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The map label for this gene is pnp [H]

Identifier: 86747731

GI number: 86747731

Start: 678925

End: 681081

Strand: Direct

Name: pnp [H]

Synonym: RPB_0605

Alternate gene names: 86747731

Gene position: 678925-681081 (Clockwise)

Preceding gene: 86747730

Following gene: 86747732

Centisome position: 12.73

GC content: 65.46

Gene sequence:

>2157_bases
ATGTTCAATAAGCATTCCGTCGAAATCGATTGGGGTGGGCGTCCCCTCAAGCTCGAGACCGGCAAGGTCGCGCGCCAGGC
CGACGGCGCCGTCGTCGCCACCTATGGCGAAACCATCGTGCTCGCCACCGTGGTGGCCGCCAAGACGCCGCGCGAAGGCG
TCGATTTCCTGCCGCTGACCGTGGACTATCAGGAAAAGACCTACGCGGCCGGCCGCATCCCCGGCGGTTACTTCAAGCGC
GAGGGTCGCCCGACCGAGAAGGAGACGCTGGTCTCCCGCCTGATCGACCGCCCGATCCGTCCGTTGTTCGCCGACGGCTG
GCGCAACGAGACCCAGGTGATCGTCACCGTGCTGTCGCACGACATGGAGAACGATCCGGACATTCTGGCGATGGTCGCCG
CCTCGGCGGCGCTGACGCTGTCCGGCGCGCCGTTCAAGGGCCCGATCGGCGCCGCCCGCGTCGGCTTCATCAACGACGAA
TACGTGCTCAACCCGGTGCTCGACGAGATGCCCGAGACCCAGCTCGAGCTGGTCGTCGCCGGCACCGCCGACGCGGTGCT
GATGGTCGAATCCGAAGCCAAGGAGCTGTCGGAAGAAATCATGCTCGGCGCGGTGATGTTCGGTCACCGTCACTTCCAGC
CGGTGATCGACGCGATCATCGAGCTCGCCGAGAAGGCCGCCAAGGAGCCGCGCGAACTCGTCGTCATCGACGATTCGGCG
ATCGAGAAGGAAATGCTCGGCCTGGTCGAGCAGGAACTGCGCGCCGCCTACGCGATCCCGGTCAAGCAGGAGCGCTACGC
CGCGGTCGGCAAGGTCAAGGAAAAGGCGATCGCGCACTTCTTCCCGGAAGGCGAAGAGCCGAAATACGACAAGCTGCGCA
TCGCCGGCGTGTTCAAGGAGCTCGAGGCCAAGATCGTTCGCTGGAACATCCTCGACACCGGCAAGCGCATCGACGGCCGT
GACAGCAAGACCGTCCGCAGCATCATCGCCGAAGCCGGCGTGCTGCCGCGCGCCCACGGTTCGGCGCTGTTCACCCGCGG
CGAGACCCAGGCGCTGGTGGTCACCACGCTCGGCACCGGCGAAGACGAACAGTACGTCGACTCGCTGGCCGGAACGTACA
AAGAGACGTTCCTGCTGCACTACAACTTCCCTCCCTATTCGGTCGGCGAGACCGGCCGCCTCGGTGGTACCAAGCGCCGA
GAGATCGGCCACGGCAAGCTGGCGTGGCGCGCGATCCGTCCGGTGCTGCCGCCGCATCACGAGTTCCCCTACACCATCCG
CGTCGTCTCCGAGATCACCGAGTCGAACGGCTCGTCCTCGATGGCGTCGGTGTGCGGCGCCTCGCTGGCGCTGATGGATG
CCGGCGTGCCGCTGAAGCGGCCGACCGCGGGCATCGCGATGGGGCTGATCCTGGAAGGCGAGCGCTTCGCGGTGCTCAGC
GATATTCTGGGCGACGAGGATCATCTCGGCGACATGGACTTCAAGGTGGCCGGCACCGAGCAGGGCATCACCTCGCTGCA
GATGGACATCAAGATCGCCGGCATCACCGAAGAGATCATGAAGGTGGCGCTCGGCCAGGCCAAGGACGGCCGCATCCACA
TCCTGGGTGAGATGTCCAAGGCGCTCGACCGCGCCCGCGCCGAGCTCGGCGAACACGCGCCGCGCATCGAGACCTTCAAG
ATCCCGACCGACAAGATCCGTGAAGTGATCGGCACCGGCGGCAAGGTGATCCGCGAGATCGTCGAGAAGACCGGCGCCAA
GGTCAACATCGAGGACGACGGCACCGTCAAGGTCGCCTCCAGCGATGGTGAGTCGATCAAGGCTGCTATCAAGTGGATCA
AGTCGATCGCCTCCGATCCGGAAGTCGGCGAGATCTACGAAGGCACCGTCGTCAAGGTGATGGAGTTCGGCGCCTTCGTG
AACTTCTTCGGCGCCAAGGACGGCCTGGTGCATATCAGCCAGCTCGCCTCCGGCCGCGTGCAGAAGACCTCCGACGTCGT
CAAGGAAGGCGACAAGGTCAAGGTCAAGCTGCTCGGCTTCGACGATCGCGGCAAGACCCGGCTGTCGATGCGCGTGGTCG
ATCAGGAGACCGGCGAAGACCTCGAGGCCAAGCAGAAGGCCGAAGGCGAAGCCCCGGCCCAGGCCACCGGCGAGTAG

Upstream 100 bases:

>100_bases
GCTGCTCGCACCTTCGGGTCGCCAGCGTCCCGCAATCTTGCGCATGGTCTTTTGGTATCTGGCGTCCGGTCGTCGCGGAA
AACGATGAAAGAGACACCCG

Downstream 100 bases:

>100_bases
TTCGCGGGACCGGAACCGGTCGTGACCCGATCACGGCCGGACCGGCGCGAGATTGCCGCGCAACAGCAAGAATAGGTGAT
CAGGGGCGGCCGCGCAGGCT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MFNKHSVEIDWGGRPLKLETGKVARQADGAVVATYGETIVLATVVAAKTPREGVDFLPLTVDYQEKTYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSHDMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDE
YVLNPVLDEMPETQLELVVAGTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIELAEKAAKEPRELVVIDDSA
IEKEMLGLVEQELRAAYAIPVKQERYAAVGKVKEKAIAHFFPEGEEPKYDKLRIAGVFKELEAKIVRWNILDTGKRIDGR
DSKTVRSIIAEAGVLPRAHGSALFTRGETQALVVTTLGTGEDEQYVDSLAGTYKETFLLHYNFPPYSVGETGRLGGTKRR
EIGHGKLAWRAIRPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFK
IPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFV
NFFGAKDGLVHISQLASGRVQKTSDVVKEGDKVKVKLLGFDDRGKTRLSMRVVDQETGEDLEAKQKAEGEAPAQATGE

Sequences:

>Translated_718_residues
MFNKHSVEIDWGGRPLKLETGKVARQADGAVVATYGETIVLATVVAAKTPREGVDFLPLTVDYQEKTYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSHDMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDE
YVLNPVLDEMPETQLELVVAGTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIELAEKAAKEPRELVVIDDSA
IEKEMLGLVEQELRAAYAIPVKQERYAAVGKVKEKAIAHFFPEGEEPKYDKLRIAGVFKELEAKIVRWNILDTGKRIDGR
DSKTVRSIIAEAGVLPRAHGSALFTRGETQALVVTTLGTGEDEQYVDSLAGTYKETFLLHYNFPPYSVGETGRLGGTKRR
EIGHGKLAWRAIRPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFK
IPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFV
NFFGAKDGLVHISQLASGRVQKTSDVVKEGDKVKVKLLGFDDRGKTRLSMRVVDQETGEDLEAKQKAEGEAPAQATGE
>Mature_718_residues
MFNKHSVEIDWGGRPLKLETGKVARQADGAVVATYGETIVLATVVAAKTPREGVDFLPLTVDYQEKTYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSHDMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDE
YVLNPVLDEMPETQLELVVAGTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIELAEKAAKEPRELVVIDDSA
IEKEMLGLVEQELRAAYAIPVKQERYAAVGKVKEKAIAHFFPEGEEPKYDKLRIAGVFKELEAKIVRWNILDTGKRIDGR
DSKTVRSIIAEAGVLPRAHGSALFTRGETQALVVTTLGTGEDEQYVDSLAGTYKETFLLHYNFPPYSVGETGRLGGTKRR
EIGHGKLAWRAIRPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFK
IPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFV
NFFGAKDGLVHISQLASGRVQKTSDVVKEGDKVKVKLLGFDDRGKTRLSMRVVDQETGEDLEAKQKAEGEAPAQATGE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=717, Percent_Identity=37.9358437935844, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=50, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI21361576, Length=87, Percent_Identity=47.1264367816092, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI145693187, Length=696, Percent_Identity=57.0402298850575, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=83, Percent_Identity=46.9879518072289, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=712, Percent_Identity=34.2696629213483, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535281, Length=88, Percent_Identity=46.5909090909091, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320850, Length=98, Percent_Identity=36.734693877551, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=38.1615598885794, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=38.1615598885794, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=38.1615598885794, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=656, Percent_Identity=37.8048780487805, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=45.0549450549451, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78030; Mature: 78030

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKHSVEIDWGGRPLKLETGKVARQADGAVVATYGETIVLATVVAAKTPREGVDFLPLT
CCCCEEEEEECCCEEEEEECCCHHHHCCCCEEEECCCEEEEHHHHHHCCCCCCCCEEEEE
VDYQEKTYAAGRIPGGYFKREGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSH
EECCCCCEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHEEEEEECC
DMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDEYVLNPVLDEMPETQLELVVA
CCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCEECHHHHHCCCCEEEEEEE
GTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIELAEKAAKEPRELVVIDDSA
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
IEKEMLGLVEQELRAAYAIPVKQERYAAVGKVKEKAIAHFFPEGEEPKYDKLRIAGVFKE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEHHHHHHHH
LEAKIVRWNILDTGKRIDGRDSKTVRSIIAEAGVLPRAHGSALFTRGETQALVVTTLGTG
HCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYVDSLAGTYKETFLLHYNFPPYSVGETGRLGGTKRREIGHGKLAWRAIRPVLPPHH
CCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEHHHHH
ALDRARAELGEHAPRIETFKIPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVAS
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFVNFFGAKDGLVHISQLASGRV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCC
QKTSDVVKEGDKVKVKLLGFDDRGKTRLSMRVVDQETGEDLEAKQKAEGEAPAQATGE
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MFNKHSVEIDWGGRPLKLETGKVARQADGAVVATYGETIVLATVVAAKTPREGVDFLPLT
CCCCEEEEEECCCEEEEEECCCHHHHCCCCEEEECCCEEEEHHHHHHCCCCCCCCEEEEE
VDYQEKTYAAGRIPGGYFKREGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSH
EECCCCCEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHEEEEEECC
DMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDEYVLNPVLDEMPETQLELVVA
CCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCEECHHHHHCCCCEEEEEEE
GTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIELAEKAAKEPRELVVIDDSA
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
IEKEMLGLVEQELRAAYAIPVKQERYAAVGKVKEKAIAHFFPEGEEPKYDKLRIAGVFKE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEHHHHHHHH
LEAKIVRWNILDTGKRIDGRDSKTVRSIIAEAGVLPRAHGSALFTRGETQALVVTTLGTG
HCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYVDSLAGTYKETFLLHYNFPPYSVGETGRLGGTKRREIGHGKLAWRAIRPVLPPHH
CCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEHHHHH
ALDRARAELGEHAPRIETFKIPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVAS
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFVNFFGAKDGLVHISQLASGRV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCC
QKTSDVVKEGDKVKVKLLGFDDRGKTRLSMRVVDQETGEDLEAKQKAEGEAPAQATGE
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA