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The map label for this gene is rpfG [H]

Identifier: 86608396

GI number: 86608396

Start: 953731

End: 954756

Strand: Direct

Name: rpfG [H]

Synonym: CYB_0917

Alternate gene names: 86608396

Gene position: 953731-954756 (Clockwise)

Preceding gene: 86608395

Following gene: 86608398

Centisome position: 31.3

GC content: 60.43

Gene sequence:

>1026_bases
ATGAACTCCCACCTGATCCACACCCCCTTTCCCCGCCTAAACCCGAAGCTGGCGGAGGCGGATCATGTTTTGGTGGTGGA
CGATCAGGCCAGCAGCCGCCTCTACATCGGTGAGCTGTTGCGGTCGGCCCACTTCCGCGTGAGCGAGGCCACCAACGGCG
AAGAGGCTTTGGAGGCTGTGGAGCGGCTTCGGCCAGACTTGGTGCTGTTGGATGTGCTGATGCCGAACATGAGCGGGTTC
GATGTCTGCCGGCAACTGAAGGCCAATGAGCTGACCCGCCTGATCCCCGTTGTGCTGATCACTGCCCTGACCGACCGCTC
CAATCGCCTGATGGGGATGCAGGTGGGGGCCGACGACATTTTGACCAAGCCCTTCGATCACGTGGAGCTGCTGGCGCGGG
TACGCTCATCCATCCACCAAAAGCGCTTGAACGAAGACCTGGATCACGCTGCCGGGGTGTTGTTTGCCATTGCCCGCGCT
GTGGAGAGCCGGGATCCCATCACGGGTGACCATTGCGAACGCTTGGTGGCCATGGGGGAACAGTTTGGCCGTTACTTGGG
GCTGTCTCGTCAACAGATCAAGGCCCTGCGCTGGGGCGGCTACCTGCACGACATCGGCAAAGTCGGGATCCCGGATGCGA
TTTTGTGCAAGCAGGGCAAGCATACCCCGGAAGAATGGGAGATCATGAAATCCCACGTCTTGATTGGCGAAGACATTTGT
CGGGGCTTGCGCACCATGCAGGATGTGCTGCCGATCATCCGCCACCACCACGAGCGTTGGGATGGCAGCGGCTACCCGGA
TGGGCTGGCAGGCGAAGAGATTCCCCTGTTGGCCCGCGTCTTTCAGGTGATCGACATTTTCGACGCTCTGACCTCTCCTC
GCCCCTACAAACCGGCCTTGACCGCCGAACAGGCCCTGGAAGTGCTGCGGGAGGAAACCCGCAAGGGCTGGCGGGATCCG
AACTTGGTGCGCGAGTTTTGTGACTTTATGTTTGTTACGGCCTTTAATTTGCTGGAGGCGGGATAG

Upstream 100 bases:

>100_bases
TCCTACCGCCAGGGGTCTGGGCTGGGATCCCTCGTAGAGTTGCGCCGAATCCGGCTGAGGGGGAAAGCTCGCACTCGATG
CAAGGCTAGGTTTTACCACC

Downstream 100 bases:

>100_bases
GGTCTGGAGGGCCGGGCCAGTCTGGAGAGGCGGCTTTTGGGGTTTGAGCGGCAACGATTACCCCGGCTACACGGATCCCG
GCAGCGGCCAATACCTGCTG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNSHLIHTPFPRLNPKLAEADHVLVVDDQASSRLYIGELLRSAHFRVSEATNGEEALEAVERLRPDLVLLDVLMPNMSGF
DVCRQLKANELTRLIPVVLITALTDRSNRLMGMQVGADDILTKPFDHVELLARVRSSIHQKRLNEDLDHAAGVLFAIARA
VESRDPITGDHCERLVAMGEQFGRYLGLSRQQIKALRWGGYLHDIGKVGIPDAILCKQGKHTPEEWEIMKSHVLIGEDIC
RGLRTMQDVLPIIRHHHERWDGSGYPDGLAGEEIPLLARVFQVIDIFDALTSPRPYKPALTAEQALEVLREETRKGWRDP
NLVREFCDFMFVTAFNLLEAG

Sequences:

>Translated_341_residues
MNSHLIHTPFPRLNPKLAEADHVLVVDDQASSRLYIGELLRSAHFRVSEATNGEEALEAVERLRPDLVLLDVLMPNMSGF
DVCRQLKANELTRLIPVVLITALTDRSNRLMGMQVGADDILTKPFDHVELLARVRSSIHQKRLNEDLDHAAGVLFAIARA
VESRDPITGDHCERLVAMGEQFGRYLGLSRQQIKALRWGGYLHDIGKVGIPDAILCKQGKHTPEEWEIMKSHVLIGEDIC
RGLRTMQDVLPIIRHHHERWDGSGYPDGLAGEEIPLLARVFQVIDIFDALTSPRPYKPALTAEQALEVLREETRKGWRDP
NLVREFCDFMFVTAFNLLEAG
>Mature_341_residues
MNSHLIHTPFPRLNPKLAEADHVLVVDDQASSRLYIGELLRSAHFRVSEATNGEEALEAVERLRPDLVLLDVLMPNMSGF
DVCRQLKANELTRLIPVVLITALTDRSNRLMGMQVGADDILTKPFDHVELLARVRSSIHQKRLNEDLDHAAGVLFAIARA
VESRDPITGDHCERLVAMGEQFGRYLGLSRQQIKALRWGGYLHDIGKVGIPDAILCKQGKHTPEEWEIMKSHVLIGEDIC
RGLRTMQDVLPIIRHHHERWDGSGYPDGLAGEEIPLLARVFQVIDIFDALTSPRPYKPALTAEQALEVLREETRKGWRDP
NLVREFCDFMFVTAFNLLEAG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=37.1900826446281, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788394, Length=128, Percent_Identity=29.6875, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786784, Length=115, Percent_Identity=36.5217391304348, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789809, Length=125, Percent_Identity=31.2, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=35.5932203389831, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082012, Length=148, Percent_Identity=29.0540540540541, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=37.0689655172414, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=129, Percent_Identity=30.2325581395349, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786911, Length=119, Percent_Identity=31.0924369747899, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790863, Length=117, Percent_Identity=29.0598290598291, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787229, Length=181, Percent_Identity=25.9668508287293, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367329, Length=125, Percent_Identity=34.4, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=36.2068965517241, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 38494; Mature: 38494

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSHLIHTPFPRLNPKLAEADHVLVVDDQASSRLYIGELLRSAHFRVSEATNGEEALEAV
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
ERLRPDLVLLDVLMPNMSGFDVCRQLKANELTRLIPVVLITALTDRSNRLMGMQVGADDI
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
LTKPFDHVELLARVRSSIHQKRLNEDLDHAAGVLFAIARAVESRDPITGDHCERLVAMGE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QFGRYLGLSRQQIKALRWGGYLHDIGKVGIPDAILCKQGKHTPEEWEIMKSHVLIGEDIC
HHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RGLRTMQDVLPIIRHHHERWDGSGYPDGLAGEEIPLLARVFQVIDIFDALTSPRPYKPAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
TAEQALEVLREETRKGWRDPNLVREFCDFMFVTAFNLLEAG
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSHLIHTPFPRLNPKLAEADHVLVVDDQASSRLYIGELLRSAHFRVSEATNGEEALEAV
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
ERLRPDLVLLDVLMPNMSGFDVCRQLKANELTRLIPVVLITALTDRSNRLMGMQVGADDI
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
LTKPFDHVELLARVRSSIHQKRLNEDLDHAAGVLFAIARAVESRDPITGDHCERLVAMGE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QFGRYLGLSRQQIKALRWGGYLHDIGKVGIPDAILCKQGKHTPEEWEIMKSHVLIGEDIC
HHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RGLRTMQDVLPIIRHHHERWDGSGYPDGLAGEEIPLLARVFQVIDIFDALTSPRPYKPAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
TAEQALEVLREETRKGWRDPNLVREFCDFMFVTAFNLLEAG
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]