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The map label for this gene is irgB [H]

Identifier: 86748062

GI number: 86748062

Start: 1084050

End: 1085048

Strand: Direct

Name: irgB [H]

Synonym: RPB_0937

Alternate gene names: 86748062

Gene position: 1084050-1085048 (Clockwise)

Preceding gene: 86748061

Following gene: 86748064

Centisome position: 20.33

GC content: 61.16

Gene sequence:

>999_bases
ATGACGGTCGCGCGATCGGCGCAGCAGCGCCGGTCGCCCCGCAGGACGGCAGAGCACGTGGCCAAGACACAACGCAAGAT
CCGCTATGACTGGAACGATGTCGTCTACTTTCTCGAGGTGGCCCGGCAACGCAGCCTGGTGCGCGCCGCGGCCAAGCTGA
AGGTCGACCACACCACCGTCAGCCGGCGCATCCGGGAGCTCGAGCGAAGCCTGAATTCGACGCTGTTCAAGCGCAGCAAA
TCCGGCTTCAGCCTGACCGAAATAGGCATGCGGCTACTGCAATATGCCGAGGGCATGGAAAGCCAGGCGAACGCGATCGT
CGAGGCGATCGGCACCGACACCGCCGATGCCGGCGGTGCGGTCCGGATCGCGGCGATGGAAGGCATCGGCAGCTTCTATC
TCACGCCCTGCATCGTCGACTTCAATAAGATCTATCCGTCGATCCAGATCGAACTGATCACCGATACCCGCCTGCTCGAC
CTCAGCCGTCGCGAGGCCGATATCTTCGTCAGTTTCTTCAGGCCTCACGGCAAGCGATTGTCGATCAAGAAGGTCGGCGA
GTTCCGAATTTCGTTGTACGCGTCCAATCGGTATTTCGAGGGCCGGAGCGAGCCGCGAACGCAGAAGGAACTGGAAGACC
ACGCCTTCATCGACTTCATCGAAGATCACATCTACAGCAAGGAGAATCGCTGGCTCTCCGATGTGTTGCGACCACCGCAT
ACGATCTTTCGCAGCACCAGCCTGGTCGCGCAATCCATCGCCGTGTCCGCAGGGCAGGGAATCGCGATGCTGCCCACCTA
TGTGGCCTCAGGTCGCTCCGAGCTGCGTCCGGTCATGCCGGAACTGTTCACGACCCGCGATATCTGGCTCTCGGTCCATG
AGGACCTGCTCCACATCGCCCGCATCAAAGCCGTCATCACTTTCCTCGAGGAGCGTATCGAGGCCGACAGGACGTTCCTG
AATTCGGTGAAGAACCGACCAGGTCGGAGGCGCCCGTGA

Upstream 100 bases:

>100_bases
CAGAAGCTGATGATCCCGAAGGTCGACATGTTTGCCAACCGGCTGGTGGAGACCTACGCTCCGATCGTCGCGGCCTAGCC
ACTTGACGGGTGACCGGCCG

Downstream 100 bases:

>100_bases
GAGACGCCAAGTGTGGAGCGCGAGCGATCCGGCGTCAGGTCGCGGTGTCGATCAGGCTCTCCAGCAGCCGCTTCAGTTCG
GCGGTGGCCTTGTCGCCGTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTVARSAQQRRSPRRTAEHVAKTQRKIRYDWNDVVYFLEVARQRSLVRAAAKLKVDHTTVSRRIRELERSLNSTLFKRSK
SGFSLTEIGMRLLQYAEGMESQANAIVEAIGTDTADAGGAVRIAAMEGIGSFYLTPCIVDFNKIYPSIQIELITDTRLLD
LSRREADIFVSFFRPHGKRLSIKKVGEFRISLYASNRYFEGRSEPRTQKELEDHAFIDFIEDHIYSKENRWLSDVLRPPH
TIFRSTSLVAQSIAVSAGQGIAMLPTYVASGRSELRPVMPELFTTRDIWLSVHEDLLHIARIKAVITFLEERIEADRTFL
NSVKNRPGRRRP

Sequences:

>Translated_332_residues
MTVARSAQQRRSPRRTAEHVAKTQRKIRYDWNDVVYFLEVARQRSLVRAAAKLKVDHTTVSRRIRELERSLNSTLFKRSK
SGFSLTEIGMRLLQYAEGMESQANAIVEAIGTDTADAGGAVRIAAMEGIGSFYLTPCIVDFNKIYPSIQIELITDTRLLD
LSRREADIFVSFFRPHGKRLSIKKVGEFRISLYASNRYFEGRSEPRTQKELEDHAFIDFIEDHIYSKENRWLSDVLRPPH
TIFRSTSLVAQSIAVSAGQGIAMLPTYVASGRSELRPVMPELFTTRDIWLSVHEDLLHIARIKAVITFLEERIEADRTFL
NSVKNRPGRRRP
>Mature_331_residues
TVARSAQQRRSPRRTAEHVAKTQRKIRYDWNDVVYFLEVARQRSLVRAAAKLKVDHTTVSRRIRELERSLNSTLFKRSKS
GFSLTEIGMRLLQYAEGMESQANAIVEAIGTDTADAGGAVRIAAMEGIGSFYLTPCIVDFNKIYPSIQIELITDTRLLDL
SRREADIFVSFFRPHGKRLSIKKVGEFRISLYASNRYFEGRSEPRTQKELEDHAFIDFIEDHIYSKENRWLSDVLRPPHT
IFRSTSLVAQSIAVSAGQGIAMLPTYVASGRSELRPVMPELFTTRDIWLSVHEDLLHIARIKAVITFLEERIEADRTFLN
SVKNRPGRRRP

Specific function: Transcription activation of the irgA gene. In the presence of sufficient iron, transcription of both irgA and irgB is negatively regulated by a fur-like protein. In low iron conditions, negative regulation of transcription is removed, and production of ir

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=149, Percent_Identity=36.9127516778523, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789440, Length=304, Percent_Identity=23.6842105263158, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787589, Length=205, Percent_Identity=27.3170731707317, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787128, Length=194, Percent_Identity=25.7731958762887, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=24.0677966101695, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=24.6575342465753, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=183, Percent_Identity=25.6830601092896, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=281, Percent_Identity=23.8434163701068, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38072; Mature: 37941

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVARSAQQRRSPRRTAEHVAKTQRKIRYDWNDVVYFLEVARQRSLVRAAAKLKVDHTTV
CCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRIRELERSLNSTLFKRSKSGFSLTEIGMRLLQYAEGMESQANAIVEAIGTDTADAGGA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCE
VRIAAMEGIGSFYLTPCIVDFNKIYPSIQIELITDTRLLDLSRREADIFVSFFRPHGKRL
EEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHEEECCCCCEE
SIKKVGEFRISLYASNRYFEGRSEPRTQKELEDHAFIDFIEDHIYSKENRWLSDVLRPPH
EHEECCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCH
TIFRSTSLVAQSIAVSAGQGIAMLPTYVASGRSELRPVMPELFTTRDIWLSVHEDLLHIA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIKAVITFLEERIEADRTFLNSVKNRPGRRRP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVARSAQQRRSPRRTAEHVAKTQRKIRYDWNDVVYFLEVARQRSLVRAAAKLKVDHTTV
CCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRIRELERSLNSTLFKRSKSGFSLTEIGMRLLQYAEGMESQANAIVEAIGTDTADAGGA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCE
VRIAAMEGIGSFYLTPCIVDFNKIYPSIQIELITDTRLLDLSRREADIFVSFFRPHGKRL
EEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHEEECCCCCEE
SIKKVGEFRISLYASNRYFEGRSEPRTQKELEDHAFIDFIEDHIYSKENRWLSDVLRPPH
EHEECCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCH
TIFRSTSLVAQSIAVSAGQGIAMLPTYVASGRSELRPVMPELFTTRDIWLSVHEDLLHIA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIKAVITFLEERIEADRTFLNSVKNRPGRRRP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1705025 [H]