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The map label for this gene is metK

Identifier: 85375684

GI number: 85375684

Start: 2869315

End: 2870526

Strand: Direct

Name: metK

Synonym: ELI_14285

Alternate gene names: 85375684

Gene position: 2869315-2870526 (Clockwise)

Preceding gene: 85375683

Following gene: 85375685

Centisome position: 94.0

GC content: 63.28

Gene sequence:

>1212_bases
ATGCGCAGCACCTATCTCTTTACCTCCGAAAGCGTTTCCGAAGGTCATCCCGACAAGGTCTCGGACCAGATTTCCGATGC
CATCGTCGATCTCTTCATCGGCAAGGACCCGGAAGCGCGAGTCGCCTGCGAGACGCTGACGACTACCGAGCGCGTCATCC
TCGCTGGGGAAATCCGCGGCCAGGGGATCATGGACACCGATGGCAATTGGGCGCCAGGGATCGAGGACGAGATCGAAGCG
GCGGTGCGCCAGACGGTCAAGGAGATCGGTTACGAACAGGACGGCTTCCACTGGGAAACCTTGACCTTCGAGAATCACCT
GCATGGCCAGAGCGCGCATATCGCGCAGGGCGTGGACGCCAGCGGCAACAAGGACGAGGGCGCAGGCGACCAGGGCATCA
TGTTCGGTTTCGCCTGCGACGAAACACCCGACCTGATGCCCGCGCCGATCGACTACAGCCACAAGATCCTCCACCGGCTG
GCGACCGACCGCAAGAACGGCAATGCACCGTTTCTCGAACCAGACAGCAAGAGCCAGGTCACGCTCCGCTACGAGAATGG
CAAGCCGGTTGCTTGCACTGCAGTGGTTGTCTCTACCCAGCATGGCGCGGGCTATGACGAAGGCGAGAAGGAAGCCGAAC
TGCGCGCCTATGTGAAAAGAGTGGTCGCGGATATCCTCCCGGACGGGTTCCTGTCGGACGCCACCAAATGGCACATCAAC
CCGACCGGCACTTTCGTGATCGGCGGTCCGGACGGCGATGCCGGCCTTACCGGGCGCAAGATAATCGTCGACACCTATGG
CGGTGCGGCCCCGCACGGGGGCGGCGCGTTCAGCGGCAAGGACCCGACCAAGGTCGATCGTTCGGCCGCCTATATTACCC
GCTATCTCGCCAAGAATGTCGTTGCGGCAGGTCTGGCGACGCGCTGCACGATCCAGATCGCTTACGCGATCGGGGTCAGC
CAGCCGCTCTCGCTCTATGTCGATACGCACGGCACCGGCACGGTGGACGACGCCGCGATCGAGGAGGCGATCCTCGATAT
CGAGAAGCTCGGCGGGCTGACCCCGCGCGCCATCCGCACGCATCTCGGGCTCAACAAGCCGATCTACCGCGTGTCGGCGG
CCTACGGTCATTTTGGGCGCACGGCGGAGGGCGATCGTTTCCCGTGGGAGCGGACGGATTTGGTCGAAGACCTGAAGGCA
GCGCTCGCCTGA

Upstream 100 bases:

>100_bases
GGCGCGCTGGTTGCCATGCGTTGGCGCGCTCGCTAGGACTGCGGCAAATAGACATAAAGATTCCTTTATATGTTCGGAAT
CGCTCTGCATTTGAGGGACC

Downstream 100 bases:

>100_bases
CCTGCTAGGGGCGCAGCATGATCGCGCCTCCATCTTCGGCAACCCACCGTATCGTGCAGGTCGACGCTTTGCGCGGGCTC
GCGGTGATGGGCATCGCCTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MRSTYLFTSESVSEGHPDKVSDQISDAIVDLFIGKDPEARVACETLTTTERVILAGEIRGQGIMDTDGNWAPGIEDEIEA
AVRQTVKEIGYEQDGFHWETLTFENHLHGQSAHIAQGVDASGNKDEGAGDQGIMFGFACDETPDLMPAPIDYSHKILHRL
ATDRKNGNAPFLEPDSKSQVTLRYENGKPVACTAVVVSTQHGAGYDEGEKEAELRAYVKRVVADILPDGFLSDATKWHIN
PTGTFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYITRYLAKNVVAAGLATRCTIQIAYAIGVS
QPLSLYVDTHGTGTVDDAAIEEAILDIEKLGGLTPRAIRTHLGLNKPIYRVSAAYGHFGRTAEGDRFPWERTDLVEDLKA
ALA

Sequences:

>Translated_403_residues
MRSTYLFTSESVSEGHPDKVSDQISDAIVDLFIGKDPEARVACETLTTTERVILAGEIRGQGIMDTDGNWAPGIEDEIEA
AVRQTVKEIGYEQDGFHWETLTFENHLHGQSAHIAQGVDASGNKDEGAGDQGIMFGFACDETPDLMPAPIDYSHKILHRL
ATDRKNGNAPFLEPDSKSQVTLRYENGKPVACTAVVVSTQHGAGYDEGEKEAELRAYVKRVVADILPDGFLSDATKWHIN
PTGTFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYITRYLAKNVVAAGLATRCTIQIAYAIGVS
QPLSLYVDTHGTGTVDDAAIEEAILDIEKLGGLTPRAIRTHLGLNKPIYRVSAAYGHFGRTAEGDRFPWERTDLVEDLKA
ALA
>Mature_403_residues
MRSTYLFTSESVSEGHPDKVSDQISDAIVDLFIGKDPEARVACETLTTTERVILAGEIRGQGIMDTDGNWAPGIEDEIEA
AVRQTVKEIGYEQDGFHWETLTFENHLHGQSAHIAQGVDASGNKDEGAGDQGIMFGFACDETPDLMPAPIDYSHKILHRL
ATDRKNGNAPFLEPDSKSQVTLRYENGKPVACTAVVVSTQHGAGYDEGEKEAELRAYVKRVVADILPDGFLSDATKWHIN
PTGTFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYITRYLAKNVVAAGLATRCTIQIAYAIGVS
QPLSLYVDTHGTGTVDDAAIEEAILDIEKLGGLTPRAIRTHLGLNKPIYRVSAAYGHFGRTAEGDRFPWERTDLVEDLKA
ALA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=395, Percent_Identity=48.1012658227848, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI4557737, Length=395, Percent_Identity=47.5949367088608, Blast_Score=320, Evalue=1e-87,
Organism=Escherichia coli, GI1789311, Length=401, Percent_Identity=52.1197007481297, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551082, Length=399, Percent_Identity=47.3684210526316, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17538498, Length=394, Percent_Identity=46.4467005076142, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17538494, Length=394, Percent_Identity=46.4467005076142, Blast_Score=321, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI25145633, Length=409, Percent_Identity=45.2322738386308, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI32565905, Length=392, Percent_Identity=42.8571428571429, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32565911, Length=347, Percent_Identity=43.8040345821326, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI25145635, Length=359, Percent_Identity=42.8969359331476, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI32565903, Length=304, Percent_Identity=46.3815789473684, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI32565909, Length=88, Percent_Identity=47.7272727272727, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323209, Length=399, Percent_Identity=47.1177944862155, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6320710, Length=400, Percent_Identity=46, Blast_Score=313, Evalue=4e-86,
Organism=Drosophila melanogaster, GI24580527, Length=402, Percent_Identity=45.273631840796, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24580525, Length=402, Percent_Identity=45.273631840796, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI45552159, Length=402, Percent_Identity=45.0248756218905, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24580529, Length=402, Percent_Identity=45.0248756218905, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24580537, Length=402, Percent_Identity=45.0248756218905, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24580533, Length=402, Percent_Identity=45.0248756218905, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24580535, Length=402, Percent_Identity=45.0248756218905, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24580531, Length=402, Percent_Identity=45.0248756218905, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24580541, Length=401, Percent_Identity=43.142144638404, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24580539, Length=132, Percent_Identity=45.4545454545455, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_ERYLH (Q2N5U2)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459746.1
- ProteinModelPortal:   Q2N5U2
- SMR:   Q2N5U2
- STRING:   Q2N5U2
- GeneID:   3870129
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_14285
- NMPDR:   fig|314225.3.peg.161
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   QDGFHWK
- ProtClustDB:   PRK05250
- BioCyc:   ELIT314225:ELI_14285-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43298; Mature: 43298

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSTYLFTSESVSEGHPDKVSDQISDAIVDLFIGKDPEARVACETLTTTERVILAGEIRG
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCC
QGIMDTDGNWAPGIEDEIEAAVRQTVKEIGYEQDGFHWETLTFENHLHGQSAHIAQGVDA
CCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCC
SGNKDEGAGDQGIMFGFACDETPDLMPAPIDYSHKILHRLATDRKNGNAPFLEPDSKSQV
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
TLRYENGKPVACTAVVVSTQHGAGYDEGEKEAELRAYVKRVVADILPDGFLSDATKWHIN
EEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEC
PTGTFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYITRYLAKNV
CCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VAAGLATRCTIQIAYAIGVSQPLSLYVDTHGTGTVDDAAIEEAILDIEKLGGLTPRAIRT
HHHCHHHEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
HLGLNKPIYRVSAAYGHFGRTAEGDRFPWERTDLVEDLKAALA
HCCCCCCHHEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSTYLFTSESVSEGHPDKVSDQISDAIVDLFIGKDPEARVACETLTTTERVILAGEIRG
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCC
QGIMDTDGNWAPGIEDEIEAAVRQTVKEIGYEQDGFHWETLTFENHLHGQSAHIAQGVDA
CCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCC
SGNKDEGAGDQGIMFGFACDETPDLMPAPIDYSHKILHRLATDRKNGNAPFLEPDSKSQV
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
TLRYENGKPVACTAVVVSTQHGAGYDEGEKEAELRAYVKRVVADILPDGFLSDATKWHIN
EEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEC
PTGTFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYITRYLAKNV
CCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VAAGLATRCTIQIAYAIGVSQPLSLYVDTHGTGTVDDAAIEEAILDIEKLGGLTPRAIRT
HHHCHHHEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
HLGLNKPIYRVSAAYGHFGRTAEGDRFPWERTDLVEDLKAALA
HCCCCCCHHEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA