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The map label for this gene is folC [H]

Identifier: 86747758

GI number: 86747758

Start: 717143

End: 718489

Strand: Reverse

Name: folC [H]

Synonym: RPB_0632

Alternate gene names: 86747758

Gene position: 718489-717143 (Counterclockwise)

Preceding gene: 86747759

Following gene: 86747757

Centisome position: 13.48

GC content: 69.41

Gene sequence:

>1347_bases
GTGAGCGCAGCGACAACGCCAAAGCCGTCAGCCTCGGTCGGCGACCTCCGCGCGCGCCTGGCGCGACTGCATCCCGCGCA
GATCGATCTGACGCTCGGCCGAATCGAACGGCTGCTGGCGGCGCTGGATTACCCTGAACGCCGATTGCCGCCGGTGATCC
ATATCGCCGGCACCAACGGCAAGGGCTCCACCCTCGCCTTTCTCCGCGCCATTCTCGAAGCCGCGGGCCTCTGCGTCCAC
GCCTACACTTCGCCGCATCTGGTGCGCGTCAACGAGACCATCCGGCTGGGGCGGCCGGGCGGTGGCGTGCTGGTGAGCGA
CGAAGAGCTGGCCGACGCGCTTTCGCATTGCGAGCGGGTCAATGACGGCGCGCCGATCACGCTGTTCGAGATCGAGACTG
CCGCGGCGTTGTATCTGTTCGCGCAGCATCCCGCCGACGTGGTCCTGCTCGAAGTCGGCCTCGGCGGCCGGCTCGACGCC
ACCAATGTGATCGCGCATCCGCTCGCCTGCGTGCTGACGCCGATCGGGATCGATCACACCGAATTTCTCGGGCCGACGCT
CGCCGAGATCGCCGCCCAAAAGGCCGGCATCATCCGCCGCGACGCGCCGGTGATCGTTGCCGAGCAACCCGACGACGCGA
TGGTGGTGATCGAGCGCGAGGCCAAGCGGCTGCGCGCGCCGCTGCACGCGCGCGGCCAGCACTGGCATGTCGAACTCGAA
CACGGCCGCCTCGCCTATCAGGACGAGCGCGGCCTGATGGACCTCACCGCGCCAAAGCTGTTCGGACGGCATCAGATCGA
CAATGCCGGGCTGGCGATCGCGGCGCTGCGCGCGCAGCAGCGCTTCGCCATCGATCAGGCCGCCTATCAGGCCGGGCTGC
TCGCCGCGGAATGGCCGGCGCGGATGCAGCGGCTGACGTCGGGCACACTGCTCGATGACGCGCCGCAAGGCTGCGAGCTG
TGGCTCGACGGCGGCCATAATGCCGATGGTGGCCGCGTCGCCGCCGCCGCGCTGGGCGATCTCGAAGAGCGGGTATCGCG
GCCGCTGGTGATCATCGCCGGCATGATGGCCAACAAGGACGCCGGCGCGTTCCTCGCCAATTTCACCGGGCTGACGCGCC
ATCTCATCGCCGTGCCGATCCCCGATCGCGACGGCGCGATGCTCCCCGACCGACTCGCGGAGGCAGGGCGTGCGCTCGGC
CTGCGGGTCGAGCTCGCCGACGATGTCGCGGCGGCTCTGCGCCGAATCGCAGGCCTTGCCTACGAACTGCCGCCGCGTAT
CCTGATCACCGGCTCGCTGTATCTCGCCGGCCATGTGCTCAGCCTCAACGGCACGCCGCCGAACTAA

Upstream 100 bases:

>100_bases
CGGCCGTCGTCGACGACGATGCCCAGGATATCGAGGCCGTCGCGACCGAGATCGTGGCGACGCCGCCCGAGCCGGCGCCG
CCGCCGGCCGCGCCGCAGGC

Downstream 100 bases:

>100_bases
CAAAAGCAAAAAGGAGACGACATGCGGATCGCCGCCATCGCCGACATGCACAGCAACTACGCGGCGCTGGAGGCGGTGCT
GGCCGACATCGGCCGGCAAG

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSAATTPKPSASVGDLRARLARLHPAQIDLTLGRIERLLAALDYPERRLPPVIHIAGTNGKGSTLAFLRAILEAAGLCVH
AYTSPHLVRVNETIRLGRPGGGVLVSDEELADALSHCERVNDGAPITLFEIETAAALYLFAQHPADVVLLEVGLGGRLDA
TNVIAHPLACVLTPIGIDHTEFLGPTLAEIAAQKAGIIRRDAPVIVAEQPDDAMVVIEREAKRLRAPLHARGQHWHVELE
HGRLAYQDERGLMDLTAPKLFGRHQIDNAGLAIAALRAQQRFAIDQAAYQAGLLAAEWPARMQRLTSGTLLDDAPQGCEL
WLDGGHNADGGRVAAAALGDLEERVSRPLVIIAGMMANKDAGAFLANFTGLTRHLIAVPIPDRDGAMLPDRLAEAGRALG
LRVELADDVAAALRRIAGLAYELPPRILITGSLYLAGHVLSLNGTPPN

Sequences:

>Translated_448_residues
MSAATTPKPSASVGDLRARLARLHPAQIDLTLGRIERLLAALDYPERRLPPVIHIAGTNGKGSTLAFLRAILEAAGLCVH
AYTSPHLVRVNETIRLGRPGGGVLVSDEELADALSHCERVNDGAPITLFEIETAAALYLFAQHPADVVLLEVGLGGRLDA
TNVIAHPLACVLTPIGIDHTEFLGPTLAEIAAQKAGIIRRDAPVIVAEQPDDAMVVIEREAKRLRAPLHARGQHWHVELE
HGRLAYQDERGLMDLTAPKLFGRHQIDNAGLAIAALRAQQRFAIDQAAYQAGLLAAEWPARMQRLTSGTLLDDAPQGCEL
WLDGGHNADGGRVAAAALGDLEERVSRPLVIIAGMMANKDAGAFLANFTGLTRHLIAVPIPDRDGAMLPDRLAEAGRALG
LRVELADDVAAALRRIAGLAYELPPRILITGSLYLAGHVLSLNGTPPN
>Mature_447_residues
SAATTPKPSASVGDLRARLARLHPAQIDLTLGRIERLLAALDYPERRLPPVIHIAGTNGKGSTLAFLRAILEAAGLCVHA
YTSPHLVRVNETIRLGRPGGGVLVSDEELADALSHCERVNDGAPITLFEIETAAALYLFAQHPADVVLLEVGLGGRLDAT
NVIAHPLACVLTPIGIDHTEFLGPTLAEIAAQKAGIIRRDAPVIVAEQPDDAMVVIEREAKRLRAPLHARGQHWHVELEH
GRLAYQDERGLMDLTAPKLFGRHQIDNAGLAIAALRAQQRFAIDQAAYQAGLLAAEWPARMQRLTSGTLLDDAPQGCELW
LDGGHNADGGRVAAAALGDLEERVSRPLVIIAGMMANKDAGAFLANFTGLTRHLIAVPIPDRDGAMLPDRLAEAGRALGL
RVELADDVAAALRRIAGLAYELPPRILITGSLYLAGHVLSLNGTPPN

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=349, Percent_Identity=30.6590257879656, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI66932990, Length=349, Percent_Identity=30.6590257879656, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1788654, Length=437, Percent_Identity=30.4347826086957, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17553150, Length=491, Percent_Identity=25.8655804480652, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17553148, Length=491, Percent_Identity=25.8655804480652, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71984923, Length=491, Percent_Identity=25.8655804480652, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323760, Length=437, Percent_Identity=29.2906178489703, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6324815, Length=436, Percent_Identity=26.3761467889908, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24641571, Length=214, Percent_Identity=32.2429906542056, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24581568, Length=204, Percent_Identity=30.3921568627451, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47777; Mature: 47645

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAATTPKPSASVGDLRARLARLHPAQIDLTLGRIERLLAALDYPERRLPPVIHIAGTNG
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCHHHCCCEEEEECCCC
KGSTLAFLRAILEAAGLCVHAYTSPHLVRVNETIRLGRPGGGVLVSDEELADALSHCERV
CCHHHHHHHHHHHHCCEEEEECCCCEEEEECCEEEECCCCCCEEECHHHHHHHHHHHHHC
NDGAPITLFEIETAAALYLFAQHPADVVLLEVGLGGRLDATNVIAHPLACVLTPIGIDHT
CCCCCEEEEEECCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHH
EFLGPTLAEIAAQKAGIIRRDAPVIVAEQPDDAMVVIEREAKRLRAPLHARGQHWHVELE
HHCCHHHHHHHHHHCCCEECCCCEEEEECCCCCEEEEECHHHHHCCCCCCCCCEEEEEEE
HGRLAYQDERGLMDLTAPKLFGRHQIDNAGLAIAALRAQQRFAIDQAAYQAGLLAAEWPA
CCEEEEECCCCCEEECCHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCEEHHHHH
RMQRLTSGTLLDDAPQGCELWLDGGHNADGGRVAAAALGDLEERVSRPLVIIAGMMANKD
HHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHCCCEEEEEEECCCCC
AGAFLANFTGLTRHLIAVPIPDRDGAMLPDRLAEAGRALGLRVELADDVAAALRRIAGLA
CCHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHCCHHEEEEEEEHHHHHHHHHHHHCCC
YELPPRILITGSLYLAGHVLSLNGTPPN
CCCCCCEEEEEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
SAATTPKPSASVGDLRARLARLHPAQIDLTLGRIERLLAALDYPERRLPPVIHIAGTNG
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCHHHCCCEEEEECCCC
KGSTLAFLRAILEAAGLCVHAYTSPHLVRVNETIRLGRPGGGVLVSDEELADALSHCERV
CCHHHHHHHHHHHHCCEEEEECCCCEEEEECCEEEECCCCCCEEECHHHHHHHHHHHHHC
NDGAPITLFEIETAAALYLFAQHPADVVLLEVGLGGRLDATNVIAHPLACVLTPIGIDHT
CCCCCEEEEEECCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHH
EFLGPTLAEIAAQKAGIIRRDAPVIVAEQPDDAMVVIEREAKRLRAPLHARGQHWHVELE
HHCCHHHHHHHHHHCCCEECCCCEEEEECCCCCEEEEECHHHHHCCCCCCCCCEEEEEEE
HGRLAYQDERGLMDLTAPKLFGRHQIDNAGLAIAALRAQQRFAIDQAAYQAGLLAAEWPA
CCEEEEECCCCCEEECCHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCEEHHHHH
RMQRLTSGTLLDDAPQGCELWLDGGHNADGGRVAAAALGDLEERVSRPLVIIAGMMANKD
HHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHCCCEEEEEEECCCCC
AGAFLANFTGLTRHLIAVPIPDRDGAMLPDRLAEAGRALGLRVELADDVAAALRRIAGLA
CCHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHCCHHEEEEEEEHHHHHHHHHHHHCCC
YELPPRILITGSLYLAGHVLSLNGTPPN
CCCCCCEEEEEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]