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The map label for this gene is fliY [H]

Identifier: 86360893

GI number: 86360893

Start: 170818

End: 171603

Strand: Reverse

Name: fliY [H]

Synonym: RHE_PF00162

Alternate gene names: 86360893

Gene position: 171603-170818 (Counterclockwise)

Preceding gene: 86360894

Following gene: 86360892

Centisome position: 26.71

GC content: 61.58

Gene sequence:

>786_bases
ATGCTGACAAGACGGATCTTCTTCGCAATCGCGACCCTGGCCGCGACCTTCGGCTTCAACGCGCCGTCTCACGCCGATGC
TCTCGCCGATATTACGGCGCGCGGTACGCTGCGTGTCGCAGTCCCGCAGGATTTCCCGCCCTTCGGCAGTGTCGGCACCG
ATATGGCGCCCATGGGCTACGATATCGACATGGCCAACCTCATTGCCGAAAAGCTCGGCGTCAAAGCCGAGCTGGTGCCG
GTCACGAGCGCCAACCGCGTCCCCTATCTGCAGACGAACAAGGTCGATCTCGTGATATCGAGCCTCGGCAAGAATCCGGA
GCGCGAGAAGGTGATCGATTTCTCGGTCGCCTACGCGCCCTTCTTCAACGGCGTGTTCGGGCCGGCCGATCTTTCGGTCG
CCAAGGCGGAAGATCTCGCGGGCAAGAGCATCGGAGTCACGCGCGGCGCCGTGGAGGATCTCGAGCTGACGAAGATCGCG
CCGGCCGATGCCACGATAAAGCGTTACGAGGACAATAATGGCACCATCTCCGCCTTCCTGTCCGGCCAGGTCGACATCAT
TGCCACCGGCAACGTCGTGGCGGCTGCGATCCTCGCCAAAAATCCTCCCAAGCGCCCGGAGCTCAAGTTCCTGATCAAGA
ACTCGCCCTGCTATATCGGCCTCAACAAGGAACAGTCCGCGCTTCTGGAGAAGGTGAACGGCATTGTCGCCGCCGCCAAA
GCCGATGGCACGCTGAACGCCATATCGCAGAAGTGGCTCGGTGCCGATCTGCCGTCCGATCTGTAA

Upstream 100 bases:

>100_bases
TCGGATCTGCGCAATTCCCTGTCGCTTCCGCTAAAGGCCGCGTCCGCCATCTCCCGGCAATCAGACCCCAGAACCACAAC
TTCAAAGGAGCAATAAACAC

Downstream 100 bases:

>100_bases
GGAAACCGGGGTCTCGCGCATGTCTCCTGCGGCCGCGGGATGACATGCGCTCCGTCACCGCAAGAGGGGACATTCCTTGA
GCTACCATTTCGAATTCAGT

Product: amino acid ABC transporter substrate-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLTRRIFFAIATLAATFGFNAPSHADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGYDIDMANLIAEKLGVKAELVP
VTSANRVPYLQTNKVDLVISSLGKNPEREKVIDFSVAYAPFFNGVFGPADLSVAKAEDLAGKSIGVTRGAVEDLELTKIA
PADATIKRYEDNNGTISAFLSGQVDIIATGNVVAAAILAKNPPKRPELKFLIKNSPCYIGLNKEQSALLEKVNGIVAAAK
ADGTLNAISQKWLGADLPSDL

Sequences:

>Translated_261_residues
MLTRRIFFAIATLAATFGFNAPSHADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGYDIDMANLIAEKLGVKAELVP
VTSANRVPYLQTNKVDLVISSLGKNPEREKVIDFSVAYAPFFNGVFGPADLSVAKAEDLAGKSIGVTRGAVEDLELTKIA
PADATIKRYEDNNGTISAFLSGQVDIIATGNVVAAAILAKNPPKRPELKFLIKNSPCYIGLNKEQSALLEKVNGIVAAAK
ADGTLNAISQKWLGADLPSDL
>Mature_261_residues
MLTRRIFFAIATLAATFGFNAPSHADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGYDIDMANLIAEKLGVKAELVP
VTSANRVPYLQTNKVDLVISSLGKNPEREKVIDFSVAYAPFFNGVFGPADLSVAKAEDLAGKSIGVTRGAVEDLELTKIA
PADATIKRYEDNNGTISAFLSGQVDIIATGNVVAAAILAKNPPKRPELKFLIKNSPCYIGLNKEQSALLEKVNGIVAAAK
ADGTLNAISQKWLGADLPSDL

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=28.1368821292776, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=27.6, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788648, Length=238, Percent_Identity=25.6302521008403, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=23.7154150197628, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787031, Length=265, Percent_Identity=27.1698113207547, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788649, Length=228, Percent_Identity=22.8070175438596, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27573; Mature: 27573

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRIFFAIATLAATFGFNAPSHADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGY
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCC
DIDMANLIAEKLGVKAELVPVTSANRVPYLQTNKVDLVISSLGKNPEREKVIDFSVAYAP
CCHHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCEEEEEHHCCCCCHHHEEEEEEEHHH
FFNGVFGPADLSVAKAEDLAGKSIGVTRGAVEDLELTKIAPADATIKRYEDNNGTISAFL
HHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEE
SGQVDIIATGNVVAAAILAKNPPKRPELKFLIKNSPCYIGLNKEQSALLEKVNGIVAAAK
CCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHCCEEEEEC
ADGTLNAISQKWLGADLPSDL
CCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLTRRIFFAIATLAATFGFNAPSHADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGY
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCC
DIDMANLIAEKLGVKAELVPVTSANRVPYLQTNKVDLVISSLGKNPEREKVIDFSVAYAP
CCHHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCEEEEEHHCCCCCHHHEEEEEEEHHH
FFNGVFGPADLSVAKAEDLAGKSIGVTRGAVEDLELTKIAPADATIKRYEDNNGTISAFL
HHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEE
SGQVDIIATGNVVAAAILAKNPPKRPELKFLIKNSPCYIGLNKEQSALLEKVNGIVAAAK
CCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHCCEEEEEC
ADGTLNAISQKWLGADLPSDL
CCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]