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The map label for this gene is 86748061

Identifier: 86748061

GI number: 86748061

Start: 1082354

End: 1084027

Strand: Direct

Name: 86748061

Synonym: RPB_0936

Alternate gene names: NA

Gene position: 1082354-1084027 (Clockwise)

Preceding gene: 86748060

Following gene: 86748062

Centisome position: 20.3

GC content: 65.47

Gene sequence:

>1674_bases
ATGACGAAGCGCCGGTTTCGCGCCGCTGCGGTGCAGACACTCGCCAAGTTGGGCGATTTCGACTTCAACATCGCACTCGC
GACTCGCTACGTCGAAGACGCGGTTCGCCAGGGCGCCGAGCTGATCGTATTTCCGGAGTGCATGGACACCGGCTATCTGT
TCGATTCGCCGGAGCATTGCCGCGAACTGGCGGAGACGCTGGCCGATGGTCCGTTCGTCAAGGCGCTGGCGGCGCTCAGC
CGCAAGCACGGCGTCTATATCGCCAGCGGCATCACCGAATGGGATCCCGCCAAAGAGAAGATCTTCAACACCGGCATCAT
GTTCGATCGCAAGGGTGAGGTCGCCTGCCACTATCACAAGCAGTTTCTCGCCACCCACGATCAGAACTGGTTCGCCTTCG
GCGAGCGCGGCTGCCCGGTGGTCGATACCGACCTCGGCAGGATCGGCCTGCTGATCTGCTTCGACGGCCGCATCCCCGAA
ATCTTCCGCGCCATGACGATGCAGGGCGCCGAGGTGATCGTCGACATGGCCAATTTCTTCGCGATGGATCAGGCCGACAT
GTGGGGCCCGGCGCGAAGCTACGAGAACGGCGTCTGGCTGGTGGCCGCCACCAAGGCGGGCTACGAGCGCTCGATCTACT
ATCCGGGCGGCAGCATGATCGTCGATCCGAAGGGCCGGGTGCTGTCGAAGGTGCCGTACGACACCCACGGCATGTCGATC
GCGACGATCGATCTCGACGCCGCCGCCGACAAATCGATCTACACCGCCAACGACAAGATCGCCGATCGCCGTCCGGAGAC
CTACGGCATCATGGCGCTGCCGTACGAGCAGACCCCGGTCTACGGCGTCGCCGATCGTCCGCTGATCCCGTCGAAATCCG
TCACCAAGGTGGCGGCGGTGCAGATTCACGTCACGCCGGAATGCAGCGTCGCGGAGGTGCTCGACATGGTCGACCACACC
GCCAAGCTCGGCGCCAAGGTGCTGGTGCTGCCGGAATACGCATTCTCCGAGCACTACATCCTTTCCGTCGAGGAGGCGTC
CGAGCAGGCCGACCGGACGGCGGAGAATTTGGCGGCAGTCGCGAAGATCGCCGCGCGCTACGGCTGCCTGATCGCGGCGC
CGGTCATCGAGCGCGCCGCCGCCGGGCTCTATGTGACCACCGTGCTGATCGGCCCCGACGGCAAGGAGATCGGCCGCTAC
CGCAAGACACATCTCACCGCCGAAGAGCGCCGGTGGGCGGCCGCTGGCCGTGACTATCCGGTGTTCGAGACGCCGTTCGG
CCGCATCGGCGTGATGTCGGGCTACGATGCGGTGTTTCCGGAGACCTCGCGCTGTCTCGCGATCGGCGGCGCCGATATCA
TTCTGTGGCCCGCCGCGTTGCGCGAGCCGTTCGAGCGCGAGCTCATCGCCGTGCCGCGCGCCGAGGACAACCGGGTGGCG
GTCGTGCTCGCCAACCGCGTCGACAGCCCGTATCCGGGCGGCAGCGTCGTGATCCCGCCGACCGGCTTCCCGCAATGGGA
CATCAACATCGCCGCTCCGCGGGTGATGAAACTCGGCGCGGTCATGCCCAAGCACATAGATCTGGCGGTCTGCCGCCAGA
AGCTGATGATCCCGAAGGTCGACATGTTTGCCAACCGGCTGGTGGAGACCTACGCTCCGATCGTCGCGGCCTAG

Upstream 100 bases:

>100_bases
CCATTGCCTCGATCGCTTCTGGCAGGAAGTCAAGAAATGACGGGCGCCGGCTGAGCCGGGTCCGAGGAATCCGAACCACC
ATCAGAACCTGGAGACTGAC

Downstream 100 bases:

>100_bases
CCACTTGACGGGTGACCGGCCGATGACGGTCGCGCGATCGGCGCAGCAGCGCCGGTCGCCCCGCAGGACGGCAGAGCACG
TGGCCAAGACACAACGCAAG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MTKRRFRAAAVQTLAKLGDFDFNIALATRYVEDAVRQGAELIVFPECMDTGYLFDSPEHCRELAETLADGPFVKALAALS
RKHGVYIASGITEWDPAKEKIFNTGIMFDRKGEVACHYHKQFLATHDQNWFAFGERGCPVVDTDLGRIGLLICFDGRIPE
IFRAMTMQGAEVIVDMANFFAMDQADMWGPARSYENGVWLVAATKAGYERSIYYPGGSMIVDPKGRVLSKVPYDTHGMSI
ATIDLDAAADKSIYTANDKIADRRPETYGIMALPYEQTPVYGVADRPLIPSKSVTKVAAVQIHVTPECSVAEVLDMVDHT
AKLGAKVLVLPEYAFSEHYILSVEEASEQADRTAENLAAVAKIAARYGCLIAAPVIERAAAGLYVTTVLIGPDGKEIGRY
RKTHLTAEERRWAAAGRDYPVFETPFGRIGVMSGYDAVFPETSRCLAIGGADIILWPAALREPFERELIAVPRAEDNRVA
VVLANRVDSPYPGGSVVIPPTGFPQWDINIAAPRVMKLGAVMPKHIDLAVCRQKLMIPKVDMFANRLVETYAPIVAA

Sequences:

>Translated_557_residues
MTKRRFRAAAVQTLAKLGDFDFNIALATRYVEDAVRQGAELIVFPECMDTGYLFDSPEHCRELAETLADGPFVKALAALS
RKHGVYIASGITEWDPAKEKIFNTGIMFDRKGEVACHYHKQFLATHDQNWFAFGERGCPVVDTDLGRIGLLICFDGRIPE
IFRAMTMQGAEVIVDMANFFAMDQADMWGPARSYENGVWLVAATKAGYERSIYYPGGSMIVDPKGRVLSKVPYDTHGMSI
ATIDLDAAADKSIYTANDKIADRRPETYGIMALPYEQTPVYGVADRPLIPSKSVTKVAAVQIHVTPECSVAEVLDMVDHT
AKLGAKVLVLPEYAFSEHYILSVEEASEQADRTAENLAAVAKIAARYGCLIAAPVIERAAAGLYVTTVLIGPDGKEIGRY
RKTHLTAEERRWAAAGRDYPVFETPFGRIGVMSGYDAVFPETSRCLAIGGADIILWPAALREPFERELIAVPRAEDNRVA
VVLANRVDSPYPGGSVVIPPTGFPQWDINIAAPRVMKLGAVMPKHIDLAVCRQKLMIPKVDMFANRLVETYAPIVAA
>Mature_556_residues
TKRRFRAAAVQTLAKLGDFDFNIALATRYVEDAVRQGAELIVFPECMDTGYLFDSPEHCRELAETLADGPFVKALAALSR
KHGVYIASGITEWDPAKEKIFNTGIMFDRKGEVACHYHKQFLATHDQNWFAFGERGCPVVDTDLGRIGLLICFDGRIPEI
FRAMTMQGAEVIVDMANFFAMDQADMWGPARSYENGVWLVAATKAGYERSIYYPGGSMIVDPKGRVLSKVPYDTHGMSIA
TIDLDAAADKSIYTANDKIADRRPETYGIMALPYEQTPVYGVADRPLIPSKSVTKVAAVQIHVTPECSVAEVLDMVDHTA
KLGAKVLVLPEYAFSEHYILSVEEASEQADRTAENLAAVAKIAARYGCLIAAPVIERAAAGLYVTTVLIGPDGKEIGRYR
KTHLTAEERRWAAAGRDYPVFETPFGRIGVMSGYDAVFPETSRCLAIGGADIILWPAALREPFERELIAVPRAEDNRVAV
VLANRVDSPYPGGSVVIPPTGFPQWDINIAAPRVMKLGAVMPKHIDLAVCRQKLMIPKVDMFANRLVETYAPIVAA

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=284, Percent_Identity=27.8169014084507, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI297632350, Length=284, Percent_Identity=27.8169014084507, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI297632348, Length=284, Percent_Identity=27.8169014084507, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI9910460, Length=206, Percent_Identity=30.0970873786408, Blast_Score=83, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556280, Length=261, Percent_Identity=28.3524904214559, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=307, Percent_Identity=28.9902280130293, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=282, Percent_Identity=25.531914893617, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322335, Length=285, Percent_Identity=21.4035087719298, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17933642, Length=301, Percent_Identity=27.906976744186, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355835, Length=259, Percent_Identity=26.2548262548263, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 61203; Mature: 61072

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRRFRAAAVQTLAKLGDFDFNIALATRYVEDAVRQGAELIVFPECMDTGYLFDSPEHC
CCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHH
RELAETLADGPFVKALAALSRKHGVYIASGITEWDPAKEKIFNTGIMFDRKGEVACHYHK
HHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCEEEECCCCEEEHHHH
QFLATHDQNWFAFGERGCPVVDTDLGRIGLLICFDGRIPEIFRAMTMQGAEVIVDMANFF
HHHHHCCCCEEEECCCCCCEEECCCCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHH
AMDQADMWGPARSYENGVWLVAATKAGYERSIYYPGGSMIVDPKGRVLSKVPYDTHGMSI
CCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCEEECCCCCEEEECCCCCCCCEE
ATIDLDAAADKSIYTANDKIADRRPETYGIMALPYEQTPVYGVADRPLIPSKSVTKVAAV
EEEEECCCCCCCEEECCCCHHCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCEEEEE
QIHVTPECSVAEVLDMVDHTAKLGAKVLVLPEYAFSEHYILSVEEASEQADRTAENLAAV
EEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHH
AKIAARYGCLIAAPVIERAAAGLYVTTVLIGPDGKEIGRYRKTHLTAEERRWAAAGRDYP
HHHHHHHCCEEEHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCC
VFETPFGRIGVMSGYDAVFPETSRCLAIGGADIILWPAALREPFERELIAVPRAEDNRVA
CEECCCCCEEECCCCCCCCCCCCCEEEECCCCEEEECHHHCCCHHHCEEECCCCCCCEEE
VVLANRVDSPYPGGSVVIPPTGFPQWDINIAAPRVMKLGAVMPKHIDLAVCRQKLMIPKV
EEEECCCCCCCCCCEEEECCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCH
DMFANRLVETYAPIVAA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKRRFRAAAVQTLAKLGDFDFNIALATRYVEDAVRQGAELIVFPECMDTGYLFDSPEHC
CCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHH
RELAETLADGPFVKALAALSRKHGVYIASGITEWDPAKEKIFNTGIMFDRKGEVACHYHK
HHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCEEEECCCCEEEHHHH
QFLATHDQNWFAFGERGCPVVDTDLGRIGLLICFDGRIPEIFRAMTMQGAEVIVDMANFF
HHHHHCCCCEEEECCCCCCEEECCCCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHH
AMDQADMWGPARSYENGVWLVAATKAGYERSIYYPGGSMIVDPKGRVLSKVPYDTHGMSI
CCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCEEECCCCCEEEECCCCCCCCEE
ATIDLDAAADKSIYTANDKIADRRPETYGIMALPYEQTPVYGVADRPLIPSKSVTKVAAV
EEEEECCCCCCCEEECCCCHHCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCEEEEE
QIHVTPECSVAEVLDMVDHTAKLGAKVLVLPEYAFSEHYILSVEEASEQADRTAENLAAV
EEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHH
AKIAARYGCLIAAPVIERAAAGLYVTTVLIGPDGKEIGRYRKTHLTAEERRWAAAGRDYP
HHHHHHHCCEEEHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCC
VFETPFGRIGVMSGYDAVFPETSRCLAIGGADIILWPAALREPFERELIAVPRAEDNRVA
CEECCCCCEEECCCCCCCCCCCCCEEEECCCCEEEECHHHCCCHHHCEEECCCCCCCEEE
VVLANRVDSPYPGGSVVIPPTGFPQWDINIAAPRVMKLGAVMPKHIDLAVCRQKLMIPKV
EEEECCCCCCCCCCEEEECCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCH
DMFANRLVETYAPIVAA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]