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The map label for this gene is bigR [H]

Identifier: 86748175

GI number: 86748175

Start: 1206971

End: 1207396

Strand: Direct

Name: bigR [H]

Synonym: RPB_1050

Alternate gene names: 86748175

Gene position: 1206971-1207396 (Clockwise)

Preceding gene: 86748173

Following gene: 86748178

Centisome position: 22.64

GC content: 68.08

Gene sequence:

>426_bases
ATGCCCGTGCCGAAGCGAGCCCGGAGACCATCGACCGATGGGCTTACGCATGTCGACACCGGCGAGCTGCGCGACAACGC
GTTGGTCGCCAGTGAATTCCTCAAGACGTTGTCGAACCCGTCGCGGCTGGTGATGCTGTGCCTGCTCGCCGAGGGCGAGA
GGTCGGTGTCGGAACTCGCAACCACGCTGGACGAGCGTCAGCCGACGGTGTCGCAGCAGCTCGCGCGGCTGCGCGGCGAG
CGGCTGGTGGAGACCCGCCGCGACGGGCAGCAGGTGTTCTATTCGCTCGCGAGCGATGAAGTCCGCAGCCTGATTCTCGT
GCTGCACGCGATCTTCTGCGCCAAGCCGCGCGGCAAGGCGGCCAAGGTCACGGTGCTGCGGCCGGTCAAGACGGTCGCCG
CGAAGAAGACCGCCGCGCGCCGATAG

Upstream 100 bases:

>100_bases
GGCTTGCTCAGGCGACTATGGGTTGCAACATCGGATCGATCGTCGGATTATCCTACGAGCGGTGAATTTCCAACTCCCTG
TTCCTGCAAGAGGCGAACCG

Downstream 100 bases:

>100_bases
CTCGGAGCGGAGCGGCGATCAGTCCGCCTTGGACCCGAACAGCCGCTTGAACAGGCCCGGCGGTTGCGGCCCCTTCACGG
TGTCGAAGCCGGCCTTGCGC

Product: ArsR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MPVPKRARRPSTDGLTHVDTGELRDNALVASEFLKTLSNPSRLVMLCLLAEGERSVSELATTLDERQPTVSQQLARLRGE
RLVETRRDGQQVFYSLASDEVRSLILVLHAIFCAKPRGKAAKVTVLRPVKTVAAKKTAARR

Sequences:

>Translated_141_residues
MPVPKRARRPSTDGLTHVDTGELRDNALVASEFLKTLSNPSRLVMLCLLAEGERSVSELATTLDERQPTVSQQLARLRGE
RLVETRRDGQQVFYSLASDEVRSLILVLHAIFCAKPRGKAAKVTVLRPVKTVAAKKTAARR
>Mature_140_residues
PVPKRARRPSTDGLTHVDTGELRDNALVASEFLKTLSNPSRLVMLCLLAEGERSVSELATTLDERQPTVSQQLARLRGER
LVETRRDGQQVFYSLASDEVRSLILVLHAIFCAKPRGKAAKVTVLRPVKTVAAKKTAARR

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=96, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 15603; Mature: 15472

Theoretical pI: Translated: 11.12; Mature: 11.12

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPKRARRPSTDGLTHVDTGELRDNALVASEFLKTLSNPSRLVMLCLLAEGERSVSELA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHH
TTLDERQPTVSQQLARLRGERLVETRRDGQQVFYSLASDEVRSLILVLHAIFCAKPRGKA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AKVTVLRPVKTVAAKKTAARR
EEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVPKRARRPSTDGLTHVDTGELRDNALVASEFLKTLSNPSRLVMLCLLAEGERSVSELA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHH
TTLDERQPTVSQQLARLRGERLVETRRDGQQVFYSLASDEVRSLILVLHAIFCAKPRGKA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AKVTVLRPVKTVAAKKTAARR
EEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]