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The map label for this gene is cspLA [H]

Identifier: 85859293

GI number: 85859293

Start: 1477390

End: 1477590

Strand: Direct

Name: cspLA [H]

Synonym: SYN_02175

Alternate gene names: 85859293

Gene position: 1477390-1477590 (Clockwise)

Preceding gene: 85859292

Following gene: 85859294

Centisome position: 46.47

GC content: 48.76

Gene sequence:

>201_bases
TTGGCACAGGGGAGAGTTAAATGGTTCAACGATAAAAAGGGTTTTGGCTTTATCGAAAATGATGAAGGCGGTGACGTATT
CGTCCATTTCAGTTCCATCCAGGGAGCAGGCTGGAAGACGCTTAACGAAAGCCAGAGGGTTCGCTTTGAAATTCAGCAGG
GCCAGAAAGGGCCCAGCGCGGTTAATGTCGAACTGATCTGA

Upstream 100 bases:

>100_bases
AACCACGGTCGGCTCTTGATTAGGATCATGAAGCCGGGGTGGTTTTTTTTGTTTCTGCTTCAATAGGCATCAAGAAATAA
AAGAAAAGGAGGGTACGACT

Downstream 100 bases:

>100_bases
GCTGTCGTAAGAAAAAGAGTACGTGGATCGGCGTAACCTGGTAATTTATTTGTATTGATAACGGAATTAAAAAAGCTTGA
CATGTAATCCCAAAAGGGAT

Product: cold shock protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 66; Mature: 65

Protein sequence:

>66_residues
MAQGRVKWFNDKKGFGFIENDEGGDVFVHFSSIQGAGWKTLNESQRVRFEIQQGQKGPSAVNVELI

Sequences:

>Translated_66_residues
MAQGRVKWFNDKKGFGFIENDEGGDVFVHFSSIQGAGWKTLNESQRVRFEIQQGQKGPSAVNVELI
>Mature_65_residues
AQGRVKWFNDKKGFGFIENDEGGDVFVHFSSIQGAGWKTLNESQRVRFEIQQGQKGPSAVNVELI

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=67.6923076923077, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=66.1290322580645, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI2367114, Length=61, Percent_Identity=62.2950819672131, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=59.375, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787834, Length=61, Percent_Identity=60.655737704918, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=58.0645161290323, Blast_Score=72, Evalue=5e-15,
Organism=Escherichia coli, GI1787107, Length=61, Percent_Identity=52.4590163934426, Blast_Score=67, Evalue=2e-13,

Paralogues:

None

Copy number: 8300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7414; Mature: 7283

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQGRVKWFNDKKGFGFIENDEGGDVFVHFSSIQGAGWKTLNESQRVRFEIQQGQKGPSA
CCCCCEEEECCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCE
VNVELI
EEEEEC
>Mature Secondary Structure 
AQGRVKWFNDKKGFGFIENDEGGDVFVHFSSIQGAGWKTLNESQRVRFEIQQGQKGPSA
CCCCEEEECCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCE
VNVELI
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]