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The map label for this gene is cysK [H]

Identifier: 86747603

GI number: 86747603

Start: 525292

End: 526290

Strand: Reverse

Name: cysK [H]

Synonym: RPB_0477

Alternate gene names: 86747603

Gene position: 526290-525292 (Counterclockwise)

Preceding gene: 86747609

Following gene: 86747601

Centisome position: 9.87

GC content: 67.27

Gene sequence:

>999_bases
ATGTCCAGCGCAGCAACCGCATCGCTCAAATCCACCGCCGCAGCGCCCGCCCATCAGCCCGGCCGCGGCCGGGTGTATGA
TTCGGTCGCCGATGCCTATGGCGACACCCCGTTGGTGCGGCTGAACCGGCTGCCGGGACTGAACGGCGTCAACGCGACGA
TTCTCGCCAAGCTGGAATATTTCAACCCGGCCTCCAGCGTGAAGGACCGCATCGGCGCCGCGATGATCGCCGCGATGGAG
CGCGACGGCATCATCAAGCCCGGCACCATCCTGATCGAGCCGACCTCCGGCAACACCGGCATCGCGCTGGCCTATGTGGC
CGCCGCCAAGGGCTATCGGCTCAAGCTGGTGATGCCGGAATCGATGTCGATCGAGCGCCGCAAGATGCTGGCCTTCCTCG
GCGCCGAGCTGGTGCTGACCGAAGCCGCCAAGGGCATGAAGGGCGCCATCGCCAAGGCCGAGGAGCTGATCGCCTCGACG
CCGAACGCGGTGATGCCGCAGCAGTTCAAGAACCTCGCCAACCCCGAGGTTCACCGCCGCACCACCGCCGAGGAGATCTG
GAACGACACCAACGGCGCGATCGACATTTTCGTCGCCGGCGTCGGCACCGGCGGCACCATCACCGGCGTCGGCCAGGTGC
TGAAGCCGCGCAAGCCGTCGGTCAGGATCGTGGCGGTCGAGCCGGAGGAAAGCCCGGTGCTGTCCGGCGGCGCACCCGGC
CCGCACAAGATCCAGGGCATCGGCGCCGGCTTCGTGCCGGACATTCTCGACCGCTCGGTGATCGACGAAATCATCAAGGT
GGCGGGACCGGTTGCGATCGAGACTTCGCGGGCGCTGGCGCGGCACGAAGGCATTCCGGGCGGCATCTCGTCGGGTGCCG
CGATTGCGGCTGCGATCGAACTCGGCAAGCGCCCGGAAAACGCCGGCAAGACCATCGTGGCGATCGTGCCGTCGTTCTCG
GAGCGCTATCTGTCGACCGCGTTGTTCGAGGGCGTGTAA

Upstream 100 bases:

>100_bases
GTTCCATATTTACCAGCCGCGTTGACGGCGGCGGGCGCCGCATATCAGATGAGTAGCAAATCCCGACGATTTCGACCCGA
ATTCCAGCCCGGAGGCGACC

Downstream 100 bases:

>100_bases
CCGCCGCCCACAACCGGCGTCGTCACGCCGTCACTCTGCCGCCGCGAGCGATCGCGGCAGCAGGCCGGCGTCGCGGGCGA
ATTCGACCATGATGTCGCGG

Product: cysteine synthase

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSSAATASLKSTAAAPAHQPGRGRVYDSVADAYGDTPLVRLNRLPGLNGVNATILAKLEYFNPASSVKDRIGAAMIAAME
RDGIIKPGTILIEPTSGNTGIALAYVAAAKGYRLKLVMPESMSIERRKMLAFLGAELVLTEAAKGMKGAIAKAEELIAST
PNAVMPQQFKNLANPEVHRRTTAEEIWNDTNGAIDIFVAGVGTGGTITGVGQVLKPRKPSVRIVAVEPEESPVLSGGAPG
PHKIQGIGAGFVPDILDRSVIDEIIKVAGPVAIETSRALARHEGIPGGISSGAAIAAAIELGKRPENAGKTIVAIVPSFS
ERYLSTALFEGV

Sequences:

>Translated_332_residues
MSSAATASLKSTAAAPAHQPGRGRVYDSVADAYGDTPLVRLNRLPGLNGVNATILAKLEYFNPASSVKDRIGAAMIAAME
RDGIIKPGTILIEPTSGNTGIALAYVAAAKGYRLKLVMPESMSIERRKMLAFLGAELVLTEAAKGMKGAIAKAEELIAST
PNAVMPQQFKNLANPEVHRRTTAEEIWNDTNGAIDIFVAGVGTGGTITGVGQVLKPRKPSVRIVAVEPEESPVLSGGAPG
PHKIQGIGAGFVPDILDRSVIDEIIKVAGPVAIETSRALARHEGIPGGISSGAAIAAAIELGKRPENAGKTIVAIVPSFS
ERYLSTALFEGV
>Mature_331_residues
SSAATASLKSTAAAPAHQPGRGRVYDSVADAYGDTPLVRLNRLPGLNGVNATILAKLEYFNPASSVKDRIGAAMIAAMER
DGIIKPGTILIEPTSGNTGIALAYVAAAKGYRLKLVMPESMSIERRKMLAFLGAELVLTEAAKGMKGAIAKAEELIASTP
NAVMPQQFKNLANPEVHRRTTAEEIWNDTNGAIDIFVAGVGTGGTITGVGQVLKPRKPSVRIVAVEPEESPVLSGGAPGP
HKIQGIGAGFVPDILDRSVIDEIIKVAGPVAIETSRALARHEGIPGGISSGAAIAAAIELGKRPENAGKTIVAIVPSFSE
RYLSTALFEGV

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=322, Percent_Identity=39.7515527950311, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI295821200, Length=322, Percent_Identity=39.7515527950311, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI4557415, Length=322, Percent_Identity=39.7515527950311, Blast_Score=228, Evalue=7e-60,
Organism=Escherichia coli, GI1788754, Length=315, Percent_Identity=56.8253968253968, Blast_Score=311, Evalue=3e-86,
Organism=Escherichia coli, GI2367138, Length=302, Percent_Identity=43.3774834437086, Blast_Score=220, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17535051, Length=297, Percent_Identity=54.2087542087542, Blast_Score=313, Evalue=6e-86,
Organism=Caenorhabditis elegans, GI17562970, Length=309, Percent_Identity=51.4563106796116, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI115535073, Length=312, Percent_Identity=54.1666666666667, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI32566674, Length=301, Percent_Identity=52.1594684385382, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17561720, Length=306, Percent_Identity=50, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI32566672, Length=215, Percent_Identity=52.5581395348837, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17534315, Length=327, Percent_Identity=40.0611620795107, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25147552, Length=323, Percent_Identity=37.4613003095975, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=50.8474576271186, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71996324, Length=332, Percent_Identity=27.710843373494, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=38.7301587301587, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321449, Length=330, Percent_Identity=34.8484848484849, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24643623, Length=312, Percent_Identity=38.4615384615385, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI20129101, Length=312, Percent_Identity=38.4615384615385, Blast_Score=197, Evalue=8e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34430; Mature: 34299

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAATASLKSTAAAPAHQPGRGRVYDSVADAYGDTPLVRLNRLPGLNGVNATILAKLEY
CCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEC
FNPASSVKDRIGAAMIAAMERDGIIKPGTILIEPTSGNTGIALAYVAAAKGYRLKLVMPE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCC
SMSIERRKMLAFLGAELVLTEAAKGMKGAIAKAEELIASTPNAVMPQQFKNLANPEVHRR
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHH
TTAEEIWNDTNGAIDIFVAGVGTGGTITGVGQVLKPRKPSVRIVAVEPEESPVLSGGAPG
HHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
PHKIQGIGAGFVPDILDRSVIDEIIKVAGPVAIETSRALARHEGIPGGISSGAAIAAAIE
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHH
LGKRPENAGKTIVAIVPSFSERYLSTALFEGV
HCCCCCCCCCEEEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSAATASLKSTAAAPAHQPGRGRVYDSVADAYGDTPLVRLNRLPGLNGVNATILAKLEY
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEC
FNPASSVKDRIGAAMIAAMERDGIIKPGTILIEPTSGNTGIALAYVAAAKGYRLKLVMPE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCC
SMSIERRKMLAFLGAELVLTEAAKGMKGAIAKAEELIASTPNAVMPQQFKNLANPEVHRR
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHH
TTAEEIWNDTNGAIDIFVAGVGTGGTITGVGQVLKPRKPSVRIVAVEPEESPVLSGGAPG
HHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
PHKIQGIGAGFVPDILDRSVIDEIIKVAGPVAIETSRALARHEGIPGGISSGAAIAAAIE
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHH
LGKRPENAGKTIVAIVPSFSERYLSTALFEGV
HCCCCCCCCCEEEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]