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The map label for this gene is ispF

Identifier: 86608267

GI number: 86608267

Start: 807056

End: 807529

Strand: Direct

Name: ispF

Synonym: CYB_0783

Alternate gene names: 86608267

Gene position: 807056-807529 (Clockwise)

Preceding gene: 86608266

Following gene: 86608268

Centisome position: 26.49

GC content: 63.92

Gene sequence:

>474_bases
GTGCGCATTGGCCAGGGCTATGACATCCACCGCCTCGTCGAGGGGCGCCCCCTGATCCTAGGTGGGATCCGGATCCCTTA
CGAAAAAGGGCTGTTGGGCCACAGCGACGCCGACGTGCTCACCCACGCCATCATGGACGCTCTCCTGGGGGCCGCCGCCC
TGCGGGACATCGGCTACTACTTTCCCCCCGAAGACGAGCGCTGGAAGGGGGCAGACAGCATTCTCCTCTTGCAGCAGGTG
GTGCAACTGGTAGGCCAAGAGGGCTGGCGCATTGCCAATGTCGATAGCGTCGTGGTAGCGGAGCAGCCTAAGCTCAAGCC
CCACATCCCCGCCATACAGGCCCGCCTGGCCGAGGCCATGCAACTATCGCCCAAACAAGTAGGCGTGAAGGCCACCACCA
ACGAAAAACTGGGCCCCGTCGGCCAGGGAGAAGCCATGGCCGCCTTTGCCGTCGTCCTGTTGCTGGAACGCTGA

Upstream 100 bases:

>100_bases
GAGAGGTCGTTCTCAAAAGCCGCTTGTCCTCTCCCCGTGAGCCAAGCTCTTGGGAGGAAGTGGCAGAGGTCTCGATGGTT
TTCCCAAGGGAGCCGGAACA

Downstream 100 bases:

>100_bases
GCCGCCGCTTTCTGGGAAGCACCTGGTCATCGCGCTGCCCAAGCTGCCAAACTGGCAAGCAACCGCTTCAAAAGTTTGCC
CCAATTGCCGTGCAACCCCC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIGQGYDIHRLVEGRPLILGGIRIPYEKGLLGHSDADVLTHAIMDALLGAAALRDIGYYFPPEDERWKGADSILLLQQV
VQLVGQEGWRIANVDSVVVAEQPKLKPHIPAIQARLAEAMQLSPKQVGVKATTNEKLGPVGQGEAMAAFAVVLLLER

Sequences:

>Translated_157_residues
MRIGQGYDIHRLVEGRPLILGGIRIPYEKGLLGHSDADVLTHAIMDALLGAAALRDIGYYFPPEDERWKGADSILLLQQV
VQLVGQEGWRIANVDSVVVAEQPKLKPHIPAIQARLAEAMQLSPKQVGVKATTNEKLGPVGQGEAMAAFAVVLLLER
>Mature_157_residues
MRIGQGYDIHRLVEGRPLILGGIRIPYEKGLLGHSDADVLTHAIMDALLGAAALRDIGYYFPPEDERWKGADSILLLQQV
VQLVGQEGWRIANVDSVVVAEQPKLKPHIPAIQARLAEAMQLSPKQVGVKATTNEKLGPVGQGEAMAAFAVVLLLER

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=51.2820512820513, Blast_Score=149, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_SYNJA (Q2JXJ4)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_473751.1
- ProteinModelPortal:   Q2JXJ4
- SMR:   Q2JXJ4
- STRING:   Q2JXJ4
- GeneID:   3898090
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_0267
- TIGR:   CYA_0267
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   SSP321327:CYA_0267-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17049; Mature: 17049

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGQGYDIHRLVEGRPLILGGIRIPYEKGLLGHSDADVLTHAIMDALLGAAALRDIGYY
CCCCCCCCHHHHHCCCCEEEECEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
FPPEDERWKGADSILLLQQVVQLVGQEGWRIANVDSVVVAEQPKLKPHIPAIQARLAEAM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCHHHHHHHHHHH
QLSPKQVGVKATTNEKLGPVGQGEAMAAFAVVLLLER
HCCHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGQGYDIHRLVEGRPLILGGIRIPYEKGLLGHSDADVLTHAIMDALLGAAALRDIGYY
CCCCCCCCHHHHHCCCCEEEECEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
FPPEDERWKGADSILLLQQVVQLVGQEGWRIANVDSVVVAEQPKLKPHIPAIQARLAEAM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCHHHHHHHHHHH
QLSPKQVGVKATTNEKLGPVGQGEAMAAFAVVLLLER
HCCHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA