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The map label for this gene is nifA [H]

Identifier: 86748082

GI number: 86748082

Start: 1106632

End: 1108383

Strand: Direct

Name: nifA [H]

Synonym: RPB_0957

Alternate gene names: 86748082

Gene position: 1106632-1108383 (Clockwise)

Preceding gene: 86748081

Following gene: 86748083

Centisome position: 20.76

GC content: 64.44

Gene sequence:

>1752_bases
ATGGCTCAGCGCGAAGTACGTCTTGTCGAGAGCGAGCAATCGCGGCAGCCGATGAACCAGAACCCGATACCGCTGAGTGA
GATTGCGCTCACCGGCATTTTTGAAATCTCCAAGATCCTCACAGCGCCGGCGCGGCTCGAAGTCACGCTCGCGAATGTCG
TCAATCTGCTGCAGTCCTTTCTGCAGATGCGAAATGGCGTCGTGTCGCTGCTGGCCGACGACAGTGTTCCCGACATCACG
GTCGGCGTGGGCTGGAACGAAGGCAGCGACAATCGCTATCGCGCGCGACTCCCGCAGAAAGCCATCGACCAGATCGTCGC
CACCTCGGTGCCGCTGGTCGCCGACAACGTGGCCGCGCATCCGATGTTCTCCGCCGCCGACGCGCTCGCGCTGGGCGCGA
CCGACGAGACCCGTGTGTCGTTCATCGGCGTGCCGATCCGGATCGATTCGCGGGTCGTGGGCACCCTGACGATCGACCGG
GTTCGCGACGGGCAGTCGATCTTCCGGATGGACGCCGATGTCCGGTTCCTGACTATGGTCGCCAATCTGATCGGGCAGAC
CGTGAAGCTGCATCGCGTGGTGGCGCGTGATCGCGAGCGGCTGATGGCGGAAAGCCATCGCCTGCAGAAAGAGCTGTACG
AGTTGAAGCCGCAGCGCGAGCGCAAGCGCGTCCGGGTCGACGGCATCGTCGGCGAGAGCCCGGCGATCCGCACGTTGCTC
GCCAAGGTCAGCATCATCGCCAAATCGCAGTCGCCGGTGCTTTTGCGCGGCGAGTCCGGCACCGGCAAGGAACTGATCGC
CAAGGCGATCCACGAATTGTCGGCGCGCGCCAACGGGCCCTTCATCAAGATCAACTGCGCCGCGCTGCCGGAGTCGGTGT
TGGAATCCGAACTGTTCGGCCACGAGAAGGGGGCTTTCACCGGCGCGATCGCTTCACGCAAGGGCCGGTTCGAACTCGCC
GACAAGGGCACGCTGTTTCTCGACGAGATCGGCGAGATCTCGGCCTCGTTCCAGGCCAAGCTGCTGCGCGTGCTGCAGGA
GCAGGAGTTCGAGCGGGTCGGCGGCAACCAGACCATCAAGGTCAACGTCCGAATCGTCGCGGCGACCAACCGCAATCTCG
AAGAGGCCGTCGCCCGCAAGGAGTTCCGCGCCGATCTGTACTACCGCATCAACGTCGTGCCGATGATCCTGCCGCCGCTG
CGCGATAGGCCGACCGATATCCCATTGCTGGCGAGCGAGTTCCTGAAGAACTTCAACAAGGAGAACGATCGCGAACTGCA
ATTCGAGCCGCATGCGCTGGAATTGCTGAAGGCGTGCTCGTTCCCGGGCAACGTTCGCGAACTCGAGAACTGCGTGCGGC
GCACGGCGACGTTGGCGATCGGGCCGGAAATTACCGACAGCGATTTCGCCTGCCATCAGGACGAATGCCTGTCGGCGATC
TTGTGGAAGGGCCACGCCGAACCGGCACCGGTACGGCCGCGGCCGCAGATTCCGCTGCAGGTGATGCCGCGCAAGGCGCC
GCTCGAAGTCGTGGCGCCGCGCGAGGCCGTGAGTGTGTCGCCCGATCCGGTGTCGACACCCATGTCCGCCGAATCGGCCA
ACGGCGGGCCGATGTCGGAGCGCGAGCGCTTGGTCAACGCGATGGAGCGATCCGGCTGGGTCCAGGCAAAGGCCGCCCGG
CTGCTCGGCCTGACGCCGCGGCAGATCGGCTACGCGCTGAAGAAGTACGATATCGAGCTCAAACACTTCTGA

Upstream 100 bases:

>100_bases
TGATGCAGCCAAGTTGAGCACGACATCTGATTGAGGATCATACACAATCTCGTAATGCTCCGGAACTAAGGGGGATGTCT
CAGGAGCGATGGAGATAGCA

Downstream 100 bases:

>100_bases
CAGCGTCTCCGCGGGAAGTTGTTACTGGTTCGCATTGACAATCGCATCTGCTCATCGAGAGCTTCGGTCCTGATCCGCGA
TCAGACCGAAGCTCTCGTCG

Product: transcriptional regulator NifA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MAQREVRLVESEQSRQPMNQNPIPLSEIALTGIFEISKILTAPARLEVTLANVVNLLQSFLQMRNGVVSLLADDSVPDIT
VGVGWNEGSDNRYRARLPQKAIDQIVATSVPLVADNVAAHPMFSAADALALGATDETRVSFIGVPIRIDSRVVGTLTIDR
VRDGQSIFRMDADVRFLTMVANLIGQTVKLHRVVARDRERLMAESHRLQKELYELKPQRERKRVRVDGIVGESPAIRTLL
AKVSIIAKSQSPVLLRGESGTGKELIAKAIHELSARANGPFIKINCAALPESVLESELFGHEKGAFTGAIASRKGRFELA
DKGTLFLDEIGEISASFQAKLLRVLQEQEFERVGGNQTIKVNVRIVAATNRNLEEAVARKEFRADLYYRINVVPMILPPL
RDRPTDIPLLASEFLKNFNKENDRELQFEPHALELLKACSFPGNVRELENCVRRTATLAIGPEITDSDFACHQDECLSAI
LWKGHAEPAPVRPRPQIPLQVMPRKAPLEVVAPREAVSVSPDPVSTPMSAESANGGPMSERERLVNAMERSGWVQAKAAR
LLGLTPRQIGYALKKYDIELKHF

Sequences:

>Translated_583_residues
MAQREVRLVESEQSRQPMNQNPIPLSEIALTGIFEISKILTAPARLEVTLANVVNLLQSFLQMRNGVVSLLADDSVPDIT
VGVGWNEGSDNRYRARLPQKAIDQIVATSVPLVADNVAAHPMFSAADALALGATDETRVSFIGVPIRIDSRVVGTLTIDR
VRDGQSIFRMDADVRFLTMVANLIGQTVKLHRVVARDRERLMAESHRLQKELYELKPQRERKRVRVDGIVGESPAIRTLL
AKVSIIAKSQSPVLLRGESGTGKELIAKAIHELSARANGPFIKINCAALPESVLESELFGHEKGAFTGAIASRKGRFELA
DKGTLFLDEIGEISASFQAKLLRVLQEQEFERVGGNQTIKVNVRIVAATNRNLEEAVARKEFRADLYYRINVVPMILPPL
RDRPTDIPLLASEFLKNFNKENDRELQFEPHALELLKACSFPGNVRELENCVRRTATLAIGPEITDSDFACHQDECLSAI
LWKGHAEPAPVRPRPQIPLQVMPRKAPLEVVAPREAVSVSPDPVSTPMSAESANGGPMSERERLVNAMERSGWVQAKAAR
LLGLTPRQIGYALKKYDIELKHF
>Mature_582_residues
AQREVRLVESEQSRQPMNQNPIPLSEIALTGIFEISKILTAPARLEVTLANVVNLLQSFLQMRNGVVSLLADDSVPDITV
GVGWNEGSDNRYRARLPQKAIDQIVATSVPLVADNVAAHPMFSAADALALGATDETRVSFIGVPIRIDSRVVGTLTIDRV
RDGQSIFRMDADVRFLTMVANLIGQTVKLHRVVARDRERLMAESHRLQKELYELKPQRERKRVRVDGIVGESPAIRTLLA
KVSIIAKSQSPVLLRGESGTGKELIAKAIHELSARANGPFIKINCAALPESVLESELFGHEKGAFTGAIASRKGRFELAD
KGTLFLDEIGEISASFQAKLLRVLQEQEFERVGGNQTIKVNVRIVAATNRNLEEAVARKEFRADLYYRINVVPMILPPLR
DRPTDIPLLASEFLKNFNKENDRELQFEPHALELLKACSFPGNVRELENCVRRTATLAIGPEITDSDFACHQDECLSAIL
WKGHAEPAPVRPRPQIPLQVMPRKAPLEVVAPREAVSVSPDPVSTPMSAESANGGPMSERERLVNAMERSGWVQAKAARL
LGLTPRQIGYALKKYDIELKHF

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=242, Percent_Identity=59.504132231405, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI87082117, Length=265, Percent_Identity=53.5849056603774, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1789087, Length=292, Percent_Identity=50.6849315068493, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI1790437, Length=345, Percent_Identity=43.768115942029, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI87082152, Length=237, Percent_Identity=53.1645569620253, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1790299, Length=267, Percent_Identity=46.4419475655431, Blast_Score=241, Evalue=8e-65,
Organism=Escherichia coli, GI1788905, Length=242, Percent_Identity=49.5867768595041, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789233, Length=417, Percent_Identity=35.9712230215827, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI87081872, Length=354, Percent_Identity=40.1129943502825, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=253, Percent_Identity=44.2687747035573, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1787583, Length=241, Percent_Identity=41.9087136929461, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789828, Length=249, Percent_Identity=38.5542168674699, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87081858, Length=352, Percent_Identity=28.125, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 64538; Mature: 64407

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQREVRLVESEQSRQPMNQNPIPLSEIALTGIFEISKILTAPARLEVTLANVVNLLQSF
CCCCHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
LQMRNGVVSLLADDSVPDITVGVGWNEGSDNRYRARLPQKAIDQIVATSVPLVADNVAAH
HHHHCCEEEEEECCCCCCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHCCCHHCCCCCCC
PMFSAADALALGATDETRVSFIGVPIRIDSRVVGTLTIDRVRDGQSIFRMDADVRFLTMV
CCHHHCCHHEECCCCCCEEEEEEEEEEECCEEEEEEEEEHHCCCCHHEEECCCHHHHHHH
ANLIGQTVKLHRVVARDRERLMAESHRLQKELYELKPQRERKRVRVDGIVGESPAIRTLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEECCCCCCCHHHHHHH
AKVSIIAKSQSPVLLRGESGTGKELIAKAIHELSARANGPFIKINCAALPESVLESELFG
HHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHC
HEKGAFTGAIASRKGRFELADKGTLFLDEIGEISASFQAKLLRVLQEQEFERVGGNQTIK
CCCCCEEHHHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VNVRIVAATNRNLEEAVARKEFRADLYYRINVVPMILPPLRDRPTDIPLLASEFLKNFNK
EEEEEEEECCCCHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCC
ENDRELQFEPHALELLKACSFPGNVRELENCVRRTATLAIGPEITDSDFACHQDECLSAI
CCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHH
LWKGHAEPAPVRPRPQIPLQVMPRKAPLEVVAPREAVSVSPDPVSTPMSAESANGGPMSE
HHCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCHHH
RERLVNAMERSGWVQAKAARLLGLTPRQIGYALKKYDIELKHF
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCEEECCC
>Mature Secondary Structure 
AQREVRLVESEQSRQPMNQNPIPLSEIALTGIFEISKILTAPARLEVTLANVVNLLQSF
CCCHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
LQMRNGVVSLLADDSVPDITVGVGWNEGSDNRYRARLPQKAIDQIVATSVPLVADNVAAH
HHHHCCEEEEEECCCCCCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHCCCHHCCCCCCC
PMFSAADALALGATDETRVSFIGVPIRIDSRVVGTLTIDRVRDGQSIFRMDADVRFLTMV
CCHHHCCHHEECCCCCCEEEEEEEEEEECCEEEEEEEEEHHCCCCHHEEECCCHHHHHHH
ANLIGQTVKLHRVVARDRERLMAESHRLQKELYELKPQRERKRVRVDGIVGESPAIRTLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEECCCCCCCHHHHHHH
AKVSIIAKSQSPVLLRGESGTGKELIAKAIHELSARANGPFIKINCAALPESVLESELFG
HHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHC
HEKGAFTGAIASRKGRFELADKGTLFLDEIGEISASFQAKLLRVLQEQEFERVGGNQTIK
CCCCCEEHHHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VNVRIVAATNRNLEEAVARKEFRADLYYRINVVPMILPPLRDRPTDIPLLASEFLKNFNK
EEEEEEEECCCCHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCC
ENDRELQFEPHALELLKACSFPGNVRELENCVRRTATLAIGPEITDSDFACHQDECLSAI
CCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHH
LWKGHAEPAPVRPRPQIPLQVMPRKAPLEVVAPREAVSVSPDPVSTPMSAESANGGPMSE
HHCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCHHH
RERLVNAMERSGWVQAKAARLLGLTPRQIGYALKKYDIELKHF
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3313281; 12597275; 3357773; 2792368 [H]