Click here to switch to the map view.

The map label for this gene is livM [H]

Identifier: 86748068

GI number: 86748068

Start: 1090998

End: 1092053

Strand: Direct

Name: livM [H]

Synonym: RPB_0943

Alternate gene names: 86748068

Gene position: 1090998-1092053 (Clockwise)

Preceding gene: 86748067

Following gene: 86748069

Centisome position: 20.46

GC content: 63.26

Gene sequence:

>1056_bases
ATGACGGATACTTCTTCGACGGCTGCGCAGTTCACCGCGCCTCGCACCGGGCGCCGCCTGAAGGTGCTGCCGATTATCGA
GGTGGTCGTTCTCGCGATCGGGCTGCTGTTGCCGCTGGTGCTGCAGGATTATCTCACGGTGTTCGGCACGCGGGTGCTAA
TCCTGTGCCTGTTCGCGCTGTCGTTCGATCTGGTGTGGGGCTATTCCGGCATCATGAGCTTCGGCCAGGCGGTGTTCTTC
GGCTCGGCCGGCTACGGCGTCGCGTTGCTGGCACGCGATCTCAACGTCACCTCGATGCTGCTGGTGCTTCCAGCCGGCGT
GCTGATCGGATTCAGCATCGCGCTGCTGATCGGCGGCTTCCTGCTGCTCGGGCGCAATCCGTCGAGCGTCATCTTCGTCT
CGCTGGGTACGCTCACCGGCTCCTATGCGTTCGATCGGCTGGCGCGTGGCTGGTACTATCTCGGCGGCCAGAACGGCATT
CCCTCGATCCCGCCGATGCAGATCGGCGGCTACGATCTCACCGAAGGGCCGGTGTTTTACTATTTCGTGCTGGCGATCCT
GCTGCTGGTCTATCTCGGCTGCCGCTTCCTGACCAAGTCGCAATTCGGGCTCGCGCTCGCGGGCCTGCGCGAGAACGAGC
AGCGCATCGCCTTTTTCGGCTACCAGGTGCAACACATCAAGGCGATCATCTTCTCGCTCGCCGGCGCGATCGCGGGCCTC
GCGGGCAGCCTTTACGCCTTCCACGAAGGCTTCGTCTGGCCGAACATGCTCGGCGTTGTGATGTCGACCCAGGTGGTGCT
GTATGTATTGTTCGGCGGCTCCGGCACGCTGATCGGCGCGGTGATCGGCACGGTCGTCATCGAGATCGTGTCGTTCTGGC
TGTCCAACTCCTACCAGGACATCTGGCCGATCATCCTCGGCTGCCTGATGCTGCTGGTGATCCTGTTCCGTCCGGCCGGG
CTGATCAGCCTGATCGTCAGCCACAGCGAACGGATCGGCAATTTCGGCCGGCCGCCGAAACCGCACCGGAAGGAGCGCGA
TCATGGCTCTGCTTGA

Upstream 100 bases:

>100_bases
TGCGTGCGGCAGCGCTCTGCTTATTTACGACGACGATGCCCGCGGACCATGCGGCGCTCTCCTCGCACGCGTCACCCCCA
TTCAGCACCCTGGCGTATCG

Downstream 100 bases:

>100_bases
AGCGCGCGGCCTGAAGAAGATCTTCGGCAAGCTCACCGCGTTGAATGGCGCCGAGCTGATCGTGCAGCCCAACGAGTTCC
ACGGCCTGATCGGTCCAAAC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTDTSSTAAQFTAPRTGRRLKVLPIIEVVVLAIGLLLPLVLQDYLTVFGTRVLILCLFALSFDLVWGYSGIMSFGQAVFF
GSAGYGVALLARDLNVTSMLLVLPAGVLIGFSIALLIGGFLLLGRNPSSVIFVSLGTLTGSYAFDRLARGWYYLGGQNGI
PSIPPMQIGGYDLTEGPVFYYFVLAILLLVYLGCRFLTKSQFGLALAGLRENEQRIAFFGYQVQHIKAIIFSLAGAIAGL
AGSLYAFHEGFVWPNMLGVVMSTQVVLYVLFGGSGTLIGAVIGTVVIEIVSFWLSNSYQDIWPIILGCLMLLVILFRPAG
LISLIVSHSERIGNFGRPPKPHRKERDHGSA

Sequences:

>Translated_351_residues
MTDTSSTAAQFTAPRTGRRLKVLPIIEVVVLAIGLLLPLVLQDYLTVFGTRVLILCLFALSFDLVWGYSGIMSFGQAVFF
GSAGYGVALLARDLNVTSMLLVLPAGVLIGFSIALLIGGFLLLGRNPSSVIFVSLGTLTGSYAFDRLARGWYYLGGQNGI
PSIPPMQIGGYDLTEGPVFYYFVLAILLLVYLGCRFLTKSQFGLALAGLRENEQRIAFFGYQVQHIKAIIFSLAGAIAGL
AGSLYAFHEGFVWPNMLGVVMSTQVVLYVLFGGSGTLIGAVIGTVVIEIVSFWLSNSYQDIWPIILGCLMLLVILFRPAG
LISLIVSHSERIGNFGRPPKPHRKERDHGSA
>Mature_350_residues
TDTSSTAAQFTAPRTGRRLKVLPIIEVVVLAIGLLLPLVLQDYLTVFGTRVLILCLFALSFDLVWGYSGIMSFGQAVFFG
SAGYGVALLARDLNVTSMLLVLPAGVLIGFSIALLIGGFLLLGRNPSSVIFVSLGTLTGSYAFDRLARGWYYLGGQNGIP
SIPPMQIGGYDLTEGPVFYYFVLAILLLVYLGCRFLTKSQFGLALAGLRENEQRIAFFGYQVQHIKAIIFSLAGAIAGLA
GSLYAFHEGFVWPNMLGVVMSTQVVLYVLFGGSGTLIGAVIGTVVIEIVSFWLSNSYQDIWPIILGCLMLLVILFRPAGL
ISLIVSHSERIGNFGRPPKPHRKERDHGSA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=305, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 37955; Mature: 37823

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTSSTAAQFTAPRTGRRLKVLPIIEVVVLAIGLLLPLVLQDYLTVFGTRVLILCLFAL
CCCCCCCHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFDLVWGYSGIMSFGQAVFFGSAGYGVALLARDLNVTSMLLVLPAGVLIGFSIALLIGGF
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLLGRNPSSVIFVSLGTLTGSYAFDRLARGWYYLGGQNGIPSIPPMQIGGYDLTEGPVFY
HHCCCCCCCEEEEEECCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
YFVLAILLLVYLGCRFLTKSQFGLALAGLRENEQRIAFFGYQVQHIKAIIFSLAGAIAGL
HHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
AGSLYAFHEGFVWPNMLGVVMSTQVVLYVLFGGSGTLIGAVIGTVVIEIVSFWLSNSYQD
HHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHH
IWPIILGCLMLLVILFRPAGLISLIVSHSERIGNFGRPPKPHRKERDHGSA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDTSSTAAQFTAPRTGRRLKVLPIIEVVVLAIGLLLPLVLQDYLTVFGTRVLILCLFAL
CCCCCCHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFDLVWGYSGIMSFGQAVFFGSAGYGVALLARDLNVTSMLLVLPAGVLIGFSIALLIGGF
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLLGRNPSSVIFVSLGTLTGSYAFDRLARGWYYLGGQNGIPSIPPMQIGGYDLTEGPVFY
HHCCCCCCCEEEEEECCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
YFVLAILLLVYLGCRFLTKSQFGLALAGLRENEQRIAFFGYQVQHIKAIIFSLAGAIAGL
HHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
AGSLYAFHEGFVWPNMLGVVMSTQVVLYVLFGGSGTLIGAVIGTVVIEIVSFWLSNSYQD
HHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHH
IWPIILGCLMLLVILFRPAGLISLIVSHSERIGNFGRPPKPHRKERDHGSA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]