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The map label for this gene is ghrA [H]

Identifier: 86748770

GI number: 86748770

Start: 1875994

End: 1876929

Strand: Reverse

Name: ghrA [H]

Synonym: RPB_1647

Alternate gene names: 86748770

Gene position: 1876929-1875994 (Counterclockwise)

Preceding gene: 86748771

Following gene: 86748758

Centisome position: 35.2

GC content: 67.2

Gene sequence:

>936_bases
ATGCGTTGCGCCCTCGTCAGCGACCGTCTCGATCTGCGCGGCTTTCTCGGGACGGATTTCGCGCGCATCGCCGATCGCAT
CGAATTCGTCGATCATCGCACCGACGGCGCGAACGCGGATATCCGCCTGGCAGTCGCCTGGTATCCGCCGGACGACGCCT
TCGCGCACTATCCCAACCTCCGGGCTGTCTGCTCGATCGGCGCCGGCGTGGATAATATCACCGCCTGCCCGAGCCTGCGG
GCGGACATCGACGTCGTTCGAATCGTCGATTCGGCGCAGGCCCAGATGATGTCGGGATTCGTGCTCTGGCATGTGATCTG
GCATCAGCGGCGGTTCGCCACCTATCTGGCGCAGCAGCGCGATGCGGTCTGGCAACGCATTCGTCAACGCAATCCGCGCG
AGGTGCCGGTCGCCATTCTCGGCTACGGCGAGATCGGCCAGAAGGTCGCCGCGGATCTCGCTGCGCTCGGTTTCCCGGTG
ATGGTGTGGAGCCGGTCGCCAAAGTCGACGCCGGCCGCGATCAGGGGATTCCATGGGCGCGAGGGCCTCGCCGCCATGCT
CGGCGAAACCGAGGTGCTGGTGAATCTGCTGCCGCTGACGCAGGAGACCCAGGGCATTCTCGACGGTGAGCTATTCGACC
GGATGCGCCGCGGCGGCTACCTCATTCATGTCGGACGCGGCGAACATCTGGTGGACCAAGACCTGCTGGCGGCGCTGGAC
AGCGGCCAGCTCGCAGGCGCCGCACTCGACGTGTTCGCCGCGGAGCCGCTGCCGCCCACGCATCCGTTCTGGCGTCATCC
GAACATCGTCGTCACGCCGCACGACGCCAGCGACGTCAGCGTCGACTCCGTTGCGGCGACGCTGGTCGCAACCGCCAACG
CGATCCGAGCCGGGCGAAGGCCACCGCACGCGATCGACCGCGCACGCGGCTACTGA

Upstream 100 bases:

>100_bases
GCCGACCGCCGCGCTGTTTTCCGCTCCGGAGCATCCCTATACGCGCGAGCTGCTCGCCGCGGTGCCCGGCCAGACAGCCG
CTTCCAAGGCCGCCTGACCG

Downstream 100 bases:

>100_bases
TCTGACGCCCCGGATTGGCGGCGCCGCACGTTCACGCCAGCCAATGCGCGATGTCTTCGGCTTTCGGCAGCCCGCCGGCG
TGGACGACCTTGCCGTCGAT

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MRCALVSDRLDLRGFLGTDFARIADRIEFVDHRTDGANADIRLAVAWYPPDDAFAHYPNLRAVCSIGAGVDNITACPSLR
ADIDVVRIVDSAQAQMMSGFVLWHVIWHQRRFATYLAQQRDAVWQRIRQRNPREVPVAILGYGEIGQKVAADLAALGFPV
MVWSRSPKSTPAAIRGFHGREGLAAMLGETEVLVNLLPLTQETQGILDGELFDRMRRGGYLIHVGRGEHLVDQDLLAALD
SGQLAGAALDVFAAEPLPPTHPFWRHPNIVVTPHDASDVSVDSVAATLVATANAIRAGRRPPHAIDRARGY

Sequences:

>Translated_311_residues
MRCALVSDRLDLRGFLGTDFARIADRIEFVDHRTDGANADIRLAVAWYPPDDAFAHYPNLRAVCSIGAGVDNITACPSLR
ADIDVVRIVDSAQAQMMSGFVLWHVIWHQRRFATYLAQQRDAVWQRIRQRNPREVPVAILGYGEIGQKVAADLAALGFPV
MVWSRSPKSTPAAIRGFHGREGLAAMLGETEVLVNLLPLTQETQGILDGELFDRMRRGGYLIHVGRGEHLVDQDLLAALD
SGQLAGAALDVFAAEPLPPTHPFWRHPNIVVTPHDASDVSVDSVAATLVATANAIRAGRRPPHAIDRARGY
>Mature_311_residues
MRCALVSDRLDLRGFLGTDFARIADRIEFVDHRTDGANADIRLAVAWYPPDDAFAHYPNLRAVCSIGAGVDNITACPSLR
ADIDVVRIVDSAQAQMMSGFVLWHVIWHQRRFATYLAQQRDAVWQRIRQRNPREVPVAILGYGEIGQKVAADLAALGFPV
MVWSRSPKSTPAAIRGFHGREGLAAMLGETEVLVNLLPLTQETQGILDGELFDRMRRGGYLIHVGRGEHLVDQDLLAALD
SGQLAGAALDVFAAEPLPPTHPFWRHPNIVVTPHDASDVSVDSVAATLVATANAIRAGRRPPHAIDRARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=167, Percent_Identity=34.1317365269461, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI23308577, Length=292, Percent_Identity=27.0547945205479, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI61743967, Length=232, Percent_Identity=29.3103448275862, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4557497, Length=232, Percent_Identity=29.3103448275862, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI145580578, Length=232, Percent_Identity=26.7241379310345, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4557499, Length=232, Percent_Identity=26.7241379310345, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI145580575, Length=229, Percent_Identity=27.0742358078603, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI87081824, Length=281, Percent_Identity=39.5017793594306, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87082289, Length=226, Percent_Identity=26.9911504424779, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=219, Percent_Identity=26.9406392694064, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25147481, Length=229, Percent_Identity=24.8908296943231, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324964, Length=250, Percent_Identity=24.4, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324980, Length=144, Percent_Identity=30.5555555555556, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322116, Length=197, Percent_Identity=24.8730964467005, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320925, Length=197, Percent_Identity=24.8730964467005, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28574286, Length=246, Percent_Identity=26.0162601626016, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585514, Length=248, Percent_Identity=24.1935483870968, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574282, Length=248, Percent_Identity=24.1935483870968, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45552429, Length=248, Percent_Identity=24.1935483870968, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574284, Length=249, Percent_Identity=24.0963855421687, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45551003, Length=248, Percent_Identity=24.1935483870968, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585516, Length=253, Percent_Identity=26.4822134387352, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571528, Length=144, Percent_Identity=32.6388888888889, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34099; Mature: 34099

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCALVSDRLDLRGFLGTDFARIADRIEFVDHRTDGANADIRLAVAWYPPDDAFAHYPNL
CCCEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHCCCCH
RAVCSIGAGVDNITACPSLRADIDVVRIVDSAQAQMMSGFVLWHVIWHQRRFATYLAQQR
HHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAVWQRIRQRNPREVPVAILGYGEIGQKVAADLAALGFPVMVWSRSPKSTPAAIRGFHGR
HHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCCC
EGLAAMLGETEVLVNLLPLTQETQGILDGELFDRMRRGGYLIHVGRGEHLVDQDLLAALD
CHHHHHHCCHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHC
SGQLAGAALDVFAAEPLPPTHPFWRHPNIVVTPHDASDVSVDSVAATLVATANAIRAGRR
CCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PPHAIDRARGY
CCHHHHHCCCC
>Mature Secondary Structure
MRCALVSDRLDLRGFLGTDFARIADRIEFVDHRTDGANADIRLAVAWYPPDDAFAHYPNL
CCCEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHCCCCH
RAVCSIGAGVDNITACPSLRADIDVVRIVDSAQAQMMSGFVLWHVIWHQRRFATYLAQQR
HHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAVWQRIRQRNPREVPVAILGYGEIGQKVAADLAALGFPVMVWSRSPKSTPAAIRGFHGR
HHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCCC
EGLAAMLGETEVLVNLLPLTQETQGILDGELFDRMRRGGYLIHVGRGEHLVDQDLLAALD
CHHHHHHCCHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHC
SGQLAGAALDVFAAEPLPPTHPFWRHPNIVVTPHDASDVSVDSVAATLVATANAIRAGRR
CCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PPHAIDRARGY
CCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA