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The map label for this gene is gmd [H]

Identifier: 85860194

GI number: 85860194

Start: 2395223

End: 2396257

Strand: Direct

Name: gmd [H]

Synonym: SYN_00584

Alternate gene names: 85860194

Gene position: 2395223-2396257 (Clockwise)

Preceding gene: 85860193

Following gene: 85860195

Centisome position: 75.34

GC content: 56.33

Gene sequence:

>1035_bases
ATGAAAAAAGCATTTATCACCGGCATTACAGGACAGGACGGCTCCTATCTGGCTGAATTTCTCCTCGGGAAAGGCTATGA
AGTCCACGGCCTGATCCGGCGCTCCAGTACCTTCAATACGGACCGCATCGACCATCTGTACAAGGATTTCCACGATCCTC
AGGCCAGGCTTTATCTGCATTACGGAGATCTCTCCGTTTCCGGTCAGTTGACTGACATGCTCTACGACATCAGTCCCGAT
GAAATCTATCACCTCGGCGCCCAGAGCCACGTGCGGGTCAGCTTCGACATGCCCGAATACACGGGAGACGTCACCGGCCT
CGGAACCCTCAGGCTCCTGGAGGCGATCCGGAAAACCGGCGTAAAGGCCCGTTTTTATCAGGCTTCCTCAAGTGAAATGT
TCGGGGCCGCCCCCCCTCCACAGCATGAAGGGACCCTTTTCCAGCCGCAAAGCCCTTACGCCGCCGCCAAGGTCTATGCC
TATTACGTCGTCAGGAATTACCGGGACGCTTACAAGCTGTTTGCCTGCAATGGAATCCTGTTCAACCATGAATCCCCCCG
GCGGGGTGAAACCTTCGTCACCCGGAAGATCACCCGGGGTGCTACCCAGATCAAGCTTGGTCTTCGGGACAAGCTCTATC
TGGGCAACCTGGAAGCAAAAAGAGACTGGGGCTTTGCCGGCGATTATGTGGAAGCCATGTGGCTGATGCTCCAGCAGGAC
AAGCCGGACGACTACGTAATCGCCACGGGGGAAACCCATTCCGTCCGGGAGTTTGCCGAGAAGGTCTTTCAGAAGCTGGA
TCTCGACTACCATCAGTATGTGGAAATCGATCCCCGCTACTTCCGCCCCACGGAGGTGGACGTTCTGCTCGGCGACTCAT
CCAAAGCCAGACGCCTCCTGAACTGGAAGCCGCGTGTCAGCTTCGATGAACTGGTCGAAATGATGATCGCCGCGGACCTG
GAACTCGCTCAGCGGGAGAAAACGCTGACGGACGCCGGTTACTCCTGCAATCTGAACCCGCATCTCTGCATTTAA

Upstream 100 bases:

>100_bases
CTTTCGAAGCGGCCTTTGTTTCCTTTGCCCATTCCGATGAAGATTTAGAGAAAACGCTGCAGGCCTGCGCTGAAACCATA
AAAACTTTGGGAGGTAACCC

Downstream 100 bases:

>100_bases
TAAAGGAGGATTCATGCCGACAGACTTTACGGATAAGCGCATCGCGGTGACGGGCGGCAAGGGATTTCTGGGAAAGCATC
TGATTTCCCGCCTGCAGGAA

Product: GDP-mannose 4,6 dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKKAFITGITGQDGSYLAEFLLGKGYEVHGLIRRSSTFNTDRIDHLYKDFHDPQARLYLHYGDLSVSGQLTDMLYDISPD
EIYHLGAQSHVRVSFDMPEYTGDVTGLGTLRLLEAIRKTGVKARFYQASSSEMFGAAPPPQHEGTLFQPQSPYAAAKVYA
YYVVRNYRDAYKLFACNGILFNHESPRRGETFVTRKITRGATQIKLGLRDKLYLGNLEAKRDWGFAGDYVEAMWLMLQQD
KPDDYVIATGETHSVREFAEKVFQKLDLDYHQYVEIDPRYFRPTEVDVLLGDSSKARRLLNWKPRVSFDELVEMMIAADL
ELAQREKTLTDAGYSCNLNPHLCI

Sequences:

>Translated_344_residues
MKKAFITGITGQDGSYLAEFLLGKGYEVHGLIRRSSTFNTDRIDHLYKDFHDPQARLYLHYGDLSVSGQLTDMLYDISPD
EIYHLGAQSHVRVSFDMPEYTGDVTGLGTLRLLEAIRKTGVKARFYQASSSEMFGAAPPPQHEGTLFQPQSPYAAAKVYA
YYVVRNYRDAYKLFACNGILFNHESPRRGETFVTRKITRGATQIKLGLRDKLYLGNLEAKRDWGFAGDYVEAMWLMLQQD
KPDDYVIATGETHSVREFAEKVFQKLDLDYHQYVEIDPRYFRPTEVDVLLGDSSKARRLLNWKPRVSFDELVEMMIAADL
ELAQREKTLTDAGYSCNLNPHLCI
>Mature_344_residues
MKKAFITGITGQDGSYLAEFLLGKGYEVHGLIRRSSTFNTDRIDHLYKDFHDPQARLYLHYGDLSVSGQLTDMLYDISPD
EIYHLGAQSHVRVSFDMPEYTGDVTGLGTLRLLEAIRKTGVKARFYQASSSEMFGAAPPPQHEGTLFQPQSPYAAAKVYA
YYVVRNYRDAYKLFACNGILFNHESPRRGETFVTRKITRGATQIKLGLRDKLYLGNLEAKRDWGFAGDYVEAMWLMLQQD
KPDDYVIATGETHSVREFAEKVFQKLDLDYHQYVEIDPRYFRPTEVDVLLGDSSKARRLLNWKPRVSFDELVEMMIAADL
ELAQREKTLTDAGYSCNLNPHLCI

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=58.8921282798834, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1788366, Length=367, Percent_Identity=53.133514986376, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=22.8412256267409, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1786974, Length=339, Percent_Identity=25.6637168141593, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=56.1046511627907, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI133901790, Length=344, Percent_Identity=54.6511627906977, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17539424, Length=344, Percent_Identity=54.6511627906977, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17539422, Length=344, Percent_Identity=54.6511627906977, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI133901786, Length=344, Percent_Identity=54.6511627906977, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI133901788, Length=344, Percent_Identity=54.6511627906977, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24158427, Length=348, Percent_Identity=57.4712643678161, Blast_Score=401, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39347; Mature: 39347

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAFITGITGQDGSYLAEFLLGKGYEVHGLIRRSSTFNTDRIDHLYKDFHDPQARLYLH
CCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
YGDLSVSGQLTDMLYDISPDEIYHLGAQSHVRVSFDMPEYTGDVTGLGTLRLLEAIRKTG
ECCEEECCCHHHHEEECCHHHEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCC
VKARFYQASSSEMFGAAPPPQHEGTLFQPQSPYAAAKVYAYYVVRNYRDAYKLFACNGIL
CEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECEEE
FNHESPRRGETFVTRKITRGATQIKLGLRDKLYLGNLEAKRDWGFAGDYVEAMWLMLQQD
ECCCCCCCCCHHEEHHHHCCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
KPDDYVIATGETHSVREFAEKVFQKLDLDYHQYVEIDPRYFRPTEVDVLLGDSSKARRLL
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHEEEECCCEECCCEEEEEECCCHHHHHHH
NWKPRVSFDELVEMMIAADLELAQREKTLTDAGYSCNLNPHLCI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKAFITGITGQDGSYLAEFLLGKGYEVHGLIRRSSTFNTDRIDHLYKDFHDPQARLYLH
CCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
YGDLSVSGQLTDMLYDISPDEIYHLGAQSHVRVSFDMPEYTGDVTGLGTLRLLEAIRKTG
ECCEEECCCHHHHEEECCHHHEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCC
VKARFYQASSSEMFGAAPPPQHEGTLFQPQSPYAAAKVYAYYVVRNYRDAYKLFACNGIL
CEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECEEE
FNHESPRRGETFVTRKITRGATQIKLGLRDKLYLGNLEAKRDWGFAGDYVEAMWLMLQQD
ECCCCCCCCCHHEEHHHHCCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
KPDDYVIATGETHSVREFAEKVFQKLDLDYHQYVEIDPRYFRPTEVDVLLGDSSKARRLL
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHEEEECCCEECCCEEEEEECCCHHHHHHH
NWKPRVSFDELVEMMIAADLELAQREKTLTDAGYSCNLNPHLCI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]