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The map label for this gene is fcl [C]

Identifier: 85860195

GI number: 85860195

Start: 2396271

End: 2397239

Strand: Direct

Name: fcl [C]

Synonym: SYN_00585

Alternate gene names: 85860195

Gene position: 2396271-2397239 (Clockwise)

Preceding gene: 85860194

Following gene: 85860196

Centisome position: 75.37

GC content: 53.46

Gene sequence:

>969_bases
ATGCCGACAGACTTTACGGATAAGCGCATCGCGGTGACGGGCGGCAAGGGATTTCTGGGAAAGCATCTGATTTCCCGCCT
GCAGGAACGAGGCTGTCGTCAAATCATCATCGTGGACCTCCCCGAATATCATCTGGTTCATCAGGATGATATCCGGAAGA
TGTACGGTGAGGTAAAGCCGGATATCGTGATTCATCTCGCTGCCAAGGTCGGGGGAATCGGGTTCAATCAGGCCAATCCG
GCAACCCTTTTCTATGAAAATCTGATGATGGGCGTCCAACTGCTTCATGAAGGCTGGGCCCAGGGAATTGAAAAGTTTGT
CGGCATCGGAACCATCTGCGCGTATCCCAAATTCACCCCCGTTCCCTTTAAAGAAGAGGATATCTGGAACGGCTACCCGG
AAGAAACCAATGCCCCCTACGGACTCGCCAAGAAAATGATGCTGGTCCAGGCTCAGGCTTATCGGCAGCAATATGGCTTC
AACGCCATTTTCCTGCTTCCGGTCAACCTTTACGGACCCGGAGACAACTTCGACCCCCAGTCCTCGCACGTCATTCCGGC
CCTGATCAAAAAATGTGTTGACGCCCGGCTGCAAAAAGAAGAGGAAATCATGGTCTGGGGAACGGGACAGGCCACACGGG
AATTCTTCTATGTCGAAGACGCGGCGGAAGCCATCGTTCTGGCCGCGGAAAAGTACGATAAAAGTGAACCGGTCAATATC
GGCGCCGGTTTTGAAATATCCATCCGTGACCTGGTGACGCTCATCGCGGAATTGACCGGATTCACGGGTCGGATCGTCTG
GGACAGCTCCCGTCCGGATGGTCAGCCCCGACGGATGCTGGACACCCGTCGGGCTTATGAAGAATTCGGATTTCAGGCTA
AAACCGACTTTCGCCTGGGGTTGAAAAAAACCATCGACTGGTATGTTGAACAGAGGAGCCGGGAACTCGGCACAAAGTTG
ATTCTGTAG

Upstream 100 bases:

>100_bases
GCCGCGGACCTGGAACTCGCTCAGCGGGAGAAAACGCTGACGGACGCCGGTTACTCCTGCAATCTGAACCCGCATCTCTG
CATTTAATAAAGGAGGATTC

Downstream 100 bases:

>100_bases
GAAAAATATGCAGGAATAAAATTTAAACCTATATCATTTATTCTCTGCTTTCTGGTTGACTTCAGGAATGGAATCATGGT
AGGTAGCACACCGCAAAGAG

Product: GDP-L-fucose synthase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPTDFTDKRIAVTGGKGFLGKHLISRLQERGCRQIIIVDLPEYHLVHQDDIRKMYGEVKPDIVIHLAAKVGGIGFNQANP
ATLFYENLMMGVQLLHEGWAQGIEKFVGIGTICAYPKFTPVPFKEEDIWNGYPEETNAPYGLAKKMMLVQAQAYRQQYGF
NAIFLLPVNLYGPGDNFDPQSSHVIPALIKKCVDARLQKEEEIMVWGTGQATREFFYVEDAAEAIVLAAEKYDKSEPVNI
GAGFEISIRDLVTLIAELTGFTGRIVWDSSRPDGQPRRMLDTRRAYEEFGFQAKTDFRLGLKKTIDWYVEQRSRELGTKL
IL

Sequences:

>Translated_322_residues
MPTDFTDKRIAVTGGKGFLGKHLISRLQERGCRQIIIVDLPEYHLVHQDDIRKMYGEVKPDIVIHLAAKVGGIGFNQANP
ATLFYENLMMGVQLLHEGWAQGIEKFVGIGTICAYPKFTPVPFKEEDIWNGYPEETNAPYGLAKKMMLVQAQAYRQQYGF
NAIFLLPVNLYGPGDNFDPQSSHVIPALIKKCVDARLQKEEEIMVWGTGQATREFFYVEDAAEAIVLAAEKYDKSEPVNI
GAGFEISIRDLVTLIAELTGFTGRIVWDSSRPDGQPRRMLDTRRAYEEFGFQAKTDFRLGLKKTIDWYVEQRSRELGTKL
IL
>Mature_321_residues
PTDFTDKRIAVTGGKGFLGKHLISRLQERGCRQIIIVDLPEYHLVHQDDIRKMYGEVKPDIVIHLAAKVGGIGFNQANPA
TLFYENLMMGVQLLHEGWAQGIEKFVGIGTICAYPKFTPVPFKEEDIWNGYPEETNAPYGLAKKMMLVQAQAYRQQYGFN
AIFLLPVNLYGPGDNFDPQSSHVIPALIKKCVDARLQKEEEIMVWGTGQATREFFYVEDAAEAIVLAAEKYDKSEPVNIG
AGFEISIRDLVTLIAELTGFTGRIVWDSSRPDGQPRRMLDTRRAYEEFGFQAKTDFRLGLKKTIDWYVEQRSRELGTKLI
L

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=312, Percent_Identity=32.3717948717949, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI7657641, Length=350, Percent_Identity=26.2857142857143, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI42516563, Length=324, Percent_Identity=26.2345679012346, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1788365, Length=319, Percent_Identity=33.8557993730408, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=23.7142857142857, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=24.4956772334294, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554428, Length=309, Percent_Identity=30.0970873786408, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17539532, Length=325, Percent_Identity=27.3846153846154, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=337, Percent_Identity=26.7062314540059, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=333, Percent_Identity=24.6246246246246, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19922778, Length=314, Percent_Identity=30.2547770700637, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI21356223, Length=327, Percent_Identity=26.9113149847095, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 36472; Mature: 36341

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDFTDKRIAVTGGKGFLGKHLISRLQERGCRQIIIVDLPEYHLVHQDDIRKMYGEVKP
CCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCC
DIVIHLAAKVGGIGFNQANPATLFYENLMMGVQLLHEGWAQGIEKFVGIGTICAYPKFTP
CEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC
VPFKEEDIWNGYPEETNAPYGLAKKMMLVQAQAYRQQYGFNAIFLLPVNLYGPGDNFDPQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCC
SSHVIPALIKKCVDARLQKEEEIMVWGTGQATREFFYVEDAAEAIVLAAEKYDKSEPVNI
CCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHEEEEEECHHHEEEEEHHHCCCCCCEEE
GAGFEISIRDLVTLIAELTGFTGRIVWDSSRPDGQPRRMLDTRRAYEEFGFQAKTDFRLG
CCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
LKKTIDWYVEQRSRELGTKLIL
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTDFTDKRIAVTGGKGFLGKHLISRLQERGCRQIIIVDLPEYHLVHQDDIRKMYGEVKP
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCC
DIVIHLAAKVGGIGFNQANPATLFYENLMMGVQLLHEGWAQGIEKFVGIGTICAYPKFTP
CEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC
VPFKEEDIWNGYPEETNAPYGLAKKMMLVQAQAYRQQYGFNAIFLLPVNLYGPGDNFDPQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCC
SSHVIPALIKKCVDARLQKEEEIMVWGTGQATREFFYVEDAAEAIVLAAEKYDKSEPVNI
CCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHEEEEEECHHHEEEEEHHHCCCCCCEEE
GAGFEISIRDLVTLIAELTGFTGRIVWDSSRPDGQPRRMLDTRRAYEEFGFQAKTDFRLG
CCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
LKKTIDWYVEQRSRELGTKLIL
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]