Click here to switch to the map view.

The map label for this gene is gloB

Identifier: 86747196

GI number: 86747196

Start: 71018

End: 71785

Strand: Reverse

Name: gloB

Synonym: RPB_0069

Alternate gene names: 86747196

Gene position: 71785-71018 (Counterclockwise)

Preceding gene: 86747198

Following gene: 86747195

Centisome position: 1.35

GC content: 66.15

Gene sequence:

>768_bases
ATGGCTGCCGACATCCGCATCGTTCCGTGCCTCACCGACAATTTCGGCTATCTGATCCACGACCCGTCGAGCGGCGCCAC
GGCCTCGATCGACGCGCCCGAGGCCGCGCCGCTGATCGCGGCGCTCGAGAAGGAAGGCTGGAAGCTCACGGATATCCTGG
TGACACATCATCACGGCGATCACGTCGGCGGCATCGCCGAACTGAAGGCGAAGTATCACTGCCGCGTCGTTGCGCCGCAC
GACGCCAACGCCAAGATCGCCGACGCCGATCTGCGGGTCGAGGAGGGCGATGTCGTCAAGGTCGGCGGGCTCGAAGCGCG
GGTGCTGGAAACCCCCGGCCATACGCTCGACCACATCTCCTACGTGTTCGCCGACGACCGCGCGCTGTTCGCCGCCGATA
CGCTGTTCTCGATCGGTTGCGGCCGGGTGTTCGAGGGCACCTATCCGATGATGTGGGAATCGCTGCTGAAGCTGCGGGCG
CTGCCCGACGACTACAAGCTTTATTGCGGCCACGAATACACCGCCTCGAACGTCAAATTCGCGCTGACGATCGAGCCCGA
GAACGCCGCCCTGCAGGCGCGCGCCAAACAGGTCGAGCAGCAGCGCGGCGCCGGCCGGCCGACCATCCCGGTGACGCTCG
GCGAGGAGAAACAGGCCAATGTGTTCCTGCGCGCCGACGTGCCGTCGGTCGCAGCGGCCATTGGTTTCGCCGGCGAGAGC
GCGGCGGACGTGTTCGGTGAACTCCGCGAGCGCAAGAACAACTCGTGA

Upstream 100 bases:

>100_bases
TTCGCCGAACTCGCCGGCCGTGGTAGACTTTGCATTGCGTCGCTCCGGCGGTCGCCACCAGCGTCGGGCGCATCCGCACC
GTCAATTCCCGGGAGATCTC

Downstream 100 bases:

>100_bases
TGCGGGCGACGCTGTCCGCCGCGGAGGTGATCGCGCAACTCGGCCTCGCGCCGCATCCCGAAGGCGGGCACTATCGCGAG
ACGTTTCGCGATTCCCGCTG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAADIRIVPCLTDNFGYLIHDPSSGATASIDAPEAAPLIAALEKEGWKLTDILVTHHHGDHVGGIAELKAKYHCRVVAPH
DANAKIADADLRVEEGDVVKVGGLEARVLETPGHTLDHISYVFADDRALFAADTLFSIGCGRVFEGTYPMMWESLLKLRA
LPDDYKLYCGHEYTASNVKFALTIEPENAALQARAKQVEQQRGAGRPTIPVTLGEEKQANVFLRADVPSVAAAIGFAGES
AADVFGELRERKNNS

Sequences:

>Translated_255_residues
MAADIRIVPCLTDNFGYLIHDPSSGATASIDAPEAAPLIAALEKEGWKLTDILVTHHHGDHVGGIAELKAKYHCRVVAPH
DANAKIADADLRVEEGDVVKVGGLEARVLETPGHTLDHISYVFADDRALFAADTLFSIGCGRVFEGTYPMMWESLLKLRA
LPDDYKLYCGHEYTASNVKFALTIEPENAALQARAKQVEQQRGAGRPTIPVTLGEEKQANVFLRADVPSVAAAIGFAGES
AADVFGELRERKNNS
>Mature_254_residues
AADIRIVPCLTDNFGYLIHDPSSGATASIDAPEAAPLIAALEKEGWKLTDILVTHHHGDHVGGIAELKAKYHCRVVAPHD
ANAKIADADLRVEEGDVVKVGGLEARVLETPGHTLDHISYVFADDRALFAADTLFSIGCGRVFEGTYPMMWESLLKLRAL
PDDYKLYCGHEYTASNVKFALTIEPENAALQARAKQVEQQRGAGRPTIPVTLGEEKQANVFLRADVPSVAAAIGFAGESA
ADVFGELRERKNNS

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI116642887, Length=235, Percent_Identity=39.1489361702128, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI21703352, Length=235, Percent_Identity=39.1489361702128, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI94538320, Length=238, Percent_Identity=37.3949579831933, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI94538322, Length=238, Percent_Identity=37.3949579831933, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI14150041, Length=226, Percent_Identity=35.8407079646018, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=35.3535353535354, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI41327741, Length=183, Percent_Identity=32.7868852459016, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI7705793, Length=135, Percent_Identity=32.5925925925926, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1786406, Length=224, Percent_Identity=41.0714285714286, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1787158, Length=161, Percent_Identity=31.6770186335404, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17536925, Length=251, Percent_Identity=34.2629482071713, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538952, Length=180, Percent_Identity=28.3333333333333, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320478, Length=261, Percent_Identity=33.7164750957854, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324614, Length=267, Percent_Identity=29.5880149812734, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356335, Length=257, Percent_Identity=37.7431906614786, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24667711, Length=257, Percent_Identity=37.3540856031128, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24667703, Length=257, Percent_Identity=37.3540856031128, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI221330176, Length=167, Percent_Identity=34.1317365269461, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_RHOP2 (Q2J429)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483692.1
- HSSP:   Q9C8L4
- ProteinModelPortal:   Q2J429
- SMR:   Q2J429
- STRING:   Q2J429
- GeneID:   3907809
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0069
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK818037
- BioCyc:   RPAL316058:RPB_0069-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 27545; Mature: 27414

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADIRIVPCLTDNFGYLIHDPSSGATASIDAPEAAPLIAALEKEGWKLTDILVTHHHGD
CCCCEEEEEEEECCCCEEEECCCCCCEEECCCCCCCHHHHHHHCCCCEEEEEEEEECCCC
HVGGIAELKAKYHCRVVAPHDANAKIADADLRVEEGDVVKVGGLEARVLETPGHTLDHIS
CCCCHHHCCEEEEEEEECCCCCCCEEECCEEEECCCCEEEECCCEEEEEECCCCHHHHEE
YVFADDRALFAADTLFSIGCGRVFEGTYPMMWESLLKLRALPDDYKLYCGHEYTASNVKF
EEEECCCEEEEHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCCCEEEECCEEECCCEEE
ALTIEPENAALQARAKQVEQQRGAGRPTIPVTLGEEKQANVFLRADVPSVAAAIGFAGES
EEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHCCCCCH
AADVFGELRERKNNS
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
AADIRIVPCLTDNFGYLIHDPSSGATASIDAPEAAPLIAALEKEGWKLTDILVTHHHGD
CCCEEEEEEEECCCCEEEECCCCCCEEECCCCCCCHHHHHHHCCCCEEEEEEEEECCCC
HVGGIAELKAKYHCRVVAPHDANAKIADADLRVEEGDVVKVGGLEARVLETPGHTLDHIS
CCCCHHHCCEEEEEEEECCCCCCCEEECCEEEECCCCEEEECCCEEEEEECCCCHHHHEE
YVFADDRALFAADTLFSIGCGRVFEGTYPMMWESLLKLRALPDDYKLYCGHEYTASNVKF
EEEECCCEEEEHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCCCEEEECCEEECCCEEE
ALTIEPENAALQARAKQVEQQRGAGRPTIPVTLGEEKQANVFLRADVPSVAAAIGFAGES
EEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHCCCCCH
AADVFGELRERKNNS
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA