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The map label for this gene is purA [H]

Identifier: 86607581

GI number: 86607581

Start: 91835

End: 93184

Strand: Reverse

Name: purA [H]

Synonym: CYB_0079

Alternate gene names: 86607581

Gene position: 93184-91835 (Counterclockwise)

Preceding gene: 86607582

Following gene: 86607579

Centisome position: 3.06

GC content: 59.41

Gene sequence:

>1350_bases
TTGGCAAACGTCGTGGTTGTCGGTGCCCAGTGGGGCGATGAAGGAAAAGGGAAGATCACCGACCTCCTCAGTGAAAAGGC
CCATGTGGTCGTCCGCTATCAAGGTGGGGTGAATGCAGGGCATACCCTGGTGGTGGGGGGCCAAACCTTCAAGCTACACT
TGATCCCCTCTGGCATTCTCTACCCCGACAAACGCTGCATCATCGCCAGCGGCACGGTGATCGATCCCGAGGTTCTGCTG
GCCGAGATCGACCAACTGCATCAATTGGGCATTTCCACCGACAACCTGTTCATTGCCGAAACGGCCCACGTTACCCTGCC
CTACCACCGGGTCATTGACATCGCAGAAGAGGAGCGGCGTGGCATTTACCGGCTGGGCACCACCGGTCGGGGCATTGGCC
CCACCTATGCCGACAAGGCCGAGCGCATGGGCATTCGCGTCGTGGACTTGATGTACCCTGACCAGCTGCGGGAGCGCCTC
AGTTGGGCCATTCCCTACAAAAATGTCTTGCTGGAAAAGATCTATAACTTGCCGCCCTTGGATCCCGAGCCAATTATCGA
GCAATACCTGGCCTATGCCGAGCGGCTGCGCCCCTATGTTACCGACGCTGCTCTGCTTCTCACCCAAGCCATCGAAGATC
GGGAGAACATCCTGTTTGAAGGGGCTCAAGGCACCCTGTTGGATCTCGACTACGGCACCTACCCCTACGTCACCTCCTCC
CATCCCATTGCCGGTGGCGCTTGTATTGGCGCTGGGATCGGCCCCACCAGCATTGACCGGGTGATCGGGGTGGCCAAAGC
CTACACCACCCGCGTTGGCGAAGGCCCTTTCCCCACCGAGCTCAAAGATGAGATCGGGGCGTACCTGGGGGCAACCGGTG
CTGAATTTGGCACCACCACAGGGCGACAACGGCGCTGCGGCTGGTTTGACGGGGTAATTGGGCGCTACGCGGTGCGAATC
AATGGCCTAGACTGCCTGGCCATCACCAAACTGGATGTGCTGGATGGCTTGGATGAGATCAAGGTGTGCGTGGCCTACGA
GTACCAGGGGCGGGAAATTCGTCACTTTCCCAGCGATGCCCGGGTTTTCGCCCAGTGCAAACCCATCTACGAAACCCTGC
CGGGCTGGAAATGTTCCACCAAAGATTGCCGCTCCATCCAAGACTTGCCCCCCGAAGCCCATGATTACCTGAAGTTTTTG
GCACAGTTGATGCAGGTGCCGATTGCCATTGTTTCTCTGGGGGCCAGCCGCGACCAGACGATCATTGTCGAAGATCCCAT
CCACGGCCCCAAGCGGGCCCTTCTCTACCCCAATGGTGGCAAGCGCGCCCACGCCCTCATGCCCGACTGA

Upstream 100 bases:

>100_bases
TCCTGATCTCTCAACATCGCCCGGAGCGATGCGGTAGATTAGTGCAGCAACCCCCGCAAAGGGGATTTTTTGTGCCATCT
ACGGACTGTAGGAGAAGCTC

Downstream 100 bases:

>100_bases
GTGGGCTCAAGAACAATTGGGAACAAGGGCAATCATAGGGCAATCATTAACAATGTAGTGATCAAATGCTCAGTAATGAT
CAATGTGAGTTAAGTTATGG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MANVVVVGAQWGDEGKGKITDLLSEKAHVVVRYQGGVNAGHTLVVGGQTFKLHLIPSGILYPDKRCIIASGTVIDPEVLL
AEIDQLHQLGISTDNLFIAETAHVTLPYHRVIDIAEEERRGIYRLGTTGRGIGPTYADKAERMGIRVVDLMYPDQLRERL
SWAIPYKNVLLEKIYNLPPLDPEPIIEQYLAYAERLRPYVTDAALLLTQAIEDRENILFEGAQGTLLDLDYGTYPYVTSS
HPIAGGACIGAGIGPTSIDRVIGVAKAYTTRVGEGPFPTELKDEIGAYLGATGAEFGTTTGRQRRCGWFDGVIGRYAVRI
NGLDCLAITKLDVLDGLDEIKVCVAYEYQGREIRHFPSDARVFAQCKPIYETLPGWKCSTKDCRSIQDLPPEAHDYLKFL
AQLMQVPIAIVSLGASRDQTIIVEDPIHGPKRALLYPNGGKRAHALMPD

Sequences:

>Translated_449_residues
MANVVVVGAQWGDEGKGKITDLLSEKAHVVVRYQGGVNAGHTLVVGGQTFKLHLIPSGILYPDKRCIIASGTVIDPEVLL
AEIDQLHQLGISTDNLFIAETAHVTLPYHRVIDIAEEERRGIYRLGTTGRGIGPTYADKAERMGIRVVDLMYPDQLRERL
SWAIPYKNVLLEKIYNLPPLDPEPIIEQYLAYAERLRPYVTDAALLLTQAIEDRENILFEGAQGTLLDLDYGTYPYVTSS
HPIAGGACIGAGIGPTSIDRVIGVAKAYTTRVGEGPFPTELKDEIGAYLGATGAEFGTTTGRQRRCGWFDGVIGRYAVRI
NGLDCLAITKLDVLDGLDEIKVCVAYEYQGREIRHFPSDARVFAQCKPIYETLPGWKCSTKDCRSIQDLPPEAHDYLKFL
AQLMQVPIAIVSLGASRDQTIIVEDPIHGPKRALLYPNGGKRAHALMPD
>Mature_448_residues
ANVVVVGAQWGDEGKGKITDLLSEKAHVVVRYQGGVNAGHTLVVGGQTFKLHLIPSGILYPDKRCIIASGTVIDPEVLLA
EIDQLHQLGISTDNLFIAETAHVTLPYHRVIDIAEEERRGIYRLGTTGRGIGPTYADKAERMGIRVVDLMYPDQLRERLS
WAIPYKNVLLEKIYNLPPLDPEPIIEQYLAYAERLRPYVTDAALLLTQAIEDRENILFEGAQGTLLDLDYGTYPYVTSSH
PIAGGACIGAGIGPTSIDRVIGVAKAYTTRVGEGPFPTELKDEIGAYLGATGAEFGTTTGRQRRCGWFDGVIGRYAVRIN
GLDCLAITKLDVLDGLDEIKVCVAYEYQGREIRHFPSDARVFAQCKPIYETLPGWKCSTKDCRSIQDLPPEAHDYLKFLA
QLMQVPIAIVSLGASRDQTIIVEDPIHGPKRALLYPNGGKRAHALMPD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=43.7939110070258, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=43.559718969555, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=42.3173803526448, Blast_Score=309, Evalue=4e-84,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=49.6487119437939, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25146553, Length=431, Percent_Identity=40.1392111368909, Blast_Score=307, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI25146555, Length=431, Percent_Identity=40.1392111368909, Blast_Score=306, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=43.0232558139535, Blast_Score=342, Evalue=7e-95,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=42.2169811320755, Blast_Score=341, Evalue=7e-94,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 49352; Mature: 49221

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGAQWGDEGKGKITDLLSEKAHVVVRYQGGVNAGHTLVVGGQTFKLHLIPSGIL
CCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCCEEEEEEECCCCC
YPDKRCIIASGTVIDPEVLLAEIDQLHQLGISTDNLFIAETAHVTLPYHRVIDIAEEERR
CCCCEEEEECCCEECHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECHHHHHHHHHHHCC
GIYRLGTTGRGIGPTYADKAERMGIRVVDLMYPDQLRERLSWAIPYKNVLLEKIYNLPPL
CEEEECCCCCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCCC
DPEPIIEQYLAYAERLRPYVTDAALLLTQAIEDRENILFEGAQGTLLDLDYGTYPYVTSS
CHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCEECC
HPIAGGACIGAGIGPTSIDRVIGVAKAYTTRVGEGPFPTELKDEIGAYLGATGAEFGTTT
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
GRQRRCGWFDGVIGRYAVRINGLDCLAITKLDVLDGLDEIKVCVAYEYQGREIRHFPSDA
CCCCCCCHHHHHHEEEEEEECCCEEEEEEHHHHHCCCCCEEEEEEEEECCCEEECCCCCH
RVFAQCKPIYETLPGWKCSTKDCRSIQDLPPEAHDYLKFLAQLMQVPIAIVSLGASRDQT
HHHHHCCHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCE
IIVEDPIHGPKRALLYPNGGKRAHALMPD
EEEECCCCCCCEEEEECCCCCEECCCCCC
>Mature Secondary Structure 
ANVVVVGAQWGDEGKGKITDLLSEKAHVVVRYQGGVNAGHTLVVGGQTFKLHLIPSGIL
CCEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCCEEEEEEECCCCC
YPDKRCIIASGTVIDPEVLLAEIDQLHQLGISTDNLFIAETAHVTLPYHRVIDIAEEERR
CCCCEEEEECCCEECHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECHHHHHHHHHHHCC
GIYRLGTTGRGIGPTYADKAERMGIRVVDLMYPDQLRERLSWAIPYKNVLLEKIYNLPPL
CEEEECCCCCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCCC
DPEPIIEQYLAYAERLRPYVTDAALLLTQAIEDRENILFEGAQGTLLDLDYGTYPYVTSS
CHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCEECC
HPIAGGACIGAGIGPTSIDRVIGVAKAYTTRVGEGPFPTELKDEIGAYLGATGAEFGTTT
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
GRQRRCGWFDGVIGRYAVRINGLDCLAITKLDVLDGLDEIKVCVAYEYQGREIRHFPSDA
CCCCCCCHHHHHHEEEEEEECCCEEEEEEHHHHHCCCCCEEEEEEEEECCCEEECCCCCH
RVFAQCKPIYETLPGWKCSTKDCRSIQDLPPEAHDYLKFLAQLMQVPIAIVSLGASRDQT
HHHHHCCHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCE
IIVEDPIHGPKRALLYPNGGKRAHALMPD
EEEECCCCCCCEEEEECCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA