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The map label for this gene is mocBf

Identifier: 86361030

GI number: 86361030

Start: 337543

End: 338472

Strand: Reverse

Name: mocBf

Synonym: RHE_PF00300

Alternate gene names: 86361030

Gene position: 338472-337543 (Counterclockwise)

Preceding gene: 86361031

Following gene: 86361029

Centisome position: 52.68

GC content: 60.75

Gene sequence:

>930_bases
ATGAAAACCGCCACGCTTGCATCCATCGCCTTGGCGATGTCGGTCACTGTCGCGAATGCGCAGACGATCGGCGTCTCAAT
GTCCGATCTCGATAAATTCAGAACGGCGCTGCTGAACGGCGTCGTCAACCACGGGCAGACAATATCAGGCCTCAAGCTCG
TCACCGAAAACGCGAAGGGCGACAACGAGCTGCAGAAGAAGCAGGTTCAGAAGCTGATTGCCGACAAGGTCGATGCCATC
ATCCTGGCCGTTTCCGATGGCGATCTCGGGCCGCAGATGACCAAGATGGCGGCGGATGCCGGCATTCCGCTGGTTTACAT
CAATAACGTCCCATCCAATCTTCTGGATCTTCCCGAAAACCAGGTGGTGGTCGCCTCCAACGAGAAGGAATCCGGCACGC
TGGAGACAAAGCAGGTCTGCGCTCTGCTCACGGGCAAGGGCCGTGTCGCCGTGCTGATGGGCGAACCCTTCCACGCGGCC
GCCCGCGCCCGCACCCAGGACATCTCCGATGTCATCGCCACGCCCGAGTGTAAGGGCCTTGAAATCGTCGAGCGGCAGGC
GGCCTATTGGTCGCGCGATTATGCCGACCTGCAGATGCAGGAATGGCTTTCGGCCGGCGTCAAATTCGATGCCGTTATCG
CCAACAATGACGAGATGGCGCTTGGCGCGATCCGCGCCATGAAGAAGGCCGGCATCCCGATGAAGGACGTCGTCGTCGCC
GGCGTCGACGCGACTGATGATGCGCTCGCTGCGATGACGGCCGGCGATCTTGATGTCACCATTCTCCAGAGCGCCGTCGG
GCAGGGGGCGGCGGCAGTCGACGCGGCCGTCAAGCTCGTCAACAAGGAGAAGGTGCCGCGCGAAAACAACGTTCCCTTCG
AACTCGTCACCCCTCAGAACATTGCCCAATACCTGCCGAAGAGCCAGTGA

Upstream 100 bases:

>100_bases
GCTTTCCATCCCCTGCGTTAGTTCTCGCTTTCGCACAAAAAGTAAAATTTAACCAAGATTTGAAATAACGCCATTCTATC
CGAATTGCGCAGGGGAATTC

Downstream 100 bases:

>100_bases
GCATAAGAGGCCTATGATGTTGCATTTCTGGAATAAATTCGGCATTCGCGCGCAGATCACCGCAGGCTTCGTGCCGCTGA
TCCTGCTGATGAGCCTGCTG

Product: rhizopine ABC transporter, substrate-binding protein

Products: ADP; phosphate; beta-D-galactose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKTATLASIALAMSVTVANAQTIGVSMSDLDKFRTALLNGVVNHGQTISGLKLVTENAKGDNELQKKQVQKLIADKVDAI
ILAVSDGDLGPQMTKMAADAGIPLVYINNVPSNLLDLPENQVVVASNEKESGTLETKQVCALLTGKGRVAVLMGEPFHAA
ARARTQDISDVIATPECKGLEIVERQAAYWSRDYADLQMQEWLSAGVKFDAVIANNDEMALGAIRAMKKAGIPMKDVVVA
GVDATDDALAAMTAGDLDVTILQSAVGQGAAAVDAAVKLVNKEKVPRENNVPFELVTPQNIAQYLPKSQ

Sequences:

>Translated_309_residues
MKTATLASIALAMSVTVANAQTIGVSMSDLDKFRTALLNGVVNHGQTISGLKLVTENAKGDNELQKKQVQKLIADKVDAI
ILAVSDGDLGPQMTKMAADAGIPLVYINNVPSNLLDLPENQVVVASNEKESGTLETKQVCALLTGKGRVAVLMGEPFHAA
ARARTQDISDVIATPECKGLEIVERQAAYWSRDYADLQMQEWLSAGVKFDAVIANNDEMALGAIRAMKKAGIPMKDVVVA
GVDATDDALAAMTAGDLDVTILQSAVGQGAAAVDAAVKLVNKEKVPRENNVPFELVTPQNIAQYLPKSQ
>Mature_309_residues
MKTATLASIALAMSVTVANAQTIGVSMSDLDKFRTALLNGVVNHGQTISGLKLVTENAKGDNELQKKQVQKLIADKVDAI
ILAVSDGDLGPQMTKMAADAGIPLVYINNVPSNLLDLPENQVVVASNEKESGTLETKQVCALLTGKGRVAVLMGEPFHAA
ARARTQDISDVIATPECKGLEIVERQAAYWSRDYADLQMQEWLSAGVKFDAVIANNDEMALGAIRAMKKAGIPMKDVVVA
GVDATDDALAAMTAGDLDVTILQSAVGQGAAAVDAAVKLVNKEKVPRENNVPFELVTPQNIAQYLPKSQ

Specific function: Involved in rhizopine (L-3-O-methyl-scyllo-inosamine) catabolism. Could be involved in its high affinity transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788473, Length=333, Percent_Identity=28.2282282282282, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790192, Length=305, Percent_Identity=30.8196721311475, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 4720 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32679; Mature: 32679

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTATLASIALAMSVTVANAQTIGVSMSDLDKFRTALLNGVVNHGQTISGLKLVTENAKG
CCCHHHHHHHHHHHEEEECCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
DNELQKKQVQKLIADKVDAIILAVSDGDLGPQMTKMAADAGIPLVYINNVPSNLLDLPEN
CHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCCC
QVVVASNEKESGTLETKQVCALLTGKGRVAVLMGEPFHAAARARTQDISDVIATPECKGL
CEEEECCCCCCCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCH
EIVERQAAYWSRDYADLQMQEWLSAGVKFDAVIANNDEMALGAIRAMKKAGIPMKDVVVA
HHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHEEEE
GVDATDDALAAMTAGDLDVTILQSAVGQGAAAVDAAVKLVNKEKVPRENNVPFELVTPQN
CCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECHHH
IAQYLPKSQ
HHHHCCCCC
>Mature Secondary Structure
MKTATLASIALAMSVTVANAQTIGVSMSDLDKFRTALLNGVVNHGQTISGLKLVTENAKG
CCCHHHHHHHHHHHEEEECCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
DNELQKKQVQKLIADKVDAIILAVSDGDLGPQMTKMAADAGIPLVYINNVPSNLLDLPEN
CHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCCC
QVVVASNEKESGTLETKQVCALLTGKGRVAVLMGEPFHAAARARTQDISDVIATPECKGL
CEEEECCCCCCCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCH
EIVERQAAYWSRDYADLQMQEWLSAGVKFDAVIANNDEMALGAIRAMKKAGIPMKDVVVA
HHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHEEEE
GVDATDDALAAMTAGDLDVTILQSAVGQGAAAVDAAVKLVNKEKVPRENNVPFELVTPQN
CCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECHHH
IAQYLPKSQ
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; beta-D-galactose [Periplasm]; H2O [C]

Specific reaction: ATP + beta-D-galactose [Periplasm] + H2O = ADP + phosphate + beta-D-galactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7845353 [H]