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The map label for this gene is fgs [H]

Identifier: 86747690

GI number: 86747690

Start: 634509

End: 635825

Strand: Reverse

Name: fgs [H]

Synonym: RPB_0564

Alternate gene names: 86747690

Gene position: 635825-634509 (Counterclockwise)

Preceding gene: 86747691

Following gene: 86747689

Centisome position: 11.93

GC content: 70.39

Gene sequence:

>1317_bases
ATGTTCGACCTGCCGAAATACGGCGACGGAATCTGCCTGGCGCGGCTCGCCGCGGTGCTCGATCGGCTCGGCATCGATCG
CGCAAGGCTGTCGCGCATCGCGATCGCGGTCTGCGGCTCCAACGGCAAGGGCTCGACCGCGGCGTTCGCGGCGAGCATCG
GCAAGGCGTATGGGCTGCGCACCGGGCTGTTCACCTCGCCGCATCTCCTTCGCTTCAACGAGCGCTTCCGGATCGACGGC
GTGCCGATCCCGCACGAGGCGCTGACGCCGCTGCTGACACGCGTGACGGCGGTAGTCGCCGATGTTTCAGCGCAGCGCGG
CGAACAATTCGGCGCGTTCGAGGCGCAGTTCGCGCTGGCGTGTTTGTACTTCCAGCAGGAGAATTGCGACCTCGTGGTGT
GCGAGGCCGGAATCGGCGGTCGCTACGATCCGGTGCGACTGCTCGCCGCGGGCGTGAGCTGCGTCACCTCGGTCGATCTC
GAACACACCGCGCTGCTGGGCCATTCGCTGGAGCTGATCGCCGCCGACAAGAGCGACGCATGCGCCGCGGGCGGCACCAT
CGTCTATGGCGAGAACTGCCGGCCGTTGCGGGATCAGCTCGCCGAATACAATCGCAGCCGGCGTGTCGATGCGCTCTTTG
TCCGCGACGAGATCGGCATCGAAGCCGAGACCATCGTGGATGCGCGGCAGCGCTTCGATCTGCGGATCGGCCGGGCCGGC
TACGCCGCGCTGGAAACGTCGCTGCTCGGCGCGTTCCAGCTCAACAACGCCGCGATCGCCGCGACGCTGTTCGCGCTGTG
GCTGGAGCGGAGCGCGGGCGCCCAAAATCCCGCGCGCGTCGAGGCCGCGATCCGCGACGGCCTGCGCGAGACGCGCTGGC
CGGGGCGGCTGGAGACGATCAATACGGCGCCGCTGACGGTGATCGATGTCGGCCACACCCCGGACGGCATCCGCCAGGCG
CTGGCGGGGCTTCACGCCGCGCATGGCGACAGCGGCTGGATCCTGGTGCTCGGCGTGTCGCGCGACAAGGCGGCCGGCGA
GATCGTCGACGCGCTGGCGCCGGCGTTCGACGCCATCGTCTGCAGCGCCGCGCATCACAAGGGCGCCCCGGCCGAACTGA
TCGCGGAGGCCGCACGCGCGGCCAATCCGCACGCCGAGATTCACACCGCGCCGGCGGTCGCCGATGCGGTGCGCGACGCG
CGTGCGCTGGCCGCGCGGCGCGACGCGCGGATCTATGTCGCCGGCGGGCTGTTCCTCGCGATCGAATTCGCGACGGTGCT
GCGCGGCGGCCGCGCCGAGGATCTGCGGTTCTTCTGA

Upstream 100 bases:

>100_bases
GAATTCCAACCCGGCGATCCGCTTTCTCGAAACATGCGGCCGTGACGATCTGATCCTGTCGATCTGGCGGCACAGCGTCA
CGGCGACGGGATTGTTGTAG

Downstream 100 bases:

>100_bases
CGCATGATTTGAACATTGCGCATCGCGGCGGCGCCGATTAGCGTCCGCCGCCGACGCGACAACAACACTGCACTCAGGGA
GAGCCACATGACCGCCGTCA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MFDLPKYGDGICLARLAAVLDRLGIDRARLSRIAIAVCGSNGKGSTAAFAASIGKAYGLRTGLFTSPHLLRFNERFRIDG
VPIPHEALTPLLTRVTAVVADVSAQRGEQFGAFEAQFALACLYFQQENCDLVVCEAGIGGRYDPVRLLAAGVSCVTSVDL
EHTALLGHSLELIAADKSDACAAGGTIVYGENCRPLRDQLAEYNRSRRVDALFVRDEIGIEAETIVDARQRFDLRIGRAG
YAALETSLLGAFQLNNAAIAATLFALWLERSAGAQNPARVEAAIRDGLRETRWPGRLETINTAPLTVIDVGHTPDGIRQA
LAGLHAAHGDSGWILVLGVSRDKAAGEIVDALAPAFDAIVCSAAHHKGAPAELIAEAARAANPHAEIHTAPAVADAVRDA
RALAARRDARIYVAGGLFLAIEFATVLRGGRAEDLRFF

Sequences:

>Translated_438_residues
MFDLPKYGDGICLARLAAVLDRLGIDRARLSRIAIAVCGSNGKGSTAAFAASIGKAYGLRTGLFTSPHLLRFNERFRIDG
VPIPHEALTPLLTRVTAVVADVSAQRGEQFGAFEAQFALACLYFQQENCDLVVCEAGIGGRYDPVRLLAAGVSCVTSVDL
EHTALLGHSLELIAADKSDACAAGGTIVYGENCRPLRDQLAEYNRSRRVDALFVRDEIGIEAETIVDARQRFDLRIGRAG
YAALETSLLGAFQLNNAAIAATLFALWLERSAGAQNPARVEAAIRDGLRETRWPGRLETINTAPLTVIDVGHTPDGIRQA
LAGLHAAHGDSGWILVLGVSRDKAAGEIVDALAPAFDAIVCSAAHHKGAPAELIAEAARAANPHAEIHTAPAVADAVRDA
RALAARRDARIYVAGGLFLAIEFATVLRGGRAEDLRFF
>Mature_438_residues
MFDLPKYGDGICLARLAAVLDRLGIDRARLSRIAIAVCGSNGKGSTAAFAASIGKAYGLRTGLFTSPHLLRFNERFRIDG
VPIPHEALTPLLTRVTAVVADVSAQRGEQFGAFEAQFALACLYFQQENCDLVVCEAGIGGRYDPVRLLAAGVSCVTSVDL
EHTALLGHSLELIAADKSDACAAGGTIVYGENCRPLRDQLAEYNRSRRVDALFVRDEIGIEAETIVDARQRFDLRIGRAG
YAALETSLLGAFQLNNAAIAATLFALWLERSAGAQNPARVEAAIRDGLRETRWPGRLETINTAPLTVIDVGHTPDGIRQA
LAGLHAAHGDSGWILVLGVSRDKAAGEIVDALAPAFDAIVCSAAHHKGAPAELIAEAARAANPHAEIHTAPAVADAVRDA
RALAARRDARIYVAGGLFLAIEFATVLRGGRAEDLRFF

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=389, Percent_Identity=30.8483290488432, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI66932984, Length=389, Percent_Identity=30.8483290488432, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI1788654, Length=369, Percent_Identity=30.8943089430894, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=369, Percent_Identity=25.2032520325203, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17553150, Length=369, Percent_Identity=25.2032520325203, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17553148, Length=369, Percent_Identity=25.2032520325203, Blast_Score=98, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324815, Length=320, Percent_Identity=27.5, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323760, Length=376, Percent_Identity=24.2021276595745, Blast_Score=90, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24641571, Length=407, Percent_Identity=27.027027027027, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24581568, Length=170, Percent_Identity=30, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46577; Mature: 46577

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDLPKYGDGICLARLAAVLDRLGIDRARLSRIAIAVCGSNGKGSTAAFAASIGKAYGLR
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCC
TGLFTSPHLLRFNERFRIDGVPIPHEALTPLLTRVTAVVADVSAQRGEQFGAFEAQFALA
CCCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
CLYFQQENCDLVVCEAGIGGRYDPVRLLAAGVSCVTSVDLEHTALLGHSLELIAADKSDA
EEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECCCCCC
CAAGGTIVYGENCRPLRDQLAEYNRSRRVDALFVRDEIGIEAETIVDARQRFDLRIGRAG
CCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCEEECCCH
YAALETSLLGAFQLNNAAIAATLFALWLERSAGAQNPARVEAAIRDGLRETRWPGRLETI
HHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
NTAPLTVIDVGHTPDGIRQALAGLHAAHGDSGWILVLGVSRDKAAGEIVDALAPAFDAIV
CCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
CSAAHHKGAPAELIAEAARAANPHAEIHTAPAVADAVRDARALAARRDARIYVAGGLFLA
HHHHCCCCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEECHHHHH
IEFATVLRGGRAEDLRFF
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFDLPKYGDGICLARLAAVLDRLGIDRARLSRIAIAVCGSNGKGSTAAFAASIGKAYGLR
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCC
TGLFTSPHLLRFNERFRIDGVPIPHEALTPLLTRVTAVVADVSAQRGEQFGAFEAQFALA
CCCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
CLYFQQENCDLVVCEAGIGGRYDPVRLLAAGVSCVTSVDLEHTALLGHSLELIAADKSDA
EEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECCCCCC
CAAGGTIVYGENCRPLRDQLAEYNRSRRVDALFVRDEIGIEAETIVDARQRFDLRIGRAG
CCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCEEECCCH
YAALETSLLGAFQLNNAAIAATLFALWLERSAGAQNPARVEAAIRDGLRETRWPGRLETI
HHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
NTAPLTVIDVGHTPDGIRQALAGLHAAHGDSGWILVLGVSRDKAAGEIVDALAPAFDAIV
CCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
CSAAHHKGAPAELIAEAARAANPHAEIHTAPAVADAVRDARALAARRDARIYVAGGLFLA
HHHHCCCCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEECHHHHH
IEFATVLRGGRAEDLRFF
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105929; 9618466 [H]