Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 86748970

GI number: 86748970

Start: 2113572

End: 2114327

Strand: Reverse

Name: rsmE [H]

Synonym: RPB_1847

Alternate gene names: 86748970

Gene position: 2114327-2113572 (Counterclockwise)

Preceding gene: 86748974

Following gene: 86748969

Centisome position: 39.66

GC content: 70.77

Gene sequence:

>756_bases
ATGTCCCGATACGAATTCCGAAGTCCCCGCCTCTTTCTCGACGCTGCGCTCGTCGCCGGTGCGCGGGTACCGCTGAACCG
CGACCAGAGCAATTATCTCGGCAATGTGCTGCGGCTCAGTTCCGGTGCGCCGGTGCTGGCCTTCAACGGCCGCGACGGCG
AGTGGCGCGCCGTGCTCGCCGGCCGTAAGCGGCCGGAGGAACTCGAGGTCGCCGAACAAACCCGGCCGCAGGACCGCCTG
CCCGACCTGACCTATGCGTTTGCGCCGCTGAAACACGCCCGGCTGGATTATATGGCCCAGAAAGCCGTCGAAATGGGCGC
CGGGCGGCTGCAGCCGGTGCTGACGCGGCACACCCAGGTGCACCGGCTGAATACCGAGCGGATGCGCGCCAATGTGATCG
AGGCGGCCGAGCAGTGCGGCATCCTCAGCCTCGCCGGTGTCGAGGAACCGGTCGCGCTGGAGCGCTGGCTCGAGACCCGG
GCCGAAAGCGGTCGCCTGCTGGTGTTCTGCGACGAGGACGCCGAGGTCGCCGATCCGGTGGCCGCGCTGCAGGGCGCCCG
CCCCGCGGCGGCGCAGGGAATCGACCTTCTGGTCGGCCCGGAAGGCGGCTTTGCCGCCGACGAGCGCGACGTTTTGCTGC
GCCAGCCGAACATCCTGCGGCTGGCGCTCGGCCCCCGCATCATGCGCGCCGACACCGCCGCGGTCGCGGCCCTGGCGCTG
GTGCAGGCGGTGGCGGGGGACTGGACCGGCCAATAG

Upstream 100 bases:

>100_bases
AACAACCCGTGGAATAGGGCGGCAGCGCGGCGGTTCGCCACGTTTACCTGCTGATTTTGCTGGACCTTTGGACTAAAAGC
CGGTCACTGGAAGCGATCCC

Downstream 100 bases:

>100_bases
GCCGTTCGCGGATCGGCCGGATGGCCTTGACGCATCTCTTGAATGACGGTCGAAACTGTCATTCGGCCGTGTTAAGGGCT
GCGCCGACAACCCTTTGCCC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSRYEFRSPRLFLDAALVAGARVPLNRDQSNYLGNVLRLSSGAPVLAFNGRDGEWRAVLAGRKRPEELEVAEQTRPQDRL
PDLTYAFAPLKHARLDYMAQKAVEMGAGRLQPVLTRHTQVHRLNTERMRANVIEAAEQCGILSLAGVEEPVALERWLETR
AESGRLLVFCDEDAEVADPVAALQGARPAAAQGIDLLVGPEGGFAADERDVLLRQPNILRLALGPRIMRADTAAVAALAL
VQAVAGDWTGQ

Sequences:

>Translated_251_residues
MSRYEFRSPRLFLDAALVAGARVPLNRDQSNYLGNVLRLSSGAPVLAFNGRDGEWRAVLAGRKRPEELEVAEQTRPQDRL
PDLTYAFAPLKHARLDYMAQKAVEMGAGRLQPVLTRHTQVHRLNTERMRANVIEAAEQCGILSLAGVEEPVALERWLETR
AESGRLLVFCDEDAEVADPVAALQGARPAAAQGIDLLVGPEGGFAADERDVLLRQPNILRLALGPRIMRADTAAVAALAL
VQAVAGDWTGQ
>Mature_250_residues
SRYEFRSPRLFLDAALVAGARVPLNRDQSNYLGNVLRLSSGAPVLAFNGRDGEWRAVLAGRKRPEELEVAEQTRPQDRLP
DLTYAFAPLKHARLDYMAQKAVEMGAGRLQPVLTRHTQVHRLNTERMRANVIEAAEQCGILSLAGVEEPVALERWLETRA
ESGRLLVFCDEDAEVADPVAALQGARPAAAQGIDLLVGPEGGFAADERDVLLRQPNILRLALGPRIMRADTAAVAALALV
QAVAGDWTGQ

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=252, Percent_Identity=26.1904761904762, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27377; Mature: 27245

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYEFRSPRLFLDAALVAGARVPLNRDQSNYLGNVLRLSSGAPVLAFNGRDGEWRAVLA
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHEEECCCCCEEEEECCCCCEEEEEC
GRKRPEELEVAEQTRPQDRLPDLTYAFAPLKHARLDYMAQKAVEMGAGRLQPVLTRHTQV
CCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HRLNTERMRANVIEAAEQCGILSLAGVEEPVALERWLETRAESGRLLVFCDEDAEVADPV
HHCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
AALQGARPAAAQGIDLLVGPEGGFAADERDVLLRQPNILRLALGPRIMRADTAAVAALAL
HHHCCCCCCHHCCCEEEECCCCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHH
VQAVAGDWTGQ
HHHHHCCCCCC
>Mature Secondary Structure 
SRYEFRSPRLFLDAALVAGARVPLNRDQSNYLGNVLRLSSGAPVLAFNGRDGEWRAVLA
CCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHEEECCCCCEEEEECCCCCEEEEEC
GRKRPEELEVAEQTRPQDRLPDLTYAFAPLKHARLDYMAQKAVEMGAGRLQPVLTRHTQV
CCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HRLNTERMRANVIEAAEQCGILSLAGVEEPVALERWLETRAESGRLLVFCDEDAEVADPV
HHCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
AALQGARPAAAQGIDLLVGPEGGFAADERDVLLRQPNILRLALGPRIMRADTAAVAALAL
HHHCCCCCCHHCCCEEEECCCCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHH
VQAVAGDWTGQ
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]