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The map label for this gene is truB [H]

Identifier: 86747729

GI number: 86747729

Start: 677254

End: 678309

Strand: Direct

Name: truB [H]

Synonym: RPB_0603

Alternate gene names: 86747729

Gene position: 677254-678309 (Clockwise)

Preceding gene: 86747728

Following gene: 86747730

Centisome position: 12.7

GC content: 67.42

Gene sequence:

>1056_bases
GTGCAGCCCGACGACGCCTCGTCCGCGCAGCTCCGCCAGCCGCGCGACAACAACGATCCGCGCAACAATGCCCGCGGCAA
GGACGGCGGCAACAAGCAGCCGCGCCGCGACAAGCGCGACGTCCACGGCTGGGTGGTGCTCGACAAGCCGATTGGCATGA
CCTCGACCCATGCGGTCGCCGTGGTGAAGCGGCTGTTCTCGGCCAAGCGCGCCGGCCATGCCGGCACGCTCGATCCGCTG
GCCTCGGGCGGGCTGCCGATCGCGATGGGCGAAGCCACCAAGACGGTGCCGTTCGTGATGGACGGCCGCAAGCGCTACCG
CTTCACCGTGGCGTGGGGCGAGGAGCGCGACACCGACGACACCGAAGGCAAGGTCACCCAGACCAGCGACATCCGCCCCA
CCGCCGAGGCGATCCGGGCGCTGCTGCCGCGCTTCACCGGCCTGATCGAGCAGACCCCGCCGCAATATTCCGCCATCAAG
ATCGCCGGCGAGCGCGCCTACGACATGGCGCGCGACGGCGAAATCGTCCCGCTGGTTCCCCGGCCCGTCGAGATCCACGA
ATTAACCCTTGTCGAACATGATGATAGCGGAAAATCCGTGTTCGAGGCCGAATGCGGCAAGGGAACCTATGTGCGGGCGC
TGGCGCGCGACATCGGCCGGCTGCTCGGCTGCTACGGTCATATCTGCGCGCTGCGCCGGACCCTGGTCGGGCCGTTCGAC
GAGGCGGACATGATTCCGCTGGAACAGTTGCAGGCTTTGTGCGATAGAGCCGCGTCGGGCGAGGGCAGCCTCGCCGACGC
GCTTTTGCCCGTTGAGACCGCGCTGGACGACATCCCGGCACTGGCCGTCACACGGGCTGATGCGGCAAGGCTCCATCGGG
GCCAGGCCGTTTTGTTGCGCGGACGGGATGCGCCCAATACTAGCGGCACAGTCTATGTGACGGTGGCAGGCCGGCTTCTG
GCCCTCGCCGAGCTCGGCAATGGCGAACTCATCCCCAAGCGTGTGTTCAACCTCTCCGGACTGACCGCCAGTTCGGCCCG
CAAGGAAAGTATTTGA

Upstream 100 bases:

>100_bases
AGCTGCTGCGTACGCCCGCGGTGCAAAAAGACCTCGAACCCGATTCGGACCAGGACTGATGACGATGACCACCCCCGCCG
CGACGCTCGCTCCGCACGAT

Downstream 100 bases:

>100_bases
CGATGTCGATTACCGCAGAACGCAAAGCGGAAGTCATCCAAGGCAATGCCAACAAGGCCGGCGATACCGGCTCGCCCGAG
GTTCAGGTCGCGATCCTGTC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MQPDDASSAQLRQPRDNNDPRNNARGKDGGNKQPRRDKRDVHGWVVLDKPIGMTSTHAVAVVKRLFSAKRAGHAGTLDPL
ASGGLPIAMGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGKVTQTSDIRPTAEAIRALLPRFTGLIEQTPPQYSAIK
IAGERAYDMARDGEIVPLVPRPVEIHELTLVEHDDSGKSVFEAECGKGTYVRALARDIGRLLGCYGHICALRRTLVGPFD
EADMIPLEQLQALCDRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPNTSGTVYVTVAGRLL
ALAELGNGELIPKRVFNLSGLTASSARKESI

Sequences:

>Translated_351_residues
MQPDDASSAQLRQPRDNNDPRNNARGKDGGNKQPRRDKRDVHGWVVLDKPIGMTSTHAVAVVKRLFSAKRAGHAGTLDPL
ASGGLPIAMGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGKVTQTSDIRPTAEAIRALLPRFTGLIEQTPPQYSAIK
IAGERAYDMARDGEIVPLVPRPVEIHELTLVEHDDSGKSVFEAECGKGTYVRALARDIGRLLGCYGHICALRRTLVGPFD
EADMIPLEQLQALCDRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPNTSGTVYVTVAGRLL
ALAELGNGELIPKRVFNLSGLTASSARKESI
>Mature_351_residues
MQPDDASSAQLRQPRDNNDPRNNARGKDGGNKQPRRDKRDVHGWVVLDKPIGMTSTHAVAVVKRLFSAKRAGHAGTLDPL
ASGGLPIAMGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGKVTQTSDIRPTAEAIRALLPRFTGLIEQTPPQYSAIK
IAGERAYDMARDGEIVPLVPRPVEIHELTLVEHDDSGKSVFEAECGKGTYVRALARDIGRLLGCYGHICALRRTLVGPFD
EADMIPLEQLQALCDRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPNTSGTVYVTVAGRLL
ALAELGNGELIPKRVFNLSGLTASSARKESI

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=215, Percent_Identity=35.8139534883721, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=37.741935483871, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17553978, Length=274, Percent_Identity=25.9124087591241, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=252, Percent_Identity=30.1587301587302, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=131, Percent_Identity=35.1145038167939, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364189, Length=202, Percent_Identity=27.2277227722772, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364187, Length=202, Percent_Identity=27.2277227722772, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364185, Length=202, Percent_Identity=27.2277227722772, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364183, Length=202, Percent_Identity=27.2277227722772, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI62471759, Length=202, Percent_Identity=27.2277227722772, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17975520, Length=202, Percent_Identity=27.2277227722772, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 37897; Mature: 37897

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPDDASSAQLRQPRDNNDPRNNARGKDGGNKQPRRDKRDVHGWVVLDKPIGMTSTHAVA
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
VVKRLFSAKRAGHAGTLDPLASGGLPIAMGEATKTVPFVMDGRKRYRFTVAWGEERDTDD
HHHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCCCCEEECCCEEEEEEEEECCCCCCCC
TEGKVTQTSDIRPTAEAIRALLPRFTGLIEQTPPQYSAIKIAGERAYDMARDGEIVPLVP
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHCCCCCEEEECC
RPVEIHELTLVEHDDSGKSVFEAECGKGTYVRALARDIGRLLGCYGHICALRRTLVGPFD
CCCCEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EADMIPLEQLQALCDRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLR
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEHHHHHHHCCCCEEEEE
GRDAPNTSGTVYVTVAGRLLALAELGNGELIPKRVFNLSGLTASSARKESI
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure
MQPDDASSAQLRQPRDNNDPRNNARGKDGGNKQPRRDKRDVHGWVVLDKPIGMTSTHAVA
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
VVKRLFSAKRAGHAGTLDPLASGGLPIAMGEATKTVPFVMDGRKRYRFTVAWGEERDTDD
HHHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCCCCEEECCCEEEEEEEEECCCCCCCC
TEGKVTQTSDIRPTAEAIRALLPRFTGLIEQTPPQYSAIKIAGERAYDMARDGEIVPLVP
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHCCCCCEEEECC
RPVEIHELTLVEHDDSGKSVFEAECGKGTYVRALARDIGRLLGCYGHICALRRTLVGPFD
CCCCEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EADMIPLEQLQALCDRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLR
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEHHHHHHHCCCCEEEEE
GRDAPNTSGTVYVTVAGRLLALAELGNGELIPKRVFNLSGLTASSARKESI
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA