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The map label for this gene is btuB [H]

Identifier: 85374988

GI number: 85374988

Start: 2187721

End: 2189652

Strand: Reverse

Name: btuB [H]

Synonym: ELI_10805

Alternate gene names: 85374988

Gene position: 2189652-2187721 (Counterclockwise)

Preceding gene: 85374990

Following gene: 85374987

Centisome position: 71.74

GC content: 64.18

Gene sequence:

>1932_bases
ATGAAATACGTTCATTTATTGCTTCTGTCTTCCGCATTCCTGACAAGTCCGGCGCTCGCGCAAACGATCGATCCCGATGC
CGATCCTGAAACCTCCACGACCTCGATCGTCGTTACCGGGAACCGCGCGACCGATCCCGTGCCGATCGATCAGGTTGGCG
GATCGGTCACGATCCTCGACCAGCAGGTCCTCGATCGGCGGCAAGTGCGCGAGGTGTCCGACGTGCTGCGCGACGTCCCG
GGTGTCGCGGTCGGGCGGGTTCCCGGCCAGACGCAGATCCGGCTGCGAGGGGCCGAAGCCAATCATACGCTCGTCCTGAT
CGATGGGATCGAGGTGTCCGACCCCTACGCTGGCGAATTCGATTTCGGAACGCTCATCGCCGACGAGGCGGCGCGGATCG
AGGTGCTGCGCGGGCAGCAGAGCGCGATCTACGGATCGGACGCGATCGGCGGCGTCATCCAGTACATCACGCTCGGCGGC
CGCGATGCTCCCGGGGCGGCCGCGCGTATCGAAGCCGGTTCGTTCGGCACGCTGAACGGTGCTGCCCGGATCGCCGGATA
TAGCGGCGATCTCGACTATGCGCTGTCCGCCACGCTCAACACCACCGATGGCACGCCAGGCGCCCGCGGCGGCACGCGCG
ACCTTGCGAACGACAGCGGTGCGGTGTCTCTCAAGACGACGTTCGCGCCTACGTCCGATGCACGCCTTACCGGCGTATTG
CGCTACGCACGCACCGATGCCGAATTCAACGACAGCGATTCCGATCCGGCAAGCCCGACGTTCGGATTCCAGGTCGATAG
CCCCGGCAACCGTTTCCAGAACGAGGCTGTCTACGCGCTGGTGCGGGGCGAGGTGGACCTGCTCGACGATCGTTGGACCC
ATGCGCTGTCGGCACAGATCGCGGACACCCGGCGCGACGGTTTCGCTTCGACTGGACGCAGCTACGGCAGTGAAGGGCAG
AGGCTGAAAGGGTCCTACGATACGACGTTGCGTATCGAAGCTGGCGGTCTCACACACCGGGTCACGGCGGCTCTCGACGT
CGAACGCGAACGGTTCCGTAACACCGATCCGACCGGCTTCGCATTTACCGGTCGTCGGCAGATCGACAATATCGGCCTCG
TCGGCCAGTATGCGATCGATCTGGGAGACCGGGCGAGCGCGTCCGCCTCGATTCGCAGGGACTGGAACGACCAGTTCGCC
GATACGACGACCTACAGACTTCAGGCGAGCTATCGGATCGCTGCGGCTACGCGGGTGCGCGGGGCAGCAGGATCGGGGGT
GAAGAACCCCTCGTTCTACGAACTCTACGGTTTCGTCGACGGTCGCTTTATAGGCAATCCCGACTTGAGGCCCGAGAAGT
CGAGCGGATGGGAGATCGGGATAGACCAATCGCTGTTCGGTGAGCGCGCTTCGATTGGGGTCACCTATTTCGAAAGCGAA
CTGGACGACGAGATCTTCACTTCCTTTCCGCCCCCGAACTTCGTCGCCAGCCCCAGCAATCGCGACACCGTCTCTGAAAG
AAACGGTGTCGAAGTGTCCGGCGAGGCGCGCTTGGGCGAGGCCTGGCGCGTCAATGGAGCCTACACCTATCTGCGTTCCC
GCGAGAATGGCACGCGCGAGGTACGTCGGCCCTCGCATACCGCCAGTCTGGCCGTCGACTGGCGCGTTCCCGGGGATGCG
GGAGGGGTCACGCTCGTCGCGCGCTACAATGGCGAGACGATCGACAGCGCCTTCATCGATCCCTCCTTCGTGCCGGTTCG
CGTGCAACTGGACGACTACCTGCTGCTGAACGTCGCCGCCGACGTGAGGGTCATGGATAATGTCGAGCTGTTCGGCCGGG
TCGAGAACCTGACGGCCGCCGACTACGAGGATGTCTTCAGCTTCGCGACGCCGGGACGCTCGTTCGCCGTCGGCGCGCGC
GCACGCTTCTAG

Upstream 100 bases:

>100_bases
ACCGCCGGGGGATGCACGCGTCCGTCCCCTGGCAGTCTCGTCGACCGCGTTTGCGCGGACGCTCCGGCCAAGTCGCCTGG
CGCCCTGGAAATTCGTGTCT

Downstream 100 bases:

>100_bases
GGAGAATTTCGATGACGGATGAAATCACGGCGCAGTCGGAGGCTGCTAGGTTCGCATGGCCTGTCACACTCGCCTTGGCG
ACCGTGGGCGGATCGCTGGC

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MKYVHLLLLSSAFLTSPALAQTIDPDADPETSTTSIVVTGNRATDPVPIDQVGGSVTILDQQVLDRRQVREVSDVLRDVP
GVAVGRVPGQTQIRLRGAEANHTLVLIDGIEVSDPYAGEFDFGTLIADEAARIEVLRGQQSAIYGSDAIGGVIQYITLGG
RDAPGAAARIEAGSFGTLNGAARIAGYSGDLDYALSATLNTTDGTPGARGGTRDLANDSGAVSLKTTFAPTSDARLTGVL
RYARTDAEFNDSDSDPASPTFGFQVDSPGNRFQNEAVYALVRGEVDLLDDRWTHALSAQIADTRRDGFASTGRSYGSEGQ
RLKGSYDTTLRIEAGGLTHRVTAALDVERERFRNTDPTGFAFTGRRQIDNIGLVGQYAIDLGDRASASASIRRDWNDQFA
DTTTYRLQASYRIAAATRVRGAAGSGVKNPSFYELYGFVDGRFIGNPDLRPEKSSGWEIGIDQSLFGERASIGVTYFESE
LDDEIFTSFPPPNFVASPSNRDTVSERNGVEVSGEARLGEAWRVNGAYTYLRSRENGTREVRRPSHTASLAVDWRVPGDA
GGVTLVARYNGETIDSAFIDPSFVPVRVQLDDYLLLNVAADVRVMDNVELFGRVENLTAADYEDVFSFATPGRSFAVGAR
ARF

Sequences:

>Translated_643_residues
MKYVHLLLLSSAFLTSPALAQTIDPDADPETSTTSIVVTGNRATDPVPIDQVGGSVTILDQQVLDRRQVREVSDVLRDVP
GVAVGRVPGQTQIRLRGAEANHTLVLIDGIEVSDPYAGEFDFGTLIADEAARIEVLRGQQSAIYGSDAIGGVIQYITLGG
RDAPGAAARIEAGSFGTLNGAARIAGYSGDLDYALSATLNTTDGTPGARGGTRDLANDSGAVSLKTTFAPTSDARLTGVL
RYARTDAEFNDSDSDPASPTFGFQVDSPGNRFQNEAVYALVRGEVDLLDDRWTHALSAQIADTRRDGFASTGRSYGSEGQ
RLKGSYDTTLRIEAGGLTHRVTAALDVERERFRNTDPTGFAFTGRRQIDNIGLVGQYAIDLGDRASASASIRRDWNDQFA
DTTTYRLQASYRIAAATRVRGAAGSGVKNPSFYELYGFVDGRFIGNPDLRPEKSSGWEIGIDQSLFGERASIGVTYFESE
LDDEIFTSFPPPNFVASPSNRDTVSERNGVEVSGEARLGEAWRVNGAYTYLRSRENGTREVRRPSHTASLAVDWRVPGDA
GGVTLVARYNGETIDSAFIDPSFVPVRVQLDDYLLLNVAADVRVMDNVELFGRVENLTAADYEDVFSFATPGRSFAVGAR
ARF
>Mature_643_residues
MKYVHLLLLSSAFLTSPALAQTIDPDADPETSTTSIVVTGNRATDPVPIDQVGGSVTILDQQVLDRRQVREVSDVLRDVP
GVAVGRVPGQTQIRLRGAEANHTLVLIDGIEVSDPYAGEFDFGTLIADEAARIEVLRGQQSAIYGSDAIGGVIQYITLGG
RDAPGAAARIEAGSFGTLNGAARIAGYSGDLDYALSATLNTTDGTPGARGGTRDLANDSGAVSLKTTFAPTSDARLTGVL
RYARTDAEFNDSDSDPASPTFGFQVDSPGNRFQNEAVYALVRGEVDLLDDRWTHALSAQIADTRRDGFASTGRSYGSEGQ
RLKGSYDTTLRIEAGGLTHRVTAALDVERERFRNTDPTGFAFTGRRQIDNIGLVGQYAIDLGDRASASASIRRDWNDQFA
DTTTYRLQASYRIAAATRVRGAAGSGVKNPSFYELYGFVDGRFIGNPDLRPEKSSGWEIGIDQSLFGERASIGVTYFESE
LDDEIFTSFPPPNFVASPSNRDTVSERNGVEVSGEARLGEAWRVNGAYTYLRSRENGTREVRRPSHTASLAVDWRVPGDA
GGVTLVARYNGETIDSAFIDPSFVPVRVQLDDYLLLNVAADVRVMDNVELFGRVENLTAADYEDVFSFATPGRSFAVGAR
ARF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=172, Percent_Identity=37.7906976744186, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1788478, Length=156, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 69277; Mature: 69277

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVHLLLLSSAFLTSPALAQTIDPDADPETSTTSIVVTGNRATDPVPIDQVGGSVTILD
CCEEEEEEHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCEEEEEC
QQVLDRRQVREVSDVLRDVPGVAVGRVPGQTQIRLRGAEANHTLVLIDGIEVSDPYAGEF
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCEEEEEECEECCCCCCCCC
DFGTLIADEAARIEVLRGQQSAIYGSDAIGGVIQYITLGGRDAPGAAARIEAGSFGTLNG
CCCEEECCCCCEEEEECCCCCEEECCHHHHHHEEEEEECCCCCCCCEEEEECCCCCCCCC
AARIAGYSGDLDYALSATLNTTDGTPGARGGTRDLANDSGAVSLKTTFAPTSDARLTGVL
CEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHEE
RYARTDAEFNDSDSDPASPTFGFQVDSPGNRFQNEAVYALVRGEVDLLDDRWTHALSAQI
HHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCEEEECCHHHHHHHHHH
ADTRRDGFASTGRSYGSEGQRLKGSYDTTLRIEAGGLTHRVTAALDVERERFRNTDPTGF
HHHCCCCCHHCCCCCCCCCCEECCCCCEEEEEEECCCEEEEEEEEECCHHHHCCCCCCCE
AFTGRRQIDNIGLVGQYAIDLGDRASASASIRRDWNDQFADTTTYRLQASYRIAAATRVR
EEECCCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCCHHCCCEEEEEEEEEEEEEEEEEC
GAAGSGVKNPSFYELYGFVDGRFIGNPDLRPEKSSGWEIGIDQSLFGERASIGVTYFESE
CCCCCCCCCCCEEEEEEEECCEEECCCCCCCCCCCCCEECCCHHHCCCCCEEEEEEECCC
LDDEIFTSFPPPNFVASPSNRDTVSERNGVEVSGEARLGEAWRVNGAYTYLRSRENGTRE
CCCHHHHCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCCEEEECCEEEEEECCCCCCHH
VRRPSHTASLAVDWRVPGDAGGVTLVARYNGETIDSAFIDPSFVPVRVQLDDYLLLNVAA
HCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCHHCCCCCEEEEEEEECCEEEEEEEE
DVRVMDNVELFGRVENLTAADYEDVFSFATPGRSFAVGARARF
CEEEECCHHEEEEECCCCCCCHHHHHHHCCCCCCEEECCCCCC
>Mature Secondary Structure
MKYVHLLLLSSAFLTSPALAQTIDPDADPETSTTSIVVTGNRATDPVPIDQVGGSVTILD
CCEEEEEEHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCEEEEEC
QQVLDRRQVREVSDVLRDVPGVAVGRVPGQTQIRLRGAEANHTLVLIDGIEVSDPYAGEF
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCEEEEEECEECCCCCCCCC
DFGTLIADEAARIEVLRGQQSAIYGSDAIGGVIQYITLGGRDAPGAAARIEAGSFGTLNG
CCCEEECCCCCEEEEECCCCCEEECCHHHHHHEEEEEECCCCCCCCEEEEECCCCCCCCC
AARIAGYSGDLDYALSATLNTTDGTPGARGGTRDLANDSGAVSLKTTFAPTSDARLTGVL
CEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHEE
RYARTDAEFNDSDSDPASPTFGFQVDSPGNRFQNEAVYALVRGEVDLLDDRWTHALSAQI
HHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCEEEECCHHHHHHHHHH
ADTRRDGFASTGRSYGSEGQRLKGSYDTTLRIEAGGLTHRVTAALDVERERFRNTDPTGF
HHHCCCCCHHCCCCCCCCCCEECCCCCEEEEEEECCCEEEEEEEEECCHHHHCCCCCCCE
AFTGRRQIDNIGLVGQYAIDLGDRASASASIRRDWNDQFADTTTYRLQASYRIAAATRVR
EEECCCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCCHHCCCEEEEEEEEEEEEEEEEEC
GAAGSGVKNPSFYELYGFVDGRFIGNPDLRPEKSSGWEIGIDQSLFGERASIGVTYFESE
CCCCCCCCCCCEEEEEEEECCEEECCCCCCCCCCCCCEECCCHHHCCCCCEEEEEEECCC
LDDEIFTSFPPPNFVASPSNRDTVSERNGVEVSGEARLGEAWRVNGAYTYLRSRENGTRE
CCCHHHHCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCCEEEECCEEEEEECCCCCCHH
VRRPSHTASLAVDWRVPGDAGGVTLVARYNGETIDSAFIDPSFVPVRVQLDDYLLLNVAA
HCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCHHCCCCCEEEEEEEECCEEEEEEEE
DVRVMDNVELFGRVENLTAADYEDVFSFATPGRSFAVGARARF
CEEEECCHHEEEEECCCCCCCHHHHHHHCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA