Click here to switch to the map view.

The map label for this gene is rsmB [H]

Identifier: 86747213

GI number: 86747213

Start: 92238

End: 93590

Strand: Reverse

Name: rsmB [H]

Synonym: RPB_0086

Alternate gene names: 86747213

Gene position: 93590-92238 (Counterclockwise)

Preceding gene: 86747216

Following gene: 86747212

Centisome position: 1.76

GC content: 70.58

Gene sequence:

>1353_bases
ATGCCTGCTCCAAGATTCGCACAGCCGTCGGAGGTGCCCGGACTGGCAGCGCGCCGGATCGCCGCCGACATCCTCGACGG
CGTCCTGCAGAAGCGCCGGATGCTGGACGACCAGCTCGAAGGCCCCGCCGCACATCCCGGGCTCAAGACGCTGCCGGATC
GTGACCGCGCGCTGATGCGCCGGCTGGTGGCGACCATTCTGCGCCGGCTCGGCACGCTCGAACATCTGCTGGCGCGGATG
CTCGATCGCGGGGTTCCGACCGATGCGCCGCGCGCCAAGAGCGCGCTCTTGATCGGCGCCGCGCAGATCCTGTGGATGGA
TGTTCCGGACCACGCCGCCGTCGATCTGTCGGTGCGGCTGGTGCAGTCCGACCGCCGTGCCGCCAAATATGCCGGCCTTG
TCAACGCCGTGCTGCGGCGCTGCGCGCGCGAGGGGCATCCCTTGATCGACGCGGTCGCGGGCCAGGCGCTCGACGTGCCG
GAATGGCTGATGGCGCGGTGGCGCGCGCATTACGGCGACGACGCCGCCAAGGCGATCGCGCTTGCGATCAGCCATGAGCC
GTCGCTCGACATCACCGTCAAATCCGATCCCGACCATTGGGCCGGGCGGCTGTATGGCGAATTGCTGCCGACCGGCACGG
TGCGGACGCTGCTGCAGGGCTCGGTGAAGATGCTGCCGGGCTTCGACGACGGCCAATGGTGGGTGCAGGATGCCGCCGCC
GCTTTGCCGGCGCGGCTGTTCGGCGATCTCGCCGGCAAATCGGTGGCCGATCTCTGCGCCGCACCGGGCGGCAAGACCGC
GCAACTGGCGCAGGCCGGCGCGCGGGTCAGCGCGATCGATCGCTCGCCGGCGCGGGTGGCGCGGTTGCGCGAAAATCTGG
CGCGGCTGGGACTCGAGGCCGAGACCGCCGTGGCTGACGCCACCGAATGGCGCAGCGATGCCCCCGGCAGTTTCGACGGC
GTGCTGGTCGACGCCCCCTGCGCCTCGACCGGCACCATCCGCCGGCATCCCGATGTCGCCTGGCTGAAGCAGGAGAGCGA
CATCGGCGCGCTGTCGGCGGTGCAGAGCCGGCTGCTGCACAAGGCGGTCGCGCTGCTCAAGCCGGGCGGCACGCTGGTCT
ATTGCACCTGTTCGCTGGAGCCGGAGGAAGGCGAGCAGGTGGTCGGCGCGCTGTTGGACGCCGAGCCCGGGATGCGGCGC
CTCCCGGTGGAGCCCGCCGAGGTTGCCGGCCTTGCCGAGATCGTGACCGCCGAGGGCGATGTTCGGACCCTGCCGTCGCA
TCTGCCCCATGCTGATCCGAGGCTCGCGGGGTTCGATGGGTTCTACGCCGCCCGTCTTACGAAGTCTTTGTAA

Upstream 100 bases:

>100_bases
GCGGCGATTCTTAGAATCGGCATATTCGCCTGCCATCCATCGCCGAATTCGCGCCGCCTCGCGCGCGGCATCATCCCCTC
CAATGTGATACCCTCAATCC

Downstream 100 bases:

>100_bases
TCATTTTGAAATTGGCAGCCATGGCGCCCCGCCGGGGACGCCGAAACGGTGCCCGTCCACACCCCGCCCAACGGCAGGGT
GTGTGCATGGCCAATTCCGG

Product: RNA methyltransferase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPAPRFAQPSEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLPDRDRALMRRLVATILRRLGTLEHLLARM
LDRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGHPLIDAVAGQALDVP
EWLMARWRAHYGDDAAKAIALAISHEPSLDITVKSDPDHWAGRLYGELLPTGTVRTLLQGSVKMLPGFDDGQWWVQDAAA
ALPARLFGDLAGKSVADLCAAPGGKTAQLAQAGARVSAIDRSPARVARLRENLARLGLEAETAVADATEWRSDAPGSFDG
VLVDAPCASTGTIRRHPDVAWLKQESDIGALSAVQSRLLHKAVALLKPGGTLVYCTCSLEPEEGEQVVGALLDAEPGMRR
LPVEPAEVAGLAEIVTAEGDVRTLPSHLPHADPRLAGFDGFYAARLTKSL

Sequences:

>Translated_450_residues
MPAPRFAQPSEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLPDRDRALMRRLVATILRRLGTLEHLLARM
LDRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGHPLIDAVAGQALDVP
EWLMARWRAHYGDDAAKAIALAISHEPSLDITVKSDPDHWAGRLYGELLPTGTVRTLLQGSVKMLPGFDDGQWWVQDAAA
ALPARLFGDLAGKSVADLCAAPGGKTAQLAQAGARVSAIDRSPARVARLRENLARLGLEAETAVADATEWRSDAPGSFDG
VLVDAPCASTGTIRRHPDVAWLKQESDIGALSAVQSRLLHKAVALLKPGGTLVYCTCSLEPEEGEQVVGALLDAEPGMRR
LPVEPAEVAGLAEIVTAEGDVRTLPSHLPHADPRLAGFDGFYAARLTKSL
>Mature_449_residues
PAPRFAQPSEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLPDRDRALMRRLVATILRRLGTLEHLLARML
DRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGHPLIDAVAGQALDVPE
WLMARWRAHYGDDAAKAIALAISHEPSLDITVKSDPDHWAGRLYGELLPTGTVRTLLQGSVKMLPGFDDGQWWVQDAAAA
LPARLFGDLAGKSVADLCAAPGGKTAQLAQAGARVSAIDRSPARVARLRENLARLGLEAETAVADATEWRSDAPGSFDGV
LVDAPCASTGTIRRHPDVAWLKQESDIGALSAVQSRLLHKAVALLKPGGTLVYCTCSLEPEEGEQVVGALLDAEPGMRRL
PVEPAEVAGLAEIVTAEGDVRTLPSHLPHADPRLAGFDGFYAARLTKSL

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=238, Percent_Identity=33.6134453781513, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI76150623, Length=238, Percent_Identity=33.6134453781513, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI32698918, Length=203, Percent_Identity=31.5270935960591, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI40316918, Length=172, Percent_Identity=35.4651162790698, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI11545785, Length=201, Percent_Identity=29.8507462686567, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI23199998, Length=234, Percent_Identity=32.4786324786325, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI270288816, Length=234, Percent_Identity=32.4786324786325, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI270288818, Length=234, Percent_Identity=32.4786324786325, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI8922322, Length=234, Percent_Identity=32.4786324786325, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI2367212, Length=454, Percent_Identity=30.8370044052863, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI87081985, Length=226, Percent_Identity=33.6283185840708, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17536757, Length=238, Percent_Identity=31.5126050420168, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71998419, Length=229, Percent_Identity=30.5676855895196, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115534021, Length=224, Percent_Identity=29.9107142857143, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25143471, Length=182, Percent_Identity=34.6153846153846, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17509941, Length=178, Percent_Identity=28.0898876404494, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=242, Percent_Identity=31.404958677686, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319447, Length=177, Percent_Identity=33.8983050847458, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI22024126, Length=237, Percent_Identity=31.6455696202532, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24668781, Length=203, Percent_Identity=33.0049261083744, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356513, Length=203, Percent_Identity=33.0049261083744, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356579, Length=185, Percent_Identity=27.027027027027, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48107; Mature: 47976

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPRFAQPSEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLPDRDRALMR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
RLVATILRRLGTLEHLLARMLDRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRL
HHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCEEEHEEEE
VQSDRRAAKYAGLVNAVLRRCAREGHPLIDAVAGQALDVPEWLMARWRAHYGDDAAKAIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHEE
LAISHEPSLDITVKSDPDHWAGRLYGELLPTGTVRTLLQGSVKMLPGFDDGQWWVQDAAA
EEEECCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
ALPARLFGDLAGKSVADLCAAPGGKTAQLAQAGARVSAIDRSPARVARLRENLARLGLEA
HHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCCH
ETAVADATEWRSDAPGSFDGVLVDAPCASTGTIRRHPDVAWLKQESDIGALSAVQSRLLH
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHHH
KAVALLKPGGTLVYCTCSLEPEEGEQVVGALLDAEPGMRRLPVEPAEVAGLAEIVTAEGD
HHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
VRTLPSHLPHADPRLAGFDGFYAARLTKSL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PAPRFAQPSEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLPDRDRALMR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
RLVATILRRLGTLEHLLARMLDRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRL
HHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCEEEHEEEE
VQSDRRAAKYAGLVNAVLRRCAREGHPLIDAVAGQALDVPEWLMARWRAHYGDDAAKAIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHEE
LAISHEPSLDITVKSDPDHWAGRLYGELLPTGTVRTLLQGSVKMLPGFDDGQWWVQDAAA
EEEECCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
ALPARLFGDLAGKSVADLCAAPGGKTAQLAQAGARVSAIDRSPARVARLRENLARLGLEA
HHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCCH
ETAVADATEWRSDAPGSFDGVLVDAPCASTGTIRRHPDVAWLKQESDIGALSAVQSRLLH
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHHH
KAVALLKPGGTLVYCTCSLEPEEGEQVVGALLDAEPGMRRLPVEPAEVAGLAEIVTAEGD
HHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
VRTLPSHLPHADPRLAGFDGFYAARLTKSL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA