCGTGGCGCCGCAAGGACGGCGAGCAGTGGCTGACCTATCGCGAGCTCGCCGAGCAATTGCC
GGCCTATGTCCGCGACATGGGCTTCACCCATGTCGAATTTTTGCCGGTCAGCGAGCATCCGTTCGACGGCTCCTGGGGCT
ATCAGCCGACCGGGCTGTTTGCGCCGACCAGCCGGTTCGGCACGCCGGAGGATTTCTGCGCGCTGATCGACGCCTGCCAT
GAGCACGGCATCGGCGTGCTGCTCGACTGGGTCCCCGGCCACTTCCCCGACGATCCGCACGGGCTCGGCAATTTCGACGG
CACCGCGCTGTACGAGCACGCCAACCCGCTGCAGGGCCGGCATCTGGACTGGGGCACGCTGATCTACAATTACGGCCGCA
CCGAAGTGGTGAACTTCCTGGTGTCGAACGCGCTGTTCTGGCTGGAACGCTACCGCATCGACGGGTTGCGGGTCGATGCG
GTGGCCTCGATGCTGTATCTCGACTACAGCCGGCCGGCGGGCGGCTGGATCCCGAACAAGTTCGGCGGCCGCGAGAACAT
CGAGGCGATCGACTTCCTGCGCCGCTTCAACGCCGAGGTCTACGCCAAATTCCCGCAGGCCACCACGGCAGCCGAGGAAT
CCACCGCCTGGCCGCAGGTGTCGCGCCCGGTCGAGTTCGGCGGGCTCGGCTTCGGCTACAAGTGGAACATGGGCTGGATG
CACGACACGCTGAACTACATCAGCAAGGATCCGATCCACCGCAAATATCACCACGGCCAGATCCTGTTCGGGCTGCACTA
CGCATTCTCGGAGAACTTCATCCTGCCGCTGTCGCACGACGAGGTCGTGCACGGCAAACGCTCGATCCTCGGCCGGATGC
CGGGCGACGAATGGCAGCGCTTCGCCAATCTGCGCGCCTACTACGCCTTCATGTTCGCGCATCCGGGCAAGAAGCTGATG
TTCATGGGCAGCGAATTCGGCCAGGAGCGGGAGTGGAATCACGATCGCTCACTCGACTGGCACCTGCTCGACACCCCGAA
ATACGCCGGCATCCAGGCGCTGGTCCGCGACCTCAACAGACTGTACCGGGACTTGCCGGCGCTGCATCAGCTCGATTGCG
ATCCGTTCGGCTTCGAATGGATCATCACCGAAGACGCCGCGCGCAACGTGTTCGCCTGGATGCGGAAGGGCAACGACACC
CGGGCGCGCTGCCTGGTGATCGTGAATTTCTCCCCGAACGTCTATCAGGACTACCGCGTCCGTGTGCCCTTCCCCGGCCG
CTGGCGCGAGGTGCTGAATTCCGACGCCGCGATTTACGGCGGCAGCAATGTCGGCAACGCCGGCGAGGTTCGGACTCTCG
AGGGATTGGTGCCCGAGCTCAGTCTGAACATTCCGCCGCTGGCCGCGATCTTTCTGAAGCCGGAAGATTGA

Upstream 100 bases:

>100_bases
TATACGAGATCGAATACGAACTCGCCTATCGTCCCGATTGGCTCCGCGTGCCGCTGGCTGGCATTCTTCGCATCCTGACT
CGGCAGCCCGAGGAGAATTC

Downstream 100 bases:

>100_bases
GCATGCGCCTTACCGCCGGAAGCTGCTCCCGTCTCGGCGCCACCTGGGACGGCCGCGGCACCAATTTCGCGCTGTTCTCG
GCCAATGCCCACAAGGTCGA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MTVQLTDDAYAVLEGRHADPFRYLGPHPEDDRVVVRALLPDATAVEAVGEHGETAILERVHDAGLFAGPLPNGSHRYQLR
ARFGDTTVDLEDPYRFPPILTEFDLYLLGEGTDQRLYDKLGAHPMVLEGVRGVGFVVLAPNARRVSVVGDFNFWNPRRHQ
MRVRGNGYWELFIPGATAGDHYKFDMTGPNGETLPQKSDPMAFAAEMRPKTASIVVDQTRLPLPRPAPDNINALGAPMSI
YEVHLGSWRRKDGEQWLTYRELAEQLPAYVRDMGFTHVEFLPVSEHPFDGSWGYQPTGLFAPTSRFGTPEDFCALIDACH
EHGIGVLLDWVPGHFPDDPHGLGNFDGTALYEHANPLQGRHLDWGTLIYNYGRTEVVNFLVSNALFWLERYRIDGLRVDA
VASMLYLDYSRPAGGWIPNKFGGRENIEAIDFLRRFNAEVYAKFPQATTAAEESTAWPQVSRPVEFGGLGFGYKWNMGWM
HDTLNYISKDPIHRKYHHGQILFGLHYAFSENFILPLSHDEVVHGKRSILGRMPGDEWQRFANLRAYYAFMFAHPGKKLM
FMGSEFGQEREWNHDRSLDWHLLDTPKYAGIQALVRDLNRLYRDLPALHQLDCDPFGFEWIITEDAARNVFAWMRKGNDT
RARCLVIVNFSPNVYQDYRVRVPFPGRWREVLNSDAAIYGGSNVGNAGEVRTLEGLVPELSLNIPPLAAIFLKPED

Sequences:

>Translated_716_residues
MTVQLTDDAYAVLEGRHADPFRYLGPHPEDDRVVVRALLPDATAVEAVGEHGETAILERVHDAGLFAGPLPNGSHRYQLR
ARFGDTTVDLEDPYRFPPILTEFDLYLLGEGTDQRLYDKLGAHPMVLEGVRGVGFVVLAPNARRVSVVGDFNFWNPRRHQ
MRVRGNGYWELFIPGATAGDHYKFDMTGPNGETLPQKSDPMAFAAEMRPKTASIVVDQTRLPLPRPAPDNINALGAPMSI
YEVHLGSWRRKDGEQWLTYRELAEQLPAYVRDMGFTHVEFLPVSEHPFDGSWGYQPTGLFAPTSRFGTPEDFCALIDACH
EHGIGVLLDWVPGHFPDDPHGLGNFDGTALYEHANPLQGRHLDWGTLIYNYGRTEVVNFLVSNALFWLERYRIDGLRVDA
VASMLYLDYSRPAGGWIPNKFGGRENIEAIDFLRRFNAEVYAKFPQATTAAEESTAWPQVSRPVEFGGLGFGYKWNMGWM
HDTLNYISKDPIHRKYHHGQILFGLHYAFSENFILPLSHDEVVHGKRSILGRMPGDEWQRFANLRAYYAFMFAHPGKKLM
FMGSEFGQEREWNHDRSLDWHLLDTPKYAGIQALVRDLNRLYRDLPALHQLDCDPFGFEWIITEDAARNVFAWMRKGNDT
RARCLVIVNFSPNVYQDYRVRVPFPGRWREVLNSDAAIYGGSNVGNAGEVRTLEGLVPELSLNIPPLAAIFLKPED
>Mature_715_residues
TVQLTDDAYAVLEGRHADPFRYLGPHPEDDRVVVRALLPDATAVEAVGEHGETAILERVHDAGLFAGPLPNGSHRYQLRA
RFGDTTVDLEDPYRFPPILTEFDLYLLGEGTDQRLYDKLGAHPMVLEGVRGVGFVVLAPNARRVSVVGDFNFWNPRRHQM
RVRGNGYWELFIPGATAGDHYKFDMTGPNGETLPQKSDPMAFAAEMRPKTASIVVDQTRLPLPRPAPDNINALGAPMSIY
EVHLGSWRRKDGEQWLTYRELAEQLPAYVRDMGFTHVEFLPVSEHPFDGSWGYQPTGLFAPTSRFGTPEDFCALIDACHE
HGIGVLLDWVPGHFPDDPHGLGNFDGTALYEHANPLQGRHLDWGTLIYNYGRTEVVNFLVSNALFWLERYRIDGLRVDAV
ASMLYLDYSRPAGGWIPNKFGGRENIEAIDFLRRFNAEVYAKFPQATTAAEESTAWPQVSRPVEFGGLGFGYKWNMGWMH
DTLNYISKDPIHRKYHHGQILFGLHYAFSENFILPLSHDEVVHGKRSILGRMPGDEWQRFANLRAYYAFMFAHPGKKLMF
MGSEFGQEREWNHDRSLDWHLLDTPKYAGIQALVRDLNRLYRDLPALHQLDCDPFGFEWIITEDAARNVFAWMRKGNDTR
ARCLVIVNFSPNVYQDYRVRVPFPGRWREVLNSDAAIYGGSNVGNAGEVRTLEGLVPELSLNIPPLAAIFLKPED

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=650, Percent_Identity=28.6153846153846, Blast_Score=194, Evalue=3e-49,
Organism=Escherichia coli, GI1789839, Length=717, Percent_Identity=55.9274755927476, Blast_Score=779, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=661, Percent_Identity=27.5340393343419, Blast_Score=186, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI32564391, Length=529, Percent_Identity=29.3005671077505, Blast_Score=171, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320826, Length=623, Percent_Identity=28.2504012841091, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI28573410, Length=597, Percent_Identity=28.4757118927973, Blast_Score=178, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_RHOP2 (Q2IYX0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485501.1
- ProteinModelPortal:   Q2IYX0
- SMR:   Q2IYX0
- STRING:   Q2IYX0
- GeneID:   3908077
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1882
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- ProtClustDB:   PRK05402
- BioCyc:   RPAL316058:RPB_1882-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 81273; Mature: 81141

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: ACT_SITE 294-294 ACT_SITE 329-329 ACT_SITE 334-334 ACT_SITE 397-397 ACT_SITE 399-399 ACT_SITE 452-452 ACT_SITE 519-519 ACT_SITE 520-520

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQLTDDAYAVLEGRHADPFRYLGPHPEDDRVVVRALLPDATAVEAVGEHGETAILERV
CEEEECCCCEEEEECCCCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHH
HDAGLFAGPLPNGSHRYQLRARFGDTTVDLEDPYRFPPILTEFDLYLLGEGTDQRLYDKL
HHCCEEECCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
GAHPMVLEGVRGVGFVVLAPNARRVSVVGDFNFWNPRRHQMRVRGNGYWELFIPGATAGD
CCCCEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCC
HYKFDMTGPNGETLPQKSDPMAFAAEMRPKTASIVVDQTRLPLPRPAPDNINALGAPMSI
CEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEE
YEVHLGSWRRKDGEQWLTYRELAEQLPAYVRDMGFTHVEFLPVSEHPFDGSWGYQPTGLF
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
APTSRFGTPEDFCALIDACHEHGIGVLLDWVPGHFPDDPHGLGNFDGTALYEHANPLQGR
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
HLDWGTLIYNYGRTEVVNFLVSNALFWLERYRIDGLRVDAVASMLYLDYSRPAGGWIPNK
CCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHEECCCCCCCCCCCC
FGGRENIEAIDFLRRFNAEVYAKFPQATTAAEESTAWPQVSRPVEFGGLGFGYKWNMGWM
CCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHH
HDTLNYISKDPIHRKYHHGQILFGLHYAFSENFILPLSHDEVVHGKRSILGRMPGDEWQR
HHHHHHHHCCCCHHHHCCCEEEEEEEEECCCCEEEECCCCHHHHCHHHHHCCCCCHHHHH
FANLRAYYAFMFAHPGKKLMFMGSEFGQEREWNHDRSLDWHLLDTPKYAGIQALVRDLNR
HHHHHHEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
LYRDLPALHQLDCDPFGFEWIITEDAARNVFAWMRKGNDTRARCLVIVNFSPNVYQDYRV
HHHHCCHHHHCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEE
RVPFPGRWREVLNSDAAIYGGSNVGNAGEVRTLEGLVPELSLNIPPLAAIFLKPED
EECCCHHHHHHHCCCCEEECCCCCCCCCCEEEHHHCCCHHCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TVQLTDDAYAVLEGRHADPFRYLGPHPEDDRVVVRALLPDATAVEAVGEHGETAILERV
EEEECCCCEEEEECCCCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHH
HDAGLFAGPLPNGSHRYQLRARFGDTTVDLEDPYRFPPILTEFDLYLLGEGTDQRLYDKL
HHCCEEECCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
GAHPMVLEGVRGVGFVVLAPNARRVSVVGDFNFWNPRRHQMRVRGNGYWELFIPGATAGD
CCCCEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCC
HYKFDMTGPNGETLPQKSDPMAFAAEMRPKTASIVVDQTRLPLPRPAPDNINALGAPMSI
CEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEE
YEVHLGSWRRKDGEQWLTYRELAEQLPAYVRDMGFTHVEFLPVSEHPFDGSWGYQPTGLF
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
APTSRFGTPEDFCALIDACHEHGIGVLLDWVPGHFPDDPHGLGNFDGTALYEHANPLQGR
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
HLDWGTLIYNYGRTEVVNFLVSNALFWLERYRIDGLRVDAVASMLYLDYSRPAGGWIPNK
CCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHEECCCCCCCCCCCC
FGGRENIEAIDFLRRFNAEVYAKFPQATTAAEESTAWPQVSRPVEFGGLGFGYKWNMGWM
CCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHH
HDTLNYISKDPIHRKYHHGQILFGLHYAFSENFILPLSHDEVVHGKRSILGRMPGDEWQR
HHHHHHHHCCCCHHHHCCCEEEEEEEEECCCCEEEECCCCHHHHCHHHHHCCCCCHHHHH
FANLRAYYAFMFAHPGKKLMFMGSEFGQEREWNHDRSLDWHLLDTPKYAGIQALVRDLNR
HHHHHHEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
LYRDLPALHQLDCDPFGFEWIITEDAARNVFAWMRKGNDTRARCLVIVNFSPNVYQDYRV
HHHHCCHHHHCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEE
RVPFPGRWREVLNSDAAIYGGSNVGNAGEVRTLEGLVPELSLNIPPLAAIFLKPED
EECCCHHHHHHHCCCCEEECCCCCCCCCCEEEHHHCCCHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA