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The map label for this gene is ycaN [H]

Identifier: 86747653

GI number: 86747653

Start: 588299

End: 589192

Strand: Direct

Name: ycaN [H]

Synonym: RPB_0527

Alternate gene names: 86747653

Gene position: 588299-589192 (Clockwise)

Preceding gene: 86747646

Following gene: 86747654

Centisome position: 11.03

GC content: 67.23

Gene sequence:

>894_bases
ATGAATGCCAAGCCGGATCTCTCCGACCTCACAGCTTTCGCTGCGATCGCGGCTCATCGCAGTTTTCGCAAGGCTGCCAA
TGACTTGGGGGTGTCCGCCTCGACCCTGAGCCACATCATGCGAACCCTGGAGGCCAATCTCGGAGTGAGACTGCTGCACC
GGACCACCCGCAGCGTTGCGCCGACCGAGATCGGCGAACGGCTGCTCGGCCGGCTCAATCCGGTGCTGCGCGATTTCGAC
GCCGCCTTGCAGGACCTCGAGGCGTGGCGCAGCCGGCCGAGCGGCACGTTGCGCATCAATGCCGCCGAGGGCGCGGTACG
GTTGTTGCTGCAAACCGTGGTGCCGGCGTTTTTTGCGCGCCACCCCGAGATGGCGCTCGATCTCGTCACCGAGGCGCGGC
TGGTCGATATCGTCGCGGAAGGCTTCGACGCAGGCATCCGGATCGGCGAGGCGGTGCCGCGCGATATGGTCGCGGTGCGG
TTCGGCGGCGCGATCCGGTTCGTCGCGGTCGCGTCGCCCGGCTATCTCGCCGCGCACCCGCCGCCGCGGACCCCGGATGA
CCTCGTCCATCACGCCTGCATCCGGTTCCGGTTGAAGAGCGGGCAGTTGTTCCGCTGGGAATTCGCGCGTCACGGCCAGG
AATTGGCGGTCGATGTCGCCGGTCCGTTGACGCTCGACCATGTCGAACTGATGGTCGAGGCCGCTGCCGACGGCCTCGGC
ATCGCCTATGTGCCGGACCGGACCGCGCAGCCTTATCTCTCGCGCGGCGCCGTGGTCGGCGTGCTCGATGAGTGGTGTCC
GAAAATCCCGGGGCTGTTCCTGTATTACCCCGGCCATCGCCACGTCCCCGCTGGTCTTCGCGCCTTCATCGACGTTCTGC
GGCACCTGCACTGA

Upstream 100 bases:

>100_bases
TTGGACGGGGCCGCCCGATGCGGCCATCGATGAATCAGTTAGGCGGAAGACATTCGTGCTACTATCCGCGCAATCTTGGA
TGATCTGGTGAAAAATTCCC

Downstream 100 bases:

>100_bases
ACGTATCGGCGTGCGGAAACCGTGCATGGCGGCGTTGGCGCGGTAGGAAGCCGGGGCGGAATTTTGCTATGCATCGGGTC
ATGACCATCCACCCCGACAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNAKPDLSDLTAFAAIAAHRSFRKAANDLGVSASTLSHIMRTLEANLGVRLLHRTTRSVAPTEIGERLLGRLNPVLRDFD
AALQDLEAWRSRPSGTLRINAAEGAVRLLLQTVVPAFFARHPEMALDLVTEARLVDIVAEGFDAGIRIGEAVPRDMVAVR
FGGAIRFVAVASPGYLAAHPPPRTPDDLVHHACIRFRLKSGQLFRWEFARHGQELAVDVAGPLTLDHVELMVEAAADGLG
IAYVPDRTAQPYLSRGAVVGVLDEWCPKIPGLFLYYPGHRHVPAGLRAFIDVLRHLH

Sequences:

>Translated_297_residues
MNAKPDLSDLTAFAAIAAHRSFRKAANDLGVSASTLSHIMRTLEANLGVRLLHRTTRSVAPTEIGERLLGRLNPVLRDFD
AALQDLEAWRSRPSGTLRINAAEGAVRLLLQTVVPAFFARHPEMALDLVTEARLVDIVAEGFDAGIRIGEAVPRDMVAVR
FGGAIRFVAVASPGYLAAHPPPRTPDDLVHHACIRFRLKSGQLFRWEFARHGQELAVDVAGPLTLDHVELMVEAAADGLG
IAYVPDRTAQPYLSRGAVVGVLDEWCPKIPGLFLYYPGHRHVPAGLRAFIDVLRHLH
>Mature_297_residues
MNAKPDLSDLTAFAAIAAHRSFRKAANDLGVSASTLSHIMRTLEANLGVRLLHRTTRSVAPTEIGERLLGRLNPVLRDFD
AALQDLEAWRSRPSGTLRINAAEGAVRLLLQTVVPAFFARHPEMALDLVTEARLVDIVAEGFDAGIRIGEAVPRDMVAVR
FGGAIRFVAVASPGYLAAHPPPRTPDDLVHHACIRFRLKSGQLFRWEFARHGQELAVDVAGPLTLDHVELMVEAAADGLG
IAYVPDRTAQPYLSRGAVVGVLDEWCPKIPGLFLYYPGHRHVPAGLRAFIDVLRHLH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=40.4844290657439, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=40.2730375426621, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=32.5342465753425, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=31.5254237288136, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789440, Length=265, Percent_Identity=27.1698113207547, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI145693193, Length=285, Percent_Identity=31.5789473684211, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789639, Length=287, Percent_Identity=26.4808362369338, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790011, Length=277, Percent_Identity=27.4368231046931, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=27.681660899654, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788706, Length=258, Percent_Identity=27.5193798449612, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789173, Length=267, Percent_Identity=25.0936329588015, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=275, Percent_Identity=24, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=122, Percent_Identity=32.7868852459016, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790399, Length=140, Percent_Identity=33.5714285714286, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32463; Mature: 32463

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKPDLSDLTAFAAIAAHRSFRKAANDLGVSASTLSHIMRTLEANLGVRLLHRTTRSVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PTEIGERLLGRLNPVLRDFDAALQDLEAWRSRPSGTLRINAAEGAVRLLLQTVVPAFFAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHC
HPEMALDLVTEARLVDIVAEGFDAGIRIGEAVPRDMVAVRFGGAIRFVAVASPGYLAAHP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEECCEEEEEEEECCCEEECCC
PPRTPDDLVHHACIRFRLKSGQLFRWEFARHGQELAVDVAGPLTLDHVELMVEAAADGLG
CCCCHHHHHHHHHHHEEECCCCEEEEHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCE
IAYVPDRTAQPYLSRGAVVGVLDEWCPKIPGLFLYYPGHRHVPAGLRAFIDVLRHLH
EEECCCCCCCHHHHCCCEEEHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAKPDLSDLTAFAAIAAHRSFRKAANDLGVSASTLSHIMRTLEANLGVRLLHRTTRSVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PTEIGERLLGRLNPVLRDFDAALQDLEAWRSRPSGTLRINAAEGAVRLLLQTVVPAFFAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHC
HPEMALDLVTEARLVDIVAEGFDAGIRIGEAVPRDMVAVRFGGAIRFVAVASPGYLAAHP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEECCEEEEEEEECCCEEECCC
PPRTPDDLVHHACIRFRLKSGQLFRWEFARHGQELAVDVAGPLTLDHVELMVEAAADGLG
CCCCHHHHHHHHHHHEEECCCCEEEEHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCE
IAYVPDRTAQPYLSRGAVVGVLDEWCPKIPGLFLYYPGHRHVPAGLRAFIDVLRHLH
EEECCCCCCCHHHHCCCEEEHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]