Click here to switch to the map view.

The map label for this gene is usf [H]

Identifier: 85858346

GI number: 85858346

Start: 512477

End: 513358

Strand: Direct

Name: usf [H]

Synonym: SYN_02346

Alternate gene names: 85858346

Gene position: 512477-513358 (Clockwise)

Preceding gene: 85858345

Following gene: 85858347

Centisome position: 16.12

GC content: 54.65

Gene sequence:

>882_bases
TTGTCAAAATATCAATGTCTCCAATACACGAAAGAGAAGGTTTTTATTAAGAAAATCTCGAAGGAGGGCCATGCCATGAA
GCGCATCATGGTTATATCTCTTATTATCGCGGGCGTGCTTACGATCGCCAACACTGCCGTCGCTGCGCATAAGATCATTG
GCCGGAACGTTGAATACACGGCTGGCGGCATAACGATGAAAGGGTACCTGGCCTATGACGGAAAGGTCCAAGGAAAACAT
CCGGGTGTGCTCGTCGTCCATGAGTGGTGGGGACAGAACACCTACGTCCGCAAACGAGTCCGCATGCTGGCGCGGCTTGG
CTATACGGTCTTGGCGCTCGATATGTACGGCGAAGGCCGGACCGCACAGCATCCAGATGATGCCGGCAGGTTCGCTTCCG
AGGTGATGAAGAACTTTCCCGTAGCCAAGGAACGGTTCCTGGCAGCATTGGAGTTCCTGAAGAAGCAGCCGACCGTGGAC
CCTGACCGGATCGCAGCCATTGGCTACTGCTTCGGAGGAGGCGTCGTTCTGAACATGGCGCGCCAGGGCGTGGACCTGAA
GGGTGTTGCAAGCTTCCACGGCAGTCTGGCGGTAGTAAAGCCCGACCAGCCAACGCCTATCAAGGCAGCAGTGCGTGTTT
ATAACGGAGCTGACGACAAGTTTTCCACGCCCGAACAGATCGAGGCGATCAAGAAAGAGATGGCCGAGCAGAAGGTGAAC
TTCAAGTTCGTGAACTACCGCGGTGCAGTACACAGCTTCACGAACCCCGAAGCCACAGAGATCGGCAAGAAGTTCAAGAT
GCCCCTTGCCTATAACGCCAAGGCGGATAAGGAGTCCTGGGCGGACATGAGGCAGTTCCTGAAGGTGATATTCACAAAGT
AG

Upstream 100 bases:

>100_bases
TTGTAACATAGCATCCAGCAATTCTGACAGAGATCCATCTTTTCAATTGGTAATAGTTCATTCAAGATTTTCAATAATTA
TGATATAAAATAAAAAATAA

Downstream 100 bases:

>100_bases
CCGGTGAAAGAGTGAGCCTCTCCGCCAAAGCTGAATAAGGCTTATCTGATCCCCCTCTCATACCGGAAATAGCAATCATT
ATGGTACAAGGCCTGAAGCC

Product: dienelactone hydrolase family protein

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSKYQCLQYTKEKVFIKKISKEGHAMKRIMVISLIIAGVLTIANTAVAAHKIIGRNVEYTAGGITMKGYLAYDGKVQGKH
PGVLVVHEWWGQNTYVRKRVRMLARLGYTVLALDMYGEGRTAQHPDDAGRFASEVMKNFPVAKERFLAALEFLKKQPTVD
PDRIAAIGYCFGGGVVLNMARQGVDLKGVASFHGSLAVVKPDQPTPIKAAVRVYNGADDKFSTPEQIEAIKKEMAEQKVN
FKFVNYRGAVHSFTNPEATEIGKKFKMPLAYNAKADKESWADMRQFLKVIFTK

Sequences:

>Translated_293_residues
MSKYQCLQYTKEKVFIKKISKEGHAMKRIMVISLIIAGVLTIANTAVAAHKIIGRNVEYTAGGITMKGYLAYDGKVQGKH
PGVLVVHEWWGQNTYVRKRVRMLARLGYTVLALDMYGEGRTAQHPDDAGRFASEVMKNFPVAKERFLAALEFLKKQPTVD
PDRIAAIGYCFGGGVVLNMARQGVDLKGVASFHGSLAVVKPDQPTPIKAAVRVYNGADDKFSTPEQIEAIKKEMAEQKVN
FKFVNYRGAVHSFTNPEATEIGKKFKMPLAYNAKADKESWADMRQFLKVIFTK
>Mature_292_residues
SKYQCLQYTKEKVFIKKISKEGHAMKRIMVISLIIAGVLTIANTAVAAHKIIGRNVEYTAGGITMKGYLAYDGKVQGKHP
GVLVVHEWWGQNTYVRKRVRMLARLGYTVLALDMYGEGRTAQHPDDAGRFASEVMKNFPVAKERFLAALEFLKKQPTVDP
DRIAAIGYCFGGGVVLNMARQGVDLKGVASFHGSLAVVKPDQPTPIKAAVRVYNGADDKFSTPEQIEAIKKEMAEQKVNF
KFVNYRGAVHSFTNPEATEIGKKFKMPLAYNAKADKESWADMRQFLKVIFTK

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI17536419, Length=270, Percent_Identity=32.5925925925926, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17532655, Length=218, Percent_Identity=35.7798165137615, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17532653, Length=226, Percent_Identity=33.1858407079646, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193204305, Length=216, Percent_Identity=33.7962962962963, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17534829, Length=154, Percent_Identity=35.0649350649351, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17536415, Length=264, Percent_Identity=26.1363636363636, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 32669; Mature: 32537

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYQCLQYTKEKVFIKKISKEGHAMKRIMVISLIIAGVLTIANTAVAAHKIIGRNVEYT
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
AGGITMKGYLAYDGKVQGKHPGVLVVHEWWGQNTYVRKRVRMLARLGYTVLALDMYGEGR
CCCEEEEEEEEECCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCC
TAQHPDDAGRFASEVMKNFPVAKERFLAALEFLKKQPTVDPDRIAAIGYCFGGGVVLNMA
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHEEEHEECCCHHHHHHH
RQGVDLKGVASFHGSLAVVKPDQPTPIKAAVRVYNGADDKFSTPEQIEAIKKEMAEQKVN
HCCCCCEEHHHCCCCEEEECCCCCCCHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHCC
FKFVNYRGAVHSFTNPEATEIGKKFKMPLAYNAKADKESWADMRQFLKVIFTK
EEEEEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKYQCLQYTKEKVFIKKISKEGHAMKRIMVISLIIAGVLTIANTAVAAHKIIGRNVEYT
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
AGGITMKGYLAYDGKVQGKHPGVLVVHEWWGQNTYVRKRVRMLARLGYTVLALDMYGEGR
CCCEEEEEEEEECCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCC
TAQHPDDAGRFASEVMKNFPVAKERFLAALEFLKKQPTVDPDRIAAIGYCFGGGVVLNMA
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHEEEHEECCCHHHHHHH
RQGVDLKGVASFHGSLAVVKPDQPTPIKAAVRVYNGADDKFSTPEQIEAIKKEMAEQKVN
HCCCCCEEHHHCCCCEEEECCCCCCCHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHCC
FKFVNYRGAVHSFTNPEATEIGKKFKMPLAYNAKADKESWADMRQFLKVIFTK
EEEEEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]