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The map label for this gene is otsA [H]

Identifier: 86748146

GI number: 86748146

Start: 1170697

End: 1172913

Strand: Reverse

Name: otsA [H]

Synonym: RPB_1021

Alternate gene names: 86748146

Gene position: 1172913-1170697 (Counterclockwise)

Preceding gene: 86748148

Following gene: 86748140

Centisome position: 22.0

GC content: 66.44

Gene sequence:

>2217_bases
ATGCCGCGCTACATGGCAGCTCTGAAATATCTCGGCCTCGGCCTGCTGGTGATTCTGCTGCTCGGATTGGCGATCGCGCC
GTTTTCCAAACGCGTCGTCGAGCAATGGTCGCGCAGCGACGTCGAGGGCCGCTCGCGGCTGGTCTACAACGCGATCCAGG
GTCAGATCGTCGGCGCGATGGCCGACGACAACGTCGTGCAGCTCTCGGTGAGTTTCGAGGCGGTCGCGCTCGATCAGCGC
ATTCTCGCGGTCGGCCTGTGCAGCAGTCAGGGCCAGCTGATCGCGCCGACCCGGTTGATGCCGGCGACGTTTTCCTGCGA
GAAGGTGGCCCGCTCCGAGGGCGAGAGTTTCTCGACCATCGTGATCGATGGCCGCCGGGTGATGGTCGGCGCGTTCCCGA
TCGTCGATCGCGCGCGCAAATCCTATCTGGTCATCCTGCACGATCTCAGCTTCGTCGACGTCCGCTCCAGCGAGGCACAG
GGCTTCATGATCGCGGCGCTGGTCGGCGTGGCGCTGATGATCGCCGGCACCGCGGCGATCATCGTCGTGATGGTGCTGCG
CGGCTGGATGGCGGCGCTGCGGCGGGCGGTCGACGACGTGCGTTCCGGCGCGGCGCCGCCATTGCGGCAACGCGACCCGT
CGGCGATCGACCTGCAGATCAAGAAACTGCTGAGCGAAGTCGAAACCGGCCGCCATTCGATCAGTTCGGCGCAGATCGAC
TGGTCGCCGGCGACGCTGCAGCAATTGCTGCATTCGACCCTGCCGGACGCCGAAGTGCTGATCGTCTCCAACCGCGAGCC
CTACATCCACAATCGCGACGGCGACCGCACCGAGGTGCAGATCCCGGCGAGCGGCCTGGTGTCGGCGCTGGAACCTGTGA
TGCGCGCCTGCGGCGGCACCTGGATCGCGCACGGCAGCGGCAATGCCGACCGCGACACGGTGGATTCCCACGACCGCATC
GAGGTGCCGCCGGATCATCCGTCCTACAGGCTGCGCCGGATCTGGATCACCGACGAGGAACAGGACGGCTTCTATTACGG
CTTCGCCAATGAGGGCATGTGGCCGCTGTGCCATATCGCCTTCGTACGGCCGACGTTCCGGGAATCCGACTGGAAGGCCT
ATCAGCGGATCAACGAGCGCTTCGCCGCCGCCGTCGTCGAGGAGGCGAAGACCGACAACCCGATCGTGCTGGTGCAGGAC
TATCACTTCGCGCTGGCGCCGCGGATGATCCGCGATCGTCTACCGAAGGCGACCATCATCACCTTCTGGCACATCCCGTG
GCCGAACGCCGAGACCTTCAGCATCTGTCCGTGGAAGGAGCAGATCATCGACGGCCTGCTCGGCTCCACCATCCTCGGCT
TCCACACCCAGTTCCACTGCAACAATTTCTTCGAGACCGTCGATCGCTTCGTCGAGAGCCGGATCGACCGCGAGCACGCC
ACCGTGACGCTGTCGGGCCACGAAACCATGGTGCGGGCCTATCCGATCTCGATCGAATGGCCGCCGGCGGCGCTGGACGG
CCAGCCGCCGGTCGAAACCTGCCGCCGCGAGGTGCGCGAGGCGCTCGGGCTCGCCGCCGACGTCAGGATCGCGGTCGGCA
TCGAGCGCTTCGACTACACCAAGGGGATTCTCGACCGCATGAAGGCGGTCGACGATCTGCTGACGCGGCAGCCGCAATGG
AAACGCCAGCTGGTGTTCGTCCAGGTCGCGGCGCCGACGCGCAGCAAATTGTCGAGCTACAGCACGCTGCAGGACGACGC
GGTGGCGCTCGCCGACGACATCAACCGGCGGCACGGATCGGACGGCTACAAGCCGATCGTGCTGCTGATCCGGCATCACA
GCGCGCGCGAGGTGTTCAAGCTGTTCCGCGCCGCCGACGTCTGCATCGTCAGCAGCCTGCACGACGGCATGAACCTCGTC
GCCAAGGAATTCGCCGCCGCCCGCGACGACGAGCGCGGCGTGCTGGTGCTGTCGAGCTTCACCGGCGCCTCGCGCGAACT
GTCCGAGGCGCTGATCGTCAATCCGTATCACGTCCACGAAACCGCGACCGCGCTCGACACCGCGCTGCGGATGCCGGAGC
ACGAACAGCAGGAGCGGATGCGCGCGATGCGTCAGCAGATCCGCGAGTGGAACGTGTATCGCTGGGCCGGCCGGATGCTG
ATCGACGCCGCCACCAGCCGCCGCCGCCAGCGCATTCTCGATCTCGCCGAGGGCTGA

Upstream 100 bases:

>100_bases
ACTGGAACCCGGATGCTGCGCTCGATGTGAAGCATTCACGACTGACGCGACCGACCGCGTCGTGTAAACAGCAGCGGGTC
GCATGGGGGAACTTCGGTCG

Downstream 100 bases:

>100_bases
GCGGACCGCGGCGCTACAGCTTGAACGGGCCGAGCGGGCCGTGTTCGGCCATGTCGACGAACGCCCAATACCGCGCGCGC
ACGTCGGCCAGATCGTCCGG

Product: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MPRYMAALKYLGLGLLVILLLGLAIAPFSKRVVEQWSRSDVEGRSRLVYNAIQGQIVGAMADDNVVQLSVSFEAVALDQR
ILAVGLCSSQGQLIAPTRLMPATFSCEKVARSEGESFSTIVIDGRRVMVGAFPIVDRARKSYLVILHDLSFVDVRSSEAQ
GFMIAALVGVALMIAGTAAIIVVMVLRGWMAALRRAVDDVRSGAAPPLRQRDPSAIDLQIKKLLSEVETGRHSISSAQID
WSPATLQQLLHSTLPDAEVLIVSNREPYIHNRDGDRTEVQIPASGLVSALEPVMRACGGTWIAHGSGNADRDTVDSHDRI
EVPPDHPSYRLRRIWITDEEQDGFYYGFANEGMWPLCHIAFVRPTFRESDWKAYQRINERFAAAVVEEAKTDNPIVLVQD
YHFALAPRMIRDRLPKATIITFWHIPWPNAETFSICPWKEQIIDGLLGSTILGFHTQFHCNNFFETVDRFVESRIDREHA
TVTLSGHETMVRAYPISIEWPPAALDGQPPVETCRREVREALGLAADVRIAVGIERFDYTKGILDRMKAVDDLLTRQPQW
KRQLVFVQVAAPTRSKLSSYSTLQDDAVALADDINRRHGSDGYKPIVLLIRHHSAREVFKLFRAADVCIVSSLHDGMNLV
AKEFAAARDDERGVLVLSSFTGASRELSEALIVNPYHVHETATALDTALRMPEHEQQERMRAMRQQIREWNVYRWAGRML
IDAATSRRRQRILDLAEG

Sequences:

>Translated_738_residues
MPRYMAALKYLGLGLLVILLLGLAIAPFSKRVVEQWSRSDVEGRSRLVYNAIQGQIVGAMADDNVVQLSVSFEAVALDQR
ILAVGLCSSQGQLIAPTRLMPATFSCEKVARSEGESFSTIVIDGRRVMVGAFPIVDRARKSYLVILHDLSFVDVRSSEAQ
GFMIAALVGVALMIAGTAAIIVVMVLRGWMAALRRAVDDVRSGAAPPLRQRDPSAIDLQIKKLLSEVETGRHSISSAQID
WSPATLQQLLHSTLPDAEVLIVSNREPYIHNRDGDRTEVQIPASGLVSALEPVMRACGGTWIAHGSGNADRDTVDSHDRI
EVPPDHPSYRLRRIWITDEEQDGFYYGFANEGMWPLCHIAFVRPTFRESDWKAYQRINERFAAAVVEEAKTDNPIVLVQD
YHFALAPRMIRDRLPKATIITFWHIPWPNAETFSICPWKEQIIDGLLGSTILGFHTQFHCNNFFETVDRFVESRIDREHA
TVTLSGHETMVRAYPISIEWPPAALDGQPPVETCRREVREALGLAADVRIAVGIERFDYTKGILDRMKAVDDLLTRQPQW
KRQLVFVQVAAPTRSKLSSYSTLQDDAVALADDINRRHGSDGYKPIVLLIRHHSAREVFKLFRAADVCIVSSLHDGMNLV
AKEFAAARDDERGVLVLSSFTGASRELSEALIVNPYHVHETATALDTALRMPEHEQQERMRAMRQQIREWNVYRWAGRML
IDAATSRRRQRILDLAEG
>Mature_737_residues
PRYMAALKYLGLGLLVILLLGLAIAPFSKRVVEQWSRSDVEGRSRLVYNAIQGQIVGAMADDNVVQLSVSFEAVALDQRI
LAVGLCSSQGQLIAPTRLMPATFSCEKVARSEGESFSTIVIDGRRVMVGAFPIVDRARKSYLVILHDLSFVDVRSSEAQG
FMIAALVGVALMIAGTAAIIVVMVLRGWMAALRRAVDDVRSGAAPPLRQRDPSAIDLQIKKLLSEVETGRHSISSAQIDW
SPATLQQLLHSTLPDAEVLIVSNREPYIHNRDGDRTEVQIPASGLVSALEPVMRACGGTWIAHGSGNADRDTVDSHDRIE
VPPDHPSYRLRRIWITDEEQDGFYYGFANEGMWPLCHIAFVRPTFRESDWKAYQRINERFAAAVVEEAKTDNPIVLVQDY
HFALAPRMIRDRLPKATIITFWHIPWPNAETFSICPWKEQIIDGLLGSTILGFHTQFHCNNFFETVDRFVESRIDREHAT
VTLSGHETMVRAYPISIEWPPAALDGQPPVETCRREVREALGLAADVRIAVGIERFDYTKGILDRMKAVDDLLTRQPQWK
RQLVFVQVAAPTRSKLSSYSTLQDDAVALADDINRRHGSDGYKPIVLLIRHHSAREVFKLFRAADVCIVSSLHDGMNLVA
KEFAAARDDERGVLVLSSFTGASRELSEALIVNPYHVHETATALDTALRMPEHEQQERMRAMRQQIREWNVYRWAGRMLI
DAATSRRRQRILDLAEG

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=481, Percent_Identity=29.1060291060291, Blast_Score=199, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI115533558, Length=366, Percent_Identity=28.1420765027322, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115533560, Length=366, Percent_Identity=28.1420765027322, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71987012, Length=425, Percent_Identity=24.9411764705882, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319602, Length=469, Percent_Identity=29.2110874200426, Blast_Score=233, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6323917, Length=388, Percent_Identity=25.2577319587629, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6323537, Length=389, Percent_Identity=26.4781491002571, Blast_Score=151, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320279, Length=419, Percent_Identity=24.1050119331742, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19920676, Length=488, Percent_Identity=32.7868852459016, Blast_Score=254, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 82766; Mature: 82635

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYMAALKYLGLGLLVILLLGLAIAPFSKRVVEQWSRSDVEGRSRLVYNAIQGQIVGAM
CCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEE
ADDNVVQLSVSFEAVALDQRILAVGLCSSQGQLIAPTRLMPATFSCEKVARSEGESFSTI
CCCCEEEEEEEEEEHHHHHHEEEEEEECCCCCEECCCCCCCCCCCHHHHHHCCCCCEEEE
VIDGRRVMVGAFPIVDRARKSYLVILHDLSFVDVRSSEAQGFMIAALVGVALMIAGTAAI
EEECCEEEEECCHHHHHCCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
IVVMVLRGWMAALRRAVDDVRSGAAPPLRQRDPSAIDLQIKKLLSEVETGRHSISSAQID
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEC
WSPATLQQLLHSTLPDAEVLIVSNREPYIHNRDGDRTEVQIPASGLVSALEPVMRACGGT
CCHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCEEEEECHHHHHHHHHHHHHHCCCC
WIAHGSGNADRDTVDSHDRIEVPPDHPSYRLRRIWITDEEQDGFYYGFANEGMWPLCHIA
EEEECCCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHH
FVRPTFRESDWKAYQRINERFAAAVVEEAKTDNPIVLVQDYHFALAPRMIRDRLPKATII
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCEEEE
TFWHIPWPNAETFSICPWKEQIIDGLLGSTILGFHTQFHCNNFFETVDRFVESRIDREHA
EEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TVTLSGHETMVRAYPISIEWPPAALDGQPPVETCRREVREALGLAADVRIAVGIERFDYT
EEEECCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHCEEEEEEHHHHHHH
KGILDRMKAVDDLLTRQPQWKRQLVFVQVAAPTRSKLSSYSTLQDDAVALADDINRRHGS
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHCCC
DGYKPIVLLIRHHSAREVFKLFRAADVCIVSSLHDGMNLVAKEFAAARDDERGVLVLSSF
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGASRELSEALIVNPYHVHETATALDTALRMPEHEQQERMRAMRQQIREWNVYRWAGRML
CCCCHHHHHHHEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
IDAATSRRRQRILDLAEG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRYMAALKYLGLGLLVILLLGLAIAPFSKRVVEQWSRSDVEGRSRLVYNAIQGQIVGAM
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEE
ADDNVVQLSVSFEAVALDQRILAVGLCSSQGQLIAPTRLMPATFSCEKVARSEGESFSTI
CCCCEEEEEEEEEEHHHHHHEEEEEEECCCCCEECCCCCCCCCCCHHHHHHCCCCCEEEE
VIDGRRVMVGAFPIVDRARKSYLVILHDLSFVDVRSSEAQGFMIAALVGVALMIAGTAAI
EEECCEEEEECCHHHHHCCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
IVVMVLRGWMAALRRAVDDVRSGAAPPLRQRDPSAIDLQIKKLLSEVETGRHSISSAQID
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEC
WSPATLQQLLHSTLPDAEVLIVSNREPYIHNRDGDRTEVQIPASGLVSALEPVMRACGGT
CCHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCEEEEECHHHHHHHHHHHHHHCCCC
WIAHGSGNADRDTVDSHDRIEVPPDHPSYRLRRIWITDEEQDGFYYGFANEGMWPLCHIA
EEEECCCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHH
FVRPTFRESDWKAYQRINERFAAAVVEEAKTDNPIVLVQDYHFALAPRMIRDRLPKATII
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCEEEE
TFWHIPWPNAETFSICPWKEQIIDGLLGSTILGFHTQFHCNNFFETVDRFVESRIDREHA
EEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TVTLSGHETMVRAYPISIEWPPAALDGQPPVETCRREVREALGLAADVRIAVGIERFDYT
EEEECCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHCEEEEEEHHHHHHH
KGILDRMKAVDDLLTRQPQWKRQLVFVQVAAPTRSKLSSYSTLQDDAVALADDINRRHGS
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHCCC
DGYKPIVLLIRHHSAREVFKLFRAADVCIVSSLHDGMNLVAKEFAAARDDERGVLVLSSF
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGASRELSEALIVNPYHVHETATALDTALRMPEHEQQERMRAMRQQIREWNVYRWAGRML
CCCCHHHHHHHEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
IDAATSRRRQRILDLAEG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA