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The map label for this gene is intB [H]

Identifier: 85375745

GI number: 85375745

Start: 2937805

End: 2939028

Strand: Direct

Name: intB [H]

Synonym: ELI_14590

Alternate gene names: 85375745

Gene position: 2937805-2939028 (Clockwise)

Preceding gene: 85375743

Following gene: 85375746

Centisome position: 96.25

GC content: 56.37

Gene sequence:

>1224_bases
ATGCCTCTCACCGAAACTCGGCTCCGAGCCCTGAAGAAGAAAACCAAATCGTACAAGGTCGCCGACCAGCGAGGCCTCTA
TATCGAGGTCACGCCGGCAGGAAGTAAGCTGTGGCGTTTCCGCTATCGTATCGGCAAAGTTGAGAAGAAGCTTGCCATCG
GCAGTTATCCGGAAATCAGTCTGAAGGAAGCGCGAGACGTGACCTTTGAGGCGCGCCAAGCTGTTGCCTCAGGTCGGGAC
CCTGCCTTTGAGAAACGCAAGCAGAAGATACGCGAGGAGTTTCTCTCCGCGCAGACCTTCGAGACGGTGGCCCGCGAATA
CATCGAGCAGATGATGGTTCAGAATGGCCGAGCTGAGGCCACGATCGTAAAGGCCAACTACTTTCTAGACCAGCTGGCAC
CGGCAATTGGAAACCGGCCCATTCATGAGATCGAGCCCTTTGAAGTCTTGGCTCCTCTCAAGCGGCTCGAGGCCACCGGG
AAACACGAAACCGCGAAAAAGTGTCGTTCGTTCGCGGGCAGGGTCTTCAGGTATGGCGTTGCGACAACTCGCTGCAAATC
CGATCCAACCAGCATGCTCAAAGGTGCACTGATAACGCCGAAGGCTACCCATTATGCGGCCATCCTGGAACCCAAGGAGC
TCGGCGGTCTGCTACGGGCAATCGACGACTACACCGGCTACATGGTGACAAAGTTCGCATTGCAGATCGCGCCTCATGTC
TTCGTACGACCTGGCGAACTCAGGCATGCCGAGTGGCGCGAGGTCGACCTTGTCGATGGTGTCTGGAAGATCCCCGCAGG
AAAAATGAAGGCTCGCCGTGCGCACGCCGTCCCTCTTTCGAAACAGGTCATAAGCTATCTGACAGACTTAGCCGAGATGC
TAGGCCATGAAGGCTATGTGTTTCCTTCGGCTCGGACTTCAAAGCGTCCTATGAGCGAGAACACCCTGAATGCCGCTTTT
CGCCGAATGGGATACTCCAAGGATGAGGTCACCGCCCACGGTCTGCGAGCTACGGCCTCGACATTCCTCAACGAGTCAGG
ACTTTGGAACCCGGATGCCATCGAGCGTGCATTGGCGCACGGCGACAGCAATGCGGTGCGTGGTATTTACCACCGTGGTA
AGCACTGGGATGAACGGGTGCGTATGGCTCAATGGTGGAGCGACTATCTTGATGAACTTCGCTCAGGAGGAAAGGTCATA
GAGGCGAAGTTTGCCCGAGGCTAA

Upstream 100 bases:

>100_bases
CGGTCGTTCCGGATCGTCCCGTTTGTTCTCGGGGGTTCCAGACATTTTTGCGGGCCTTTTGCGGGCCTTTGCGAAAACCA
CATTGGGAAAAGGCCCGCAC

Downstream 100 bases:

>100_bases
ATCGCGGCTGGGCCCCATTTGCAGATGGATCAAAGCAGTAAGGCTAGCGGTCGGGCTTCGTGCCTGGGAAATGACGTTGG
CGAGTTCTGAAGGATCGCAA

Product: symbiosis island integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MPLTETRLRALKKKTKSYKVADQRGLYIEVTPAGSKLWRFRYRIGKVEKKLAIGSYPEISLKEARDVTFEARQAVASGRD
PAFEKRKQKIREEFLSAQTFETVAREYIEQMMVQNGRAEATIVKANYFLDQLAPAIGNRPIHEIEPFEVLAPLKRLEATG
KHETAKKCRSFAGRVFRYGVATTRCKSDPTSMLKGALITPKATHYAAILEPKELGGLLRAIDDYTGYMVTKFALQIAPHV
FVRPGELRHAEWREVDLVDGVWKIPAGKMKARRAHAVPLSKQVISYLTDLAEMLGHEGYVFPSARTSKRPMSENTLNAAF
RRMGYSKDEVTAHGLRATASTFLNESGLWNPDAIERALAHGDSNAVRGIYHRGKHWDERVRMAQWWSDYLDELRSGGKVI
EAKFARG

Sequences:

>Translated_407_residues
MPLTETRLRALKKKTKSYKVADQRGLYIEVTPAGSKLWRFRYRIGKVEKKLAIGSYPEISLKEARDVTFEARQAVASGRD
PAFEKRKQKIREEFLSAQTFETVAREYIEQMMVQNGRAEATIVKANYFLDQLAPAIGNRPIHEIEPFEVLAPLKRLEATG
KHETAKKCRSFAGRVFRYGVATTRCKSDPTSMLKGALITPKATHYAAILEPKELGGLLRAIDDYTGYMVTKFALQIAPHV
FVRPGELRHAEWREVDLVDGVWKIPAGKMKARRAHAVPLSKQVISYLTDLAEMLGHEGYVFPSARTSKRPMSENTLNAAF
RRMGYSKDEVTAHGLRATASTFLNESGLWNPDAIERALAHGDSNAVRGIYHRGKHWDERVRMAQWWSDYLDELRSGGKVI
EAKFARG
>Mature_406_residues
PLTETRLRALKKKTKSYKVADQRGLYIEVTPAGSKLWRFRYRIGKVEKKLAIGSYPEISLKEARDVTFEARQAVASGRDP
AFEKRKQKIREEFLSAQTFETVAREYIEQMMVQNGRAEATIVKANYFLDQLAPAIGNRPIHEIEPFEVLAPLKRLEATGK
HETAKKCRSFAGRVFRYGVATTRCKSDPTSMLKGALITPKATHYAAILEPKELGGLLRAIDDYTGYMVTKFALQIAPHVF
VRPGELRHAEWREVDLVDGVWKIPAGKMKARRAHAVPLSKQVISYLTDLAEMLGHEGYVFPSARTSKRPMSENTLNAAFR
RMGYSKDEVTAHGLRATASTFLNESGLWNPDAIERALAHGDSNAVRGIYHRGKHWDERVRMAQWWSDYLDELRSGGKVIE
AKFARG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=391, Percent_Identity=34.2710997442455, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI145693166, Length=399, Percent_Identity=35.0877192982456, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788974, Length=408, Percent_Identity=32.1078431372549, Blast_Score=202, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46069; Mature: 45937

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTETRLRALKKKTKSYKVADQRGLYIEVTPAGSKLWRFRYRIGKVEKKLAIGSYPEIS
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LKEARDVTFEARQAVASGRDPAFEKRKQKIREEFLSAQTFETVAREYIEQMMVQNGRAEA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TIVKANYFLDQLAPAIGNRPIHEIEPFEVLAPLKRLEATGKHETAKKCRSFAGRVFRYGV
EEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ATTRCKSDPTSMLKGALITPKATHYAAILEPKELGGLLRAIDDYTGYMVTKFALQIAPHV
HHHHCCCCHHHHHCCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FVRPGELRHAEWREVDLVDGVWKIPAGKMKARRAHAVPLSKQVISYLTDLAEMLGHEGYV
EECCCCCCCCCCCCCHHHCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
FPSARTSKRPMSENTLNAAFRRMGYSKDEVTAHGLRATASTFLNESGLWNPDAIERALAH
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
GDSNAVRGIYHRGKHWDERVRMAQWWSDYLDELRSGGKVIEAKFARG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
>Mature Secondary Structure 
PLTETRLRALKKKTKSYKVADQRGLYIEVTPAGSKLWRFRYRIGKVEKKLAIGSYPEIS
CCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LKEARDVTFEARQAVASGRDPAFEKRKQKIREEFLSAQTFETVAREYIEQMMVQNGRAEA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TIVKANYFLDQLAPAIGNRPIHEIEPFEVLAPLKRLEATGKHETAKKCRSFAGRVFRYGV
EEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ATTRCKSDPTSMLKGALITPKATHYAAILEPKELGGLLRAIDDYTGYMVTKFALQIAPHV
HHHHCCCCHHHHHCCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FVRPGELRHAEWREVDLVDGVWKIPAGKMKARRAHAVPLSKQVISYLTDLAEMLGHEGYV
EECCCCCCCCCCCCCHHHCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
FPSARTSKRPMSENTLNAAFRRMGYSKDEVTAHGLRATASTFLNESGLWNPDAIERALAH
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
GDSNAVRGIYHRGKHWDERVRMAQWWSDYLDELRSGGKVIEAKFARG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]