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The map label for this gene is glgX [H]

Identifier: 86749004

GI number: 86749004

Start: 2146960

End: 2149035

Strand: Reverse

Name: glgX [H]

Synonym: RPB_1881

Alternate gene names: 86749004

Gene position: 2149035-2146960 (Counterclockwise)

Preceding gene: 86749005

Following gene: 86749003

Centisome position: 40.31

GC content: 64.69

Gene sequence:

>2076_bases
ATGCGCCTTACCGCCGGAAGCTGCTCCCGTCTCGGCGCCACCTGGGACGGCCGCGGCACCAATTTCGCGCTGTTCTCGGC
CAATGCCCACAAGGTCGAATTGTGCCTGTTCGACAGCCAGGGCCGCCGCGAGCTGGAGCGGATCGAGCTGCCGGAGCGCA
CCGAGGACGTTTGGCACGGCTATCTCAACGACGTGTCGCCCGGCCAGCTCTACGGCTACCGGGTGCACGGCCCCTACGAT
CCCGAGCGAGGCCATCGCTTCAACGCCCACAAGCTGCTGCTCGATCCCTATGCCAAGCGGCTGTCCGGCCGGCTGGTGTG
GAGCGATGCGCACTTCGCCTATCGCGCCGGCAGCCCTCGCGAGGATCTGTCGTTCGATCGCCGCGACAACGCCCGCGGCA
TGCCGAAGGCGGTGGTGGTCGACGAGACCGGCGGCTTCTCGCGCCACGAGAGCCGGCCGAACATTCCCTGGGAAGACACC
ATCATCTACGAAGCCCACGTCAAGGGCCTGACGCAGCTGCGCGAAGACGTGCCGCCCGGCCTGCGCGGCACCTTCGGCGG
GCTCGCCGCGCCGGCGATGATCGATCATTTCAAGCGTCTCGGCGTCACCGCGATCGAACTTCTTCCCGTCCACGCGCTTA
TCGATGATCGCGTGCTGGTCGAGAAGAAGCTGGTGAACTACTGGGGCTACAATACGATTTCGTTTTTCGCGCCGGAACCG
CGCTACGCGCCGGACAATCCGCTCGACGCATTCCGCACAACCGTGGCGCGGCTGCACGACGCCGGCATCGAGGTGATCCT
CGATGTCGTCTACAACCACACCGCCGAGGGCAATCATCTCGGCCCGACGCTGTCCTTCCGCGGCATCGACAACGCCTCGT
ATTACTGGCTGATGCCGGACAATCCGCGGTTCTACGATGATTTCACCGGCTGCGGCAGCTCGGTCAATCTCAATCATCCG
CGTGTCCTGCAGATGGTGATGGACAGTCTGCGCTACTGGGTCGAGGTCTGCCACGTCGACGGCTTCCGCTTCGACCTCGC
CACCACGCTGGCGCGTGGACCGAACGGTTACGAGCGCAATTCCGGGTTCTTCACCGCGCTGCGTCAGGACCCGGTGCTGG
CCTCGGTGAAGCTGATCGCCGAGCCGTGGGACCTCGGCATGGGCGGCTATCAGGTCGGCGCGTTCCCGTCGCAATGGTCG
GAATGGAACGACCGCTATCGCAGCGCGATGCGACGCTACTGGAGCGGCGAAGGCAGCCTGATCGGCGAAGTGTCGCGACG
GATGACCGGCTCGTCCGACATCTTCAACCACGACAGCCGGATGCCGCGCGCCAGCATCAACCACGTCACCGTGCACGACG
GTTTCACGCTCGCGGATCTGTTCAGCTACAACGACAAGCACAACCTCGCCAACGGCGAGGACAACCGCGACGGCTCCAAC
GACAATCACAGCAACAATTGCGGCCACGAGGGCCCGACCGACGATCCGACGATCGTCGCGCTGCGCCGCCAGCTCCGCAA
GAACCAGTTGGCCTGTCTGTTTCTGGCCCAGGGCGTTCCGCTGATGCTCGCCGGCGACGAAGTCGGCAACTCGCAGAACG
GCAACAACAACGCCTATTGCCAGGACAACGACATCGGGTGGGTCAATTGGGACGGCCTCAATCGCGAAGACGACATGGTC
GATTTCGTCGGCCTGATGAGCGAGATCCGCCGCCGCTTTCCGCAGATCCGCTCGCGTCGATGGCTCGACGGCCGGCGCGC
CGACGGGTCCTATGGGGTACTGTGGCTGACGCCATCGGCCACCGAGATGACGGAGCAGGATTGGGCGTTTCCGGACGGAC
GGTTTCTCGCCTACGTGCTGAGCCCCTCCGAGCCGGGTGCCGAAGCGATCTTCATCGTGCTCAATTCGGGCGCCGAGAAG
ATCCGGTTTCAGCTGCCGAAATTGCCTGAATATCAGGACTGGCACCAATTGTTCGATACGACCAAGGACAGCGTCCAGGT
CGCGCCGCTACCGGCAGGCAGCGAAACGTCGGCACCGCCGCGCTCGGTGCTGGCGTTTTCCGGAGCGGGCGCATGA

Upstream 100 bases:

>100_bases
GCAACGCCGGCGAGGTTCGGACTCTCGAGGGATTGGTGCCCGAGCTCAGTCTGAACATTCCGCCGCTGGCCGCGATCTTT
CTGAAGCCGGAAGATTGAGC

Downstream 100 bases:

>100_bases
AGGGGCGGAGCTTCGGCCCGCGGCTGACCGAGAACGGCGCCGAATTCCGGCTGTGGGCTCCCAACGCAACGCGCGTCGAC
GTCGTGCTCGATCGTCCGCA

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MRLTAGSCSRLGATWDGRGTNFALFSANAHKVELCLFDSQGRRELERIELPERTEDVWHGYLNDVSPGQLYGYRVHGPYD
PERGHRFNAHKLLLDPYAKRLSGRLVWSDAHFAYRAGSPREDLSFDRRDNARGMPKAVVVDETGGFSRHESRPNIPWEDT
IIYEAHVKGLTQLREDVPPGLRGTFGGLAAPAMIDHFKRLGVTAIELLPVHALIDDRVLVEKKLVNYWGYNTISFFAPEP
RYAPDNPLDAFRTTVARLHDAGIEVILDVVYNHTAEGNHLGPTLSFRGIDNASYYWLMPDNPRFYDDFTGCGSSVNLNHP
RVLQMVMDSLRYWVEVCHVDGFRFDLATTLARGPNGYERNSGFFTALRQDPVLASVKLIAEPWDLGMGGYQVGAFPSQWS
EWNDRYRSAMRRYWSGEGSLIGEVSRRMTGSSDIFNHDSRMPRASINHVTVHDGFTLADLFSYNDKHNLANGEDNRDGSN
DNHSNNCGHEGPTDDPTIVALRRQLRKNQLACLFLAQGVPLMLAGDEVGNSQNGNNNAYCQDNDIGWVNWDGLNREDDMV
DFVGLMSEIRRRFPQIRSRRWLDGRRADGSYGVLWLTPSATEMTEQDWAFPDGRFLAYVLSPSEPGAEAIFIVLNSGAEK
IRFQLPKLPEYQDWHQLFDTTKDSVQVAPLPAGSETSAPPRSVLAFSGAGA

Sequences:

>Translated_691_residues
MRLTAGSCSRLGATWDGRGTNFALFSANAHKVELCLFDSQGRRELERIELPERTEDVWHGYLNDVSPGQLYGYRVHGPYD
PERGHRFNAHKLLLDPYAKRLSGRLVWSDAHFAYRAGSPREDLSFDRRDNARGMPKAVVVDETGGFSRHESRPNIPWEDT
IIYEAHVKGLTQLREDVPPGLRGTFGGLAAPAMIDHFKRLGVTAIELLPVHALIDDRVLVEKKLVNYWGYNTISFFAPEP
RYAPDNPLDAFRTTVARLHDAGIEVILDVVYNHTAEGNHLGPTLSFRGIDNASYYWLMPDNPRFYDDFTGCGSSVNLNHP
RVLQMVMDSLRYWVEVCHVDGFRFDLATTLARGPNGYERNSGFFTALRQDPVLASVKLIAEPWDLGMGGYQVGAFPSQWS
EWNDRYRSAMRRYWSGEGSLIGEVSRRMTGSSDIFNHDSRMPRASINHVTVHDGFTLADLFSYNDKHNLANGEDNRDGSN
DNHSNNCGHEGPTDDPTIVALRRQLRKNQLACLFLAQGVPLMLAGDEVGNSQNGNNNAYCQDNDIGWVNWDGLNREDDMV
DFVGLMSEIRRRFPQIRSRRWLDGRRADGSYGVLWLTPSATEMTEQDWAFPDGRFLAYVLSPSEPGAEAIFIVLNSGAEK
IRFQLPKLPEYQDWHQLFDTTKDSVQVAPLPAGSETSAPPRSVLAFSGAGA
>Mature_691_residues
MRLTAGSCSRLGATWDGRGTNFALFSANAHKVELCLFDSQGRRELERIELPERTEDVWHGYLNDVSPGQLYGYRVHGPYD
PERGHRFNAHKLLLDPYAKRLSGRLVWSDAHFAYRAGSPREDLSFDRRDNARGMPKAVVVDETGGFSRHESRPNIPWEDT
IIYEAHVKGLTQLREDVPPGLRGTFGGLAAPAMIDHFKRLGVTAIELLPVHALIDDRVLVEKKLVNYWGYNTISFFAPEP
RYAPDNPLDAFRTTVARLHDAGIEVILDVVYNHTAEGNHLGPTLSFRGIDNASYYWLMPDNPRFYDDFTGCGSSVNLNHP
RVLQMVMDSLRYWVEVCHVDGFRFDLATTLARGPNGYERNSGFFTALRQDPVLASVKLIAEPWDLGMGGYQVGAFPSQWS
EWNDRYRSAMRRYWSGEGSLIGEVSRRMTGSSDIFNHDSRMPRASINHVTVHDGFTLADLFSYNDKHNLANGEDNRDGSN
DNHSNNCGHEGPTDDPTIVALRRQLRKNQLACLFLAQGVPLMLAGDEVGNSQNGNNNAYCQDNDIGWVNWDGLNREDDMV
DFVGLMSEIRRRFPQIRSRRWLDGRRADGSYGVLWLTPSATEMTEQDWAFPDGRFLAYVLSPSEPGAEAIFIVLNSGAEK
IRFQLPKLPEYQDWHQLFDTTKDSVQVAPLPAGSETSAPPRSVLAFSGAGA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=658, Percent_Identity=45.4407294832827, Blast_Score=555, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77722; Mature: 77722

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTAGSCSRLGATWDGRGTNFALFSANAHKVELCLFDSQGRRELERIELPERTEDVWHG
CCCCCCCHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHH
YLNDVSPGQLYGYRVHGPYDPERGHRFNAHKLLLDPYAKRLSGRLVWSDAHFAYRAGSPR
HHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHCCEEEEECCCEEEECCCCH
EDLSFDRRDNARGMPKAVVVDETGGFSRHESRPNIPWEDTIIYEAHVKGLTQLREDVPPG
HHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCC
LRGTFGGLAAPAMIDHFKRLGVTAIELLPVHALIDDRVLVEKKLVNYWGYNTISFFAPEP
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCC
RYAPDNPLDAFRTTVARLHDAGIEVILDVVYNHTAEGNHLGPTLSFRGIDNASYYWLMPD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECC
NPRFYDDFTGCGSSVNLNHPRVLQMVMDSLRYWVEVCHVDGFRFDLATTLARGPNGYERN
CCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCC
SGFFTALRQDPVLASVKLIAEPWDLGMGGYQVGAFPSQWSEWNDRYRSAMRRYWSGEGSL
CCEEEEECCCCCEEEHEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
IGEVSRRMTGSSDIFNHDSRMPRASINHVTVHDGFTLADLFSYNDKHNLANGEDNRDGSN
HHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCC
DNHSNNCGHEGPTDDPTIVALRRQLRKNQLACLFLAQGVPLMLAGDEVGNSQNGNNNAYC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCCCCCCCCCCCEE
QDNDIGWVNWDGLNREDDMVDFVGLMSEIRRRFPQIRSRRWLDGRRADGSYGVLWLTPSA
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
TEMTEQDWAFPDGRFLAYVLSPSEPGAEAIFIVLNSGAEKIRFQLPKLPEYQDWHQLFDT
HHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHC
TKDSVQVAPLPAGSETSAPPRSVLAFSGAGA
CCCCEEEEECCCCCCCCCCCHHEEEECCCCC
>Mature Secondary Structure
MRLTAGSCSRLGATWDGRGTNFALFSANAHKVELCLFDSQGRRELERIELPERTEDVWHG
CCCCCCCHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHH
YLNDVSPGQLYGYRVHGPYDPERGHRFNAHKLLLDPYAKRLSGRLVWSDAHFAYRAGSPR
HHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHCCEEEEECCCEEEECCCCH
EDLSFDRRDNARGMPKAVVVDETGGFSRHESRPNIPWEDTIIYEAHVKGLTQLREDVPPG
HHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCC
LRGTFGGLAAPAMIDHFKRLGVTAIELLPVHALIDDRVLVEKKLVNYWGYNTISFFAPEP
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCC
RYAPDNPLDAFRTTVARLHDAGIEVILDVVYNHTAEGNHLGPTLSFRGIDNASYYWLMPD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECC
NPRFYDDFTGCGSSVNLNHPRVLQMVMDSLRYWVEVCHVDGFRFDLATTLARGPNGYERN
CCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCC
SGFFTALRQDPVLASVKLIAEPWDLGMGGYQVGAFPSQWSEWNDRYRSAMRRYWSGEGSL
CCEEEEECCCCCEEEHEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
IGEVSRRMTGSSDIFNHDSRMPRASINHVTVHDGFTLADLFSYNDKHNLANGEDNRDGSN
HHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCC
DNHSNNCGHEGPTDDPTIVALRRQLRKNQLACLFLAQGVPLMLAGDEVGNSQNGNNNAYC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCCCCCCCCCCCEE
QDNDIGWVNWDGLNREDDMVDFVGLMSEIRRRFPQIRSRRWLDGRRADGSYGVLWLTPSA
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
TEMTEQDWAFPDGRFLAYVLSPSEPGAEAIFIVLNSGAEKIRFQLPKLPEYQDWHQLFDT
HHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHC
TKDSVQVAPLPAGSETSAPPRSVLAFSGAGA
CCCCEEEEECCCCCCCCCCCHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]