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The map label for this gene is sphS [H]

Identifier: 86608340

GI number: 86608340

Start: 891888

End: 893147

Strand: Direct

Name: sphS [H]

Synonym: CYB_0858

Alternate gene names: 86608340

Gene position: 891888-893147 (Clockwise)

Preceding gene: 86608339

Following gene: 86608341

Centisome position: 29.27

GC content: 58.02

Gene sequence:

>1260_bases
ATGGGACTGGAAGCCGGGATCCTGATCGGGTTGGGAATCGGTTTGCTTTGCACGTTGCTCCTGCTGTGGCAAAGTCGGCG
TCGTTTCAGCCGCGAATTGCAACGGTTGGGATCCCTGGTGCATCTGGCCATGCCCCTCCACTCCCTGGAACAGATGCTCA
GCTTGCTCAGCCGTTATCTTCGCCAACAAGATCTGGATTGGGCCAACCAAAAACAGCAACTGCAAAACTGGCAATACCTG
AGTCAGCGGCTCCCTTGGGGATATCTGCGGGTAGACGAAAACAACGTGGTGCTGGAGTGCAATCCTACCGCCCAGCGCCT
GCTGCGCATCAGCCAATGGCAGCCGGGCATCAAGCTCTTGCTGGAATGGGTGCGCTCCTATGAGTTGGATCAGCTCATCG
AGGCTACCCGCCGACAGGAGACCGCTGCTCCTGTCCAAAGTCGCGAATGGATTTTCTACCCTTCCGGTGGGGATGGCAAA
CCGATTCCGCTGCGGGCTTGGGGGTTGCTCCTGCCGCAAGCGCACGTGGCCATTTTTTTAGAAGACCGGCTGGAAGCAAA
AATGCTGGCCCAACAGCGGGATCGCTGGGCTTCGGATGTGGCTCACGAGCTGAAAACCCCCCTCACCTCCATTCGCTTGC
TGGCCGAAACTTTACAAAGCCGGGTCGATGCCGCTCAAGCAGTCTGGGTGGAACGATTGCTAAATGAAACTCTGCGCCTG
AGCCATTTGGTTCAAGACTTGCTCGAACTCAGCGCCCTCAATTTGGGAGCCGCATCCCGCTCTCAGTGGCGCTGGGTGGA
TCTGGCAAGTCTGGTGCAGGAAGCCTGGCAAAGTGTAGAGCCGTTGGCCAAGCCTCGACACCAGGAACTGCACCTGAGCG
GATTGTCCCAGATTTACCTCTGGGGAGATGAGCAACGCCTATACCGCCTCATCCTCAATCTCTTGGACAACGCCGTCAAG
TATGGCAAGCCCGACTCTCCTATCCATGTGGTGCTTTCGGAGCAGGGATCCCAGGTGCAGTTAGAGGTGTACGACCACGG
CCCCGGCCTGCCAGAAGATCAATTTGAGTTGGTCTTTCAGCCCTTTTACCGCACCGACACTGCGCGGGCCCGCAGCAAAG
GCGGAACCGGCCTAGGGCTGGCAATTGTGCGACAAATTGTCGAAGTTCACGGGGGCACCATCCAACTGCGCAATCACCCG
CAAACCGGTGGGCTGTGGGTTCAGGTGGAACTGCCCCGGAAGCACCTGGATCCCTTGTGA

Upstream 100 bases:

>100_bases
CAGTACCACCCTCGTACATCGTCGATAAGAGGCCGGCAACCGGGCTTTCGCCTCCACTCTTGCTTCCCTGAGCGACAATG
GCAGCAAAGGTGGTGGGAAC

Downstream 100 bases:

>100_bases
AACCCGCCCGAAGACGCTCGCGTCAGTGGCCCGCAGCCAGAATCTGGACGAGAATCGCCTAAGATTTCCTTTCTTCAGGG
CTGTCCATCTCAGGGGTGAT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MGLEAGILIGLGIGLLCTLLLLWQSRRRFSRELQRLGSLVHLAMPLHSLEQMLSLLSRYLRQQDLDWANQKQQLQNWQYL
SQRLPWGYLRVDENNVVLECNPTAQRLLRISQWQPGIKLLLEWVRSYELDQLIEATRRQETAAPVQSREWIFYPSGGDGK
PIPLRAWGLLLPQAHVAIFLEDRLEAKMLAQQRDRWASDVAHELKTPLTSIRLLAETLQSRVDAAQAVWVERLLNETLRL
SHLVQDLLELSALNLGAASRSQWRWVDLASLVQEAWQSVEPLAKPRHQELHLSGLSQIYLWGDEQRLYRLILNLLDNAVK
YGKPDSPIHVVLSEQGSQVQLEVYDHGPGLPEDQFELVFQPFYRTDTARARSKGGTGLGLAIVRQIVEVHGGTIQLRNHP
QTGGLWVQVELPRKHLDPL

Sequences:

>Translated_419_residues
MGLEAGILIGLGIGLLCTLLLLWQSRRRFSRELQRLGSLVHLAMPLHSLEQMLSLLSRYLRQQDLDWANQKQQLQNWQYL
SQRLPWGYLRVDENNVVLECNPTAQRLLRISQWQPGIKLLLEWVRSYELDQLIEATRRQETAAPVQSREWIFYPSGGDGK
PIPLRAWGLLLPQAHVAIFLEDRLEAKMLAQQRDRWASDVAHELKTPLTSIRLLAETLQSRVDAAQAVWVERLLNETLRL
SHLVQDLLELSALNLGAASRSQWRWVDLASLVQEAWQSVEPLAKPRHQELHLSGLSQIYLWGDEQRLYRLILNLLDNAVK
YGKPDSPIHVVLSEQGSQVQLEVYDHGPGLPEDQFELVFQPFYRTDTARARSKGGTGLGLAIVRQIVEVHGGTIQLRNHP
QTGGLWVQVELPRKHLDPL
>Mature_418_residues
GLEAGILIGLGIGLLCTLLLLWQSRRRFSRELQRLGSLVHLAMPLHSLEQMLSLLSRYLRQQDLDWANQKQQLQNWQYLS
QRLPWGYLRVDENNVVLECNPTAQRLLRISQWQPGIKLLLEWVRSYELDQLIEATRRQETAAPVQSREWIFYPSGGDGKP
IPLRAWGLLLPQAHVAIFLEDRLEAKMLAQQRDRWASDVAHELKTPLTSIRLLAETLQSRVDAAQAVWVERLLNETLRLS
HLVQDLLELSALNLGAASRSQWRWVDLASLVQEAWQSVEPLAKPRHQELHLSGLSQIYLWGDEQRLYRLILNLLDNAVKY
GKPDSPIHVVLSEQGSQVQLEVYDHGPGLPEDQFELVFQPFYRTDTARARSKGGTGLGLAIVRQIVEVHGGTIQLRNHPQ
TGGLWVQVELPRKHLDPL

Specific function: Member of the two-component regulatory system sphR/sphS. Sensory kinase. Is involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to phos

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=222, Percent_Identity=36.036036036036, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790436, Length=246, Percent_Identity=32.9268292682927, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786912, Length=228, Percent_Identity=34.6491228070175, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790346, Length=236, Percent_Identity=31.7796610169492, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1786783, Length=245, Percent_Identity=29.3877551020408, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789808, Length=263, Percent_Identity=28.8973384030418, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789149, Length=237, Percent_Identity=28.6919831223629, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=339, Percent_Identity=24.7787610619469, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787894, Length=215, Percent_Identity=32.093023255814, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790551, Length=218, Percent_Identity=31.1926605504587, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790861, Length=267, Percent_Identity=27.7153558052434, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI145693157, Length=231, Percent_Identity=26.4069264069264, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI48994928, Length=233, Percent_Identity=23.6051502145923, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789403, Length=225, Percent_Identity=30.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=226, Percent_Identity=23.8938053097345, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=236, Percent_Identity=26.6949152542373, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787374, Length=241, Percent_Identity=25.3112033195021, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788279, Length=208, Percent_Identity=29.8076923076923, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48016; Mature: 47885

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLEAGILIGLGIGLLCTLLLLWQSRRRFSRELQRLGSLVHLAMPLHSLEQMLSLLSRYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQQDLDWANQKQQLQNWQYLSQRLPWGYLRVDENNVVLECNPTAQRLLRISQWQPGIKLL
HHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHHHCCCHHHHHH
LEWVRSYELDQLIEATRRQETAAPVQSREWIFYPSGGDGKPIPLRAWGLLLPQAHVAIFL
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCHHEEEEH
EDRLEAKMLAQQRDRWASDVAHELKTPLTSIRLLAETLQSRVDAAQAVWVERLLNETLRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHLVQDLLELSALNLGAASRSQWRWVDLASLVQEAWQSVEPLAKPRHQELHLSGLSQIYL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCCCHHHHHCCCCEEEE
WGDEQRLYRLILNLLDNAVKYGKPDSPIHVVLSEQGSQVQLEVYDHGPGLPEDQFELVFQ
ECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHH
PFYRTDTARARSKGGTGLGLAIVRQIVEVHGGTIQLRNHPQTGGLWVQVELPRKHLDPL
HHHHCHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCHHCCCCC
>Mature Secondary Structure 
GLEAGILIGLGIGLLCTLLLLWQSRRRFSRELQRLGSLVHLAMPLHSLEQMLSLLSRYL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQQDLDWANQKQQLQNWQYLSQRLPWGYLRVDENNVVLECNPTAQRLLRISQWQPGIKLL
HHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHHHCCCHHHHHH
LEWVRSYELDQLIEATRRQETAAPVQSREWIFYPSGGDGKPIPLRAWGLLLPQAHVAIFL
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCHHEEEEH
EDRLEAKMLAQQRDRWASDVAHELKTPLTSIRLLAETLQSRVDAAQAVWVERLLNETLRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHLVQDLLELSALNLGAASRSQWRWVDLASLVQEAWQSVEPLAKPRHQELHLSGLSQIYL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCCCHHHHHCCCCEEEE
WGDEQRLYRLILNLLDNAVKYGKPDSPIHVVLSEQGSQVQLEVYDHGPGLPEDQFELVFQ
ECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHH
PFYRTDTARARSKGGTGLGLAIVRQIVEVHGGTIQLRNHPQTGGLWVQVELPRKHLDPL
HHHHCHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497200 [H]