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The map label for this gene is sigK [H]

Identifier: 86747612

GI number: 86747612

Start: 544592

End: 545179

Strand: Direct

Name: sigK [H]

Synonym: RPB_0486

Alternate gene names: 86747612

Gene position: 544592-545179 (Clockwise)

Preceding gene: 86747611

Following gene: 86747613

Centisome position: 10.21

GC content: 66.33

Gene sequence:

>588_bases
ATGGATCATCCGCGACGCAAGCCGCCTGCCGTTTCGCAGGATTTGTCCGACTGCATCGTTCGGATCGCCACCGATCACGA
CGAGGCCGCGTTCGACGTGTTGTTCAGGCACTATGCGCCGCGGCTGAAATCCTACTTCACCAGGCGCGGCGGCGATCCGG
CGATGGCGGAGGAGATCACCCAGGAGACCATGGTCTCGGTCTGGAAGAACGCCGCGCAGTTCGACCCGGCCAAGGCCTCG
GCGACGACCTGGATCTTCACCATCGCCCGCAATCTCGGCATCGACCGGCTGCGGCGGGCGAAGCGGCCGTCGTTCGACCC
GCACGATCCGGCGTTCGTTCCCGACGAGGAGGACCCGCCGGACCGCCAGTTCGAGCGCGCCGAAGCGCAGCGCCGGGTGC
GCGACGTGATGAATACGCTGTCGTCCAACGAGAAGAGCGTTCTGATGTTGTCGTTCTACGAAAATCAATCGCACGGCGAG
ATCGCCCAACGGCTCGGCCTGCCGGTCGGGACGGTGAAGTCGCGGATCCGGCTGGCGTTCGGCAAGCTGCGCGCCGCGCT
CGAGCCGCTGACGGGAGCCGGACGATGA

Upstream 100 bases:

>100_bases
GCTGTGCCCGGCGCGATCTGACGCTAGGCTGCCGGGATCGGACGGGACCTCGCGCAACGTCGCTGTCGATGGTCGTTGGC
CCGGGATTGGACTGCTCGTC

Downstream 100 bases:

>100_bases
CGGTGCGGCATCACATCGGCGACGATCTGCTGCTGAGCTACGCGGCCGGCACGCTGGACGAAGCCTCGAGCCTGCTGGTC
GCCACCCATCTCGCGCTGTG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-K factor [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MDHPRRKPPAVSQDLSDCIVRIATDHDEAAFDVLFRHYAPRLKSYFTRRGGDPAMAEEITQETMVSVWKNAAQFDPAKAS
ATTWIFTIARNLGIDRLRRAKRPSFDPHDPAFVPDEEDPPDRQFERAEAQRRVRDVMNTLSSNEKSVLMLSFYENQSHGE
IAQRLGLPVGTVKSRIRLAFGKLRAALEPLTGAGR

Sequences:

>Translated_195_residues
MDHPRRKPPAVSQDLSDCIVRIATDHDEAAFDVLFRHYAPRLKSYFTRRGGDPAMAEEITQETMVSVWKNAAQFDPAKAS
ATTWIFTIARNLGIDRLRRAKRPSFDPHDPAFVPDEEDPPDRQFERAEAQRRVRDVMNTLSSNEKSVLMLSFYENQSHGE
IAQRLGLPVGTVKSRIRLAFGKLRAALEPLTGAGR
>Mature_195_residues
MDHPRRKPPAVSQDLSDCIVRIATDHDEAAFDVLFRHYAPRLKSYFTRRGGDPAMAEEITQETMVSVWKNAAQFDPAKAS
ATTWIFTIARNLGIDRLRRAKRPSFDPHDPAFVPDEEDPPDRQFERAEAQRRVRDVMNTLSSNEKSVLMLSFYENQSHGE
IAQRLGLPVGTVKSRIRLAFGKLRAALEPLTGAGR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 22074; Mature: 22074

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHPRRKPPAVSQDLSDCIVRIATDHDEAAFDVLFRHYAPRLKSYFTRRGGDPAMAEEIT
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QETMVSVWKNAAQFDPAKASATTWIFTIARNLGIDRLRRAKRPSFDPHDPAFVPDEEDPP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCC
DRQFERAEAQRRVRDVMNTLSSNEKSVLMLSFYENQSHGEIAQRLGLPVGTVKSRIRLAF
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHH
GKLRAALEPLTGAGR
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MDHPRRKPPAVSQDLSDCIVRIATDHDEAAFDVLFRHYAPRLKSYFTRRGGDPAMAEEIT
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QETMVSVWKNAAQFDPAKASATTWIFTIARNLGIDRLRRAKRPSFDPHDPAFVPDEEDPP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCC
DRQFERAEAQRRVRDVMNTLSSNEKSVLMLSFYENQSHGEIAQRLGLPVGTVKSRIRLAF
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHH
GKLRAALEPLTGAGR
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]