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The map label for this gene is gatA [H]

Identifier: 86747346

GI number: 86747346

Start: 248432

End: 249847

Strand: Reverse

Name: gatA [H]

Synonym: RPB_0220

Alternate gene names: 86747346

Gene position: 249847-248432 (Counterclockwise)

Preceding gene: 86747347

Following gene: 86747345

Centisome position: 4.69

GC content: 69.35

Gene sequence:

>1416_bases
ATGAGCACGACCGATCCGGCATTGCTGTCGCTGACCGAAATCGCGCAGGCGATCGCTGCGAAGACAGTCTCCTCGCGCGA
GGTGACGCAGGCCTGCCTCGATCGCATCGCGGCCCAGCAGCCGCGGCTCAACGCCTTCATGTCGATCGAAGCCGACAGCG
CGCTCGCCGCCGCGGATGCCGCAGACGCGGAGCTGGCGCGCGGCGACAGTCGCGGCGTGCTGCACGGCGTGCCGCTCGCG
CACAAGGACATGTACTACGAGACGGGTCACGTCGTGACCTGCGGCTCGAAGATCCGGCAGGACGACGTCGCGACCACGAC
CTCGACCGCGCTGCAGCGGCTGAAGGATGCCGGCACGGTTCGGCTGGGCTCGTTGCAGATGTCGGAATTCGCCTATGGGC
CGACCGGCCACAACGCGCATTTCGGCCCGGTGCATAATCCTTGGAACATCGATCACATCACCGGCGGTTCGTCGTCGGGC
TCCGGTGCGGCCGTCGCGGCGCGGCTGACGTTTGCGGCGCTCGGCTCCGACACCGGAGGATCGATCCGGATGCCGGCGCA
TTTCTGCGGCGTCACCGGGCTGAAGACCACGGTCGGCCGCGTCAGCCGCGCCGGCGCGATGCCGCTGTCGCAGTCGCTCG
ACACCGTCGGGCCGCTGGCGCGCACCGCGCAGGATTGCGCGCTCCTGCTCGGGCTGATGGCCGGGGCCGACCCGGAGGAT
CTGACCTGCATCGCCGGCGAACTGCCGGACTATCTCGCGGCGACGCACGCGCCGATGCGCGGCGCGCGGATCGGGATTCC
GAAGGCGTTCTATGTCGACGACCTCGATCCTGAAGTGGCGCGCGTGCTCGACGAGACCATCGCCACGCTCAGGGCCGAAG
GCGCCAGCGTGGTCGAGGTCGATCTGCCCGACCAGCCGCAATTGACCGCGGCGTGCCAGCTTGTGCTCGCGGTGGAAGCC
GCCGCGTTCCACAAGCGCTGGATGATCGAACGGCCGCAGGATTACGGCCCGCAGGTGCTGATGCGGCTGCAGAACGGCCT
CGGCGTGCCGGCGATTTCCTATCTCGAGGCGATGCGCTGGCGCGGGCCGGCACTGTCGGCGCATCTCGCCGCGGTCGCCG
ATGTCGATGCCGTGCTGGCGCCGGTGGCTCCGGTGCCGGCCCCGACGATCGCGGAGAGCGACGTCGGTAGCAGCCTGAAC
GCCGAAGCGGTGATCCAGCGGCTGACGCGCTTCACCCGGCCGATCAACTATCTGGGCCTGCCGTCGCTGGCGATTCCGAC
GGGCTTCAGCGCCAGCGGCCTGCCGATCGGGCTGCAACTGATCGGGCGCCCGTTCGACGAGGCGATGATCCTGCGGATCG
GCGCGGCGTTCCAGCGCGTCACTGAATTCCACAACAAGATGCCGGCGTCAGCATGA

Upstream 100 bases:

>100_bases
TCGGGCCGGCGTTCGACGGCTTCGCGCCGATTGCAGGCTCGCTGCCGTTCGATCTCGAACCCGCGACCTTCCTGGTCGCG
CAGACCGGCAAGGCCGCATC

Downstream 100 bases:

>100_bases
GCAATCTGGTCGAGGTTCGCGATCTCAACATCCGCTTCACCGGCGAGCGCACGGTGCACGCGGTCAACAATCTCAGCCTC
TCGCTCGGCGAAGGCGAGGT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSTTDPALLSLTEIAQAIAAKTVSSREVTQACLDRIAAQQPRLNAFMSIEADSALAAADAADAELARGDSRGVLHGVPLA
HKDMYYETGHVVTCGSKIRQDDVATTTSTALQRLKDAGTVRLGSLQMSEFAYGPTGHNAHFGPVHNPWNIDHITGGSSSG
SGAAVAARLTFAALGSDTGGSIRMPAHFCGVTGLKTTVGRVSRAGAMPLSQSLDTVGPLARTAQDCALLLGLMAGADPED
LTCIAGELPDYLAATHAPMRGARIGIPKAFYVDDLDPEVARVLDETIATLRAEGASVVEVDLPDQPQLTAACQLVLAVEA
AAFHKRWMIERPQDYGPQVLMRLQNGLGVPAISYLEAMRWRGPALSAHLAAVADVDAVLAPVAPVPAPTIAESDVGSSLN
AEAVIQRLTRFTRPINYLGLPSLAIPTGFSASGLPIGLQLIGRPFDEAMILRIGAAFQRVTEFHNKMPASA

Sequences:

>Translated_471_residues
MSTTDPALLSLTEIAQAIAAKTVSSREVTQACLDRIAAQQPRLNAFMSIEADSALAAADAADAELARGDSRGVLHGVPLA
HKDMYYETGHVVTCGSKIRQDDVATTTSTALQRLKDAGTVRLGSLQMSEFAYGPTGHNAHFGPVHNPWNIDHITGGSSSG
SGAAVAARLTFAALGSDTGGSIRMPAHFCGVTGLKTTVGRVSRAGAMPLSQSLDTVGPLARTAQDCALLLGLMAGADPED
LTCIAGELPDYLAATHAPMRGARIGIPKAFYVDDLDPEVARVLDETIATLRAEGASVVEVDLPDQPQLTAACQLVLAVEA
AAFHKRWMIERPQDYGPQVLMRLQNGLGVPAISYLEAMRWRGPALSAHLAAVADVDAVLAPVAPVPAPTIAESDVGSSLN
AEAVIQRLTRFTRPINYLGLPSLAIPTGFSASGLPIGLQLIGRPFDEAMILRIGAAFQRVTEFHNKMPASA
>Mature_470_residues
STTDPALLSLTEIAQAIAAKTVSSREVTQACLDRIAAQQPRLNAFMSIEADSALAAADAADAELARGDSRGVLHGVPLAH
KDMYYETGHVVTCGSKIRQDDVATTTSTALQRLKDAGTVRLGSLQMSEFAYGPTGHNAHFGPVHNPWNIDHITGGSSSGS
GAAVAARLTFAALGSDTGGSIRMPAHFCGVTGLKTTVGRVSRAGAMPLSQSLDTVGPLARTAQDCALLLGLMAGADPEDL
TCIAGELPDYLAATHAPMRGARIGIPKAFYVDDLDPEVARVLDETIATLRAEGASVVEVDLPDQPQLTAACQLVLAVEAA
AFHKRWMIERPQDYGPQVLMRLQNGLGVPAISYLEAMRWRGPALSAHLAAVADVDAVLAPVAPVPAPTIAESDVGSSLNA
EAVIQRLTRFTRPINYLGLPSLAIPTGFSASGLPIGLQLIGRPFDEAMILRIGAAFQRVTEFHNKMPASA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=31.9444444444444, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI195972892, Length=504, Percent_Identity=27.3809523809524, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI166795287, Length=242, Percent_Identity=28.9256198347107, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=417, Percent_Identity=30.6954436450839, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=24.7011952191235, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=302, Percent_Identity=26.4900662251656, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=314, Percent_Identity=28.9808917197452, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=314, Percent_Identity=28.9808917197452, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=247, Percent_Identity=27.9352226720648, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=315, Percent_Identity=23.1746031746032, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=466, Percent_Identity=26.6094420600858, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319685, Length=413, Percent_Identity=27.1186440677966, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=240, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648113, Length=498, Percent_Identity=32.5301204819277, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24652985, Length=516, Percent_Identity=26.1627906976744, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19922090, Length=516, Percent_Identity=26.1627906976744, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24652981, Length=516, Percent_Identity=26.1627906976744, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24652983, Length=516, Percent_Identity=26.1627906976744, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24644968, Length=503, Percent_Identity=25.844930417495, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356731, Length=491, Percent_Identity=24.6435845213849, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161078093, Length=493, Percent_Identity=26.369168356998, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45550774, Length=238, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648435, Length=238, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648437, Length=238, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648441, Length=170, Percent_Identity=30.5882352941176, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648439, Length=170, Percent_Identity=30.5882352941176, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49335; Mature: 49204

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTDPALLSLTEIAQAIAAKTVSSREVTQACLDRIAAQQPRLNAFMSIEADSALAAADA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCC
ADAELARGDSRGVLHGVPLAHKDMYYETGHVVTCGSKIRQDDVATTTSTALQRLKDAGTV
CHHHHHCCCCCCCEECCCCCCCCCEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCE
RLGSLQMSEFAYGPTGHNAHFGPVHNPWNIDHITGGSSSGSGAAVAARLTFAALGSDTGG
EECCEEHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCC
SIRMPAHFCGVTGLKTTVGRVSRAGAMPLSQSLDTVGPLARTAQDCALLLGLMAGADPED
CEECCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LTCIAGELPDYLAATHAPMRGARIGIPKAFYVDDLDPEVARVLDETIATLRAEGASVVEV
CEEEHHCCHHHHHHHCCCCCCCEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEE
DLPDQPQLTAACQLVLAVEAAAFHKRWMIERPQDYGPQVLMRLQNGLGVPAISYLEAMRW
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHC
RGPALSAHLAAVADVDAVLAPVAPVPAPTIAESDVGSSLNAEAVIQRLTRFTRPINYLGL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
PSLAIPTGFSASGLPIGLQLIGRPFDEAMILRIGAAFQRVTEFHNKMPASA
CCHHCCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STTDPALLSLTEIAQAIAAKTVSSREVTQACLDRIAAQQPRLNAFMSIEADSALAAADA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCC
ADAELARGDSRGVLHGVPLAHKDMYYETGHVVTCGSKIRQDDVATTTSTALQRLKDAGTV
CHHHHHCCCCCCCEECCCCCCCCCEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCE
RLGSLQMSEFAYGPTGHNAHFGPVHNPWNIDHITGGSSSGSGAAVAARLTFAALGSDTGG
EECCEEHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCC
SIRMPAHFCGVTGLKTTVGRVSRAGAMPLSQSLDTVGPLARTAQDCALLLGLMAGADPED
CEECCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LTCIAGELPDYLAATHAPMRGARIGIPKAFYVDDLDPEVARVLDETIATLRAEGASVVEV
CEEEHHCCHHHHHHHCCCCCCCEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEE
DLPDQPQLTAACQLVLAVEAAAFHKRWMIERPQDYGPQVLMRLQNGLGVPAISYLEAMRW
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHC
RGPALSAHLAAVADVDAVLAPVAPVPAPTIAESDVGSSLNAEAVIQRLTRFTRPINYLGL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
PSLAIPTGFSASGLPIGLQLIGRPFDEAMILRIGAAFQRVTEFHNKMPASA
CCHHCCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA