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The map label for this gene is cls [H]

Identifier: 85858350

GI number: 85858350

Start: 514701

End: 516179

Strand: Direct

Name: cls [H]

Synonym: SYN_02342

Alternate gene names: 85858350

Gene position: 514701-516179 (Clockwise)

Preceding gene: 85858349

Following gene: 85858351

Centisome position: 16.19

GC content: 54.16

Gene sequence:

>1479_bases
ATGAGAGGCACTATACAAGTCGACAACCGTGAGCAGGATATCCTGGCAGAGATGCGCGTCCTCGCTAACCAAGCCTTCTC
ACGCGCTGCCGGTGCTCCTCTCGTCGAAGGAAATCATGTTCGACTGCTCAAAGACGCGAGTGAGAATTATCCGGCGTGGC
TTAATGCCATCGGCGGCGCGAAACATCACATCCACATAGAGAACTATATCATTCGTGAAGACGATACAGGACATTTGTTC
GCTGACGCTCTGATTGCAAAAGCCAAGGAAGGCGTACGCGTGCGGCTAATCTACGATTGGATGGGTGGATTCGGCAAAAC
TTCTCAGCGGTTCTGGGACCGCTTATATCTGGGAGGGGTCGAGGTTCGTTGTTACAATCCGCCGCGTCTTGACAGCCCGC
TCGGTTGGCTCTCACGCGATCACCGCAAGATGCTCGCAATTGATGGAAAAATAGGCTTCATAACAGGCTTGTGCATCGGG
CAGATGTGGGAAGGCATCCCTGAAAAGGGTATCGAGCCGTGGCGTGACACCGGAGTCGAAGTGCGTGGGATTGCCGTCGC
AGACGTCGAGCAAGCATTTGCGCAAGTCTGGGCTTTGATGGGTGAGCCGATCCCTGAACATGAAATAGTCAAAAGAGAAG
CGCTTTGTCGGGCCGGCGATATGAGTATGCGCGTTGTGGCGAGTATGCCGACGACGGCCGGCATGTTCCGCTTAGACCAA
CTCATCGTCGCGCTCGCCAGAAAGAGGCTGTGGTTAACGGATGCTTACTACGCGGGGACGAGCGCCTACGTGCAGGCGCT
GAGAGCGGCATCGAAGGACGGAGTTGACGTGCGCCTGCTTGTGCCGAACGGAACAGACATCCCTATTTTGAGACTTTTAG
CCAGGGCAGGTTATCGCCCGCTCCTGGAGGCGGGAGTTAGGATCTTTGAGTGGAACGGCACGATGCTGCATGCGAAAACG
GCAATAGCCGACGAAGGCTGGGCACGTGTCGGATCGACGAATCTGAACGTTGCCAGTTGGTTCGGCAATTGCGAATTGGA
CGCTGTGGTCGAAGACGAATCTTTCGTGCAGGAGATGGAAGAGATGTACCTTCAGGATTTAACGAATGCGACAGAAATCG
TCCTGGACGCAAAACTGAAGGTGTGCGCGCCGGGTGAGCCGCACTTGACGCACAAGGCAATGAAAAGAGGCGGTGGGAGT
GTCGGAAGAGCAGCAGCCGGAACGATTCGTGTGAGTAACGTTATCGGCGCAGCATTCACCGATCGGCGCGTCTTGGAGCC
CTTTGAGGCGCGCTTGATGACAGTTGTTGGAACTTGCTTACTGGTATTAGCCAGCCTCTTTGCATACTTCCCGCGTGGGC
TTGCGTACCCAGTGTTAATTATTTTCGTTTGGTTTGGTGTCGCTCTGCTTTACAGAAGCTATAAGTTGCATCTCAAAGGC
AAGCAAACAAAAGCATCGGATACAAGCAGAAAACTGTGA

Upstream 100 bases:

>100_bases
GAGCAGGGACAGAAAGCGATAGCAAGGCAGAGACAAAAATTCACGGAAACCTTCGGCCTATAGGGTTTTAAAGACCGATT
ACATTTTTACGGATGAGGCA

Downstream 100 bases:

>100_bases
TGAACTGTAAGAGGACGGACTTGAGTTGACAGTTCGTGAAAAAAGATGGTCAGATACCTCCCGGATTGTTAGGCAGAGTT
TACTTGACAGCATTTGTAAA

Product: cardiolipin synthetase

Products: CMP; 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MRGTIQVDNREQDILAEMRVLANQAFSRAAGAPLVEGNHVRLLKDASENYPAWLNAIGGAKHHIHIENYIIREDDTGHLF
ADALIAKAKEGVRVRLIYDWMGGFGKTSQRFWDRLYLGGVEVRCYNPPRLDSPLGWLSRDHRKMLAIDGKIGFITGLCIG
QMWEGIPEKGIEPWRDTGVEVRGIAVADVEQAFAQVWALMGEPIPEHEIVKREALCRAGDMSMRVVASMPTTAGMFRLDQ
LIVALARKRLWLTDAYYAGTSAYVQALRAASKDGVDVRLLVPNGTDIPILRLLARAGYRPLLEAGVRIFEWNGTMLHAKT
AIADEGWARVGSTNLNVASWFGNCELDAVVEDESFVQEMEEMYLQDLTNATEIVLDAKLKVCAPGEPHLTHKAMKRGGGS
VGRAAAGTIRVSNVIGAAFTDRRVLEPFEARLMTVVGTCLLVLASLFAYFPRGLAYPVLIIFVWFGVALLYRSYKLHLKG
KQTKASDTSRKL

Sequences:

>Translated_492_residues
MRGTIQVDNREQDILAEMRVLANQAFSRAAGAPLVEGNHVRLLKDASENYPAWLNAIGGAKHHIHIENYIIREDDTGHLF
ADALIAKAKEGVRVRLIYDWMGGFGKTSQRFWDRLYLGGVEVRCYNPPRLDSPLGWLSRDHRKMLAIDGKIGFITGLCIG
QMWEGIPEKGIEPWRDTGVEVRGIAVADVEQAFAQVWALMGEPIPEHEIVKREALCRAGDMSMRVVASMPTTAGMFRLDQ
LIVALARKRLWLTDAYYAGTSAYVQALRAASKDGVDVRLLVPNGTDIPILRLLARAGYRPLLEAGVRIFEWNGTMLHAKT
AIADEGWARVGSTNLNVASWFGNCELDAVVEDESFVQEMEEMYLQDLTNATEIVLDAKLKVCAPGEPHLTHKAMKRGGGS
VGRAAAGTIRVSNVIGAAFTDRRVLEPFEARLMTVVGTCLLVLASLFAYFPRGLAYPVLIIFVWFGVALLYRSYKLHLKG
KQTKASDTSRKL
>Mature_492_residues
MRGTIQVDNREQDILAEMRVLANQAFSRAAGAPLVEGNHVRLLKDASENYPAWLNAIGGAKHHIHIENYIIREDDTGHLF
ADALIAKAKEGVRVRLIYDWMGGFGKTSQRFWDRLYLGGVEVRCYNPPRLDSPLGWLSRDHRKMLAIDGKIGFITGLCIG
QMWEGIPEKGIEPWRDTGVEVRGIAVADVEQAFAQVWALMGEPIPEHEIVKREALCRAGDMSMRVVASMPTTAGMFRLDQ
LIVALARKRLWLTDAYYAGTSAYVQALRAASKDGVDVRLLVPNGTDIPILRLLARAGYRPLLEAGVRIFEWNGTMLHAKT
AIADEGWARVGSTNLNVASWFGNCELDAVVEDESFVQEMEEMYLQDLTNATEIVLDAKLKVCAPGEPHLTHKAMKRGGGS
VGRAAAGTIRVSNVIGAAFTDRRVLEPFEARLMTVVGTCLLVLASLFAYFPRGLAYPVLIIFVWFGVALLYRSYKLHLKG
KQTKASDTSRKL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=340, Percent_Identity=31.4705882352941, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787502, Length=373, Percent_Identity=24.9329758713137, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.5

Molecular weight: Translated: 54565; Mature: 54565

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGTIQVDNREQDILAEMRVLANQAFSRAAGAPLVEGNHVRLLKDASENYPAWLNAIGGA
CCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHCCCC
KHHIHIENYIIREDDTGHLFADALIAKAKEGVRVRLIYDWMGGFGKTSQRFWDRLYLGGV
CEEEEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCE
EVRCYNPPRLDSPLGWLSRDHRKMLAIDGKIGFITGLCIGQMWEGIPEKGIEPWRDTGVE
EEEECCCCCCCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCE
VRGIAVADVEQAFAQVWALMGEPIPEHEIVKREALCRAGDMSMRVVASMPTTAGMFRLDQ
EEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
LIVALARKRLWLTDAYYAGTSAYVQALRAASKDGVDVRLLVPNGTDIPILRLLARAGYRP
HHHHHHHHHHEEEHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCH
LLEAGVRIFEWNGTMLHAKTAIADEGWARVGSTNLNVASWFGNCELDAVVEDESFVQEME
HHHCCCEEEEECCEEEEEEHHHCCCCHHHCCCCCCEEEEECCCCEEEEEECCHHHHHHHH
EMYLQDLTNATEIVLDAKLKVCAPGEPHLTHKAMKRGGGSVGRAAAGTIRVSNVIGAAFT
HHHHHHHCCHHEEEEECCEEEECCCCCHHHHHHHHCCCCCCCCHHCCEEEEHHHHHHHHC
DRRVLEPFEARLMTVVGTCLLVLASLFAYFPRGLAYPVLIIFVWFGVALLYRSYKLHLKG
CCHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECC
KQTKASDTSRKL
CCCCCCCCCCCC
>Mature Secondary Structure
MRGTIQVDNREQDILAEMRVLANQAFSRAAGAPLVEGNHVRLLKDASENYPAWLNAIGGA
CCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHCCCC
KHHIHIENYIIREDDTGHLFADALIAKAKEGVRVRLIYDWMGGFGKTSQRFWDRLYLGGV
CEEEEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCE
EVRCYNPPRLDSPLGWLSRDHRKMLAIDGKIGFITGLCIGQMWEGIPEKGIEPWRDTGVE
EEEECCCCCCCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCE
VRGIAVADVEQAFAQVWALMGEPIPEHEIVKREALCRAGDMSMRVVASMPTTAGMFRLDQ
EEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
LIVALARKRLWLTDAYYAGTSAYVQALRAASKDGVDVRLLVPNGTDIPILRLLARAGYRP
HHHHHHHHHHEEEHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCH
LLEAGVRIFEWNGTMLHAKTAIADEGWARVGSTNLNVASWFGNCELDAVVEDESFVQEME
HHHCCCEEEEECCEEEEEEHHHCCCCHHHCCCCCCEEEEECCCCEEEEEECCHHHHHHHH
EMYLQDLTNATEIVLDAKLKVCAPGEPHLTHKAMKRGGGSVGRAAAGTIRVSNVIGAAFT
HHHHHHHCCHHEEEEECCEEEECCCCCHHHHHHHHCCCCCCCCHHCCEEEEHHHHHHHHC
DRRVLEPFEARLMTVVGTCLLVLASLFAYFPRGLAYPVLIIFVWFGVALLYRSYKLHLKG
CCHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECC
KQTKASDTSRKL
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CDPdiacylglycerol; sn-glycerol 3-phosphate

Specific reaction: CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9443601 [H]