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The map label for this gene is fabI1 [H]

Identifier: 86748150

GI number: 86748150

Start: 1176374

End: 1177135

Strand: Direct

Name: fabI1 [H]

Synonym: RPB_1025

Alternate gene names: 86748150

Gene position: 1176374-1177135 (Clockwise)

Preceding gene: 86748149

Following gene: 86748151

Centisome position: 22.06

GC content: 67.45

Gene sequence:

>762_bases
ATGACGCTGCAAGGTAAAAAGGGACTGGTGGTCGGGATCGCCAATGCGGAGAGCATCGCCTTCGGCTGCGCCCGGGCTTT
CCGCGACGCCGGCGCAGAGCTGGCCGTCACCTATCTCAACGACAAGGCGAAGCCGCATGTCGGGCCGCTCGCCGAACAGT
TGCAAAGCCCGATCGTGCTGCCCTGCGATGTCCGCGAGCCGGGCCAGCTCGAGGCGGTGTTCGCGCAGATCGGCGAGACC
TGGGGCCGGCTCGACTTCCTGCTGCATTCGATCGCCTTCGCCCCGAAGGAGGATCTGCACGGAAGGGTGGTGGATTGCTC
GCAGGCCGGCTTTGCGATGGCGATGGACGTGTCCTGCCATTCCTTCATCCGGATGGCGAAGCTCGCCGAGCCGCTGATGC
GGGACGGCGGCTGTCTGTTGACGGTCACGTTCTACGGCTCCGAGAAGGTGGTCGAGGACTACAATCTGATGGGGCCGGTG
AAGGCCGCGCTGGAAAGCTCGGTGCGCTACATGGCGGCCGAACTGGCGGTGAAGCGCATCCGAGTCCACGCGCTGTCGCC
GGGGCCGCTGAAGACCCGCGCCGCCTCCGGCATCGCCCGCTTCGACGAGTTGATGGAGCGCACCCGCGCCCGCGCCCCGG
CGCACCAGCTCGTCGGCATCGACGACGTCGGCCAGGTCGCGACCTTCCTGGTCGGCGACGGCGCCCGCGCGCTCACCGGC
AACATCGAATACATCGACGCCGGATATCACGTCGTCGGCTGA

Upstream 100 bases:

>100_bases
GGCTGCGACATCTCACCATTGATCGATGGTCGAAGCGCGCCGGTCACGCCAATGACACACCGGCCGGGTTAGGGAGTCGC
CGTAACGCGGTAGGGTGAGA

Downstream 100 bases:

>100_bases
CCGTCCGGACGCCTGGAGACATCATGGAGCAGATTCGAAATCGCACCTTCGACGAGATCGCGGTGGGCGACACCGCGAGC
CTGGTGCGGACGCTGACCTA

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTLQGKKGLVVGIANAESIAFGCARAFRDAGAELAVTYLNDKAKPHVGPLAEQLQSPIVLPCDVREPGQLEAVFAQIGET
WGRLDFLLHSIAFAPKEDLHGRVVDCSQAGFAMAMDVSCHSFIRMAKLAEPLMRDGGCLLTVTFYGSEKVVEDYNLMGPV
KAALESSVRYMAAELAVKRIRVHALSPGPLKTRAASGIARFDELMERTRARAPAHQLVGIDDVGQVATFLVGDGARALTG
NIEYIDAGYHVVG

Sequences:

>Translated_253_residues
MTLQGKKGLVVGIANAESIAFGCARAFRDAGAELAVTYLNDKAKPHVGPLAEQLQSPIVLPCDVREPGQLEAVFAQIGET
WGRLDFLLHSIAFAPKEDLHGRVVDCSQAGFAMAMDVSCHSFIRMAKLAEPLMRDGGCLLTVTFYGSEKVVEDYNLMGPV
KAALESSVRYMAAELAVKRIRVHALSPGPLKTRAASGIARFDELMERTRARAPAHQLVGIDDVGQVATFLVGDGARALTG
NIEYIDAGYHVVG
>Mature_252_residues
TLQGKKGLVVGIANAESIAFGCARAFRDAGAELAVTYLNDKAKPHVGPLAEQLQSPIVLPCDVREPGQLEAVFAQIGETW
GRLDFLLHSIAFAPKEDLHGRVVDCSQAGFAMAMDVSCHSFIRMAKLAEPLMRDGGCLLTVTFYGSEKVVEDYNLMGPVK
AALESSVRYMAAELAVKRIRVHALSPGPLKTRAASGIARFDELMERTRARAPAHQLVGIDDVGQVATFLVGDGARALTGN
IEYIDAGYHVVG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=250, Percent_Identity=42.4, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=27.3076923076923, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=26.984126984127, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=28.1746031746032, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27138; Mature: 27006

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQGKKGLVVGIANAESIAFGCARAFRDAGAELAVTYLNDKAKPHVGPLAEQLQSPIVL
CCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHCCCEEE
PCDVREPGQLEAVFAQIGETWGRLDFLLHSIAFAPKEDLHGRVVDCSQAGFAMAMDVSCH
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEHHHH
SFIRMAKLAEPLMRDGGCLLTVTFYGSEKVVEDYNLMGPVKAALESSVRYMAAELAVKRI
HHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RVHALSPGPLKTRAASGIARFDELMERTRARAPAHQLVGIDDVGQVATFLVGDGARALTG
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHCCCCCEEEEC
NIEYIDAGYHVVG
CEEEEECCEEECC
>Mature Secondary Structure 
TLQGKKGLVVGIANAESIAFGCARAFRDAGAELAVTYLNDKAKPHVGPLAEQLQSPIVL
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHCCCEEE
PCDVREPGQLEAVFAQIGETWGRLDFLLHSIAFAPKEDLHGRVVDCSQAGFAMAMDVSCH
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEHHHH
SFIRMAKLAEPLMRDGGCLLTVTFYGSEKVVEDYNLMGPVKAALESSVRYMAAELAVKRI
HHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RVHALSPGPLKTRAASGIARFDELMERTRARAPAHQLVGIDDVGQVATFLVGDGARALTG
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHCCCCCEEEEC
NIEYIDAGYHVVG
CEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]