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The map label for this gene is phrB-2 [H]

Identifier: 86608285

GI number: 86608285

Start: 828856

End: 830316

Strand: Reverse

Name: phrB-2 [H]

Synonym: CYB_0803

Alternate gene names: 86608285

Gene position: 830316-828856 (Counterclockwise)

Preceding gene: 86608286

Following gene: 86608284

Centisome position: 27.25

GC content: 60.99

Gene sequence:

>1461_bases
ATGACTGTACTGCTGTGGTTTCGCACCGACTTGCGCCTGCTGGATCACCAGCCGCTGACGCGGGCCTGCCAACAGGGATC
CCCTTTGATCCCCTTGTATTGCTTGGATCCCCGCCAGTTCGGCGAGACTTCCCTTGGGCTCCGCCCCAGAACCAGCCCTT
TCCGCGGTCAGTTTTTGCTGGAGAGTTTGGCAGATCTGCGGCAGCAGTTGCGGTCGCGCGGCTCGGATTTGGTGATCCGC
CAGGGACAGCCAGAACAGGTGATCCCCACCTTGGCACAGGAGTGGGGGGTCGAGGCGGTCTATGCCCATGAAGAGGTGGG
CACCGAGGAGAAGGAGGTGGCGGCTGCTGTTGAGCGGGCTTTGCGGTCTCTGGGGATCCGGCTGCAGGTCGATTGGGGCC
ATACCCTCTATCACCCTGAGGATCTCCCCTTTTGTCTCTCGCAACTGCCGGAGCTGTTCACCCGCTTTCGAAAGCAAGTC
GAGGGAAGCACCCCCATCCGGGATCCCTTGCCCATTCCCTCGATCCCCCCCCTGCCATCGGGTTTGGATCCCGGCCCTCT
GCCCACCCTGGCCTCGCTGGGGCTATCTTCACCTGCTAGGGATCCACGGGCCCGCTTCACCTACACCGGCGGCAGCACTG
CTGCCCATGCCCGCCTGCAAACCTACTTTTGGGATCTGGATCGGCTGCGCATCTACAAAGAAACCCGCAACGGCATGTTG
GATCCCGACGATAGCTCCCGGCTTTCTGCTTGGTTGGCCCTGGGCTGCATCTCCCCTCGCACCGTCTATGCCGAGGTCAA
GCGTTACGAGCAGGAGCGGGTGCGCAATGAGTCCACCTACTGGCTGGTGTTTGAGCTGCTGTGGCGGGATTATTTTCGCT
TTATCTTGGCCAAGCACGGCGCCAAACTGTTCCGTCCTTCTGGGATCCAAGGGATCCCCATCCCTTGGCAGCAGGATTGG
CCGCGCTTTCAGGCTTGGTGTCGGGGTGAGACGGGCTACCCCTTGGTGGATGCCAACATGCGGGAGCTGGCGGCTACCGG
ATTTATGTCCAACCGGGGACGGCAGAATGTGGCCAGCTTTTTGACCAAGAACCTGGGCATCGACTGGCGCATGGGCGCTG
AGTGGTTTGAGTCGTTGCTCATCGACTACGACGTGGCCAGCAACTACGGCAATTGGAACTACACCGCCGGCGTAGGGAAT
GATGCCCGTGGCTTCCGCTACTTCCACATCCCCAAACAATCTCGCGAGTACGATCCCAAAGGAGACTATCTGCGCCACTG
GTTGCCGGAATTGGCCGGGATCCCGGGGGATAAAATCCACGAGCCCTATCGCCTCACTGCTGCCGAGCAGAAGCAATTTG
GAGTCCGATTGGGGGTGGACTACCCTCGCCCACTGGTGGATTTGGAGAAGTCTCTCGCGGCCAACCGACGGGTTTACGAA
GCTGCCCTGGCCTGCCGATAG

Upstream 100 bases:

>100_bases
CGATGTGCCTCATTTTGTTCCCAGCCCTGAGCTGGGGCGTCCAGCTGGGGTTCGGGTGTCCAAGTCTGAGTCCGCGGGAT
CCGTGTAGGGTAGCTGCAGA

Downstream 100 bases:

>100_bases
GCATTGCCTTGTCTGGGGCTTCTTCCCGAGAGGGGGCTGAGGGGATTTTCTGAGGCGCAGTTCCGGACCAAGAGTGCTAT
TCTAGTTAAGCACTATCCTG

Product: deoxyribodipyrimidine photolyase

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTVLLWFRTDLRLLDHQPLTRACQQGSPLIPLYCLDPRQFGETSLGLRPRTSPFRGQFLLESLADLRQQLRSRGSDLVIR
QGQPEQVIPTLAQEWGVEAVYAHEEVGTEEKEVAAAVERALRSLGIRLQVDWGHTLYHPEDLPFCLSQLPELFTRFRKQV
EGSTPIRDPLPIPSIPPLPSGLDPGPLPTLASLGLSSPARDPRARFTYTGGSTAAHARLQTYFWDLDRLRIYKETRNGML
DPDDSSRLSAWLALGCISPRTVYAEVKRYEQERVRNESTYWLVFELLWRDYFRFILAKHGAKLFRPSGIQGIPIPWQQDW
PRFQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIDWRMGAEWFESLLIDYDVASNYGNWNYTAGVGN
DARGFRYFHIPKQSREYDPKGDYLRHWLPELAGIPGDKIHEPYRLTAAEQKQFGVRLGVDYPRPLVDLEKSLAANRRVYE
AALACR

Sequences:

>Translated_486_residues
MTVLLWFRTDLRLLDHQPLTRACQQGSPLIPLYCLDPRQFGETSLGLRPRTSPFRGQFLLESLADLRQQLRSRGSDLVIR
QGQPEQVIPTLAQEWGVEAVYAHEEVGTEEKEVAAAVERALRSLGIRLQVDWGHTLYHPEDLPFCLSQLPELFTRFRKQV
EGSTPIRDPLPIPSIPPLPSGLDPGPLPTLASLGLSSPARDPRARFTYTGGSTAAHARLQTYFWDLDRLRIYKETRNGML
DPDDSSRLSAWLALGCISPRTVYAEVKRYEQERVRNESTYWLVFELLWRDYFRFILAKHGAKLFRPSGIQGIPIPWQQDW
PRFQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIDWRMGAEWFESLLIDYDVASNYGNWNYTAGVGN
DARGFRYFHIPKQSREYDPKGDYLRHWLPELAGIPGDKIHEPYRLTAAEQKQFGVRLGVDYPRPLVDLEKSLAANRRVYE
AALACR
>Mature_485_residues
TVLLWFRTDLRLLDHQPLTRACQQGSPLIPLYCLDPRQFGETSLGLRPRTSPFRGQFLLESLADLRQQLRSRGSDLVIRQ
GQPEQVIPTLAQEWGVEAVYAHEEVGTEEKEVAAAVERALRSLGIRLQVDWGHTLYHPEDLPFCLSQLPELFTRFRKQVE
GSTPIRDPLPIPSIPPLPSGLDPGPLPTLASLGLSSPARDPRARFTYTGGSTAAHARLQTYFWDLDRLRIYKETRNGMLD
PDDSSRLSAWLALGCISPRTVYAEVKRYEQERVRNESTYWLVFELLWRDYFRFILAKHGAKLFRPSGIQGIPIPWQQDWP
RFQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIDWRMGAEWFESLLIDYDVASNYGNWNYTAGVGND
ARGFRYFHIPKQSREYDPKGDYLRHWLPELAGIPGDKIHEPYRLTAAEQKQFGVRLGVDYPRPLVDLEKSLAANRRVYEA
ALACR

Specific function: May have a photoreceptor function. Binds DNA; probably functions as a transcriptional repressor [H]

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=477, Percent_Identity=30.188679245283, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI4758072, Length=484, Percent_Identity=29.3388429752066, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI188536103, Length=425, Percent_Identity=30.1176470588235, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786926, Length=489, Percent_Identity=31.0838445807771, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324962, Length=253, Percent_Identity=34.3873517786561, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137248, Length=508, Percent_Identity=28.1496062992126, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24585455, Length=508, Percent_Identity=28.1496062992126, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24648152, Length=522, Percent_Identity=26.4367816091954, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR014133
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 55649; Mature: 55518

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLLWFRTDLRLLDHQPLTRACQQGSPLIPLYCLDPRQFGETSLGLRPRTSPFRGQFLL
CEEEEEECCCHHHHCCCHHHHHHHCCCCEEEEEECCHHHHCCCCCCCCCCCCCCHHHHHH
ESLADLRQQLRSRGSDLVIRQGQPEQVIPTLAQEWGVEAVYAHEEVGTEEKEVAAAVERA
HHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCEEEEEHHHHCCHHHHHHHHHHHH
LRSLGIRLQVDWGHTLYHPEDLPFCLSQLPELFTRFRKQVEGSTPIRDPLPIPSIPPLPS
HHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GLDPGPLPTLASLGLSSPARDPRARFTYTGGSTAAHARLQTYFWDLDRLRIYKETRNGML
CCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DPDDSSRLSAWLALGCISPRTVYAEVKRYEQERVRNESTYWLVFELLWRDYFRFILAKHG
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
AKLFRPSGIQGIPIPWQQDWPRFQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASF
CHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHH
LTKNLGIDWRMGAEWFESLLIDYDVASNYGNWNYTAGVGNDARGFRYFHIPKQSREYDPK
HHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCC
GDYLRHWLPELAGIPGDKIHEPYRLTAAEQKQFGVRLGVDYPRPLVDLEKSLAANRRVYE
HHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHH
AALACR
HHHHCC
>Mature Secondary Structure 
TVLLWFRTDLRLLDHQPLTRACQQGSPLIPLYCLDPRQFGETSLGLRPRTSPFRGQFLL
EEEEEECCCHHHHCCCHHHHHHHCCCCEEEEEECCHHHHCCCCCCCCCCCCCCHHHHHH
ESLADLRQQLRSRGSDLVIRQGQPEQVIPTLAQEWGVEAVYAHEEVGTEEKEVAAAVERA
HHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCEEEEEHHHHCCHHHHHHHHHHHH
LRSLGIRLQVDWGHTLYHPEDLPFCLSQLPELFTRFRKQVEGSTPIRDPLPIPSIPPLPS
HHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GLDPGPLPTLASLGLSSPARDPRARFTYTGGSTAAHARLQTYFWDLDRLRIYKETRNGML
CCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DPDDSSRLSAWLALGCISPRTVYAEVKRYEQERVRNESTYWLVFELLWRDYFRFILAKHG
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
AKLFRPSGIQGIPIPWQQDWPRFQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASF
CHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHH
LTKNLGIDWRMGAEWFESLLIDYDVASNYGNWNYTAGVGNDARGFRYFHIPKQSREYDPK
HHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCC
GDYLRHWLPELAGIPGDKIHEPYRLTAAEQKQFGVRLGVDYPRPLVDLEKSLAANRRVYE
HHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHH
AALACR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]