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The map label for this gene is nodW [H]

Identifier: 86747337

GI number: 86747337

Start: 237384

End: 238070

Strand: Reverse

Name: nodW [H]

Synonym: RPB_0211

Alternate gene names: 86747337

Gene position: 238070-237384 (Counterclockwise)

Preceding gene: 86747338

Following gene: 86747328

Centisome position: 4.47

GC content: 59.53

Gene sequence:

>687_bases
ATGAGCAAATCTCCGAACCTCTCGCAATCGCCGCTGATCTGCGTTGTCGACGACGATGAGGATGTCCGTAGTTCGCTCAG
CAGCCTGCTACGGTCGATCGGTTATGCGGTGAAGACATTCGCTGGGCCGCTGGATTTTCTCGCCTCGGACGCGGTCGATG
CCGCTTCCTGTCTGATTCTCGATATTCAGCTCGGGGAAGCCGACGGACTTGAATTCCAGCAGGAATTGGTCACGAGCGAC
GCATCGATCCCAATCATCCTGATCTCCGGACACGGCGATATCCCGAAGACGGTGCGCGCGATGAAAGCGGGCGCCGTCAA
TTTTCTACCCAAACCGTTCGAGGAAGAAGCCCTTCTTGCCGCTGTGGCAGAAGCTGTTTCCCGCGACCAGCGTCGACGCA
ACGATGCGCATCGCGATGAGAGCATCCGCCTGCGCTACGAACAGCTCACGCCGCGTGAACGCGAGGTGATGGCACTGGTC
GCAGCCGGGCTGATGAACAAGCAGATCGCCTGGCGGCTGTCGCTCAGCGAAATCACCGTCAAGATTCATCGTGGCAACAT
GATCCGGAAGATGCAGGTGAAATCCGTTGCCGAGCTGGTTCGTCTTGCCGATCAGCTCGGCGTCCGTGCGGCCCCTGAGA
CGCATCCCGATGCAACGCCGAGCCGGCAGGGAAATCCACCGCTTTAG

Upstream 100 bases:

>100_bases
ATAAACCGGTCGATCCGGAAATCCTTCTGCACGACGTCGAGGCGATCCTGCGGGCCGCACGGCATCGCCGCGTCCCGTCC
GACCGATCAGGTCCAAAGTC

Downstream 100 bases:

>100_bases
AGCGGGATGACGGATCTTCGAATCATCATCCTGCTGTGGAGCCTTGTTTGAGCATGATCTCGCGCTCCCGCATGAACGCC
TGAGCAAGCTGCGCGTTCGC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSKSPNLSQSPLICVVDDDEDVRSSLSSLLRSIGYAVKTFAGPLDFLASDAVDAASCLILDIQLGEADGLEFQQELVTSD
ASIPIILISGHGDIPKTVRAMKAGAVNFLPKPFEEEALLAAVAEAVSRDQRRRNDAHRDESIRLRYEQLTPREREVMALV
AAGLMNKQIAWRLSLSEITVKIHRGNMIRKMQVKSVAELVRLADQLGVRAAPETHPDATPSRQGNPPL

Sequences:

>Translated_228_residues
MSKSPNLSQSPLICVVDDDEDVRSSLSSLLRSIGYAVKTFAGPLDFLASDAVDAASCLILDIQLGEADGLEFQQELVTSD
ASIPIILISGHGDIPKTVRAMKAGAVNFLPKPFEEEALLAAVAEAVSRDQRRRNDAHRDESIRLRYEQLTPREREVMALV
AAGLMNKQIAWRLSLSEITVKIHRGNMIRKMQVKSVAELVRLADQLGVRAAPETHPDATPSRQGNPPL
>Mature_227_residues
SKSPNLSQSPLICVVDDDEDVRSSLSSLLRSIGYAVKTFAGPLDFLASDAVDAASCLILDIQLGEADGLEFQQELVTSDA
SIPIILISGHGDIPKTVRAMKAGAVNFLPKPFEEEALLAAVAEAVSRDQRRRNDAHRDESIRLRYEQLTPREREVMALVA
AGLMNKQIAWRLSLSEITVKIHRGNMIRKMQVKSVAELVRLADQLGVRAAPETHPDATPSRQGNPPL

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=30.1587301587302, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=32.8282828282828, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=25.3588516746411, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1786747, Length=205, Percent_Identity=25.3658536585366, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24917; Mature: 24786

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSPNLSQSPLICVVDDDEDVRSSLSSLLRSIGYAVKTFAGPLDFLASDAVDAASCLIL
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEE
DIQLGEADGLEFQQELVTSDASIPIILISGHGDIPKTVRAMKAGAVNFLPKPFEEEALLA
EEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
AVAEAVSRDQRRRNDAHRDESIRLRYEQLTPREREVMALVAAGLMNKQIAWRLSLSEITV
HHHHHHHHHHHHHHHCCCCCHHEEEHHHCCCHHHHHHHHHHHHHHCCHHHEEEEHHHEEE
KIHRGNMIRKMQVKSVAELVRLADQLGVRAAPETHPDATPSRQGNPPL
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKSPNLSQSPLICVVDDDEDVRSSLSSLLRSIGYAVKTFAGPLDFLASDAVDAASCLIL
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEE
DIQLGEADGLEFQQELVTSDASIPIILISGHGDIPKTVRAMKAGAVNFLPKPFEEEALLA
EEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
AVAEAVSRDQRRRNDAHRDESIRLRYEQLTPREREVMALVAAGLMNKQIAWRLSLSEITV
HHHHHHHHHHHHHHHCCCCCHHEEEHHHCCCHHHHHHHHHHHHHHCCHHHEEEEHHHEEE
KIHRGNMIRKMQVKSVAELVRLADQLGVRAAPETHPDATPSRQGNPPL
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]