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The map label for this gene is hemK [H]

Identifier: 86747199

GI number: 86747199

Start: 74067

End: 74936

Strand: Reverse

Name: hemK [H]

Synonym: RPB_0072

Alternate gene names: 86747199

Gene position: 74936-74067 (Counterclockwise)

Preceding gene: 86747200

Following gene: 86747198

Centisome position: 1.41

GC content: 72.64

Gene sequence:

>870_bases
GTGGATCAACAGAATTCTGTCGCCGAAGCCCGCCGCGCGCTAGGCCGGCGCCTGAAGGACGCCGGCATCGAATCGGCCGA
GCTCGACGCCCGGCTGCTGATCGGCGAGGCGACCGGACTCGATCTCACCGGACTGATCGTGCAGGCCGAGCGGGTGCTCA
CGCTGGACGAGGCGGAGCGGCTCGACGCCTTCGCGGTGCGCCGGTTGGCCGGTGAGCCGGTGGCGCGGATCCTCGGCGTC
CGCGAATTCTGGGGGCTGGCGCTGCGGCTGTCCGACGACACGCTGGTGCCGCGGCCCGACACCGAGACCGTGGTCGAGGC
CGCGCTCGACCATCTGCGCGCCGAGGCGCGCGCCCGCCCGCTGATTCTCGATCTCGGCACCGGCTCCGGCGCGATCCTGC
TGGCGCTGCTGTCGGAGTGCCCGGACGCGTTCGGCGTCGCGACCGACATCAGTCTCGGCGCCTTGCGCGCCGCGCGGGCG
AATGCCGCCGCGCTCGGCCTTGCCGATCGCGCCGGCTTCGTCGCCTGCGACTACGCTGCTGCGCTCGGCGGTTCGTTCGA
CCTGATCGTCTCCAACCCGCCATACATCCCGGCAAGCGCGATCGCCGCGCTCGATGTCGAGGTCCGGGAGCACGATCCGC
GCCGCGCGCTCGACGGCGGTGAGGACGGCCTCGATGCCTATCGCCGGATCATCCCCGAAGCGGCGCGGCTGCTCGGGCGC
GGCGGGGCGTTGGTGGTGGAGATCGGCCAAGGCCAGGGCGACGACGTCGCCGCGCTGATGCGGGCCTCTGGGCTCGCCGT
CCCGGAGCCGCCACGCCGCGATCTGGGTGGCGTTTTTCGGGCGGTGACGGGGCGCAATTTGACGGGTTAA

Upstream 100 bases:

>100_bases
ACTATCAGGTGAATGGCTCGAATAATTTATCTGCGCCACTTAGAAGTTCGGCAAGGCTCATGCATATTGATACCTTGGCA
CGGCGTCGAAGTTGAGACTG

Downstream 100 bases:

>100_bases
TGTCGGAATAGATTGCAAAAAACGCTTGGAATATCGACCGAGAGCGACTACGTTCCGGCCAGGATATCGGTCCAGTCGGC
TGGTCCCGCGGGTCTGCGGT

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDQQNSVAEARRALGRRLKDAGIESAELDARLLIGEATGLDLTGLIVQAERVLTLDEAERLDAFAVRRLAGEPVARILGV
REFWGLALRLSDDTLVPRPDTETVVEAALDHLRAEARARPLILDLGTGSGAILLALLSECPDAFGVATDISLGALRAARA
NAAALGLADRAGFVACDYAAALGGSFDLIVSNPPYIPASAIAALDVEVREHDPRRALDGGEDGLDAYRRIIPEAARLLGR
GGALVVEIGQGQGDDVAALMRASGLAVPEPPRRDLGGVFRAVTGRNLTG

Sequences:

>Translated_289_residues
MDQQNSVAEARRALGRRLKDAGIESAELDARLLIGEATGLDLTGLIVQAERVLTLDEAERLDAFAVRRLAGEPVARILGV
REFWGLALRLSDDTLVPRPDTETVVEAALDHLRAEARARPLILDLGTGSGAILLALLSECPDAFGVATDISLGALRAARA
NAAALGLADRAGFVACDYAAALGGSFDLIVSNPPYIPASAIAALDVEVREHDPRRALDGGEDGLDAYRRIIPEAARLLGR
GGALVVEIGQGQGDDVAALMRASGLAVPEPPRRDLGGVFRAVTGRNLTG
>Mature_289_residues
MDQQNSVAEARRALGRRLKDAGIESAELDARLLIGEATGLDLTGLIVQAERVLTLDEAERLDAFAVRRLAGEPVARILGV
REFWGLALRLSDDTLVPRPDTETVVEAALDHLRAEARARPLILDLGTGSGAILLALLSECPDAFGVATDISLGALRAARA
NAAALGLADRAGFVACDYAAALGGSFDLIVSNPPYIPASAIAALDVEVREHDPRRALDGGEDGLDAYRRIIPEAARLLGR
GGALVVEIGQGQGDDVAALMRASGLAVPEPPRRDLGGVFRAVTGRNLTG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=251, Percent_Identity=34.2629482071713, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1787463, Length=263, Percent_Identity=40.3041825095057, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI87082085, Length=201, Percent_Identity=34.3283582089552, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24582226, Length=254, Percent_Identity=28.3464566929134, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30276; Mature: 30276

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQNSVAEARRALGRRLKDAGIESAELDARLLIGEATGLDLTGLIVQAERVLTLDEAER
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHEEHHHHHEEHHHHHH
LDAFAVRRLAGEPVARILGVREFWGLALRLSDDTLVPRPDTETVVEAALDHLRAEARARP
HHHHHHHHHCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LILDLGTGSGAILLALLSECPDAFGVATDISLGALRAARANAAALGLADRAGFVACDYAA
EEEEECCCCHHHHHHHHHHCCHHHCCHHCCHHHHHHHHHCCHHHHCCCCCCCEEEEHHHH
ALGGSFDLIVSNPPYIPASAIAALDVEVREHDPRRALDGGEDGLDAYRRIIPEAARLLGR
HHCCCEEEEECCCCCCCHHHHHEEEEEHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCC
GGALVVEIGQGQGDDVAALMRASGLAVPEPPRRDLGGVFRAVTGRNLTG
CCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDQQNSVAEARRALGRRLKDAGIESAELDARLLIGEATGLDLTGLIVQAERVLTLDEAER
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHEEHHHHHEEHHHHHH
LDAFAVRRLAGEPVARILGVREFWGLALRLSDDTLVPRPDTETVVEAALDHLRAEARARP
HHHHHHHHHCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LILDLGTGSGAILLALLSECPDAFGVATDISLGALRAARANAAALGLADRAGFVACDYAA
EEEEECCCCHHHHHHHHHHCCHHHCCHHCCHHHHHHHHHCCHHHHCCCCCCCEEEEHHHH
ALGGSFDLIVSNPPYIPASAIAALDVEVREHDPRRALDGGEDGLDAYRRIIPEAARLLGR
HHCCCEEEEECCCCCCCHHHHHEEEEEHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCC
GGALVVEIGQGQGDDVAALMRASGLAVPEPPRRDLGGVFRAVTGRNLTG
CCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]